scholarly journals Extracellular Ca2+-sensing receptor expression and hormonal regulation in rat uterus during the peri-implantation period

Reproduction ◽  
2005 ◽  
Vol 129 (6) ◽  
pp. 779-788 ◽  
Author(s):  
Li-Juan Xiao ◽  
Jin-Xiang Yuan ◽  
Yin-Chuan Li ◽  
Rui Wang ◽  
Zhao-Yuan Hu ◽  
...  
Keyword(s):  
Hormonal Regulation ◽  
Early Stage ◽  
Embryo Implantation ◽  
Receptor Expression ◽  
Luminal Epithelium ◽  
Rat Uterus ◽  
Delayed Implantation ◽  
Wide Range ◽  
High Level ◽  
Implantation Period

The extracellular Ca2+-sensing receptor (CaR) is a member of the superfamily of G protein-coupled receptors (GPCRs). It is an important mediator of a wide range of Ca2+-dependent physiological responses in various tissues. In reproductive tissues it has been reported to play a significant role in promoting or maintaining placentation. Meanwhile, another Ca2+regulated gene stanniocalcin-1 (STC-1) has been documented to be involved in decidualization and uterine remodelling. The phenomenon that CaR mediates STC-1’s transcription responding to extracellular calcium in fish urges us to suppose that CaR, like STC-1, may also play a role in implantation and decidualization. To resolve this conjecture, we have examined the expression and hormonal regulation of the CaR gene in rat uterus during peri-implantation period.CaR mRNA was expressed at a moderate level in the luminal epithelium of the early stage of pregnancy (from day 1 to day 3). From day 2–3 it began to be expressed more strongly in the stromal cells immediately underneath the luminal epithelium, but decreased to a basal level on day 4. From day 6 to day 9 continuously, both CaR mRNA and protein were highly expressed in the primary decidua. Expression of CaR mRNA and protein in these cells was also observed when a delayed implantation was terminated by estrogen treatment to allow the embryo implantation. In contrast, only basal level expression of the molecules was detected in the cells of animals subjected to a normal-delayed implantation or the pseudopregnant condition.Embryo transplantation experiment confirmed that CaR expression at the implantation site was induced by the implanting blastocyst. Consistent with the normal pregnant process, CaR mRNA and protein in the cells were also induced by an artificial decidualization procedure. Further experiments demonstrated that treatment of the ovariectomized rat with estrogen or/and progesterone stimulated a high level expression of CaR mRNA in the uterine epithelial and glandular epithelium. In conclusion, CaR was specifically induced during the processes of implantation and subsequent decidualization and may play a role in these processes.

scholarly journals Basigin expression and hormonal regulation in the rat uterus during the peri-implantation period

Reproduction ◽  
2002 ◽  
pp. 219-225 ◽  
Author(s):  
LJ Xiao ◽  
HL Diao ◽  
XH Ma ◽  
NZ Ding ◽  
K Kadomatsu ◽  
...  
Keyword(s):  
Expression Pattern ◽  
Hormonal Regulation ◽  
Ovariectomized Rats ◽  
Luminal Epithelium ◽  
Rat Uterus ◽  
Oestrogen Treatment ◽  
Delayed Implantation ◽  
Similar Expression Pattern ◽  
Implantation Period ◽  
Basal Concentration

Basigin is essential for fertilization and implantation. The aim of this study was to determine the expression and hormonal regulation of the basigin gene in the rat uterus during the peri-implantation period. Basigin mRNA was localized strongly in the luminal epithelium on day 1 of pregnancy and gradually decreased to a basal concentration from day 3 to day 5 of pregnancy. Basigin mRNA and protein were expressed strongly in the implanting blastocyst and primary decidua on day 6 of pregnancy. A similar expression pattern was also induced in the uterus after delayed implantation was terminated by oestrogen treatment and the embryo implanted, whereas expression was not detected during delayed implantation. Basigin expression was not detected on day 6 of pseudopregnancy. Basigin mRNA was expressed strongly in the decidua on days 7 and 8 of pregnancy. Furthermore, both basigin mRNA and protein were induced in the decidua during artificial decidualization. In addition, oestrogen stimulated strong expression of basigin mRNA in the uterine epithelium of ovariectomized rats. These findings indicate that basigin may play a role during implantation and decidualization in rats.

scholarly journals Expression and regulation of stanniocalcin 1 and 2 in rat uterus during embryo implantation and decidualization

Reproduction ◽  
2006 ◽  
Vol 131 (6) ◽  
pp. 1137-1149 ◽  
Author(s):  
Li-Juan Xiao ◽  
Jin-Xiang Yuan ◽  
Xin-Xin Song ◽  
Yin-Chuan Li ◽  
Zhao-Yuan Hu ◽  
...  
Keyword(s):  
Early Pregnancy ◽  
Embryo Implantation ◽  
Normal Pregnancy ◽  
Implantation Site ◽  
Luminal Epithelium ◽  
Rat Uterus ◽  
Delayed Implantation ◽  
Physiological Processes ◽  
Stanniocalcin 2 ◽  
Weak Expression

Stanniocalcin-1 (STC-1) is a recently discovered polypeptide hormone, while stanniocalcin-2 (STC-2) is a subsequently identified homologue of stanniocalcin-1. Although previous studies have shown that both STC-1 and -2 are involved in various physiological processes, such as ion transport, reproduction and development, their expression in the uterus and roles in implantation and early pregnancy are unclear. Here we have investigated the expression and regulation of both STC-1 and STC-2 in rat uterus during early pregnancy under various physiological conditions. We show that only basal levels of STC-1 and STC-2 mRNA were detected in the uterus from day one (D1) to day five (D5) of pregnancy. STC-2 immunostaining was gradually increased in the glandular epithelium from day two (D2), with a peak occurring on D5. High levels of both STC-1 and STC-2 mRNA were observed in the stoma cells at the implantation site on day six (D6) of pregnancy, whereas their immunostaining signals were also significant in the luminal epithelium. Basal levels of both STC-1 and STC-2 mRNA and STC-1 immunostaining were detected in the uterus with delayed implantation. After the delayed implantation was terminated by estrogen treatment, both STC-1 and STC-2 mRNA signals were significantly induced in the stroma underlying the luminal epithelium at the implantation site, and STC-2 immunostaining was also observed in the luminal epithelium surrounding the implanting blastocyst. Embryo transfer experiments further confirmed that STC-1 and STC-2 expression at the implantation sites was induced by the implanting blastocyst. Both STC-1 mRNA and immunostaining were seen in the decidualized cells from day seven (D7) to day nine (D9) of pregnancy. STC-2 mRNA was also found in the whole decidua from D7 to D9 of pregnancy; STC-2 protein, however, was strictly localized to the primary deciduas on D7 and D8, with a weak expression in the whole deciduas on D9. Consistent with the normal pregnancy process, strong STC-1 and STC-2 mRNA signals were detected in the decidualized cells under artificial decidualization, whereas only basal levels of STC-1 mRNA and immunostaining were observed in the control horn. These data suggest, for the first time, that STC-1 together with STC-2 may play important roles in the processes of implantation and decidualization in the rat.

Expression and regulation of lanosterol 14α-demethylase in mouse embryo and uterus during the peri-implantation period

2008 ◽  
Vol 20 (8) ◽  
pp. 964 ◽  
Author(s):  
Xiaoming Song ◽  
Ping Tai ◽  
Jun Yan ◽  
Baoshan Xu ◽  
Xiufen Chen ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Mouse Embryo ◽  
Cholesterol Biosynthesis ◽  
Embryo Implantation ◽  
Preimplantation Embryos ◽  
Oestrogen Treatment ◽  
Delayed Implantation ◽  
Progesterone Treatment ◽  
High Level ◽  
Implantation Period

Lanosterol 14α-demethylase (LDM) is expressed ubiquitously in all mammals and is important in cholesterol biosynthesis. However, whether LDM expression is involved in the interaction between uterus and embryo during implantation remains unknown. In the present study, the expression of LDM was investigated in mouse embryo and uterus during the peri-implantation period using confocal microscopy, immunohistochemistry and western blot methods. Further, regulation of LDM expression was investigated in pseudopregnancy, delayed implantation, artificial decidualisation and ovariectomisation using 17β-oestradiol and progesterone treatment mouse models. The results showed that LDM was selectively expressed in preimplantation embryos and the uterine subluminal stroma surrounding the implanting blastocyst on Day 5 of pregnancy. No corresponding signal was detected in the uterus on Day 5 of pseudopregnancy. Most notably, once delayed implantation was terminated by oestrogen treatment and the embryo implanted, a high level of LDM expression was induced in the subluminal stroma surrounding the implanting blastocyst, whereas no corresponding signal was detected in the delayed implantation uterus. A high level of LDM expression was observed in the uterus decidua on Days 6–8 of pregnancy. Furthermore, LDM expression was induced in the uterine stroma under artificial decidualisation. Oestrogen, but not progesterone, treatment induced a high level of LDM expression in the uterus of ovariectomised mice. These results indicate that LDM is closely related to mouse embryo implantation and can be upregulated by oestrogen.

scholarly journals Differential expression and regulation of Cryab in mouse uterus during preimplantation period

Reproduction ◽  
2013 ◽  
Vol 145 (6) ◽  
pp. 577-585 ◽  
Author(s):  
Xue-Chao Tian ◽  
Qu-Yuan Wang ◽  
Dang-Dang Li ◽  
Shou-Tang Wang ◽  
Zhan-Qing Yang ◽  
...  
Keyword(s):  
Real Time ◽  
Stromal Cells ◽  
Real Time Pcr ◽  
Embryo Implantation ◽  
Mouse Uterus ◽  
High Level ◽  
Implantation Period

The aim of this study was to examine the expression and regulation of the crystallin, alpha B (Cryab) gene in mouse uterus during the peri-implantation period by in situ hybridization and real-time PCR. There was no detectable Cryab mRNA signal on days 1–4 of pregnancy. On day 5 of pregnancy when embryo implanted, a high level of Cryab mRNA signal was found in the subluminal stroma surrounding the implanting blastocyst. On days 6–8, Cryab mRNA was strongly expressed in the primary decidua. By real-time PCR, a high level of Cryab expression was detected on days 7 and 8 of pregnancy, although Cryab expression was seen from days 1 to 8. Under in vivo and in vitro artificial decidualization, Cryab expression was significantly elevated. Compared with the progesterone-primed delayed implantation uterus, a high level of Cryab mRNA expression was observed in estrogen-activated implantation uterus. In the uterine stromal cells, cAMP, estrogen, and progesterone could induce the expression of Cryab gene. In the ovariectomized mouse uterus, estrogen could also induce the expression of Cryab while progesterone inhibited its expression. Our data suggest that Cryab may play an important role during mouse embryo implantation and decidualization and that estrogen and progesterone can regulate the expression of Cryab gene.

scholarly journals Increased Expression of Phosphorylation of AKT in Rat Uterus During Peri-Implantation Period and Its Significance in Embryo Implantation.

2009 ◽  
Vol 81 (Suppl_1) ◽  
pp. 401-401
Author(s):  
Jinxiang Yuan ◽  
Guangqiang Zhao ◽  
Xuesen Zhang ◽  
Lijuan Xiao ◽  
Xuan Jin ◽  
...  
Keyword(s):  
Embryo Implantation ◽  
Rat Uterus ◽  
Implantation Period

scholarly journals Signal transducer and activator of transcription 3 (Stat3) expression and activation in rat uterus during early pregnancy

Reproduction ◽  
2004 ◽  
Vol 128 (2) ◽  
pp. 197-205 ◽  
Author(s):  
Chun-Bo Teng ◽  
Hong-Lu Diao ◽  
Hong Ma ◽  
Jing Cong ◽  
Hao Yu ◽  
...  
Keyword(s):  
Early Pregnancy ◽  
Stromal Cells ◽  
Embryo Implantation ◽  
Luminal Epithelium ◽  
Signal Transducer ◽  
Uterine Receptivity ◽  
Rat Uterus ◽  
Stat3 Phosphorylation ◽  
Decidual Cells ◽  
Transcription 3

Signal transducer and activator of transcription 3 (Stat3), a member of the Stat family, is specifically activated during mouse embryo implantation. The aim of this study was to investigate the expression, activation and regulation of Stat3 in rat uterus during early pregnancy, pseudopregnancy, delayed implantation and artificial decidualization. Stat3 mRNA was highly expressed in the luminal epithelium on day 5 and in the luminal epithelium and underlying stromal cells at implantation sites on day 6 of pregnancy. There was a strong level of Stat3 protein expression and phosphorylation in the stromal cells near the lumen and in the luminal epithelium on day 5 of pregnancy, which was similar to day 5 of pseudopregnancy. In the afternoon of day 6, the strong level of Stat3 phosphorylation was detected only in the luminal epithelium. Stat3 was highly expressed and activated in the decidual cells from days 7 to 9 of pregnancy and under artificial decidualization in the present study. Our results suggest that the strong level of Stat3 activation in the luminal epithelium and underlying stromal cells during the pre-implantation period may be important for establishing uterine receptivity as in mice, and the high level of Stat3 expression and activation in decidual cells may play a role during decidualization.

scholarly journals Differential expression and regulation of cylooxygenases, prostaglandin E synthases and prostacyclin synthase in rat uterus during the peri-implantation period

Reproduction ◽  
2006 ◽  
Vol 131 (1) ◽  
pp. 139-151 ◽  
Author(s):  
Jing Cong ◽  
Hong-Lu Diao ◽  
Yue-Chao Zhao ◽  
Hua Ni ◽  
Yun-Qin Yan ◽  
...  
Keyword(s):  
Implantation Site ◽  
Prostaglandin E ◽  
Luminal Epithelium ◽  
Prostaglandin I2 ◽  
Rat Uterus ◽  
Pregnant Rats ◽  
Cox 2 ◽  
Prostacyclin Synthase ◽  
Implantation Period ◽  
Cox 1

It has been shown that both prostaglandin I2 (PGI2) and PGE2 are essential for mouse implantation, whereas only PGE2 is required for hamster implantation. To date, the expression and regulation of cyclooxygenase (COX) and prostaglandin E synthase (PGES), which are responsible for PGE2 production, have not been reported in the rat. The aim of this study was to examine the expression pattern and regulation of COX-1, COX-2, membrane-associated PGES-1 (mPGES-1), mPGES-2 and cytosolic PGES (cPGES) in rat uterus during early pregnancy and pseudopregnancy, and under delayed implantation. At implantation site on day 6 of pregnancy, COX-1 immunostaining was highly visible in the luminal epithelium, and COX-2 immunostaining was clearly observed in the subluminal stroma. Both mPGES-1 mRNA and protein were only observed in the subluminal stroma surrounding the implanting blastocyst at the implantation site on day 6 of pregancy , but were not seen in the inter-implantation site on day 6 of pregnancy and on day 6 of pseudopregnancy. Our data suggest that the presence of an active blastocyst is required for mPGES-1 expression at the implantation site. When pregnant rats on day 5 were treated with nimesulide for 24 h, mPGES-1 protein expression was completely inhibited. cPGES immunostaining was clearly observed in the luminal epithelium and subluminal stromal cells immediately surrounding the implanting blastocyst on day 6 of pregnancy. mPGES-2 immunostaining was clearly seen in the luminal epithelium at the implantation site. Additionally, immunostaining for prostaglandin I synthase (PGIS) was also strongly detected at the implantation site. In conclusion, our results indicate that PGE2 and PGI2 should have a very important role in rat implantation.

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