scholarly journals Effect of dihydrotestosterone on the growth and function of ovarian follicles in intact immature female rats primed with PMSG

Reproduction ◽  
1990 ◽  
Vol 90 (1) ◽  
pp. 267-277 ◽  
Author(s):  
B. A. Conway ◽  
V. B. Mahesh ◽  
T. M. Mills
Keyword(s):  
Ovarian Follicles ◽  
Female Rats ◽  
Immature Female ◽  
And Function

Immunohistochemical localization of inhibin α- and βA-subunits in the ovary of immature female rats

1995 ◽  
Vol 132 (1) ◽  
pp. 97-102 ◽  
Author(s):  
Mitsuko Kobayashi ◽  
Mareo Yamoto ◽  
Sawako Minami ◽  
Miyako Imai ◽  
Ryosuke Nakano
Keyword(s):  
Granulosa Cells ◽  
Ovarian Follicles ◽  
Immunohistochemical Localization ◽  
Female Rats ◽  
Positive Staining ◽  
Immature Female ◽  
Immature Rat ◽  
Medical College ◽  
Thecal Cells ◽  
Rat Ovary

Kobayashi M, Yamoto M, Minami S, Imai M, Nakano R. Immunohistochemical localization of inhibin α- and βA-subunits in the ovary of immature female rats. Eur J Endocrinol 1995;132:97–102. ISSN 0804–4643. Immunohistochemical localization of inhibin α- and βA-subunits was examined in the ovaries of immature female rats. The granulosa cells in various sized ovarian follicles obtained from rats that were 10–24 days old exhibited positive staining for inhibin α- and βA-subunits. The relative intensities of immunostaining for α- and βA-subunits increased during follicular growth and maturation. Ova and internal thecal cells did not show any immunostaining for inhibin α- and/or βA-subunits. These results suggest that granulosa cells of immature rat ovaries may produce inhibin from the 10th day after birth, and that an increase in the number of mature ovarian follicles results in an increase in inhibin production in the immature rat ovary during prepubertal development. Mitsuko Kobayashi, Department of Obstetrics and Gynaecology, Wakayama Medical College, 1 shichibancho, Wakayama 640, Japan

Gene expression profile of rat ovarian tissue following xenotransplantation into immune-deficient mice

Reproduction ◽  
2009 ◽  
Vol 137 (6) ◽  
pp. 957-967 ◽  
Author(s):  
Cansu Agca ◽  
Mathew C Lucy ◽  
Yuksel Agca
Keyword(s):  
Gene Expression ◽  
Ovarian Tissue ◽  
Mammalian Species ◽  
Female Rats ◽  
Immature Female ◽  
Kidney Capsule ◽  
Ovarian Metabolism ◽  
And Function ◽  
Sexually Mature ◽  
Deficient Mice

Immune-compromised mice have been used as gonadal tissue recipients to develop gametes of various mammalian species. The aim of this research was to determine gene expression differences between fresh and frozen–thawed rat xenotransplanted (XT) ovaries as well the gene expression differences between XT and sexually mature rat ovaries that were non-transplanted (NT). Ovaries from sexually immature female rats were transplanted under the kidney capsule of ovariectomized athymic nude mice either fresh or after freezing. The XT ovaries were collected ∼10–12 weeks after xenografting for microarray analysis. The NT ovaries were collected from sexually mature rats. Gene expression was very similar between fresh and cryopreserved XT ovaries: 125 genes were twofold up- or downregulated, but level of regulation was not statistically significant. Overall patterns of gene expression between XT and NT ovaries were very different indicated by the absence of diagonal relationship between XT and NT ovary gene expression. More than 3000 genes were significantly (P<0.01) up- or downregulated between XT and NT ovaries. Genes involved in metabolic processes, lipid metabolism, and growth were downregulated in XT ovaries, whereas genes involved in immune and inflammatory response were upregulated in XT ovaries. The results showed that ovarian tissue xenografting significantly alters genes responsible for ovarian metabolism and function and leads to an upregulation of genes responsible for graft rejection.

The Fine Structure of Rat Granulosa Cell Cultures Correlated with Progestin Secretion

1973 ◽  
Vol 31 ◽  
pp. 552-553
Author(s):  
T. M. Crisp ◽  
F.R. Denys
Keyword(s):  
Fine Structure ◽  
Granulosa Cell ◽  
Cell Cultures ◽  
Single Injection ◽  
Surrounding Medium ◽  
Petri Dish ◽  
Female Rats ◽  
Vaginal Smear ◽  
Immature Female ◽  
Serum Gonadotropin

The purpose of this paper is to present observations on the fine structure of rat granulosa cell cultures grown in the presence of an adenohypophyseal explant and to correlate the morphology of these cells with progestin secretion. Twenty-six day old immature female rats were given a single injection of 5 IU pregnant mares serum gonadotropin (PMS) in order to obtain ovaries with large vesicular follicles. At 66 hrs. post-PMS administration (estrus indicated by vaginal smear cytology), the ovaries were removed and placed in a petri dish containing medium 199 and 100 U penicillin/streptomycin (P/S)/ml. Under a 20X magnification dissecting microscope, some 5-8 vesicular follicles/ovary were punctured and the granulosa cells were expressed into the surrounding medium. The cells were transferred to centrifuge tubes and spun down at 1000 rpm for 5 mins.

Effects of the aqueous extract of Dicliptera verticillata on fertility and different stages of gestation in female rats

Zygote ◽  
2021 ◽  
pp. 1-7
Author(s):  
Stéphanie Ouabo Meguem ◽  
Landry Lienou Lienou ◽  
Marie Stéphanie Chekem Goka ◽  
Richard Simo Tagne ◽  
Didiane Mefokou Yemele ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Aqueous Extract ◽  
Implantation Rate ◽  
The Other ◽  
Female Rats ◽  
High Dose ◽  
Immature Female ◽  
Implantation Sites ◽  
Parameters Analysis ◽  
Hepatic Proteins

Summary Dicliptera verticillata is a medicinal plant traditionally used in western Cameroon to cure female infertility. This experiment was designed to assess the effects of the aqueous extract of Dicliptera verticillata (AEDv) on fertility and gestation in female rats. Oral increasing doses of AEDv were administered to immature female rats over 20 d. After this time, some animals were mated with fertile males and some fertility parameters were assayed; the other animals were euthanized for preliminary toxicity parameters analysis. The effects of AEDv on the different stages of gestation were assayed on selected animals previously controlled for estrous cycle regularity and mated. AEDv led to an increase in serum, uterine and ovarian proteins as well as in ovarian and uterine weights (P < 0.05) in immature female rats. Hepatic proteins significantly decreased (P < 0.01) in high dose-treated animals (50 and 100 mg/kg) compared with controls. The number of implantation sites and the fertility rate were significantly lower (P < 0.05), while the antifertility activity increased significantly (P < 0.05) in treated rats compared with controls. When administered from the 1st to the 5th day of pregnancy, AEDv led to a decrease of more than 60% in the implantation rate in high dose-treated rats (50, 100, and 400 mg/kg). From the 6th to the 9th day, the implantation, gestation rates and the number of fetuses decreased significantly in all treated groups. From the 11th to the 20th day, a 50% resorption and decrease in gestation rate were reported in 50 mg/kg dose-treated animals. AEDv possesses weak contraceptive and abortifacient effects during pregnancy.

STEROL SYNTHESIS IN RATS WITH EXPERIMENTALLY-INDUCED POLYCYSTIC OVARIES

1967 ◽  
Vol 39 (1) ◽  
pp. 53-60 ◽  
Author(s):  
W. C. ADAMS ◽  
J. H. LEATHEM
Keyword(s):  
Serum Cholesterol ◽  
Total Content ◽  
Gonadal Hormones ◽  
Female Rats ◽  
Cholesterol Concentration ◽  
Sterol Synthesis ◽  
Sterol Metabolism ◽  
Polycystic Ovaries ◽  
Immature Female

SUMMARY Immature female rats were fed thiouracil for 30 days and injected with 10 i.u. human chorionic gonadotrophin (HCG) for the last 20 days. In thiouracil-fed animals, HCG produced large ovaries containing follicular cysts. These ovaries showed a subnormal concentration of cholesterol but both a normal total content and normal incorporation of [1-14C]acetate into digitonin-precipitable-sterols. Liver and serum cholesterol concentrations were reduced, but in vivo, 4 hr. incorporation of acetate into sterols was doubled suggesting either an acceleration of cholesterol turnover or delayed utilization of sterol precursors of cholesterol. HCG also reduced ovarian cholesterol concentration in euthyroid animals but total organ content and incorporation of [14C]acetate were not altered, nor were liver and serum cholesterol affected. Since the effect of induced ovarian cysts on sterol metabolism cannot be accounted for by known effects of thyroid or gonadal hormones it is suggested that influences of steroid hormones on lipid metabolism may be greatly modified in thyroid deficiency.

IN-VITRO STUDIES OF THE EFFECTS OF OESTROGEN PRETREATMENT ON THE SENSITIVITY OF THE IMMATURE FEMALE RAT PITUITARY GLAND TO STIMULATION WITH GONADOTROPHIN RELEASING HORMONE

1977 ◽  
Vol 74 (1) ◽  
pp. 11-21 ◽  
Author(s):  
M. WILKINSON ◽  
D. DE ZIEGLER ◽  
DANIELLE CASSARD ◽  
K. B. RUF
Keyword(s):  
Pituitary Gland ◽  
Brain Lesion ◽  
Culture Technique ◽  
Female Rats ◽  
Female Rat ◽  
Immature Female ◽  
Releasing Hormone ◽  
Gonadotrophin Releasing Hormone ◽  
Unilateral Brain Lesion

The effects of oestrogen priming on the sensitivity of the anterior pituitary gland to stimulation with gonadotrophin releasing hormone (GnRH) was investigated in immature female rats using a new organ culture technique. Hemipituitary glands obtained from animals primed with a single dose of oestradiol benzoate (OB; 20 μg/100 g body weight) released significantly more LH when pulsed with GnRH (4 nmol/l) than did control hemipituitary glands. This potentiating effect was detectable as early as 5 days after birth. After a second stimulation, LH secretion remained high. These results were compared with those obtained from animals treated to induce increased levels of endogenous oestrogen on day 26 of life. Thus, hemipituitary glands were obtained from animals given two injections of OB, an injection of pregnant mare serum gonadotrophin (PMSG) or a unilateral brain lesion placed in the basal hypothalamus. Pituitary tissue was stimulated as before with a pulse of GnRH. Two injections of OB enhanced the sensitivity to stimulation. Conversely, both PMSG and lesion treatment severely reduced the sensitivity to GnRH, although PMSG-treated and lesioned animals have been used as models for the study of ovulation.

Effects of genistein on gonadotropic cells in immature female rats

2012 ◽  
Vol 114 (3) ◽  
pp. 270-275 ◽  
Author(s):  
Ivana Medigović ◽  
Milica Manojlović-Stojanoski ◽  
Svetlana Trifunović ◽  
Nataša Ristić ◽  
Verica Milošević ◽  
...  
Keyword(s):  
Female Rats ◽  
Immature Female ◽  
Gonadotropic Cells

scholarly journals Effects of Ovariectomy on Skeletal Muscle Structure and Function in Young Female Rats: Protective Effects of the Flavanol (−)‐Epicatechin

2020 ◽  
Vol 34 (S1) ◽  
pp. 1-1
Author(s):  
Viridiana Navarrrete ◽  
Marcos Ayala ◽  
Antonio Rodriguez ◽  
Francisco Villarreal ◽  
Israel Ramirez-Sanchez
Keyword(s):  
Skeletal Muscle ◽  
Young Female ◽  
Structure And Function ◽  
Female Rats ◽  
Protective Effects ◽  
Muscle Structure ◽  
And Function
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