scholarly journals Proteomic analysis of human cervicovaginal fluid collected before preterm premature rupture of the fetal membranes

Reproduction ◽  
2013 ◽  
Vol 145 (2) ◽  
pp. 137-147 ◽  
Author(s):  
Stella Liong ◽  
Megan K W Di Quinzio ◽  
Yujing J Heng ◽  
Gabrielle Fleming ◽  
Michael Permezel ◽  
...  
Keyword(s):  
Binding Protein ◽  
Fetal Membranes ◽  
Two Dimensional ◽  
Protease Inhibition ◽  
Premature Rupture ◽  
Fatty Acid Binding ◽  
Elastase Inhibitor ◽  
Membrane Rupture ◽  
Preterm Premature Rupture ◽  
Cervicovaginal Fluid

A significant obstetric complication facing contemporary materno–fetal medicine is preterm premature rupture of the fetal membranes (preterm PROM), which occurs in 30% of all preterm births. The objective of this study was to identify differentially expressed proteins in the cervicovaginal fluid of asymptomatic women before the clinical manifestation of preterm PROM. The preterm PROM group comprised of women with samples collected 6–23 days before PROM, who subsequently delivered preterm (n=5). Women who spontaneously delivered at term served as gestation-matched controls (n=10). Two-dimensional difference in-gel electrophoresis was used to distinguish differential expression between the pooled groups and fold changes were subsequently confirmed by two-dimensional PAGE of individual samples. Spots of interest were identified by mass spectrometry. Proteins that were significantly reduced with impending preterm PROM included the following: thioredoxin (2.7-fold), interleukin 1 receptor antagonist (1.7-fold), fatty acid-binding protein 5 (2.1-fold), cystatin A (dimer; 1.9-fold), monocyte/neutrophil elastase inhibitor (1.6-fold), squamous cell carcinoma antigen-1 (2.1-fold) and γ-glutamyl cyclotransferase (3.0-fold). By contrast, annexin A3 (3.7-fold) and vitamin D binding protein (3.9-fold) were significantly increased with impending preterm PROM. Western blot analysis was also performed on an independent cohort of preterm PROM and control samples to validate these candidate biomarkers. These proteins have known biological functions in oxidative balance, anti-inflammatory activity, metabolism or protease inhibition that may facilitate membrane rupture.

Perinatal outcomes after previable preterm premature rupture of membranes before 24 weeks of gestation

2018 ◽  
Vol 46 (5) ◽  
pp. 555-565 ◽  
Author(s):  
Verena Kiver ◽  
Vinzenz Boos ◽  
Anke Thomas ◽  
Wolfgang Henrich ◽  
Alexander Weichert
Keyword(s):  
Survival Rate ◽  
Pregnancy Termination ◽  
Treatment Options ◽  
Latency Period ◽  
Perinatal Outcomes ◽  
Premature Rupture Of Membranes ◽  
Premature Rupture ◽  
Mortality And Morbidity ◽  
Membrane Rupture ◽  
Preterm Premature Rupture

Abstract Objective: A current descriptive assessment of perinatal outcomes in pregnancies complicated by previable preterm premature rupture of membranes (pPPROM) at <24 weeks of gestation, after expectant treatment. Study design: Maternal and short-term neonatal data were collected for patients with pPPROM. Results: Seventy-three patients with 93 fetuses were hospitalized with pPPROM at 15–24 weeks’ gestation. Among these patients, 27.4% (n=20) chose pregnancy termination, 27.4% (n=20) miscarried and 45.2% (n=33) proceeded to live births. After a median latency period of 38 days, ranging from 1 to 126 days, 24 singletons and 20 multiples were live-born, of whom 79.5% (n=35) survived the perinatal period. The main neonatal sequelae were pulmonary hypoplasia (29.5%; n=13), connatal infection (56.8%; n=25), intraventricular hemorrhage (25%; n=11; resulting in five neonatal deaths) and Potter’s syndrome (15.9%; n=7). Nine newborns died, within an average of 2.8 days (range, 1–10 days). The overall neonatal survival rate was 51.5% – including miscarriages but not elective terminations. The intact survival rate was 45.5% of all live-born neonates. Conclusions: Even with limited treatment options, overall neonatal survival is increasing. However, neonatal mortality and morbidity rates remain high. The gestational age at membrane rupture does not predict neonatal outcome.

scholarly journals Fetomaternal Outcome in Preterm Premature Rupture of Membrane

2014 ◽  
Vol 5 (1) ◽  
pp. 18-21 ◽  
Author(s):  
Manju Puri ◽  
Minakeshi Rana ◽  
Sharda Patra ◽  
Shubha Sagar Trivedi
Keyword(s):  
Gestational Age ◽  
Latency Period ◽  
Intrauterine Fetal Death ◽  
Premature Rupture ◽  
Complete Evaluation ◽  
Membrane Rupture ◽  
Preterm Premature Rupture ◽  
Maternal Morbidity And Mortality ◽  
The Difference ◽  
Prophylactic Use

Abstract This prospective observational study was carried out to study the fetomaternal outcome in women with preterm premature rupture of membrane (PPROM) between 24 and 36 weeks of gestation. Materials and methods A total of 150 pregnant women between 24 and 36 weeks gestation with PROM were subjected to detailed history and examination. Each patient was followed till her delivery and fetomaternal outcome was recorded gestation-wise between 24 and 28 weeks (n = 15), 28 and 32 weeks (n = 30), 32 and 34 weeks (n = 90), and 34 and 36 weeks (n = 15). Result The mean latency period from membrane rupture to delivery decreased from 15 days at 24 to 28 weeks, 11 days at 28 to 32 weeks to 4.4 days at 32 to 34 weeks to 2.1 days at 34 to 36 weeks. Majority of women delivered vaginally. The rate of spontaneous labor increased as the gestational age at admission increased, the difference between rate of spontaneous labor of 67% at 28 to 32 weeks and 86% at 34 to 36 weeks was statistically significant (p = 0.001). The indications for induction of labor were intrauterine fetal death, gross oligohydramnios, and clinical chorioamnionitis. The most common complication was clinical chorioamnionitis (6%) and postpartum sepsis (6%). The perinatal outcome was favorable in majority of cases and improved with the increase in gestational age at PROM. The overall perinatal mortality was 9.3%. Conclusion Management of PPROM involves complete evaluation of risks and benefits of conservative management. Wherever possible, the treatment should be directed toward conserving the pregnancy with prophylactic use of antibiotics and steroids thereby reducing fetal-maternal morbidity and mortality. However, termination of pregnancy should be considered at the earliest suspicion of chorioamnionitis. How to cite this article Rana M, patra s, Puri M, Trivedi SS. Fetomaternal Outcome in Preterm Premature Rupture of Membrane. Int J Infertil Fetal Med 2014;5(1):18-21.

Maternal Serum Haptoglobin Levels as a Marker of Preterm Premature Rupture of Membranes

2020 ◽  
Author(s):  
Gul Nihal Buyuk ◽  
Z.Asli Oskovi-Kaplan ◽  
Huseyin Durukan
Keyword(s):  
Pregnant Women ◽  
Maternal Serum ◽  
Control Group ◽  
Premature Rupture ◽  
Roc Curve Analysis ◽  
Membrane Rupture ◽  
Preterm Premature Rupture ◽  
Serum Haptoglobin ◽  
Significant Difference ◽  
The Mean

Abstract Objective We aimed to investigate the prognostic value of maternal serum haptoglobin levels in patients presenting with preterm premature rupture of fetal membranes (PPROM) during the second and the third trimesters of pregnancy. Methods In this case control study, 60 patients were recruited (30 pregnant women with PPROM between 26–34 weeks of gestation and 30 healthy, gestational-age-matched pregnant women without PPROM). White blood cell count (WBC), interleukin 6 (IL-6), C-reactive protein (CRP), sedimentation rate, and haptoglobin levels were measured. Results The mean age, gestational week, gravida, and parity of the 2 groups were statistically comparable (P>0.001). There was a statistically significant difference between the 2 groups in terms of haptoglobin values (p<0.001). The mean haptoglobin level was 115.5+33.1(mg/dl) in the PPROM group and 66.5+42.6 (mg/dl) in the control group. ROC curve analysis was performed to determine whether the level of haptoglobin alone could diagnose PPROM as an independent marker. It was shown that the level of 94.5 mg/dL for haptoglobin could indicate the diagnosis of PPROM with 80% sensitivity and specificity Conclusion Maternal serum haptoglobin levels may be a diagnostic marker for suspected PPROM cases when membrane rupture diagnosis is not accurate based on physical examination and other diagnostic tests.

The role of apoptosis in preterm premature rupture of the human fetal membranes

2013 ◽  
Vol 288 (3) ◽  
pp. 501-505 ◽  
Author(s):  
Aylin Saglam ◽  
Cinar Ozgur ◽  
Iris Derwig ◽  
Bekir Serdar Unlu ◽  
Funda Gode ◽  
...  
Keyword(s):  
Fetal Membranes ◽  
Premature Rupture ◽  
Preterm Premature Rupture

Risk factors for preterm premature rupture of fetal membranes: A multicenter case-control study

1990 ◽  
Vol 163 (1) ◽  
pp. 130-137 ◽  
Author(s):  
James H. Harger ◽  
Ann W. Hsing ◽  
Ruth E. Tuomala ◽  
Ronald S. Gibbs ◽  
Philip B. Mead ◽  
...  
Keyword(s):  
Risk Factors ◽  
Case Control Study ◽  
Case Control ◽  
Fetal Membranes ◽  
Premature Rupture ◽  
Preterm Premature Rupture ◽  
Control Study

scholarly journals Ubiquitin-Proteasome-Collagen (CUP) Pathway in Preterm Premature Rupture of Fetal Membranes

2017 ◽  
Vol 8 ◽  
Author(s):  
Xinliang Zhao ◽  
Xiaoyan Dong ◽  
Xiucui Luo ◽  
Jing Pan ◽  
Weina Ju ◽  
...  
Keyword(s):  
Fetal Membranes ◽  
Premature Rupture ◽  
Preterm Premature Rupture ◽  
Ubiquitin Proteasome
Sign in / Sign up

Export Citation Format

Share Document