scholarly journals Functional characterization and expression analysis of the androgen receptor in zebrafish (Danio rerio) testis

Reproduction ◽  
2008 ◽  
Vol 136 (2) ◽  
pp. 225-234 ◽  
Author(s):  
P P de Waal ◽  
D S Wang ◽  
W A Nijenhuis ◽  
R W Schulz ◽  
J Bogerd
Keyword(s):  
Androgen Receptor ◽  
Sertoli Cells ◽  
Target Genes ◽  
Functional Characterization ◽  
Dna Recognition ◽  
Adult Zebrafish ◽  
Pharmacological Characterization ◽  
Testis Tissue

The biological activity of androgens, important for male sexual differentiation and development, is mediated by the androgen receptor (AR) that binds to specific DNA recognition sites regulating the transcription of androgen target genes. We investigated androgen production by adult zebrafish testis tissue, and identified 11β-hydroxyandrostenedione, 11-ketoandrostenedione (OA), and 11-ketotestosterone (11-KT) as main products, and hence potential ligands, for the zebrafish Ar. These androgens were then included in the pharmacological characterization of the zebrafish Ar. The zebrafish Ar responded well in terms of binding and transactivation to synthetic androgens as well as to testosterone and 11-KT, and reasonably well to OA and androstenedione. In situ hybridization analysis of zebrafish testis revealed that ar mRNA expression was detected in the subpopulation of Sertoli cells contacting early spermatogonia.

scholarly journals Evolutionary History and Functional Characterization of Androgen Receptor Genes in Jawed Vertebrates

Endocrinology ◽  
2009 ◽  
Vol 150 (12) ◽  
pp. 5415-5427 ◽  
Author(s):  
Yukiko Ogino ◽  
Hironori Katoh ◽  
Shigehiro Kuraku ◽  
Gen Yamada
Keyword(s):  
Androgen Receptor ◽  
Evolutionary History ◽  
Functional Characterization

scholarly journals Molecular and functional characterization of somatostatin-type signalling in a deuterostome invertebrate

Open Biology ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 200172
Author(s):  
Ya Zhang ◽  
Luis Alfonso Yañez Guerra ◽  
Michaela Egertová ◽  
Cleidiane G. Zampronio ◽  
Alexandra M. Jones ◽  
...  
Keyword(s):  
Functional Characterization ◽  
The Body ◽  
Pharmacological Characterization ◽  
Cardiac Stomach ◽  
Physiological Processes ◽  
Pharmacological Tests ◽  
The Central Nervous System

Somatostatin (SS) and allatostatin-C (ASTC) are structurally and evolutionarily related neuropeptides that act as inhibitory regulators of physiological processes in mammals and insects, respectively. Here, we report the first molecular and functional characterization of SS/ASTC-type signalling in a deuterostome invertebrate—the starfish Asterias rubens (phylum Echinodermata). Two SS/ASTC-type precursors were identified in A. rubens (ArSSP1 and ArSSP2) and the structures of neuropeptides derived from these proteins (ArSS1 and ArSS2) were analysed using mass spectrometry. Pharmacological characterization of three cloned A. rubens SS/ASTC-type receptors (ArSSR1–3) revealed that ArSS2, but not ArSS1, acts as a ligand for all three receptors. Analysis of ArSS2 expression in A. rubens using mRNA in situ hybridization and immunohistochemistry revealed stained cells/fibres in the central nervous system, the digestive system (e.g. cardiac stomach) and the body wall and its appendages (e.g. tube feet). Furthermore, in vitro pharmacological tests revealed that ArSS2 causes dose-dependent relaxation of tube foot and cardiac stomach preparations, while injection of ArSS2 in vivo causes partial eversion of the cardiac stomach. Our findings provide new insights into the molecular evolution of SS/ASTC-type signalling in the animal kingdom and reveal an ancient role of SS-type neuropeptides as inhibitory regulators of muscle contractility.

Pharmacological characterization of an imidazolopyrazole as novel selective androgen receptor modulator

2013 ◽  
Vol 134 ◽  
pp. 51-58 ◽  
Author(s):  
Xuqing Zhang ◽  
George F. Allan ◽  
Pamela Tannenbaum ◽  
Tifanie Sbriscia ◽  
Olivia Linton ◽  
...  
Keyword(s):  
Androgen Receptor ◽  
Selective Androgen Receptor Modulator ◽  
Pharmacological Characterization ◽  
Receptor Modulator
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