scholarly journals Resolution of the sperm motility-stimulating principle of fowl seminal plasma into Ca2+ and an unidentified low molecular weight factor

Reproduction ◽  
1987 ◽  
Vol 81 (2) ◽  
pp. 495-499 ◽  
Author(s):  
K. Ashizawa ◽  
G. J. Wishart
Keyword(s):  
Molecular Weight ◽  
Sperm Motility ◽  
Seminal Plasma ◽  
Low Molecular Weight ◽  
Weight Factor

scholarly journals Bovine Seminal Plasma Contains a Low-Molecular-Weight Factor that Inhibits RNA Synthesis

1984 ◽  
Vol 13 (2-3) ◽  
pp. 261-267 ◽  
Author(s):  
G. Lugaro ◽  
E. Manera ◽  
M. M. Casellato ◽  
G. Bernasconi ◽  
M. Sanso ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Seminal Plasma ◽  
Rna Synthesis ◽  
Low Molecular Weight ◽  
Weight Factor

Low molecular weight components in bovine semen diffusate and their effects on motility of bull sperm

1994 ◽  
Vol 6 (2) ◽  
pp. 165 ◽  
Author(s):  
N al-Somai ◽  
R Vishwanath ◽  
P Shannon ◽  
PC Molan
Keyword(s):  
Molecular Weight ◽  
Sperm Motility ◽  
Seminal Plasma ◽  
Detrimental Effect ◽  
Gel Permeation Chromatography ◽  
Low Molecular Weight ◽  
Small Molecular Weight ◽  
Bovine Semen ◽  
Molecular Weights ◽  
Sperm Survival

Dialysis of diluted semen before cryopreservation is beneficial to sperm survival. This is due to removal of low molecular weight components from seminal plasma that are damaging to sperm. The apparent molecular weights (M(r)) of these components range between 1000 and 12,000 as estimated by gel permeation chromatography and electrophoresis. The detrimental effect on sperm motility is greatest with the components of M(r) between 5000 and 12,000 and with those of M(r) < 1500. Their effect on sperm motility was dependent on concentration. The small molecular weight components were derived from the high molecular weight components of seminal plasma through disaggregation under prescribed conditions.

scholarly journals Specific Seminal Plasma Fractions Are Responsible for the Modulation of Sperm–PMN Binding in the Donkey

Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1388
Author(s):  
Jordi Miró ◽  
Jaime Catalán ◽  
Henar Marín ◽  
Iván Yánez-Ortiz ◽  
Marc Yeste
Keyword(s):  
Molecular Weight ◽  
Sperm Motility ◽  
Seminal Plasma ◽  
Potential Effect ◽  
Polymorphonuclear Neutrophils ◽  
Low Fertility ◽  
Plasma Fractions ◽  
Complex Fluid ◽  
First Time

While artificial insemination (AI) with frozen-thawed sperm results in low fertility rates in donkeys, the addition of seminal plasma, removed during cryopreservation, partially counteracts that reduction. Related to this, an apparent inflammatory reaction in jennies is induced following AI with frozen-thawed sperm, as a high amount of polymorphonuclear neutrophils (PMN) are observed within the donkey uterus six hours after AI. While PMN appear to select the sperm that ultimately reach the oviduct, two mechanisms, phagocytosis and NETosis, have been purported to be involved in that clearance. Remarkably, sperm interacts with PMN, but the presence of seminal plasma reduces that binding. As seminal plasma is a complex fluid made up of different molecules, including proteins, this study aimed to evaluate how different seminal plasma fractions, separated by molecular weight (<3, 3–10, 10–30, 30–50, 50–100, and >100 kDa), affect sperm–PMN binding. Sperm motility, viability, and sperm–PMN binding were evaluated after 0 h, 1 h, 2 h, 3 h, and 4 h of co-incubation at 38 °C. Two seminal plasma fractions, including 30–50 kDa or 50–100 kDa proteins, showed the highest sperm motility and viability. As viability of sperm not bound to PMN after 3 h of incubation was the highest in the presence of 30–50 and 50–100 kDa proteins, we suggest that both fractions are involved in the control of the jenny’s post-breeding inflammatory response. In conclusion, this study has shown for the first time that specific fractions rather than the entire seminal plasma modulate sperm–PMN binding within the donkey uterus. As several proteins suggested to be involved in the control of post-AI endometritis have a molecular weight between 30 and 100 kDa, further studies aimed at determining the identity of these molecules and evaluating their potential effect in vivo are much warranted.

scholarly journals A Low-Molecular-Weight Factor in Human Milk Whey Promotes Iron Uptake by Caco-2 Cells

2004 ◽  
Vol 134 (1) ◽  
pp. 93-98 ◽  
Author(s):  
Paz Etcheverry ◽  
Dennis D. Miller ◽  
Raymond P. Glahn
Keyword(s):  
Molecular Weight ◽  
Human Milk ◽  
Iron Uptake ◽  
Low Molecular Weight ◽  
Weight Factor ◽  
Milk Whey
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