scholarly journals Measurement of serum and peritoneal fluid LH concentrations as a diagnostic tool for human endometriosis

Reproduction ◽  
2001 ◽  
pp. 761-769 ◽  
Author(s):  
JC Illera ◽  
G Silvan ◽  
MJ Illera ◽  
CJ Munro ◽  
BA Lessey ◽  
...  
Keyword(s):  
Predictive Value ◽  
Peritoneal Fluid ◽  
Polyclonal Antiserum ◽  
Control Group ◽  
Standard Curve ◽  
Serum Lh ◽  
Assay Time ◽  
Cycle Dependent ◽  
And Control ◽  
One Way Anova

A rapid, sensitive enzymeimmunoassay for the measurement of LH concentrations in serum and peritoneal fluid samples of healthy women and women with endometriosis is reported. The ligand (LH) was captured by a readily available, widely used and well-characterized monoclonal antibody (mAb, 518B7) generated against the beta subunit of bovine LH. This mAb, although specific for LH, shows very little species specificity and detects LH by radioimmunoassay in humans. A polyclonal antiserum raised in rabbits against hCG was conjugated to horseradish peroxidase and was used as the second antibody signal. This anti-hCG antiserum crossreacts with LH. The enzymeimmunoassay uses the standard human LH (hLH) preparations (NIADDK-hLH-I-3, AFP-827OB) and results are based on the relative concentrations of LH in serum and peritoneal fluid. Total assay time was < 3 h. The range of the standard curve was 0.002-0.500 ng LH per well and the lowest concentration of hLH that could be distinguished from zero concentration was 0.15 +/- 0.02 ng ml(-1) serum and 0.058 +/- 0.021 ng ml(-1) peritoneal fluid. Clinical diagnostic parameters for the LH enzymeimmunoassay showed a sensitivity of 85.71%, specificity 92.50%, efficiency 88.54%, positive predictive value 94.11% and negative predictive value 82.22%. The study was retrospective. Serum LH concentrations of women with endometriosis were 13.67 +/- 7.21 ng ml(-1), whereas serum LH concentrations of women in the control group were 4.52 +/- 2.03 ng ml(-1). One-way ANOVA showed significant differences (P < 0.001) between women with endometriosis and control groups. Women in the control group had peritoneal fluid LH values of 5.65 +/- 2.43 ng ml(-1), whereas peritoneal fluid LH values of 64.06 +/- 16.44 ng ml(-1) were obtained in women with endometriosis (P < 0.001). A cycle-dependent pattern of serum and peritoneal fluid LH concentration was observed in women in the control group, which was not observed in the peritoneal fluid of the group with endometriosis. The application of this assay to serum or peritoneal fluid samples provides the attractive possibility that it could be included in the panel of markers used for diagnosis of endometriosis.

Hyaluronan Preserves Peritoneal Membrane Transport Properties

2001 ◽  
Vol 21 (2) ◽  
pp. 136-143 ◽  
Author(s):  
Qun-Ying Guo ◽  
Wen-Xing Peng ◽  
Hui-Hong Cheng ◽  
Ren-Gao Ye ◽  
Bengt Lindholm ◽  
...  
Keyword(s):  
Membrane Transport ◽  
Peritoneal Fluid ◽  
Absorption Rate ◽  
Control Group ◽  
Fluid Absorption ◽  
Peritoneal Membrane ◽  
Lymphatic Absorption ◽  
Dialysis Solution ◽  
Significant Difference ◽  
And Control

Background We have shown that intraperitoneal (IP) addition of hyaluronan (HA) in a single dwell study in rat could increase peritoneal fluid removal by decreasing the peritoneal fluid absorption rate. In this study, we investigated the impact of repeated use of HA on peritoneal membrane transport characteristics. Methods Twelve male Sprague–Dawley rats received a once-daily IP injection of 25 mL 4.25% glucose dialysis solution without (HP group, n = 6) or with 0.025% HA (HA group, n = 6) for 1 week. Forty-eight hours after the last injection, a 4 hour dwell using 25 mL 4.25% glucose dialysis solution with IP volume marker and frequent dialysate and blood samplings was performed in each rat as well as in rats that did not receive any injection (control group, n = 8). Results Although the IP volumes were significantly lower in the HP and HA groups compared to the control group, IP volume in the HA group was significantly higher than in the HP group. Net ultrafiltration at 4 hours was 5.6 ± 1.3 mL, 10.2 ± 1.8 mL, and 13.2 ± 0.6 mL for the H P, HA, and control group, respectively. The peritoneal fluid absorption rate decreased by 45% in the HA group compared to the HP group. There was no significant difference in peritoneal fluid absorption rate between the HA and the control group. No difference was found in the direct lymphatic absorption rate between the HP and HA groups [0.010 ± 0.003 mL/minute in the HP group and 0.011 ± 0.004 mL/min in the HA group] although they were both higher than that of the control group (0.004 ± 0.001 mL/min). The solute transport rates were in general significantly higher in the HP group compared to the HA and control groups, and there was no significant difference between the latter two groups, except that protein transport rate was significantly lower in the HA group compared to the control group. Conclusions The present study suggests that ( 1 ) repeated exposure to hypertonic glucose-based dialysis solution results in increased peritoneal solute transport rates, as well as increased peritoneal fluid absorption rates; and ( 2 ) these changes, reflecting a highly permeable peritoneal membrane, were ameliorated by repeated IP addition of hyaluronan. The similar changes in the direct lymphatic absorption rate in rats that received daily IP injection of dialysis solution suggest that direct peritoneal lymphatic absorption was not influenced by hyaluronan.

scholarly journals Right ventricular free-wall longitudinal speckle tracking strain as a prognostic criterion of adverse outcomes in patients with pulmonary hypertension: a systematic review and meta-analysis

2021 ◽  
Vol 26 (4) ◽  
pp. 4417
Author(s):  
E. Z. Golukhova ◽  
I. V. Slivneva ◽  
M. L. Mamalyga ◽  
D. I. Marapov ◽  
M. N. Alekhin ◽  
...  
Keyword(s):  
Right Ventricular ◽  
Predictive Value ◽  
Speckle Tracking ◽  
Meta Analysis ◽  
Composite Endpoint ◽  
Adverse Outcomes ◽  
Control Group ◽  
Right Ventricular Free Wall ◽  
The Mean ◽  
And Control

The absence of consensus regarding the reference values of right ventricular free wall longitudinal strain (RVFWLS) and its predictive value prompted us to conduct a systematic review and meta-analysis of publications on the predictive role of this parameter in patients with pulmonary hypertension (PH).Aim. To study the independent predictive value of RVFWLS in PH patients using 2D/3D speckle tracking echocardiography.Material and methods. Firstly, 317 publications (PubMed) and 857 Google Scholar results were selected. Of the initially identified search results, 12 articles were analyzed. The papers were cohort designed.Results. The total number of patients with PH was 1281. The mean age of patients was 54,7±6,8 years. Four studies compared the RVFWLS with a control group (n=251). The mean RVFWLS were -17,0±2,4% and -24,7±2,2% in the experimental and control groups, respectively. A meta-analysis of the difference between the mean RVFWLS values in experimental and control group patients showed its total increase in PH subjects of 8,06% (95% CI: 5,18-10,94%; p<0,00001).The total number of deaths was 268 (all-cause — 180, composite endpoint — 88). According to the meta-analysis, with an increase of 1% in RVFWLS, there is an increase in mean all-cause mortality risk by 14% (p<0,00001), as well as mean risk of adverse outcomes or PH-related events (composite endpoint) by 14% (p<0,0001).Conclusion. These results highlight the high independent predictive value of RVFWLS as a predictor of adverse outcomes or events associated with a right ventricular dysfunction progression in PH patients.

scholarly journals Crossfit, musculação e corrida: vício, lesões e vulnerabilidade imunológica

2017 ◽  
Vol 86 (1) ◽  
Author(s):  
Thiago Guimarães ◽  
Marcos Carvalho ◽  
William Santos ◽  
Ercole Rubini ◽  
Wagner Coelho
Keyword(s):  
Physical Inactivity ◽  
Statistical Difference ◽  
Weight Training ◽  
Musculoskeletal Injuries ◽  
Exercise Dependence ◽  
Control Group ◽  
Weight Trainers ◽  
Total Dependence ◽  
And Control ◽  
One Way Anova

Introdução: A inatividade física figura como um dos principais fatores associados à mortalidade. Por outro lado, os possíveis prejuízos provocados pelo excesso de exercícios físicos não devem ser negligenciados.Objetivo: Avaliar e comparar o nível de dependência ao exercício entre diferentes modalidades, estimar a prevalência de lesões musculoesqueléticas e a vulnerabilidade imunológica em jovens assintomáticos.Métodos: Estudo observacional. Uma amostra com 219 pessoas foi dividida em cinco grupos: sedentários (e/ou insuficientemente ativos) (n=50), controle (moderadamente ativos) (n=31), praticantes de crossfit superativos (n=45), praticantes de musculação superativos (n=47) e corredores superativos (n=46). Além de uma anamnese para caracterizar a amostra e inferir o número de lesões musculoesqueléticas, gripes e infecções nos seis meses anteriores à coleta de dados, foi utilizada a Escala de Dependência de Exercício. Utilizou-se a ANOVA one way para analisar as diferenças estatísticas e para comparar os níveis de dependência total ao exercício, e a prevalência de lesões musculoesqueléticas, gripes e infecções.Resultados: O grupo musculação apresentou os maiores níveis de dependência ao exercício quando comparado com os grupos sedentários e controle (p<0,05). Quanto às lesões musculoesqueléticas, o grupo crossfit apresentou a maior prevalência, com diferença estatística em relação ao grupo controle. Gripes e infecções foram mais prevalentes nos grupos sedentários e musculação.Conclusão: Pessoas moderadamente ativas apresentaram menos lesões musculoesqueléticas e menor vulnerabilidade imunológica em relação a sedentários e superativos. A inatividade física e o vício em exercício parecem tornar o sistema imunológico mais vulnerável. Pessoas com hábitos extremos em relação à prática de atividades físicas (sedentários e adeptos superativos) podem ter a saúde comprometida.Crossfit, Weight Training and Running: Addiction, Injuries and Immunological VulnerabilityIntroduction: Physical inactivity is one of the main causes related to mortality. On the other hand, the possible damages caused by excessive exercise should not be neglected.Objective: To analyze the level of exercise dependence between different modalities, the prevalence of musculoskeletal injuries and immunological vulnerability in asymptomatic young people.Methods: This was an observational study. A sample of 219 people was divided into five groups: sedentary or insufficiently active (n=50), moderately active control (n=31), super active crossfitters (n=45), super active amateur weight trainers (n=47) and super active amateur runners (n=46). Additionally, an anamnesis to characterize the sample and to infer the number of musculoskeletal injuries, flus and infections in the six months prior to data collection, the Exercise Dependency Scale was used.  One way ANOVA was used to analyze the statistical differences and to compare the levels of total dependence to exercise, prevalence of musculoskeletal injuries, flu and infections.Results: The amateur weight trainers group presented higher levels of exercise dependence, with statistical difference in relation to the sedentary and control groups. Regarding the musculoskeletal lesions, the crossfit group presented the highest occurrence, with a statistical difference in relation to the control group. Flus and infections were more prevalent in sedentary and weight training groups.Conclusion: Moderately active people have fewer musculoskeletal injuries and less immunological vulnerability to sedentary and overactive ones. Physical inactivity and exercise addiction appear to make the immune system more vulnerable... People with extreme habits in relation to the practice of physical activities (sedentary and super active adepts) can have their health compromised.

scholarly journals Impact of Meningococcal B (4CMenB) Vaccine on Pharyngeal Neisseria meningitidis Carriage Density and Persistence in Adolescents

2020 ◽  
Author(s):  
Mark McMillan ◽  
Luke Walters ◽  
Thomas Sullivan ◽  
Lex E X Leong ◽  
Mark Turra ◽  
...  
Keyword(s):  
Neisseria Meningitidis ◽  
Quantitative Polymerase Chain Reaction ◽  
Cluster Randomized Trial ◽  
Control Group ◽  
Population Exposure ◽  
Standard Curve ◽  
Cluster Randomized ◽  
4Cmenb Vaccine ◽  
And Control ◽  
The Impact

Abstract Background Higher density of Neisseria meningitidis carriage may be associated with transmission of the meningococcus. Our aim was to establish the impact of meningococcal B (4CMenB) vaccine on N. meningitidis carriage density. Methods We compared 4CMenB vaccine to control among 913 South Australian students aged approximately 15–18 years in a cluster randomized trial who had N. meningitidis carriage at 12 months. Oropharyngeal swabs were collected at baseline and 12 months later to detect N. meningitidis carriage. Colony-forming units per milliliter (CFU/mL) were estimated by generating a standard curve that plotted quantitative polymerase chain reaction cycle threshold values against log-normalized CFU. Results Among the 913 students with N. meningitidis carriage at 12 months, there was no difference in mean carriage density between the vaccinated (n = 434; 3.80 log CFU/mL [standard deviation {SD}, 1.29]) and control group (n = 479; 3.73 log CFU/mL [SD, 1.30]; P = .51). Higher N. meningitidis carriage density at baseline was associated with an increase in the odds of persistent carriage at 12 months (n = 504; odds ratio [OR] per 1.0 log CFU/mL increase in density, 1.36 [95% confidence interval {CI}, 1.17–1.58]; P &lt; .001). Students with baseline carriage who were vaccinated had decreased persistent N. meningitidis carriage at 12 months compared to unvaccinated students (81/260 [31%] vs 105/244 [43%]; OR, 0.60 [95% CI, .40–.90]; P = .01). Conclusions 4CMenB vaccine did not reduce carriage density of N. meningitidis 12 months postvaccination, despite increased carriage clearance. Higher carriage density is likely to enable transmission through prolonged periods of population exposure. Clinical Trials Registration NCT03089086.

scholarly journals Efecto del entrenamiento en ábaco mental sobre la flexibilidad cognitiva: un estudio exploratorio

2020 ◽  
Vol 18 (3) ◽  
pp. 316-323
Author(s):  
Daniela Giorgetti ◽  
Emilio López-Navarro ◽  
Enric Munar
Keyword(s):  
Cognitive Flexibility ◽  
Mental Arithmetic ◽  
Control Group ◽  
Promising Tool ◽  
Trail Making ◽  
And Control ◽  
Arithmetic Instruction ◽  
Math Problems ◽  
Academic Year ◽  
One Way Anova

Mental Abacus (MA) training teaches students to solve math problems by visualizing a physical abacus structure to perform arithmetic operations. Research shows that MA practice relates with enhanced working memory in children, but other cognitive processes that could mediate the benefits registered remain unknown. The aim of the study was to analyze the effect of MA training in a cognitive flexibility task in twelve-year-old children, and compare it with a control group. 121 children from the sixth course of primary education were recruited. 54 students received MA training added during the academic year, while the control group received normative arithmetic instruction. MA training was provided by UCMAS Mental Arithmetic Spain S.L. To assess cognitive flexibility, we used the Trail Making Test (TMT). Data analysis entailed parametric assumptions check and a one-way ANOVA between MA and control group. There were no differences between groups in age. There were statistical differences in TMT-A (Z=-5,78, p<,001, d=,67) and TMT-B scores (Z=-2,24, p=,021, d=,08). Our data suggest that MA enhances cognitive flexibility in children. MA is a promising tool teaching math which benefits go beyond arithmetic calculation.

scholarly journals PENGARUH PELATIHAN SIDE JUMP SPRINT DAN SCISSOR JUMP TERHADAP DAYA LEDAK OTOT TUNGKAI PADA SISWA PUTRA PESERTA EKSTRAKURIKULER BOLAVOLI SMP NEGERI 3 ABIANSEMAL TAHUN PELAJARAN 2016/2017

2018 ◽  
Vol 6 (2) ◽  
Author(s):  
I Gede Sunarba . ◽  
Dr. I Ketut Sudiana, S.Pd. M Kes . ◽  
Gede Doddy Tisna, M.S., S.Or., M.Or. .
Keyword(s):  
Treatment Group ◽  
Vertical Jump ◽  
Control Group ◽  
Limb Muscle ◽  
Sprint Training ◽  
Jump Training ◽  
Explosive Power ◽  
Significant Difference ◽  
And Control ◽  
One Way Anova

Penelitian ini bertujuan untuk mengetahui pengaruh pelatihan side jump sprint dan scissor jump terhadap peningkatan daya ledak otot tungkai. Jenis penelitian ini adalah eksperimen semu dengan rancangan the non-randomized control group pretest posttest design. Subjek penelitian adalah siswa putra peserta ektrakurikuler bolavoli SMP Negeri 3 Abiansemal tahun pelajaran 2016/2017 berjumlah 45 orang, kemudian dibagi menjadi 3 kelompok dengan menggunakan teknik ordinal pairing, yaitu 15 orang diberikan pelatihan side jump sprint, 15 orang diberikan pelatihan scissor jump, dan 15 orang kelompok kontrol. Daya ledak otot tungkai diukur dengan test vertical jump. Data yang di dapat dianalisis dengan uji F (one way anova) pada taraf signifikansi (α) = 0,05 dengan bantuan komputer program SPSS 16.0. Hasil analisis data dari kelompok perlakuan side jump sprint terjadi peningkatan daya leda kotot tungkai sebesar 0,097, pada kelompok perlakuaan scissor jump sebesar 0,136 dan pada kelompok control sebesar 0,116. Hasil one awy anova variable daya ledak otot tungkai antara kelompok perlakuan dan kelompok kontrol didapat Fhitung 67,628 dan signifikansi 0,000 yang berarti terdapat perbedaan pengaruh pelatihan side jump sprint dan scissor jump terhadap daya ledak otot tungkai. . Berdasarkan hasil uji least significant difference (LSD), kelompok pelatihan side jump sprint lebih baik pengaruhnya sebesar 44,733 dibandingkan pelatihan sciisor jump terhadap peningkatan daya ledak otot tungkai. Disimpulkan bahwa; (1) pelatihan side jump sprint dan scissor jump berpengaruh pada peningkatan daya ledak otot tungkai. (2) terdapat perbedaan pengaruh pelatihan side jump sprint dan scissor jump terhadap peningkatan daya ledak otot tungkai. (3) pelatihan side jump sprint lebih baik dari pada pelatihan scissor jump terhadap peningkatan daya ledakotot tungkai Kata Kunci : side jump sprint , scissor jump, daya ledak otot tungkai. This research aims to know the influence of side jump sprint and scissor jump towards the improvement the explosive power of limp muscle. This research is a quasi experiment with the non-randomized control group pretest post test design. The subjects were students of extracurricular participants of volley ball at SMP Negeri 3 Abiansemal academic year 2016/2017 amounted to 45 people, then divided into 3 groups using ordinal pairing technique, 15 people were given side jump sprint training, 15 people were given scissor jump training, and 15 People control group. Limb muscle explosive power is measured by a vertical jump test. The data can be analyzed by F (one way anova) test at significance level (α) = 0,05 with computer program of SPSS 16.0. The result of data analysis from side sprint treatment group happened increased limb muscle explosive power of 0.097, in scissor jump treatment group 0.136 and control group 0.116. The result of one way anova variable of explosive muscle limb power between treatment group and control group was obtained Fcount 67,628 and significance 0,000 which mean there is difference of influence of training of side jump sprint and scissor jump on explosive power of limb muscle. . Based on the least significant difference (LSD) test results, the side jump sprint training group had a better effect of 44.733 than the scissor jump training on increasing the muscle limb power. It was concluded that; (1) the training of side jump sprints and scissor jumps has an effect on increasing the muscle tone of the leg muscles. (2) there is a difference in the effect of training of side jump sprint and scissor jump on the increase of explosive power of limb muscle. (3) side jump sprint training is better than scissor jump training on increasing the limbs . keyword : side jump sprint, scissor jump, explosive muscle limb power.

scholarly journals APPLYING GRAPHIC ORGANIZERS STRATEGYON GRADE TEN STUDENTS’ VOCABULARY ACQUISITION

Lingua ◽  
2018 ◽  
Vol 1 (02) ◽  
pp. 33-44
Author(s):  
Abdillah NA ◽  
Robiatul Adawiyah
Keyword(s):  
Vocabulary Acquisition ◽  
Graphic Organizers ◽  
Control Group ◽  
Control Groups ◽  
Control Group Design ◽  
Experiment Method ◽  
Post Test ◽  
And Control ◽  
Experimental Group ◽  
One Way Anova

The purpose of this research was investigating of applying graphic organizers strategy on grade ten students’ vocabulary acquisition at SMA Martia Bhakti Bekasi, particularly to find out whether or not graphic organizers has significance effect on students’ vocabulary acquisition. The population of this research was the students on grade ten at SMA Martia Bhakti Bekasi, with the total number 225 students. Samples were gotten by adoptingcluster random sampling with the total number 60 students who is sitting on 2 classes. One of classes was assigned to an experimental group, whose students were taught using the GO strategy with five specific features of vocabulary items, they are definition, synonym, antonym, example, and using it in an example sentences. While the other class was assigned to be control group, whose students were taught with the same vocabulary items using conventional strategy. The research methodology used quasi experiment method and the research design adopted post test only control group design. After gave the treatment to the experiment and control groups, the post testwas conductedformeasuringeffectivenessof the treatment, numbering 30 items. At the end of the session, the data was analyzed using SPSS v. 21. The researcher used One-Way ANOVA for analysis. The result showed that the F observed is higher than F table (4.00, come from significant level 0.05 and df= 1/58). It means that graphic organizers strategy has significant effect on students’ vocabulary acquisition.

scholarly journals Advertising Personality Impact to Education Institution Brand Image Base on Three Hits Theory of Exposure

2015 ◽  
Vol 21 (4) ◽  
pp. 777-780
Author(s):  
Rudy Aryanto ◽  
Sisca Apriliyanti ◽  
Bachtiar H. Simamora ◽  
Idris Gautama So
Keyword(s):  
Brand Image ◽  
Block Design ◽  
Education Institution ◽  
Control Group ◽  
Randomized Block Design ◽  
Print Advertisement ◽  
Information And Communication ◽  
And Control ◽  
One Way Anova

This research conducted to examine the causal effects of two dimensional design of print advertisement communication personality in tutoring course education institution base on brand personality that beneficially applied toward tutoring education institution brand image. After conducting observation of three groups of participants with different information advertisement treatment i.e., sincerity personality, excitement personality and control group, in the longitudinal three weeks experiment using One-Way ANOVA randomized block design analysis, gathered in the context of case study. It is proven that sincerity advertisement treatment is not appropriate to apply in making information and advertisement design for this case if publish more than one time broadcast. Contrarily, for the excitement personality form of advertisement treatment appear to increase influence on the brand image aggregate for three weeks, means excitement personality design of advertisement will strengthened the three hits theory. These research outcomes, when applied to education institution will providers, will help in designing appropriate personality form of information and communication of advertisement.

SERUM PEPSINOGEN I LEVELS IN GASTRIC CANCER

2013 ◽  
pp. 43-49
Author(s):  
Thi Minh Tam Phan ◽  
Thi Thu Huong Hoang ◽  
Anh Tuyen Nguyen ◽  
Thi Phuong Anh Le ◽  
Nguyen Tuong Van Ha
Keyword(s):  
Gastric Cancer ◽  
Predictive Value ◽  
Area Under The Curve ◽  
Control Group ◽  
Serum Pepsinogen ◽  
Pepsinogen I ◽  
Cancer Group ◽  
And Control ◽  
Group Serum ◽  
Gastric Cancer Group

Objective: Identification of serum Pepsinogen I levels in gastric cancer. Materials and Methods: Serum pepsinogen I levels was measured by enzym-linked immunosorbent assay (ELISA) on 32 patients in the gastric cancer group diagnosed by endoscopy and histology and control group of 30 patients with functional dyspepsia on endoscopy Using the cut-off value: PGI ≤ 70 ng/ml for gastric cancer. Results: Median Pepsinogen I levels in gastric cancer group was 41.07 ng / ml (25% quartile: 27.83 ng / ml, 75% quartile: 61.57 ng/ml) was significantly lower in control group: 102.03 ng / ml (25% quartile: 57.63 ng / ml, 75% quartile: 129.32 ng/ml) (p<0.001). The rate of Serum Pepsinogen I (≤ 70 ng/ml) was 78.1% in patients with gastric cancer, was 26.7% in the control group. Serum Pepsinogen I test in cut-off value ≤ 70 ng/ml had a sensitivity of 78.1%, specificity 73.3%, positive predictive value of 75.8% and the predictive value negative of 75.9% (p <0.001). The results of the ROC curve: area under the curve = 0.846,p <0.0001 at the cut-of of Pepsinogen I levels in our study ≤ 50.83 ng/ml with the optimal sensitivity and specificity were 65.6% and 86.7%. Key words: serum Pepsinogen I, gastric cancer

scholarly journals Pengaruh Konsentrasi Rebusan Kelopak Bunga Rossella terhadap pH Saliva Buatan

2015 ◽  
Vol 20 (2) ◽  
pp. 156
Author(s):  
Prasetyo Adi ◽  
Ambar Puspitasari ◽  
Murni Ukhuwah I
Keyword(s):  
Treatment Group ◽  
Streptococcus Mutans ◽  
Artificial Saliva ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Ph Scale ◽  
And Control ◽  
One Way Anova

Saliva adalah sekelompok cairan oral yang kompleks. Salah satu mikroorganisme yang menyebabkan pH saliva menjadi asam adalah Streptococcus mutans. Rebusan kelopak bunga Rossella (Habiscus sabdariffa L) mengandung flavonoid dan antosianin yang dapat mengganggu aktivitas sel dan pertumbuhan Streptococcus mutans. Tujuan penelitian ini mengetahui pengaruh konsentrasi rebusan kelopak bunga Rossella terhadap pH dan absorbansi saliva buatan yang diinduksi Streptococcus mutans secara in vitro. Penelitian ini menggunakan True Experimental Design yaitu Posttest Control Group Design. Konsentrasi yang digunakan dalam penelitian ini adalah 5%, 15%, 25%, dan 35%. Hasil penelitian menunjukkan ada perbedaan nilai pH dan absorbansi saliva buatan yang telah diinduksi Streptococcus mutans dan ditambahkan dengan rebusan kelopak bunga Rossella terhadap kelompok kontrol secara in vitro. Analisa data menggunakan uji Korelasi dan Regresi menunjukkan pengaruh sebesar 99,2% pada pemberian rebusan kelopak bunga Rossella terhadap nilai pH dan absorbansi saliva buatan. Uji One-Way ANOVA menunjukkan nilai signifikansi 0,000 (p< 0,05). Disimpulkan terdapat perbedaan nilai pH dan absorbansi saliva buatan yang signifikan antara kelompok kontrol dan kelompok perlakuan.The Effect of Rossella Flower Petals Stew to Artificial Saliva pH. Saliva is a complex oral fluids. One of the microorganisms that cause the saliva’s pH becomes acid is Streptococcus mutans. Water stew of Rossella flower petals (Habiscus sabdariffa L) contains flavonoids and anthocyanins that interfere metabolism of the cells and growth of Streptococcus mutans. The purpose of this experimental was to know the effect of water stew Rossella flower petals concentration in pH scale and absorbance score of artificial saliva which induced with Streptococcus mutans by in vitro. The method which applied here was True Experimental Design, post test control group design. The concentrations that used in this study were 5%, 15%, 25% and 35%. The results described the differences in pH scale and absorbance score of artificial saliva which induced with Streptococcus mutans between treatment group (added by water stew Rossella petals) and control group. Data analysis using correlation and regression test showed the effect of water stew of Rossella flower petals in pH scale of artificial saliva was 99.2%. The conclusion of this experimental explained the increase of pH scale after added with the water stew of Rossella flower petals, also showed a significant differences of pH scale of artificial saliva between treatment group and control group based on the One-Way ANOVA test, and the water stew of Rossella flower petals was effective to maintain pH scale of artificial saliva approach normal pH scale

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