Phenotypic and Functional Characterization of Ductal Carcinoma In Situ–Associated Myoepithelial Cells

2015 ◽  
Vol 15 (5) ◽  
pp. 335-342 ◽  
Author(s):  
Manish Rohilla ◽  
Amanjit Bal ◽  
Gurpreet Singh ◽  
Kusum Joshi
Keyword(s):  
Ductal Carcinoma In Situ ◽  
Carcinoma In Situ ◽  
Ductal Carcinoma ◽  
Functional Characterization ◽  
Myoepithelial Cells

scholarly journals Characterization of ductal carcinoma in situ cell lines established from breast tumor of a Singapore Chinese patient

2014 ◽  
Vol 14 (1) ◽  
Author(s):  
Jacklyn WY Yong ◽  
Meng Ling Choong ◽  
SiFang Wang ◽  
Yu Wang ◽  
Shermaine QY Lim ◽  
...  
Keyword(s):  
Cell Lines ◽  
Ductal Carcinoma In Situ ◽  
Breast Tumor ◽  
Carcinoma In Situ ◽  
Ductal Carcinoma ◽  
Chinese Patient ◽  
Singapore Chinese

scholarly journals Perturbed myoepithelial cell differentiation in BRCA mutation carriers and in ductal carcinoma in situ

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Lina Ding ◽  
Ying Su ◽  
Anne Fassl ◽  
Kunihiko Hinohara ◽  
Xintao Qiu ◽  
...  
Keyword(s):  
Germline Mutation ◽  
Ductal Carcinoma In Situ ◽  
Carcinoma In Situ ◽  
Ductal Carcinoma ◽  
Myoepithelial Cells ◽  
Normal Breast ◽  
Normal Mammary Gland ◽  
Mutation Carriers ◽  
Breast Tissues

Abstract Myoepithelial cells play key roles in normal mammary gland development and in limiting pre-invasive to invasive breast tumor progression, yet their differentiation and perturbation in ductal carcinoma in situ (DCIS) are poorly understood. Here, we investigated myoepithelial cells in normal breast tissues of BRCA1 and BRCA2 germline mutation carriers and in non-carrier controls, and in sporadic DCIS. We found that in the normal breast of non-carriers, myoepithelial cells frequently co-express the p63 and TCF7 transcription factors and that p63 and TCF7 show overlapping chromatin peaks associated with differentiated myoepithelium-specific genes. In contrast, in normal breast tissues of BRCA1 mutation carriers the frequency of p63+TCF7+ myoepithelial cells is significantly decreased and p63 and TCF7 chromatin peaks do not overlap. These myoepithelial perturbations in normal breast tissues of BRCA1 germline mutation carriers may play a role in their higher risk of breast cancer. The fraction of p63+TCF7+ myoepithelial cells is also significantly decreased in DCIS, which may be associated with invasive progression.

Characterization of ductal carcinoma in situ on diffusion weighted breast MRI

2011 ◽  
Vol 21 (9) ◽  
pp. 2011-2019 ◽  
Author(s):  
Habib Rahbar ◽  
Savannah C. Partridge ◽  
Peter R. Eby ◽  
Wendy B. DeMartini ◽  
Robert L. Gutierrez ◽  
...  
Keyword(s):  
Ductal Carcinoma In Situ ◽  
Carcinoma In Situ ◽  
Ductal Carcinoma ◽  
Breast Mri ◽  
Diffusion Weighted

scholarly journals Molecular and cellular characterization of two patient-derived ductal carcinoma in situ (DCIS) cell lines, ETCC-006 and ETCC-010

BMC Cancer ◽  
2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Julia Samson ◽  
Magdalina Derlipanska ◽  
Oza Zaheed ◽  
Kellie Dean
Keyword(s):  
Breast Cancer ◽  
Cell Cycle ◽  
Cell Line ◽  
Cell Lines ◽  
Ductal Carcinoma In Situ ◽  
Carcinoma In Situ ◽  
Ductal Carcinoma ◽  
Anchorage Independent Growth

Abstract Background Currently it is unclear how in situ breast cancer progresses to invasive disease; therefore, a better understanding of the events that occur during the transition to invasive carcinoma is warranted. Here we have conducted a detailed molecular and cellular characterization of two, patient-derived, ductal carcinoma in situ (DCIS) cell lines, ETCC-006 and ETCC-010. Methods Human DCIS cell lines, ETCC-006 and ETCC-010, were compared against a panel of cell lines including the immortalized, breast epithelial cell line, MCF10A, breast cancer cell lines, MCF7 and MDA-MB-231, and another DCIS line, MCF10DCIS.com. Cell morphology, hormone and HER2/ERBB2 receptor status, cell proliferation, survival, migration, anchorage-independent growth, indicators of EMT, cell signalling pathways and cell cycle proteins were examined using immunostaining, immunoblots, and quantitative, reverse transcriptase PCR (qRT-PCR), along with clonogenic, wound-closure and soft agar assays. RNA sequencing (RNAseq) was used to provide a transcriptomic profile. Results ETCC-006 and ETCC-010 cells displayed notable differences to another DCIS cell line, MCF10DCIS.com, in terms of morphology, steroid-receptor/HER status and markers of EMT. The ETCC cell lines lack ER/PR and HER, form colonies in clonogenic assays, have migratory capacity and are capable of anchorage-independent growth. Despite being isogenic, less than 30% of differentially expressed transcripts overlapped between the two lines, with enrichment in pathways involving receptor tyrosine kinases and DNA replication/cell cycle programs and in gene sets responsible for extracellular matrix organisation and ion transport. Conclusions For the first time, we provide a molecular and cellular characterization of two, patient-derived DCIS cell lines, ETCC-006 and ETCC-010, facilitating future investigations into the molecular basis of DCIS to invasive ductal carcinoma transition.

Ultrasonographic detection and characterization of asymptomatic ductal carcinoma in situ with histopathologic correlation

2011 ◽  
Vol 52 (4) ◽  
pp. 364-371 ◽  
Author(s):  
Yeon Ju Gwak ◽  
Hye Jung Kim ◽  
Jin Young Kwak ◽  
Sang Kwon Lee ◽  
Kyung Min Shin ◽  
...  
Keyword(s):  
Ductal Carcinoma In Situ ◽  
Carcinoma In Situ ◽  
Ductal Carcinoma

Characterization of High-grade Ductal Carcinoma In Situ With and Without Regressive Changes

2009 ◽  
Vol 17 (6) ◽  
pp. 495-499 ◽  
Author(s):  
Mamatha Chivukula ◽  
Akosua Domfeh ◽  
Gloria Carter ◽  
George Tseng ◽  
David J. Dabbs
Keyword(s):  
Ductal Carcinoma In Situ ◽  
Carcinoma In Situ ◽  
Ductal Carcinoma ◽  
High Grade ◽  
Regressive Changes

Histopathological characterization of ductal carcinoma in situ (DCIS) of the breast according to HER2 amplification status and molecular subtype

2014 ◽  
Vol 465 (3) ◽  
pp. 275-289 ◽  
Author(s):  
Mieke Van Bockstal ◽  
Kathleen Lambein ◽  
Hannelore Denys ◽  
Geert Braems ◽  
Ann Nuyts ◽  
...  
Keyword(s):  
Ductal Carcinoma In Situ ◽  
Carcinoma In Situ ◽  
Ductal Carcinoma ◽  
Molecular Subtype ◽  
Her2 Amplification
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