scholarly journals THE INFLUENCE OF FEED RESTRICTION AND SUBSEQUENT RE-FEEDING ON GONADOTROPHIN SECRETION AND SERUM TESTOSTERONE LEVELS IN MALE RATS

Reproduction ◽  
1975 ◽  
Vol 44 (3) ◽  
pp. 429-436 ◽  
Author(s):  
B. E. HOWLAND
Keyword(s):  
Serum Testosterone ◽  
Male Rats ◽  
Feed Restriction ◽  
Testosterone Levels ◽  
Gonadotrophin Secretion

scholarly journals The Production of Testosterone and Gene Expression in Neonatal Testes of Rats Exposed to Diisoheptyl Phthalate During Pregnancy is Inhibited

2021 ◽  
Vol 12 ◽  
Author(s):  
Bin Ji ◽  
Zina Wen ◽  
Chaobo Ni ◽  
Qiqi Zhu ◽  
Yiyan Wang ◽  
...  
Keyword(s):  
Leydig Cell ◽  
Corn Oil ◽  
Serum Testosterone ◽  
Male Rats ◽  
Sprague Dawley ◽  
Testosterone Levels ◽  
Protein Levels ◽  
Gestational Exposure ◽  
Phthalate Plasticizer ◽  
Neonatal Testis

Background: Diisoheptyl phthalate (DIHP) is a phthalate plasticizer, which is a branched phthalate. Here, we reported the effects of gestational exposure to DIHP on testis development in male rats.Methods: Pregnant Sprague-Dawley rats were orally fed with vehicle (corn oil, control) or DIHP (10, 100, 500, and 1,000 mg/kg) from gestational day (GD) 12–21. At GD21, serum testosterone levels, the number and distribution of fetal Leydig cells, and testicular mRNA and protein levels, the incidence of multinucleated gonocytes, and focal testicular hypoplasia in the neonatal testis were measured.Results: DIHP increased the fetal Leydig cell cluster size and decreased the fetal Leydig cell size with LOAEL of 10 mg/kg. DIHP did not affect the fetal Leydig cell number. DIHP significantly lowered serum testosterone levels, down-regulated the expression of steroidogenesis-related genes (Lhcgr, Star, Cyp11a1, Hsd3b1, Cyp17a1, and Hsd17b3) and testis descent-related gene (Insl3) as well as protein levels of cholesterol side-chain cleavage enzyme (CYP11A1) and insulin-like 3 (INSL3). DIHP dose-dependently increased the percentage of multinucleated gonocytes with the low observed adverse-effect level (LOAEL) of 100 mg/kg. DIHP induced focal testicular hypoplasia.Conclusion: Gestational exposure to DIHP causes testis dysgenesis in rats.

scholarly journals Evaluation of The Effect of Pasak Bumi (Eurycoma longifolia Jack) to Increase Androgen Levels

INSIST ◽  
2016 ◽  
Vol 1 (1) ◽  
Author(s):  
Hurip Pratomo
Keyword(s):  
Serum Testosterone ◽  
Female Rats ◽  
Male Rats ◽  
Male Rat ◽  
Testosterone Levels ◽  
Eurycoma Longifolia ◽  
Testosterone Administration ◽  
Eurycoma Longifolia Jack ◽  
Boiled Water ◽  
Androgen Levels

Abstract—Background:The ability of reproductive disorders in men and experimental male rat is characterized among other things by a decrease in androgen such as testosterone levels. Materials of pasak bumi root (Eurycoma longifolia Jack) increased libido behavior of experimental male rat. Libido associated with higher levels of testosterone, it is therefore necessary to evaluate the effect of pasak bumi on androgen levels such as testosterone. Methods: Male rats at 9.00 am treated controls and pasak bumi, then at 14.00 pm tempted with estrus female rats 10 minutes. Then, both control and pasak bumi blood  samples were collected from male rat heart on day 1st  and day 3rd  at 14.15 pm. Measurement of testosterone levels were calculated using radioimmunoassay (RIA). Results: An increase in serum  testosterone levels in the blood on day-3rd  administration pasak bumi boiled water 18 mg/200 g dose  significantly different than the  control within day 1st to 3rd. The average levels of testosterone administration control (boiled water of aquades) day 1st = 0.50 ng/ml, day 3rd = 2.46 ng / ml, increased markedly on pasak bumi treatment day 1st = 4.00 ng / ml and day 3 = 9.73 ng / ml, (Duncan test, α=0.05). Conclusions: Testosterone levels can be increased markedly after the consumption of the pasak bumi boiled water for 3 days.Keywords—Pasak bumi, a dose of  18 mg/200 g, testosterone.

scholarly journals Evaluation of The Effect of Pasak Bumi (Eurycoma longifolia Jack) to Increase Androgen Levels

INSIST ◽  
2017 ◽  
Vol 1 (1) ◽  
Author(s):  
Hurip Pratomo
Keyword(s):  
Serum Testosterone ◽  
Female Rats ◽  
Male Rats ◽  
Male Rat ◽  
Testosterone Levels ◽  
Eurycoma Longifolia ◽  
Testosterone Administration ◽  
Eurycoma Longifolia Jack ◽  
Boiled Water ◽  
Androgen Levels

Abstract—Background:The ability of reproductive disorders in men and experimental male rat is characterized among other things by a decrease in androgen such as testosterone levels. Materials of pasak bumi root (Eurycoma longifolia Jack) increased libido behavior of experimental male rat. Libido associated with higher levels of testosterone, it is therefore necessary to evaluate the effect of pasak bumi on androgen levels such as testosterone. Methods: Male rats at 9.00 am treated controls and pasak bumi, then at 14.00 pm tempted with estrus female rats 10 minutes. Then, both control and pasak bumi blood  samples were collected from male rat heart on day 1st  and day 3rd  at 14.15 pm. Measurement of testosterone levels were calculated using radioimmunoassay (RIA). Results: An increase in serum  testosterone levels in the blood on day-3rd  administration pasak bumi boiled water 18 mg/200 g dose  significantly different than the  control within day 1st to 3rd. The average levels of testosterone administration control (boiled water of aquades) day 1st = 0.50 ng/ml, day 3rd = 2.46 ng / ml, increased markedly on pasak bumi treatment day 1st = 4.00 ng / ml and day 3 = 9.73 ng / ml, (Duncan test, α=0.05). Conclusions: Testosterone levels can be increased markedly after the consumption of the pasak bumi boiled water for 3 days.Keywords—Pasak bumi, a dose of  18 mg/200 g, testosterone.

scholarly journals Effect of oral injection of tramadol, sildenafil and combined of tramadol and sildenafil on serum testosterone level for Wistar breed male rats

2013 ◽  
Vol 11 (9) ◽  
pp. 2959-2966
Author(s):  
Rabie Said Farag ◽  
Mohamed Abdel El Nawawy ◽  
Wael Mohamed Fathy ◽  
Hamada Atiaa
Keyword(s):  
Erectile Dysfunction ◽  
Serum Testosterone ◽  
Water Soluble ◽  
Pharmacological Effect ◽  
Male Rats ◽  
Absolute Bioavailability ◽  
Serum Testosterone Level ◽  
Control Group ◽  
Testosterone Levels ◽  
The Mean

Introduction:Testosterone is the main sexual hormone in human males, and has a pharmacological effect on the physiology of sexual function. It is known that suppression of testosterone in eugonadal adult males leads to reduced sexual desire and activity, and may result in erectile dysfunction. Tramadol is rapidly and almost completely absorbed after oral administration but its absolute bioavailability is only 65–70% due to first-pass metabolism. Sildenafil citrate is a water soluble citrate salt that was firstly synthesized by Pfizer in United Kingdom to treat hypertension and angina pectoris. Interestingly, this drug exhibited a different pharmacological effect, a marked penile erection, and became the first-line treatment option to erectile dysfunction. Aim of the work :the main work goal of this search was to measure and compare between mean serum testosterone concentrations after oral injection of tramadol, sildenafil , and combination of tramadol and sildenafil beside healthy subjects.Investigated Parameter: quantitative analysis of Free Testosterone ELISA Kit in serum by The Calbiotech, Inc. 10461 Austin Dr, Spring Valley, CA U.S.A., depending on McCann, D., et al 1985.Results In the present study, the results of GT group revealed that administration of tramadol 50 mg /Kg/day for 10, 20 days and 30 days indicated that the measured testosterone levels were lower than the (GC) control group, and the decrease in testosterone mean concentrations in GT3, than GT2, than GT1. In our research GS group administration of sildenafil 50 mg /Kg/day for 10, 20 and 30 days groups indicated that increase testosterone levels than that in control GC group, and there was an increase in testosterone mean concentrations in GS3, than GS2, than GS1. The present study reveled in GT&S administration of tramadol 50 mg /Kg/day combined with sildenafil 50 mg /Kg/day that the mean serum testosterone concentrations in (GT&S1), (GT&S2) and (GT&S3), were decrease than in the mean serum testosterone concentrations (GC), but this decrease in the mean serum testosterone concentrations in (GT&S1), (GT&S2) and (GT&S3), were quite higher than mean serum testosterone concentrations in GT1, GT2, and GT3, also and there were a decrease in mean testosterone mean concentrations in (GT&S3) than that in (GT&S2) and (GT&S1)conclusion: tramadol administration decrease testosterone levels, while sildenafil increase testosterone levels, while the combination between tramadol & sildenafil decrease testosterone levels.

The changes of the thyroid function and serum testosterone levels after long-term L-NAME treatment in male rats

1998 ◽  
Vol 21 (4) ◽  
pp. 234-238 ◽  
Author(s):  
M. Haluzík ◽  
J. Nedvídková ◽  
V. Kopský ◽  
J. Jahodová ◽  
B. Hořejší ◽  
...  
Keyword(s):  
Thyroid Function ◽  
Serum Testosterone ◽  
Male Rats ◽  
Testosterone Levels

scholarly journals Examination of Apoptotic and Autophagic Effects of Chronic Roflumilast Use on Rat Testicular Tissue by Immunohistochemical and Immunofluorescence Methods

2021 ◽  
Author(s):  
arzu gezer ◽  
Ebru KARADAĞ SARI
Keyword(s):  
Testosterone Level ◽  
Caspase 3 ◽  
Serum Testosterone ◽  
Testicular Tissue ◽  
Male Rats ◽  
Pde4 Inhibitor ◽  
Serum Testosterone Level ◽  
Sprague Dawley ◽  
Negative Effects ◽  
Testosterone Levels

Abstract Roflumilast (ROF) (3-cyclo-propylmethoxy-4-difuorome-thoxy-N-[3,5-di-chloropyrid-4-yl] benzamide) is a second generation and forcible phosphodiesterase-4 (PDE4) inhibitor. This study aims to investigate the effects of chronic Roflumilast in different doses on testicular tissue and testosterone levels in healthy Sprague-Dawley rats. During the research, the 6 weeks old (180–200 gr), 36 male rats were divided into 4 groups. Roflumilast (ROF) was administered as 0.5 mg/kg and 1 mg/kg by oral gavage for four weeks, once each day. Hematoxylin-Eosin (H&E) staining for histopathological examinations in testicular tissue, immunohistochemical and immunofluorescence examinations for Caspase-3, Apoptosis Inducing Factor (AIF) and Light Chain 3β (LC3B) expression levels, and ELISA method used to determine serum testosterone levels. Data were analyzed using SPSS v.22 with Kruskal-Wallis, Mann Whitney-U, and Wilcoxon tests. Roflumilast group lost weight compared to the control and sham. Shedding in the seminiferous epithelium, degenerations in the interstitial area, separation between cells, desquamation, interstitial edema and degenerative changes in the testicular tissue was observed. While apoptosis and autophagy determined by Caspase-3, AIF and LC3B were close and statistically insignificant in control and sham, there was significantly increased apoptotic and autophagic changes and immunopositivity in the ROF groups. The 1 mg/kg Roflumilast group’s serum testosterone level was lower than control, sham and 0.5 mg/kg Roflumilast groups. When the research data was evaluated, it was determined that the chronic use of the active ingredient Roflumilast, which has a broad-spectrum area such as COPD, arthritis, neurodegenerative diseases, liver and dermatology, had negative effects on the testicular tissue and testosterone level of rats.

SERUM TESTOSTERONE AS AN INDEX OF GONADOTROPHIN SECRETION IN ACROMEGALY AND PITUITARY DISEASE

1970 ◽  
Vol 65 (2) ◽  
pp. 302-308 ◽  
Author(s):  
Robert L. Rosenfield ◽  
Ira D. Goldfine ◽  
A. M. Lawrence
Keyword(s):  
Interstitial Cell ◽  
Sperm Count ◽  
Serum Testosterone ◽  
Substitution Therapy ◽  
Normal Response ◽  
Testosterone Levels ◽  
Pituitary Disease ◽  
Gonadotrophin Secretion ◽  
Lh Secretion ◽  
Stimulating Hormone

ABSTRACT Laboratory evidence for hypogonadism has been sought in 33 patients with acromegaly and pituitary disease. Low serum testosterone was found in 7 of the 15 males with acromegaly and 4 of 4 with chromophobe adenomas. Clomiphene administration caused no rise in testosterone in the acromegalic tested; a normal response to HCG, however, was determined in another. Disparity between testosterone levels and sperm count in three subjects suggests that dissociation of follicle stimulating hormone (FSH) secretion from interstitial cell stimulating hormone (LH) secretion may occur in association with hypothalamic-pituitary disease. This study indicates that gonadotrophic insufficiency is a commonly associated feature of acromegaly in the male. Testosterone levels were depressed in four of the five females with hypoadrenalism and/or hypogonadism secondary to pituitary disease who were not on oestrogen substitution therapy.

Short- and long-term effects of testosterone on diaphragm in castrated and normal male rats

1997 ◽  
Vol 82 (1) ◽  
pp. 134-143 ◽  
Author(s):  
David J. Prezant ◽  
Manoj L. Karwa ◽  
Helen H. Kim ◽  
Diane Maggiore ◽  
Virginia Chung ◽  
...  
Keyword(s):  
Serum Testosterone ◽  
Male Rats ◽  
Normal Male ◽  
Type Ii ◽  
Long Term Effects ◽  
Testosterone Levels ◽  
Diaphragm Function ◽  
Short And Long Term ◽  
Normal Males

Prezant, David J., Manoj L. Karwa, Helen H. Kim, Diane Maggiore, Virginia Chung, and David E. Valentine. Short- and long-term effects of testosterone on diaphragm in castrated and normal male rats. J. Appl. Physiol. 82(1): 134–143, 1997.—The effects of short- and long-term testosterone absence or treatment on the diaphragm were studied in castrated and sexually normal male rats. Compared with control rats (untreated normal males), testosterone absence or treatment did not significantly affect costal weight. In untreated castrated males, there were significant decreases in specific forces, type II fiber cross-sectional area, and myosin heavy chain (MHC) isoform 2B after 2.5 wk. In castrated males that received testosterone, there were significant increases in specific forces, type II total fiber proportional area, and relative expression of all adult diaphragm fast MHC isoforms (MHC-2all) after 2.5 wk. In normal males that received testosterone, the only significant finding was an increase in MHC-2B after 2.5 wk. Across all groups, there was close correlation between increases in maximum tetanic forces and MHC-2all. Changes in diaphragm function and composition were closely related to changes in serum testosterone levels at 2.5 wk. The lack of significant change in diaphragm function at 10 wk occurred despite changes in serum testosterone levels and diaphragm composition similar to those at 2.5 wk. These findings support our hypothesis that the effects of testosterone are dependent on basal circulating androgen levels and study duration.

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