Expression and regulation of lanosterol 14α-demethylase in mouse embryo and uterus during the peri-implantation period

2008 ◽  
Vol 20 (8) ◽  
pp. 964 ◽  
Author(s):  
Xiaoming Song ◽  
Ping Tai ◽  
Jun Yan ◽  
Baoshan Xu ◽  
Xiufen Chen ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Mouse Embryo ◽  
Cholesterol Biosynthesis ◽  
Embryo Implantation ◽  
Preimplantation Embryos ◽  
Oestrogen Treatment ◽  
Delayed Implantation ◽  
Progesterone Treatment ◽  
High Level ◽  
Implantation Period

Lanosterol 14α-demethylase (LDM) is expressed ubiquitously in all mammals and is important in cholesterol biosynthesis. However, whether LDM expression is involved in the interaction between uterus and embryo during implantation remains unknown. In the present study, the expression of LDM was investigated in mouse embryo and uterus during the peri-implantation period using confocal microscopy, immunohistochemistry and western blot methods. Further, regulation of LDM expression was investigated in pseudopregnancy, delayed implantation, artificial decidualisation and ovariectomisation using 17β-oestradiol and progesterone treatment mouse models. The results showed that LDM was selectively expressed in preimplantation embryos and the uterine subluminal stroma surrounding the implanting blastocyst on Day 5 of pregnancy. No corresponding signal was detected in the uterus on Day 5 of pseudopregnancy. Most notably, once delayed implantation was terminated by oestrogen treatment and the embryo implanted, a high level of LDM expression was induced in the subluminal stroma surrounding the implanting blastocyst, whereas no corresponding signal was detected in the delayed implantation uterus. A high level of LDM expression was observed in the uterus decidua on Days 6–8 of pregnancy. Furthermore, LDM expression was induced in the uterine stroma under artificial decidualisation. Oestrogen, but not progesterone, treatment induced a high level of LDM expression in the uterus of ovariectomised mice. These results indicate that LDM is closely related to mouse embryo implantation and can be upregulated by oestrogen.

scholarly journals Extracellular Ca2+-sensing receptor expression and hormonal regulation in rat uterus during the peri-implantation period

Reproduction ◽  
2005 ◽  
Vol 129 (6) ◽  
pp. 779-788 ◽  
Author(s):  
Li-Juan Xiao ◽  
Jin-Xiang Yuan ◽  
Yin-Chuan Li ◽  
Rui Wang ◽  
Zhao-Yuan Hu ◽  
...  
Keyword(s):  
Hormonal Regulation ◽  
Early Stage ◽  
Embryo Implantation ◽  
Receptor Expression ◽  
Luminal Epithelium ◽  
Rat Uterus ◽  
Delayed Implantation ◽  
Wide Range ◽  
High Level ◽  
Implantation Period

The extracellular Ca2+-sensing receptor (CaR) is a member of the superfamily of G protein-coupled receptors (GPCRs). It is an important mediator of a wide range of Ca2+-dependent physiological responses in various tissues. In reproductive tissues it has been reported to play a significant role in promoting or maintaining placentation. Meanwhile, another Ca2+regulated gene stanniocalcin-1 (STC-1) has been documented to be involved in decidualization and uterine remodelling. The phenomenon that CaR mediates STC-1’s transcription responding to extracellular calcium in fish urges us to suppose that CaR, like STC-1, may also play a role in implantation and decidualization. To resolve this conjecture, we have examined the expression and hormonal regulation of the CaR gene in rat uterus during peri-implantation period.CaR mRNA was expressed at a moderate level in the luminal epithelium of the early stage of pregnancy (from day 1 to day 3). From day 2–3 it began to be expressed more strongly in the stromal cells immediately underneath the luminal epithelium, but decreased to a basal level on day 4. From day 6 to day 9 continuously, both CaR mRNA and protein were highly expressed in the primary decidua. Expression of CaR mRNA and protein in these cells was also observed when a delayed implantation was terminated by estrogen treatment to allow the embryo implantation. In contrast, only basal level expression of the molecules was detected in the cells of animals subjected to a normal-delayed implantation or the pseudopregnant condition.Embryo transplantation experiment confirmed that CaR expression at the implantation site was induced by the implanting blastocyst. Consistent with the normal pregnant process, CaR mRNA and protein in the cells were also induced by an artificial decidualization procedure. Further experiments demonstrated that treatment of the ovariectomized rat with estrogen or/and progesterone stimulated a high level expression of CaR mRNA in the uterine epithelial and glandular epithelium. In conclusion, CaR was specifically induced during the processes of implantation and subsequent decidualization and may play a role in these processes.

Quantification of basigin mRNA in mouse oocytes and preimplantation embryos by competitive RT-PCR

Zygote ◽  
2002 ◽  
Vol 10 (3) ◽  
pp. 239-243 ◽  
Author(s):  
Nai-Zheng Ding ◽  
Cheng-Qiang He ◽  
Zeng-Ming Yang
Keyword(s):  
Embryo Implantation ◽  
Preimplantation Embryos ◽  
Immunoglobulin Superfamily ◽  
Competitive Polymerase Chain Reaction ◽  
Preimplantation Mouse Embryos ◽  
Preimplantation Mouse ◽  
Cell Embryo ◽  
Blastocyst Implantation ◽  
Polymerase Chain ◽  
High Level

Basigin is a member of the immunoglobulin superfamily and a key molecule related to mouse blastocyst implantation. Whether preimplantation mouse embryos express basigin mRNA is still unknown. The aim of this study was to use a quantitative competitive polymerase chain reaction to assess quantitatively the levels of basigin mRNA in mouse oocyte and preimplantation embryos. Basigin mRNA was detected in the oocyte and all the stages of preimplantation embryos. The levels of basigin mRNA were 0.0606 ± 0.0282 in the oocyte, 0.0102 ± 0.0036 in the zygote, 0.0007 ± 0.0003 in the 2-cell embryo, 0.0031 ± 0.0017 in the 4-cell embryo, 0.0084 ± 0.0024 in the 8-cell embryo, 0.0537 ± 0.0121 in the morula and 0.0392 ± 0.0161 attomoles in the blastocyst, respectively. The levels of basigin mRNA in the oocyte, morula and blastocyst were significantly higher than those in the zygote and embryos at the 2-cell, 4-cell and 8-cell stages. The high level of basigin expression in the blastocyst may play a role during embryo implantation.

scholarly journals Differential expression and regulation of Cryab in mouse uterus during preimplantation period

Reproduction ◽  
2013 ◽  
Vol 145 (6) ◽  
pp. 577-585 ◽  
Author(s):  
Xue-Chao Tian ◽  
Qu-Yuan Wang ◽  
Dang-Dang Li ◽  
Shou-Tang Wang ◽  
Zhan-Qing Yang ◽  
...  
Keyword(s):  
Real Time ◽  
Stromal Cells ◽  
Real Time Pcr ◽  
Embryo Implantation ◽  
Mouse Uterus ◽  
High Level ◽  
Implantation Period

The aim of this study was to examine the expression and regulation of the crystallin, alpha B (Cryab) gene in mouse uterus during the peri-implantation period by in situ hybridization and real-time PCR. There was no detectable Cryab mRNA signal on days 1–4 of pregnancy. On day 5 of pregnancy when embryo implanted, a high level of Cryab mRNA signal was found in the subluminal stroma surrounding the implanting blastocyst. On days 6–8, Cryab mRNA was strongly expressed in the primary decidua. By real-time PCR, a high level of Cryab expression was detected on days 7 and 8 of pregnancy, although Cryab expression was seen from days 1 to 8. Under in vivo and in vitro artificial decidualization, Cryab expression was significantly elevated. Compared with the progesterone-primed delayed implantation uterus, a high level of Cryab mRNA expression was observed in estrogen-activated implantation uterus. In the uterine stromal cells, cAMP, estrogen, and progesterone could induce the expression of Cryab gene. In the ovariectomized mouse uterus, estrogen could also induce the expression of Cryab while progesterone inhibited its expression. Our data suggest that Cryab may play an important role during mouse embryo implantation and decidualization and that estrogen and progesterone can regulate the expression of Cryab gene.

scholarly journals Differential expression of peroxisome proliferator-activated receptor delta at implantation sites and in decidual cells of rat uterus

Reproduction ◽  
2003 ◽  
pp. 817-825 ◽  
Author(s):  
NZ Ding ◽  
XH Ma ◽  
HL Diao ◽  
LB Xu ◽  
ZM Yang
Keyword(s):  
Embryo Implantation ◽  
Glandular Epithelium ◽  
Peroxisome Proliferator ◽  
Rat Uterus ◽  
Peroxisome Proliferator Activated Receptor ◽  
Oestrogen Treatment ◽  
Delayed Implantation ◽  
Decidual Cells ◽  
Implantation Sites

The aim of this study was to examine the expression and regulation of peroxisome proliferator-activated receptor delta (PPARdelta) gene in rat uterus during early pregnancy by in situ hybridization and immunohistochemistry. PPARdelta mRNA expression in the luminal epithelium was high on day 1 of pregnancy, gradually declined from day 2 and was undetectable on day 5 of pregnancy. However, expression in the glandular epithelium began to increase from day 2 and was high on day 5 of pregnancy. There was no detectable PPARdelta immunostaining in the luminal and glandular epithelium from day 1 to day 5. On day 6 of pregnancy when embryos implanted, PPARdelta mRNA and immunostaining were intense in the subluminal stroma at implantation sites. On days 7 and 8, there was strong expression of both PPARdelta mRNA and intense immunostaining in the decidualized area near the lumen. There was low expression of PPARdelta in the subluminal stroma and glandular epithelium under delayed implantation. After delayed implantation was terminated by oestrogen treatment and embryo implantation was initiated, both PPARdelta mRNA and immunostaining were strongly induced in the subluminal stroma. Intense PPARdelta immunostaining was observed in the decidua under artificial decidualization, while no detectable immunostaining was seen in the uninjected control horn. Retinoid X receptor (RXRalpha) immunostaining was seen in the subluminal stroma surrounding the implanting blastocyst on day 6 and in the decidual cells on days 7 and 8 of pregnancy. In conclusion, the high PPARdelta expression at implantation sites and in the decidual cells in rat uterus indicates that PPARdelta may play an important role during implantation and decidualization.

scholarly journals Basigin expression and hormonal regulation in the rat uterus during the peri-implantation period

Reproduction ◽  
2002 ◽  
pp. 219-225 ◽  
Author(s):  
LJ Xiao ◽  
HL Diao ◽  
XH Ma ◽  
NZ Ding ◽  
K Kadomatsu ◽  
...  
Keyword(s):  
Expression Pattern ◽  
Hormonal Regulation ◽  
Ovariectomized Rats ◽  
Luminal Epithelium ◽  
Rat Uterus ◽  
Oestrogen Treatment ◽  
Delayed Implantation ◽  
Similar Expression Pattern ◽  
Implantation Period ◽  
Basal Concentration

Basigin is essential for fertilization and implantation. The aim of this study was to determine the expression and hormonal regulation of the basigin gene in the rat uterus during the peri-implantation period. Basigin mRNA was localized strongly in the luminal epithelium on day 1 of pregnancy and gradually decreased to a basal concentration from day 3 to day 5 of pregnancy. Basigin mRNA and protein were expressed strongly in the implanting blastocyst and primary decidua on day 6 of pregnancy. A similar expression pattern was also induced in the uterus after delayed implantation was terminated by oestrogen treatment and the embryo implanted, whereas expression was not detected during delayed implantation. Basigin expression was not detected on day 6 of pseudopregnancy. Basigin mRNA was expressed strongly in the decidua on days 7 and 8 of pregnancy. Furthermore, both basigin mRNA and protein were induced in the decidua during artificial decidualization. In addition, oestrogen stimulated strong expression of basigin mRNA in the uterine epithelium of ovariectomized rats. These findings indicate that basigin may play a role during implantation and decidualization in rats.

scholarly journals MRI analysis of angiogenesis during mouse embryo implantation

2006 ◽  
Vol 55 (5) ◽  
pp. 1013-1022 ◽  
Author(s):  
Vicki Plaks ◽  
Vyacheslav Kalchenko ◽  
Nava Dekel ◽  
Michal Neeman
Keyword(s):  
Mouse Embryo ◽  
Embryo Implantation

Delayed implantation induced by letrozole in mice

2021 ◽  
Author(s):  
Fang Wang ◽  
Shijie Li ◽  
Lingshuai Meng ◽  
Ye Kuang ◽  
Zhonghua Liu ◽  
...  
Keyword(s):  
Embryo Implantation ◽  
Late Pregnancy ◽  
High Dose ◽  
Successful Pregnancy ◽  
Short Delay ◽  
Wild Mice ◽  
Plasma Progesterone ◽  
Delayed Implantation ◽  
Gene Ablation ◽  
At Will

Implantation timing is key for a successful pregnancy. Short delay in embryo implantation caused by targeted gene ablation produced a cascading problem in the later stages of the pregnancy. Although several delayed implantation models have been established in wild mice, almost none of them is suitable for investigating the delay on the late events of pregnancy. Here, we report a new delayed implantation model established by the intraperitoneally administration of letrozole at 5 mg/kg body weight on the day 3 of pregnancy. In these mice, initiation of implantation was induced at will by the injection of estradiol (E2). When the estradiol (3 ng) was injected on day 4 of pregnancy (i.e., without delay), the embryo implantation restarted, and the pregnancy continued normally. However, high dose of estrogen (25 ng) caused compromised implantation. We also found that only 67% of the female mice could be pregnant normally and finally gave birth when the injection of estradiol (3 ng) was on day 5 of pregnancy (i.e., one day delay). Most of the failed pregnancies had impaired decidualization, decreased plasma progesterone levels and compromised angiogenesis. Progesterone supplementation could rescue decidualization failure in the mice. Collectively, we established a new model of delayed implantation by letrozole, which can be easily used to study the effect and mechanisms of delay of embryo implantation on the progression of late pregnancy events.

2-Methoxyoestradiol impairs mouse embryo implantation via F-spondin

2019 ◽  
Vol 31 (4) ◽  
pp. 689
Author(s):  
Emanuel Guajardo-Correa ◽  
Denisse Mena-Silva ◽  
Patricia Diaz ◽  
Carlos Godoy-Guzmán ◽  
Hugo Cardenas ◽  
...  
Keyword(s):  
Mouse Embryo ◽  
Embryo Implantation ◽  
Target Protein ◽  
Methyl Transferase ◽  
Uterine Fluid ◽  
Protein F

The anti-implantation effects of high oestradiol (E2) concentrations could be mediated by E2 metabolites. Herein, we examined whether 2-methoxyoestradiol (2ME) impairs embryo implantation via its target protein F-spondin. Mice on Day 3 of pregnancy were treated with E2 concomitantly with the cathecol-O-methyl transferase inhibitor OR486 and the number of implanted embryos was recorded 5 days later. The effect of 2ME or 4-methoxyoestradiol (4ME) on embryo implantation was also investigated. Plasma and uterine levels of 2ME were measured 0.5, 1 or 3h after E2 treatment while the mRNA for spondin 1 (Spon1) and F-spondin were determined in the uterus 3, 6, 12 or 24h after 2ME treatment. Finally, the effect of a neutralising F-spondin antibody on the anti-implantation effect of 2ME was explored. OR486 blocked the anti-implantation effect of E2; 2ME, but not 4ME, affected embryo implantation. The 2ME concentration was increased after 0.5 and 1h in plasma and 3h in uterine fluid following E2 treatment. 2ME increased levels of Spon1 at 12 and 24h although F-spondin was increased at 12h. F-spondin antibody blocked the effect of 2ME on embryo implantation. We conclude that 2ME impairs mouse embryo implantation via activation of F-spondin in the uterus.

scholarly journals Immunolocalization of steroidogenic enzymes in the corpus luteum and placenta of the Japanese black bear, Ursus thibetanus japonicus, during pregnancy

Reproduction ◽  
2001 ◽  
pp. 587-594 ◽  
Author(s):  
T Tsubota ◽  
S Taki ◽  
K Nakayama ◽  
JI Mason ◽  
S Kominami ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Corpus Luteum ◽  
Fetal Development ◽  
Black Bear ◽  
Corpora Lutea ◽  
Luteal Cells ◽  
Ursus Thibetanus ◽  
Delayed Implantation ◽  
Post Implantation ◽  
Implantation Period

The Japanese black bear, Ursus thibetanus japonicus, is a seasonal breeder and shows delayed implantation for several months during pregnancy. The objective of this study was to clarify the steroidogenic capability of the corpus luteum and placenta during pregnancy, including both delayed implantation and fetal development, by immunolocalization of steroidogenic enzymes in these organs of the Japanese black bear. Ovaries and placentae from 15 wild Japanese black bears, which had been killed legally by hunters and were thought to be pregnant, were used in an immunocytochemical study to localize the cholesterol side chain cleavage cytochrome P450 (P450scc), 3beta-hydroxysteroid dehydrogenase (3betaHSD), 17alpha-hydroxylase cytochrome P450 (P450c17) and aromatase cytochrome P450 (P450arom) by the avidin-biotin-peroxidase complex method using polyclonal antisera raised in mammals against P450scc, 3betaHSD, P450c17 and P450arom. P450scc and 3betaHSD were localized in all luteal cells throughout pregnancy. P450c17 was present in a few luteal cells, especially in the outer area of the corpus luteum throughout pregnancy, but the number of positively immunostained cells decreased during the post-implantation period. Cells positively immunostained for P450c17 were significantly smaller than negatively immunostained cells (P < 0.01). P450arom was present sporadically in a few luteal cells throughout pregnancy, but the number of positively immunostained cells decreased during the post-implantation period. The size of cells positively immunostained for P450arom was not significantly different from that of negatively immunostained cells. The whole placenta was negatively immunostained for P450scc, 3betaHSD and P450c17, but P450arom was present in the syncytiotrophoblasts and endothelial cells of maternal blood vessels. These results indicate that, in the Japanese black bear, corpora lutea are a source of progesterone which may play an important role in the maintenance of delayed implantation and fetal development during pregnancy. Corpora lutea have a minimum capability to synthesize androgen in small luteal cells and oestrogen in normal-sized luteal cells during pregnancy, and placentae have the ability to synthesize oestrogen during late pregnancy.

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