scholarly journals Local changes in blood flow within the preovulatory follicle wall and early corpus luteum in cows

Reproduction ◽  
2003 ◽  
pp. 759-767 ◽  
Author(s):  
TJ Acosta ◽  
KG Hayashi ◽  
M Ohtani ◽  
A Miyamoto
Keyword(s):  
Blood Flow ◽  
Corpus Luteum ◽  
Plasma Concentrations ◽  
Maximum Velocity ◽  
Initial Increase ◽  
Preovulatory Follicle ◽  
Lh Surge ◽  
Functional Changes ◽  
Induced Ovulation ◽  
Doppler Image

Haemodynamic changes are involved in the cyclic remodelling of ovarian tissue that occurs during final follicular growth, ovulation and new corpus luteum development. The aim of this study was to characterize the real-time changes in the blood flow within the follicle wall associated with the LH surge, ovulation and corpus luteum development in cows. Normally cyclic cows with a spontaneous ovulation (n = 5) or a GnRH-induced ovulation (n = 5) were examined by transrectal colour and pulsed Doppler ultrasonography to determine the area and the time-averaged maximum velocity (TAMXV) of the blood flow within the preovulatory follicle wall and the early corpus luteum. Ultrasonographic examinations began 48 h after a luteolytic injection of PGF(2alpha) analogue was given at the mid-luteal phase of the oestrous cycle. Cows with spontaneous ovulation were scanned at 6 h intervals until ovulation occurred. Cows with GnRH-induced ovulation were scanned just before GnRH injection (0 h), thereafter at 0.5, 1, 2, 6, 12, 24 h and at 24 h intervals up to day 5. Blood samples were collected at the same time points for oestradiol, LH and progesterone determinations. Cows with both spontaneous and GnRH-induced ovulation showed a clear increase in the plasma concentration of LH (LH surge) followed by ovulation 26-34 h later. In the colour Doppler image of the preovulatory follicle, the blood flow before the LH surge was detectable only in a small area in the base of the follicle. An acute increase in the blood flow velocity (TAMXV) was detected at 0.5 h after GnRH injection, synchronously with the initiation of the LH surge. At 12 h after the LH surge, the plasma concentrations of oestradiol decreased to basal concentrations. The TAMXV remained unchanged after the initial increase until ovulation, but decreased on day 2 (12-24 h after ovulation). In the early corpus luteum, the blood flow (area and TAMXV) gradually increased in parallel with the increase in corpus luteum volume and plasma progesterone concentration from day 2 to day 5, indicating active angiogenesis and normal luteal development. Collectively, the complex structural, secretory and functional changes that take place in the ovary before ovulation are closely associated with a local increase in the blood flow within the preovulatory follicle wall. The result of the present study provides the first visual information on vascular and blood flow changes associated with ovulation and early corpus luteum development in cows. This information may be essential for future studies involving pharmacological control of blood flow and alteration of ovarian function.

Temporal relationships of the LH surge and ovulation to echotexture and power Doppler signals of blood flow in the wall of the preovulatory follicle in heifers

2010 ◽  
Vol 22 (7) ◽  
pp. 1110 ◽  
Author(s):  
M. A. R. Siddiqui ◽  
J. C. Ferreira ◽  
E. L. Gastal ◽  
M. A. Beg ◽  
D. A. Cooper ◽  
...  
Keyword(s):  
Blood Flow ◽  
Power Doppler ◽  
Initial Increase ◽  
Preovulatory Follicle ◽  
Lh Surge ◽  
Follicle Diameter ◽  
Temporal Relationships ◽  
Preovulatory Follicles ◽  
Doppler Signals ◽  
Gonadotrophin Releasing Hormone

Changes in echotexture and blood flow in the wall of preovulatory follicles in heifers were studied in relation to the LH surge and ovulation in gonadotrophin-releasing hormone-induced (n = 7; Experiment 1) and spontaneous (n = 8; Experiment 2) ovulators. Ultrasonographic examinations and blood sampling were performed either every hour (Experiment 1) or every 6 h (Experiment 2). The interval from LH peak to ovulation in induced and spontaneous ovulators was 27.1 ± 0.3 and 34.5 ± 1.5 h, respectively. Follicle diameter did not increase between the LH peak and ovulation. In the induced ovulators, serration of the stratum granulosum was detected in one (14%), two (29%), three (43%) and four (57%) heifers at 4, 3, 2 and 1 h before ovulation, respectively. An initial increase in blood flow (P < 0.001) encompassed the LH peak in both experiments. In the induced ovulators, blood flow increased (P < 0.02) to maximum 3 h after the LH peak, maintained a plateau for 5 h, decreased (P < 0.05) between 9 and 14 h, increased (P < 0.05) again between 19 and 21 h and then decreased (P < 0.01) between 25 and 26 h (1 h before ovulation). The biphasic increase and decrease in blood flow and serration of the granulosum in the wall of the preovulatory follicle in cattle are novel findings.

EVIDENCE THAT CHANGES IN LUTEINIZING HORMONE SECRETION REGULATE THE GROWTH OF THE PREOVULATORY FOLLICLE IN THE EWE

1981 ◽  
Vol 90 (3) ◽  
pp. 375-389 ◽  
Author(s):  
K. P. McNATTY ◽  
MARION GIBB ◽  
CAROLYN DOBSON ◽  
D. C. THURLEY
Keyword(s):  
Luteinizing Hormone ◽  
Corpus Luteum ◽  
Plasma Levels ◽  
Important Determinant ◽  
Hormone Secretion ◽  
Preovulatory Follicle ◽  
Luteinizing Hormone Secretion ◽  
Lh Surge ◽  
Follicular Maturation ◽  
Lh Rh

The aim of the present study was to gain evidence that the level of LH secretion preceding the preovulatory LH surge is an important determinant of follicular maturation and corpus luteum function in the ewe. In addition it was hoped to establish whether the pattern of LH delivery to the ovary (pulsatile v. constant) is a critical factor in the maturation of a preovulatory follicle. To accomplish this, progesterone-primed anoestrous ewes were repeatedly injected i.v. with LH or luteinizing hormone-releasing hormone (LH-RH), or given an i.v. infusion of LH, over a 72 h period. These animals, together with the appropriate controls, were exposed to a sexually active ram so that oestrous activity could be recorded. All ewes were subjected to intensive blood sampling regimes so that the plasma levels of LH and progesterone could be determined and compared to those which occurred in the same breed of sheep during the oestrous cycle. Collectively the data suggest that the plasma levels of LH preceding the preovulatory LH surge are an important determinant of follicular maturation as judged by subsequent corpus luteum function. Moreover, they show that follicular maturation can be achieved with widely differing patterns of LH delivery to the ovary during the preovulatory period and that a strict pulsatile delivery of LH may not be an absolute requirement.

176 BLOOD FLOW TO THE CORPUS LUTEUM AND PREOVULATORY FOLLICLE AFTER OVULATION INDUCTION DURING FIRST VERSUS SECOND WAVE IN WATER BUFFALO

2015 ◽  
Vol 27 (1) ◽  
pp. 179
Author(s):  
S. Caunce ◽  
D. Dadarwal ◽  
P. S. Brar ◽  
J. Singh
Keyword(s):  
Blood Flow ◽  
Corpus Luteum ◽  
Prostaglandin F2α ◽  
Preovulatory Follicle ◽  
Dominant Follicle ◽  
Flow Area ◽  
Vascular Area ◽  
Preovulatory Follicles ◽  
Luteal Tissue ◽  
Second Wave

The objective of the study was to compare the blood flow to the corpus luteum (CL) and the preovulatory follicle in dairy buffalo (Bubalus bubalis) when ovulation was induced during the first (low to increasing progesterone levels) versus the second (luteal progesterone levels) follicular wave. We hypothesised that the wall of the first-wave dominant follicle will be less vascular compared with that of the second-wave follicle. The study was conducted during the summer months in Punjab, India. Ovulation was synchronized with prostaglandin F2α (PGF) IM followed by gonadotropin-releasing hormone (GnRH) IM 48 h later (Day 0) and buffaloes were randomised to first wave (FW; n = 6) and second wave (SW; n = 7) groups. FW group was given PGF on Days 6.5 and 7, and GnRH on Day 9.5 followed by AI (14–16 h after GnRH). The SW group was given GnRH on Day 7 (to induce ovulation of first-wave dominant follicle without luteolysis and synchronous emergence of next wave), PGF on Days 13.5 and 14, GnRH on Day 16.5 followed by artificial insemination. Transrectal colour Doppler ultrasonography (MyLab5 Vetwith 7.5 MHz transducer, Esaote S.p.A, Genoa, Italy) was performed daily and 20-s cineloops of each ovary were recorded under standardized gain controls. Images from the cineloops were processed using Fiji (ImageJ, National Institutes of Health, Bethesda, MD, USA) to calculate the area of blood flow (coloured area = vascular area, grey scale area = tissue area, and their ratio) for the preovulatory follicle (on the day before ovulation) and luteal tissue (on the day of PGF injection and 4 days post-ovulation). Data were analysed by t-test from the animals that ovulated one day before (n = 3) or the day of AI (n = 6) and had a functional CL at day 5 post-AI (FW n = 5, SW n = 4). FW follicles ovulated on 8.6 ± 0.3 days from wave emergence compared with SW follicles on 10.0 ± 0.6 days (P < 0.05) but were similar in size (i.e. follicular area on the day before ovulation did not differ between groups; P = 0.5). There was no difference in the blood flow area in the wall of preovulatory follicles (P = 0.4). Vascular area of follicles was strongly correlated with their diameter (r = 0.87). Follicles >13.5 mm in diameter had more blood flow in their wall than smaller follicles (P < 0.01). FW had a tendency (P = 0.07) for smaller luteal area on the day of PGF treatment (FW = 171 ± 24 mm2; SW = 332 ± 81 mm2) and tended (P = 0.06) to have less vascular area in the CL compared to SW group (FW = 30 ± 6 mm2; SW = 67 ± 17 mm2). There was no difference (P = 0.5) between the groups for vascular to CL area ratio. The area of luteal tissue and blood flow to the CL at Day 4 post-ovulation did not differ between the groups (P = 0.4). The diameter of the preovulatory follicle (11.6–15.7 mm) was not correlated with the cross-sectional area of developing CL at Day 4 post-ovulation (r = 0.09). In conclusion, vascularity to preovulatory follicles originating from the first wave v. second wave did not differ and preovulatory follicles ≥13.5 mm were more vascular than smaller follicles. Research was funded by NSERC; the first author was funded by scholarships from WCVM and GADVASU.

Follicle vascularity coordinates corpus luteum blood flow and progesterone production

2017 ◽  
Vol 29 (3) ◽  
pp. 448 ◽  
Author(s):  
S. G. S. de Tarso ◽  
G. D. A. Gastal ◽  
S. T. Bashir ◽  
M. O. Gastal ◽  
G. A. Apgar ◽  
...  
Keyword(s):  
Blood Flow ◽  
Corpus Luteum ◽  
Doppler Ultrasonography ◽  
Beef Cows ◽  
Regression Analyses ◽  
Preovulatory Follicle ◽  
Colour Doppler ◽  
Plasma Progesterone ◽  
Colour Doppler Ultrasonography ◽  
Progesterone Production

Colour Doppler ultrasonography was used to compare the ability of preovulatory follicle (POF) blood flow and its dimensions to predict the size, blood flow and progesterone production capability of the subsequent corpus luteum (CL). Cows (n = 30) were submitted to a synchronisation protocol. Follicles ≥7 mm were measured and follicular wall blood flow evaluated every 12 h for approximately 3.5 days until ovulation. After ovulation, cows were scanned daily for 8 days and similar parameters were evaluated for the CL. Blood samples were collected and plasma progesterone concentrations quantified. All parameters were positively correlated. Correlation values ranged from 0.26 to 0.74 on data normalised to ovulation and from 0.31 to 0.74 on data normalised to maximum values. Correlations between calculated ratios of both POF and CL in data normalised to ovulation and to maximum values ranged from moderate (0.57) to strong (0.87). Significant (P < 0.0001) linear regression analyses were seen in all comparisons. In conclusion, higher correlations were observed between the dimensions of POF and/or CL and blood flow of both structures, as well as POF and/or CL blood flow with plasma progesterone concentrations of the resultant CL. These findings indicate that follicle vascularity coordinates CL blood flow and progesterone production in synchronised beef cows.

116 THE CHARACTERISTICS OF CORPUS LUTEUM SIZE, BLOOD FLOW, AND PLASMA PROGESTERONE CONCENTRATION AFTER OVULATION OF THE FIRST AND SECOND WAVE DOMINANT FOLLICLE

2014 ◽  
Vol 26 (1) ◽  
pp. 172
Author(s):  
R. Miura ◽  
H. Takahashi ◽  
S. Haneda ◽  
M. Matsui
Keyword(s):  
Blood Flow ◽  
Corpus Luteum ◽  
Cross Sectional Area ◽  
Sectional Area ◽  
Preovulatory Follicle ◽  
Dominant Follicle ◽  
Flow Area ◽  
Cross Sectional ◽  
Follicular Wave ◽  
And Function

The characteristics of the corpus luteum (CL) are greatly affected by the characteristics of the preceeding preovulatory follicle, such as follicle size and function. A previous study reported that the concentration of oestradiol in follicular fluid and production of androstenedione and progesterone (P4) by cultured theca cells are higher in the first follicular wave dominant follicle than in the second follicular wave dominant follicle. In addition, blood flow in the wall of the preovulatory follicle is higher in the first follicular wave than in the second follicular wave. These results suggest that the characteristics of the dominant follicle in the first and the second follicular wave may be different. The objective of this study was to compare CL characteristics, such as diameter, cross-sectional area, blood flow area, and plasma P4 concentration after ovulation of the dominant follicle between the first and the second follicular waves. Preovulatory follicles and CL formed after ovulation of the first follicular wave (W1; n = 5) and second follicular wave (W2; n = 6) were examined in non-lactating Holstein cows. In W1, PGF2α and GnRH were administrated on D7 and D9 of the oestrus cycle (D0 = oestrus), respectively, to induce follicular maturation. In W2, GnRH was administrated on D6 to induce a new follicular wave; subsequently, PGF2α and GnRH were administrated on D14 and D16, respectively. Diameter and percentage of follicular circumference with blood flow of preovulatory follicle on D10 in W1 and D17 in W2 were measured by transrectal colour Doppler ultrasonography. Diameter, cross-sectional area, and blood flow area of CL formed after ovulation in W1 (W1CL) and W2 (W2CL) were also examined on Day 3, 6, and 9 after ovulation (Day 1 = ovulation day). Blood samples were collected from Day 1 to 9 for P4 measurement. Quantitative end-points for diameter and percentage of follicular circumference with blood flow of the preovulatory follicle were analysed between the groups by using the unpaired Student's t-test. Diameter, cross-sectional area, blood flow area of CL, and plasma P4 concentration were analysed by repeated-measures ANOVA followed by Scheffe's F-test as a multiple comparison test. Larger diameter and higher percentage of follicular circumference with blood flow of the preovulatory follicle were observed in W1 compared with those in W2 (P < 0.01). Diameter of CL was larger in W1CL than in W2CL regardless of day (P < 0.001). Cross-sectional area of CL was larger in W1CL than in W2CL on Day 6 and 9 but not on Day 3. Blood flow area of CL was larger in W1CL than in W2CL on Day 3 and 6 but not in Day 9. Plasma P4 concentrations were higher in W1CL than in W2CL on Day5 and 7 (P < 0.05). In conclusion, larger size and higher percentage of follicular circumference with blood flow of preovulatory follicle in W1 lead to a larger size and a blood flow area of CL as well as higher plasma P4 concentration. These results suggest that preovulatory blood flow status affects the morphology and function of CL.

An in vivo Model for the Assessment of Acute Fibrinolytic Capacity of the Endothelium

1997 ◽  
Vol 78 (04) ◽  
pp. 1242-1248 ◽  
Author(s):  
David E Newby ◽  
Robert A Wright ◽  
Christopher A Ludlam ◽  
Keith A A Fox ◽  
Nicholas A Boon ◽  
...  
Keyword(s):  
Blood Flow ◽  
Substance P ◽  
Sodium Nitroprusside ◽  
Plasma Concentrations ◽  
In Vivo Model ◽  
Forearm Circulation ◽  
Von Willebrand ◽  
Local Release ◽  
Willebrand Factor

SummaryThe effects on blood flow and plasma fibrinolytic and coagulation parameters of intraarterial substance P, an endothelium dependent vasodilator, and sodium nitroprusside, a control endothelium independent vasodilator, were studied in the human forearm circulation. At subsystemic locally active doses, both substance P (2-8 pmol/min) and sodium nitroprusside (2-8 μg/min) caused dose-dependent vasodilatation (p <0.001 for both) without affecting plasma concentrations of PAI-1, von Willebrand factor antigen or factor VIII:C activity. Substance P caused local increases in t-PA antigen and activity (p <0.001) in the infused arm while sodium nitroprusside did not. At higher doses, substance P increased blood flow and t-PA concentrations in the noninfused arm. We conclude that brief, locally active and subsystemic infusions of intraarterial substance P cause a rapid and substantial local release of t-PA which appear to act via a flow and nitric oxide independent mechanism. This model should provide a useful and selective method of assessing the in vivo capacity of the forearm endothelium to release t-PA acutely.

Corpus luteum blood flow in abnormal early pregnancy.

1996 ◽  
Vol 15 (9) ◽  
pp. 645-649 ◽  
Author(s):  
J L Alcázar ◽  
C Laparte ◽  
G López-Garcia
Keyword(s):  
Blood Flow ◽  
Corpus Luteum ◽  
Early Pregnancy
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