Characterization of bovine oviduct epithelial cell monolayers cultured under serum-free conditions

1995 ◽  
Vol 31 (9) ◽  
pp. 664-670 ◽  
Author(s):  
A. Van Langendonckt ◽  
A. Vansteenbrugge ◽  
C. Dessy-Doize ◽  
J. E. Flechon ◽  
G. Charpigny ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Serum Free ◽  
Cell Monolayers ◽  
Oviduct Epithelial Cell ◽  
Epithelial Cell Monolayers ◽  
Serum Free Conditions

The interaction of fresh and frozen-thawed ram spermatozoa with oviducal epithelial cells in vitro

2000 ◽  
Vol 12 (6) ◽  
pp. 237 ◽  
Author(s):  
L. Gillan ◽  
G. Evans ◽  
W. M. C. Maxwell
Keyword(s):  
Epithelial Cell ◽  
Epithelial Cells ◽  
Small Proportion ◽  
Oestrous Cycle ◽  
Cell Monolayers ◽  
Oviduct Epithelial Cell ◽  
Epithelial Cell Monolayers ◽  
Oviduct Epithelial Cells

In order to investigate the interaction of fresh and frozen–thawed spermatozoa with oviduct epithelial cells, spermatozoa were co-incubated with ovine oviduct epithelial cell monolayers (OECM) derived from either complete oviducts, at any stage of the oestrous cycle (Experiments 1 and 2), or from different regions of the oviduct at different stages of the cycle (Experiment 3). Fresh and frozen—thawed spermatozoa displayed different patterns of binding to, and release from, the OECM. Frozen—thawed spermatozoa immediately bound to the complete oviduct OECM and were released after 2 h. A small proportion of fresh spermatozoa bound immediately, increasing to a maximum after 2 h, and were gradually released thereafter. When only the cells that were released from the OECM were observed by chlortetracycline staining in Experiment 2, it was found that the presence of an OECM increased the number of capacitated fresh spermatozoa while decreasing the number of capacitated frozen–thawed spermatozoa. Overall, the OECM advanced the membrane state of both types of spermatozoa from uncapacitated to acrosome-reacted. Fresh and frozen—thawed spermatozoa bound to OECM derived from the cells of the isthmus and the ampulla in similar proportions. However, more spermatozoa were capacitated when incubated with OECM derived from isthmic rather than ampullary cells. Higher proportions of fresh spermatozoa bound to, and were acrosome-reacted following incubation with OECM derived from post- rather than pre-ovulatory tracts. Such differences were not observed for frozen—thawed spermatozoa. The findings reported in this study show that fresh and frozen—thawed spermatozoa behave differently when in contact with oviduct cells in vitro. This may be a consequence of the more advanced membrane state of the frozen spermatozoa upon thawing.

scholarly journals Human sperm function in co-culture with human, macaque or bovine oviduct epithelial cell monolayers

1998 ◽  
Vol 13 (10) ◽  
pp. 2797-2804 ◽  
Author(s):  
J.E. Ellington ◽  
A.E. Jones ◽  
C.M. Davitt ◽  
C.S. Schneider ◽  
R.S. Brisbois ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Human Sperm ◽  
Sperm Function ◽  
Cell Monolayers ◽  
Oviduct Epithelial Cell ◽  
Epithelial Cell Monolayers

scholarly journals Characteristics of Passive Solute Transport across Primary Rat Alveolar Epithelial Cell Monolayers

Membranes ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 331
Author(s):  
Yong Ho Kim ◽  
Kwang-Jin Kim ◽  
David Z. D’Argenio ◽  
Edward D. Crandall
Keyword(s):  
Epithelial Cell ◽  
Tight Junctions ◽  
Pore Radius ◽  
Alveolar Epithelial Cell ◽  
Alveolar Epithelial Cells ◽  
Type I ◽  
Cell Monolayers ◽  
Alveolar Epithelial ◽  
Epithelial Cell Monolayers ◽  
Hydrophilic Solutes

Primary rat alveolar epithelial cell monolayers (RAECM) were grown without (type I cell-like phenotype, RAECM-I) or with (type II cell-like phenotype, RAECM-II) keratinocyte growth factor to assess passive transport of 11 hydrophilic solutes. We estimated apparent permeability (Papp) in the absence/presence of calcium chelator EGTA to determine the effects of perturbing tight junctions on “equivalent” pores. Papp across RAECM-I and -II in the absence of EGTA are similar and decrease as solute size increases. We modeled Papp of the hydrophilic solutes across RAECM-I/-II as taking place via heterogeneous populations of equivalent pores comprised of small (0.41/0.32 nm radius) and large (9.88/11.56 nm radius) pores, respectively. Total equivalent pore area is dominated by small equivalent pores (99.92–99.97%). The number of small and large equivalent pores in RAECM-I was 8.55 and 1.29 times greater, respectively, than those in RAECM-II. With EGTA, the large pore radius in RAECM-I/-II increased by 1.58/4.34 times and the small equivalent pore radius increased by 1.84/1.90 times, respectively. These results indicate that passive diffusion of hydrophilic solutes across an alveolar epithelium occurs via small and large equivalent pores, reflecting interactions of transmembrane proteins expressed in intercellular tight junctions of alveolar epithelial cells.

Sign in / Sign up

Export Citation Format

Share Document