scholarly journals 15-Hydroxyprostaglandin dehydrogenase in the bovine endometrium during the oestrous cycle and early pregnancy

Reproduction ◽  
2006 ◽  
Vol 131 (3) ◽  
pp. 573-582 ◽  
Author(s):  
Marianne Parent ◽  
Eric Madore ◽  
Leslie A MacLaren ◽  
Michel A Fortier
Keyword(s):  
Early Pregnancy ◽  
Critical Period ◽  
Reproductive Function ◽  
Oestrous Cycle ◽  
Maximal Level ◽  
Relative Production ◽  
High Level ◽  
Human Isoforms ◽  
Luminal Epithelial Cells ◽  
Viable Embryo

Prostaglandins (PG) are primary regulators of reproductive function. In ruminants, the relative production of PGE2and PGF2αdetermines the return to a new oestrous cycle or to the establishment of pregnancy in response to a viable embryo. PG action depends on biosynthesis, transport and interaction with their receptors, which are all expressed differentially during the oestrous cycle. PGs are, however, local mediators and thus the onsite degradation by enzymes such as 15-hydroxyprostaglandin dehydrogenase (HPGD), also known as 15-PGDH, is another factor to consider in the regulation of physiological action. Little information is available on PG catabolism in the endometrium during the oestrous cycle or early pregnancy. The purpose of this study was to clone the bovine 15-PGDH, produce the recombinant protein and generate a specific antibody to study its activity and its expression in the endometrium during the oestrous cycle. We have found that the bovine 15-PGDH is highly homologous to the rat and human isoforms. 15-PGDH is localized principally in the glandular epithelium and to a lesser extent in stromal and luminal epithelial cells. The enzyme expression is regulated during the oestrous cycle and it reaches its maximal level on days 16–18. Transient expression is observed in luminal epithelial and trophoblast cells on day 21 of pregnancy. The mRNA is expressed at a constant high level throughout the cycle. The activity of the recombinant 15-PGDH was also tested and was found comparable for PGF2αand PGE2. These data suggest that 15-PGDH contributes to the tight regulation of PG action in the endometrium especially at the critical period of recognition of pregnancy.

scholarly journals Immunohistochemical studies on the progesterone receptor (PR) in the sow uterus during the oestrous cycle and in inseminated sows at oestrus and early pregnancy

Reproduction ◽  
2005 ◽  
Vol 129 (3) ◽  
pp. 349-359 ◽  
Author(s):  
S Sukjumlong ◽  
A-M Dalin ◽  
L Sahlin ◽  
E Persson
Keyword(s):  
Monoclonal Antibody ◽  
Progesterone Receptor ◽  
Early Pregnancy ◽  
Artificial Insemination ◽  
Oestrous Cycle ◽  
Surface Epithelium ◽  
Physiological Changes ◽  
Receptor Proteins ◽  
High Level ◽  
Immunohistochemical Studies

Physiological changes in the sow uterus involve the regulation by progesterone and its receptor proteins (PR). Therefore, the aim of the present study was to investigate the localization of PR during different stages of the oestrous cycle and in inseminated sows during early pregnancy by use of immunohistochemistry. Uterine samples were collected from cyclic and inseminated sows at different stages of the oestrous cycle and early pregnancy. The samples were fixed in 10% formaldehyde and embedded in paraffin. Immunohistochemistry was done by use of a mouse monoclonal antibody to PR. The highest PR immunostaining in the surface epithelium was observed at oestrus/5–6 h after artificial insemination (AI) and early dioestrus/70 h after AI. In the glandular epithelium, the highest level of PR was found at oestrus with the lowest at late dioestrus/d 19. Higher levels of PR were observed in inseminated groups compared with cyclic sows. In the myometrium, a high level of PR was found at oestrus, while stromal PR cells were constantly present throughout the oestrous cycle and at different stages of early pregnancy. In conclusion, this study shows that the immunopresence of PR in the sow uterus differed between uterine compartments at the same reproductive stage. Differences were also found for some uterine compartments between cyclic and inseminated/early pregnant sows. The relatively consistent immunostaining of PR in the stroma strengthens a stromal role in the regulation of physiological activities in the sow uterus during the oestrous cycle as well as early pregnancy.

scholarly journals Determining the critical period for weed control in high-yielding cotton using common sunflower as a mimic weed

2019 ◽  
Vol 33 (6) ◽  
pp. 800-807 ◽  
Author(s):  
Graham W. Charles ◽  
Brian M. Sindel ◽  
Annette L. Cowie ◽  
Oliver G. G. Knox
Keyword(s):  
Weed Control ◽  
Critical Period ◽  
Yield Loss ◽  
Field Studies ◽  
Growing Degree Days ◽  
Degree Days ◽  
Weed Density ◽  
High Level ◽  
The Cost ◽  
Planting Season

AbstractField studies were conducted over six seasons to determine the critical period for weed control (CPWC) in high-yielding cotton, using common sunflower as a mimic weed. Common sunflower was planted with or after cotton emergence at densities of 1, 2, 5, 10, 20, and 50 plants m−2. Common sunflower was added and removed at approximately 0, 150, 300, 450, 600, 750, and 900 growing degree days (GDD) after planting. Season-long interference resulted in no harvestable cotton at densities of five or more common sunflower plants m−2. High levels of intraspecific and interspecific competition occurred at the highest weed densities, with increases in weed biomass and reductions in crop yield not proportional to the changes in weed density. Using a 5% yield-loss threshold, the CPWC extended from 43 to 615 GDD, and 20 to 1,512 GDD for one and 50 common sunflower plants m−2, respectively. These results highlight the high level of weed control required in high-yielding cotton to ensure crop losses do not exceed the cost of control.

scholarly journals Characterization of the Th Profile of the Bovine Endometrium during the Oestrous Cycle and Early Pregnancy

PLoS ONE ◽  
2013 ◽  
Vol 8 (10) ◽  
pp. e75571 ◽  
Author(s):  
Lilian J. Oliveira ◽  
Nadéra Mansourri-Attia ◽  
Alan G. Fahey ◽  
John Browne ◽  
Niamh Forde ◽  
...  
Keyword(s):  
Early Pregnancy ◽  
Oestrous Cycle

scholarly journals Local relaxin biosynthesis in the ovary and uterus through the oestrous cycle and early pregnancy in the female marmoset monkey (Callithrix jacchus)

1997 ◽  
Vol 12 (6) ◽  
pp. 1325-1337 ◽  
Author(s):  
A. Einspanier ◽  
M. R. Zarreh-Hoshyari-Khah ◽  
M. Balvers ◽  
L. Kerr ◽  
K. Fuhrmann ◽  
...  
Keyword(s):  
Early Pregnancy ◽  
Callithrix Jacchus ◽  
Oestrous Cycle ◽  
Marmoset Monkey

scholarly journals Expression of inter-alpha-trypsin inhibitor heavy chains in endometrium of cyclic and pregnant gilts

Reproduction ◽  
2003 ◽  
pp. 621-627 ◽  
Author(s):  
RD Geisert ◽  
MD Ashworth ◽  
Keyword(s):  
Gene Expression ◽  
Extracellular Matrix ◽  
Western Blot ◽  
Protease Inhibitor ◽  
Early Pregnancy ◽  
Trypsin Inhibitor ◽  
Serine Protease Inhibitor ◽  
Oestrous Cycle ◽  
Heavy Chains ◽  
Formation Of Complexes

Attachment of the placenta to the uterus in pigs involves extracellular interaction between the expanding trophoblastic membrane and the thick glycocalyx present on the uterine epithelial microvilli. Formation of complexes between members of inter-alpha-trypsin inhibitor family may function in the maintenance of the extracellular matrix. This study investigated the change in the inter-alpha-trypsin inhibitor heavy chains (ITIH1, ITIH2, ITIH3 and ITIH4) during the oestrous cycle and early pregnancy in pigs. Gene expression of ITIH1, ITIH2, ITIH3 and ITIH4 was detected in the endometrium of cyclic and pregnant gilts; however, gene expression of ITIH was not altered throughout the oestrous cycle or early pregnancy. Western blot analysis with an ITIH antiserum identified the possible linkage forms of ITIH with the serine protease inhibitor, bikunin. Pregnancy altered the release of the various inter-alpha-inhibitor forms from the endometrium during the period of trophoblastic attachment. The results from this study indicate that the inter-alpha-trypsin inhibitor family plays an important role in maintenance of the uterine surface glycocalyx during placental attachment in pigs.

Critical Period of Quackgrass (Elytrigia repens) Removal in Potatoes (Solanum tuberosum)

Weed Science ◽  
1994 ◽  
Vol 42 (4) ◽  
pp. 528-533 ◽  
Author(s):  
R. Baziramakenga ◽  
Gilles D. Leroux
Keyword(s):  
Critical Period ◽  
Yield Loss ◽  
Field Trials ◽  
Quebec City ◽  
Critical Periods ◽  
Marketable Yield ◽  
Elytrigia Repens ◽  
Medium Level ◽  
High Level ◽  
Loss Level

Field trials were carried out in 1989 and 1990 at St-Augustin, near Québec City, Canada, to determine the critical periods of quackgrass control in potato submitted to three levels of infestation. Potato yield losses due to quackgrass interference increased with quackgrass infestation and length of duration of interference. Quackgrass interference influenced marketable tuber yields more than total tuber yields. Duration of the critical period varied depending on the level of quackgrass infestation and year. Based on an arbitrary 5% level of marketable yield loss, the critical period started at ca. 15 days after emergence (DAE) of potato at low level of infestation, and at ca. 3 DAE at medium level of infestation. At high level of infestation, the critical period began prior to the emergence of potato. The end of the critical period of quackgrass removal was extremely variable across quackgrass infestation level and year and ranged from 23 to 68 DAE of potato at a 5 % yield loss level. It appears that onset of interference varied less than the end of it, indicating that early quackgrass control is necessary to prevent yield loss.

Sulfated glycoprotein-1 (SGP-1) expression in ovine endometrium during the oestrous cycle and early pregnancy

1995 ◽  
Vol 7 (5) ◽  
pp. 1053 ◽  
Author(s):  
TE Spencer ◽  
GH Graf ◽  
FW Bazer
Keyword(s):  
Early Pregnancy ◽  
Blot Hybridization ◽  
Immunohistochemical Localization ◽  
Oestrous Cycle ◽  
Northern Hybridization ◽  
Mrna Levels ◽  
Slot Blot ◽  
Slot Blot Hybridization ◽  
Endometrial Epithelium ◽  
Sulfated Glycoprotein

This study determined effects of day of oestrous cycle and early pregnancy on sulfated glycoprotein-1 (SGP-1) expression in ovine endometrium. A 364-bp clone of the ovine SGP-1 mRNA was amplified from reverse transcribed Day-15 cyclic endometrial mRNA using the polymerase chain reaction (PCR) and primers specific for the rat SGP-1 mRNA sequence. Nucleotide sequence of the ovine SGP-1 cDNA shared significant identity with rat SGP-1 and human prosaposin. Ewes (n = 40) were hysterectomized on either Day 1, 6, 11, 13 or 15 of the oestrous cycle or on Day 11, 13, 15, 17 or 25 of early pregnancy. Total cellular RNA was isolated from endometrium and subjected to Northern and slot blot hybridization analyses using an antisense cRNA probe transcribed from the ovine SGP-1 cDNA clone. A single 2.6-kb mRNA transcript was detected by Northern hybridization analyses. Slot blot hybridization analyses indicated that steady-state levels of endometrial SGP-1 mRNA varied during the oestrous cycle (cubic, P < 0.02) and increased between Day 11 and Day 25 of early pregnancy (linear, P < 0.01). On Days 11, 13 and 15, endometrial SGP-1 mRNA levels were greater in pregnant ewes than in cyclic ewes (day x pregnancy status, P < 0.01). Immunohistochemical localization of SGP-1 in uterine tissues with rabbit anti-rat SGP-1 antibody revealed intense immunoreactivity associated primarily with the endometrial epithelium. These results indicate that the ovine endometrium expresses SGP-1, a prosaposin, and that SGP-1 expression varies during the oestrous cycle and is enhanced by the conceptus. The presence of SGP-1 in the endometrium suggests intracellular and extracellular roles for this protein in glycosphingolipid metabolism or transport in the uterine environment.

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