scholarly journals THE EFFECT OF GONADOTROPHIN ON FOLLICLE GROWTH INITIATION IN THE NEONATAL MOUSE OVARY

Reproduction ◽  
1973 ◽  
Vol 35 (1) ◽  
pp. 139-141 ◽  
Author(s):  
H. PETERS ◽  
A. G. BYSKOV ◽  
S. LINTERN-MOORE ◽  
M. FABER ◽  
M. ANDERSEN
Keyword(s):  
Neonatal Mouse ◽  
Follicle Growth ◽  
Mouse Ovary ◽  
Growth Initiation

Proceedings: Follicle growth initiation in the immature mouse ovary: extraovarian or intraovarian control?

Reproduction ◽  
1973 ◽  
Vol 35 (3) ◽  
pp. 619-620 ◽  
Author(s):  
H Peters ◽  
A. Byskov ◽  
S Lintern-Moore ◽  
M Faber
Keyword(s):  
Follicle Growth ◽  
Mouse Ovary ◽  
Growth Initiation ◽  
Immature Mouse

scholarly journals Kit Ligand and the Somatostatin Receptor Antagonist, BIM-23627, Stimulate in Vitro Resting Follicle Growth in the Neonatal Mouse Ovary

Endocrinology ◽  
2010 ◽  
Vol 151 (3) ◽  
pp. 1299-1309 ◽  
Author(s):  
Alain Gougeon ◽  
Aurélien Delangle ◽  
Nassim Arouche ◽  
Mats Stridsberg ◽  
Jean Pierre Gotteland ◽  
...  
Keyword(s):  
Receptor Antagonist ◽  
Somatostatin Receptor ◽  
Neonatal Mouse ◽  
Follicle Growth ◽  
Mouse Ovary ◽  
Kit Ligand ◽  
Somatostatin Receptor Antagonist

scholarly journals Peroxisome Proliferator-Activated Receptor Gamma Modulator Promotes Neonatal Mouse Primordial Follicle Activation In Vitro

2020 ◽  
Vol 21 (9) ◽  
pp. 3120
Author(s):  
Sook Young Yoon ◽  
Ran Kim ◽  
Hyunmee Jang ◽  
Dong Hyuk Shin ◽  
Jin Il Lee ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Primordial Follicle ◽  
Neonatal Mouse ◽  
Peroxisome Proliferator ◽  
Follicle Growth ◽  
Peroxisome Proliferator Activated Receptor ◽  
Primordial Follicles ◽  
Primordial Follicle Activation ◽  
Follicle Activation

Peroxisome proliferator-activated receptor gamma (PPARγ) is known as a regulator of cellular functions, including adipogenesis and immune cell activation. The objectives of this study were to investigate the expression of PPARγ and identify the mechanism of primordial follicle activation via PPARγ modulators in mouse ovaries. We first measured the gene expression of PPARγ and determined its relationship with phosphatase and tensin homolog (PTEN), protein kinase B (AKT1), and forkhead box O3a (FOXO3a) expression in neonatal mouse ovaries. We then incubated neonatal mouse ovaries with PPARγ modulators, including rosiglitazone (a synthetic agonist of PPARγ), GW9662 (a synthetic antagonist of PPARγ), and cyclic phosphatidic acid (cPA, a physiological inhibitor of PPARγ), followed by transplantation into adult ovariectomized mice. After the maturation of the transplanted ovaries, primordial follicle growth activation, follicle growth, and embryonic development were evaluated. Finally, the delivery of live pups after embryo transfer into recipient mice was assessed. While PPARγ was expressed in ovaries from mice of all ages, its levels were significantly increased in ovaries from 20-day-old mice. In GW9662-treated ovaries in vitro, PTEN levels were decreased, AKT was activated, and FOXO3a was excluded from the nuclei of primordial follicles. After 1 month, cPA-pretreated, transplanted ovaries produced the highest numbers of oocytes and polar bodies, exhibited the most advanced embryonic development, and had the greatest blastocyst formation rate compared to the rosiglitazone- and GW9662-pretreated groups. Additionally, the successful delivery of live pups after embryo transfer into the recipient mice transplanted with cPA-pretreated ovaries was confirmed. Our study demonstrates that PPARγ participates in primordial follicle activation and development, possibly mediated in part by the PI3K/AKT signaling pathway. Although more studies are required, adapting these findings for the activation of human primordial follicles may lead to treatments for infertility that originates from poor ovarian reserves.

268. Suppressors of cytokine signalling (SOCS): roles in ovarian follicle activation

2008 ◽  
Vol 20 (9) ◽  
pp. 68
Author(s):  
R. Keightley ◽  
E. McLaughlin ◽  
S. D. Roman ◽  
R. L. Robker ◽  
D. L. Russell
Keyword(s):  
Granulosa Cells ◽  
Ovarian Follicle ◽  
Expression Patterns ◽  
Neonatal Mouse ◽  
Mrna Levels ◽  
Follicle Growth ◽  
Oocyte Growth ◽  
Primordial Follicles ◽  
Gene And Protein Expression ◽  
Follicle Activation

Oocytes are sequestered in primordial follicles before birth and remain quiescent in the ovary for decades, until recruited into the growing pool throughout the reproductive years. Therefore activation of follicle growth is a major biological checkpoint that controls female reproductive potential. However we are only just beginning to elucidate the cellular mechanisms required, for either maintenance of the quiescent primordial pool, or initiation of follicle growth. Analysis of microarray data derived from neonatal mouse ovaries indicated that members of the Suppressors of Cytokine Signalling SOCS family of proteins may play pivotal roles in folliculogenesis. We undertook a detailed analysis of gene and protein expression patterns of the eight members of the SOCS family, namely CIS and SOCS1–7, within adult and neonatal mouse ovaries. Quantitative real time PCR and immunohistochemistry was performed to determine mRNA levels and cellular localisation in the ovaries of cycling and new born animals. SOCS proteins were expressed largely within the oocytes of developing follicles and in the granulosa cells of the larger preovulatory follicles. Expression of SOCS4 in the granulosa cells and SOCS5 within the oocyte was coincident with the activation of oocyte growth and the differentiation of squamous pregranulosa to cuboidal granulosa cells. Our investigation has identified a role for the SOCS family proteins within the ovary and SOCS4 and SOCS5 as major regulators of cytokine signalling pathways in follicle activation and development.

Regulation of foxo3 subcellular localization by kit ligand in the neonatal mouse ovary

2014 ◽  
Vol 102 (3) ◽  
pp. e117-e118
Author(s):  
M. Ezzati ◽  
M. Baker ◽  
G. Aloisio ◽  
C. Pena ◽  
Y. Nakada ◽  
...  
Keyword(s):  
Subcellular Localization ◽  
Neonatal Mouse ◽  
Mouse Ovary ◽  
Kit Ligand

FOLLICLE GROWTH IN THE IMMATURE MOUSE OVARY

1969 ◽  
Vol 62 (1) ◽  
pp. 117-132 ◽  
Author(s):  
Torben Pedersen
Keyword(s):  
Granulosa Cells ◽  
Tritiated Thymidine ◽  
List Type ◽  
Pulse Labelling ◽  
Follicle Growth ◽  
Mouse Ovary ◽  
Stage Of Development ◽  
Different Ages ◽  
Immature Mouse ◽  
Doubling Times

ABSTRACT The growth of follicles in the immature mouse ovary was investigated in autoradiographs prepared after pulse-labelling with tritiated thymidine. Three parameters, which determine follicle growth were estimated: The number of follicles present in the ovary at different ages. The time it takes follicles to grow from one stage of development to another. This was calculated from the total number of granulosa cells in these stages and from their doubling times. The doubling time of granulosa cells was determined from their labelling index and the duration of their DNA-synthesis phase. The number of follicles, which start on their development at different ages. It was found, that the follicle development is not constant in the period from birth to maturity, but varies considerably. More follicles start to grow in the young than in the older immature mouse. Moreover the follicles grow faster early in life than later. The development from a follicle with one layer of granulosa cells to one with several layers and antrum formation takes about 10 days in the first half of the immature period, while it takes about 16 days as the animal approaches maturity. It was furthermore shown, that about 850 follicles start to grow in the immature period.

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