Hypothyroidism induces oxidative stress and DNA damage in breast

2021 ◽  
Author(s):  
Milena Simões Peixoto ◽  
Andressa de Vasconcelos e Souza ◽  
Iris Soares Andrade ◽  
Carolina de Carvalho el Giusbi ◽  
Caroline Coelho Faria ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Oxidative Stress ◽  
Dna Damage ◽  
Genomic Instability ◽  
Redox Balance ◽  
Ros Generation ◽  
Antioxidant Enzyme Activities ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers ◽  
Mcf10a Cells

Breast cancer and thyroid dysfunctions have been associated for decades. Although many studies suggest a biological correlation, the mechanisms linking these two pathologies have not been elucidated. Reactive oxygen species (ROS) can oxidize lipids, proteins, and DNA molecules and may promote tumor initiation. Hence, we aimed at evaluating the mammary redox balance and genomic instability in a model of experimental hypothyroidism. Female Wistar rats were treated with 0.03% methimazole for 7 or 21 days to evaluate ROS generation, antioxidant enzyme activities, and oxidative stress biomarkers, as well as genomic instability. After 7 days, lower catalase, GPx, and DUOX activities were detected in the breast of hypothyroid group compared to the control while the levels of 4-hydroxynonenal (HNE) were higher. In addition, hypothyroid group showed an increase in γH2Ax/H2Ax ratio. 21-days hypothyroid group had increased catalase and SOD activities, without significant differences between groups in the levels of oxidative stress biomarkers and DNA damage. TSH-treated MCF10A cells showed a higher extracellular, intracellular, and mitochondrial ROS production. Additionally, greater DNA damage was observed in these cells, demonstrated by a higher comet tail DNA percentage and increased 53BP1 foci. Finally, we found that TSH treatment was not able to alter cell viability. The Genome Cancer Atlas (TGCA) data showed that high TSHR expression is associated with more invasive breast cancer types. In conclusion, we demonstrate that oxidative stress and DNA damage in breast are early events of experimental hypothyroidism. Moreover, high TSH levels induce oxidative stress and genomic instability in mammary cells.

scholarly journals Lack of Association between Nuclear Factor Erythroid-Derived 2-Like 2 Promoter Gene Polymorphisms and Oxidative Stress Biomarkers in Amyotrophic Lateral Sclerosis Patients

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Annalisa LoGerfo ◽  
Lucia Chico ◽  
Loredana Borgia ◽  
Lucia Petrozzi ◽  
Anna Rocchi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Amyotrophic Lateral Sclerosis ◽  
Gene Promoter ◽  
Redox Balance ◽  
Nuclear Factor ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers ◽  
Reducing Ability ◽  
Als Patients ◽  
Lateral Sclerosis

Oxidative stress involvement has been strongly hypothesized among the possible pathogenic mechanisms of motor neuron degeneration in amyotrophic lateral sclerosis (ALS). The intracellular redox balance is finely modulated by numerous complex mechanisms critical for cellular functions, among which the nuclear factor erythroid-derived 2-like 2 (NFE2L2/Nrf2) pathways. We genotyped, in a cohort of ALS patients(n=145)and healthy controls(n=168), three SNPs inNrf2gene promoter: −653 A/G, −651 G/A, and −617 C/A and evaluated, in a subset(n=73)of patients, advanced oxidation protein products (AOPP), iron-reducing ability of plasma (FRAP), and plasma thiols (-SH) as oxidative damage peripheral biomarkers.Nrf2polymorphisms were not different among patients and controls. Increased levels of AOPP(P<0.05)and decreased levels of FRAP(P<0.001)have been observed in ALS patients compared with controls, but no difference in -SH values was found. Furthermore, no association was found between biochemical markers of redox balance andNrf2polymorphisms. These data confirm an altered redox balance in ALS and indicate that, while being abnormally modified compared to controls, the oxidative stress biomarkers assessed in this study are independent from the −653 A/G, −651 G/A, and −617 C/ANrf2SNPs in ALS patients.

Early ROS-mediated DNA damage and oxidative stress biomarkers in Monoclonal B Lymphocytosis

2012 ◽  
Vol 317 (2) ◽  
pp. 144-149 ◽  
Author(s):  
Rosa Collado ◽  
Isabel Oliver ◽  
Carmen Tormos ◽  
Mercedes Egea ◽  
Amparo Miguel ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers ◽  
And Oxidative Stress

scholarly journals Association of oxidative stress biomarkers with adiposity and clinical staging in women with breast cancer

2015 ◽  
Vol 69 (11) ◽  
pp. 1256-1261 ◽  
Author(s):  
A A F Carioca ◽  
S M M L Verde ◽  
L A Luzia ◽  
P H C Rondó ◽  
M R D O Latorre ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Oxidative Stress ◽  
Clinical Staging ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers

scholarly journals A Novel Systematic Oxidative Stress Score Predicts the Prognosis of Patients with Operable Breast Cancer

2021 ◽  
Vol 2021 ◽  
pp. 1-14
Author(s):  
Kaiming Zhang ◽  
Liqin Ping ◽  
Tian Du ◽  
Yan Wang ◽  
Ya Sun ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Oxidative Stress ◽  
Cancer Patients ◽  
Operable Breast Cancer ◽  
Breast Cancer Patients ◽  
Training Set ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers ◽  
Validation Set ◽  
The Relationship

Background. Breast cancer was associated with imbalance between oxidation and antioxidation. Local oxidative stress in tumors is closely related to the occurrence and development of breast cancer. However, the relationship between systematic oxidative stress and breast cancer remains unclear. This study is aimed at exploring the prognostic value of systematic oxidative stress in patients with operable breast cancer. Methods. A total of 1583 operable female breast cancer patients were randomly assigned into the training set and validation set. The relationship between systematic oxidative stress biomarkers and prognosis were analyzed in the training and validation sets. Results. The systematic oxidative stress score (SOS) was established based on five systematic oxidative stress biomarkers including serum creatinine (CRE), serum albumin (ALB), total bilirubin (TBIL), lactate dehydrogenase (LDH), and blood urea nitrogen (BUN). SOS was an independent prognostic factor for operable breast cancer patients. A nomogram based on SOS and clinical characteristics could accurately predict the prognosis of operable breast cancer patients, and the area under the curve (AUC) of the nomogram was 0.823 in the training set and 0.872 in the validation set, which was much higher than the traditional prognostic indicators. Conclusions. SOS is an independent prognostic indicator for operable breast cancer patients. A prediction model based on SOS could accurately predict the outcome of operable breast cancer patients.

Plasma and erythrocyte ω-3 and ω-6 fatty acids are associated with multiple inflammatory and oxidative stress biomarkers in breast cancer

Nutrition ◽  
2019 ◽  
Vol 58 ◽  
pp. 194-200 ◽  
Author(s):  
Leticia Gomes Lira ◽  
Rute Mattos Dourado Esteves Justa ◽  
Antonio Augusto Ferreira Carioca ◽  
Sara Maria Moreira Lima Verde ◽  
Geni Rodrigues Sampaio ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Oxidative Stress ◽  
Fatty Acids ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers ◽  
And Oxidative Stress

scholarly journals Echinacoside Alleviates UVB Irradiation-Mediated Skin Damage via Inhibition of Oxidative Stress, DNA Damage, and Apoptosis

2017 ◽  
Vol 2017 ◽  
pp. 1-15 ◽  
Author(s):  
Di Zhang ◽  
Chengtao Lu ◽  
Zhe Yu ◽  
Xiayin Wang ◽  
Li Yan ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Histopathological Examination ◽  
Ultraviolet B ◽  
Ros Generation ◽  
Antioxidant Enzyme Activities ◽  
Skin Damage ◽  
Uvb Irradiation ◽  
Uvb Exposure

Ultraviolet B (UVB) irradiation has been known to cause skin damage, which is associated with oxidative stress, DNA damage, and apoptosis. Echinacoside is a phenylethanoid glycoside isolated from Herba Cistanches, which exhibits strong antioxidant activity. In this study, we evaluate the photoprotective effect of echinacoside on UVB-induced skin damage and explore the potential molecular mechanism. BALB/c mice and HaCaT cells were treated with echinacoside before UVB exposure. Histopathological examination was used to evaluate the skin damage. Cell viability, lactate dehydrogenase (LDH) levels, antioxidant enzyme activities, reactive oxygen species (ROS) generation, DNA damage, and apoptosis were measured as well. Western blot was used to measure the expression of related proteins. The results revealed that pretreatment of echinacoside ameliorated the skin injury; attenuated oxidative stress, DNA damage, and apoptosis caused by UVB exposure; and normalized the protein levels of ATR, p53, PIAS3, hnRNP K, PARP, and XPA. To summarize, echinacoside is beneficial in the prevention of UVB-induced DNA damage and apoptosis of the skin in vivo and in vitro.

scholarly journals Effect of Cruciferous Vegetable Intake on Oxidative Stress Biomarkers: Differences by Breast Cancer Status

2017 ◽  
Vol 35 (4) ◽  
pp. 277-287 ◽  
Author(s):  
Michael D. Wirth ◽  
E. Angela Murphy ◽  
Thomas G. Hurley ◽  
James R. Hébert
Keyword(s):  
Breast Cancer ◽  
Oxidative Stress ◽  
Vegetable Intake ◽  
Oxidative Stress Biomarkers ◽  
Cruciferous Vegetable ◽  
Stress Biomarkers

scholarly journals Elucidation of the liver proteome in response to an antioxidant intake in rabbits

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Akeem Babatunde Sikiru ◽  
Arunachalam Arangasamy ◽  
Stephen Sunday Acheneje Egena ◽  
Sejian Veerasamy ◽  
Ippala Janardhan Reddy ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Treatment Group ◽  
Chlorella Vulgaris ◽  
Protein Extraction ◽  
Drug Candidate ◽  
Antioxidant Enzyme Activities ◽  
Proteomic Profiling ◽  
Oxidative Stress Biomarkers ◽  
Antioxidant Genes ◽  
Stress Biomarkers

Abstract Background Antioxidant intakes are one of the most cherished dietary approaches for the management of oxidative stress-induced liver damages. These antioxidants exist as the bioactive compounds present in plants and other natural sources functioning in varieties of ways from acting as direct scavengers of the free radicals to acting as the modifiers of genes and proteins expressions. Chlorella vulgaris is one of such antioxidants; it is a unicellular microalga and a rich source of polyphenols which has been reported for its capacity of reducing oxidative stress by upregulation of antioxidant genes. However, there are scarce reports on its effect on antioxidant protein expressions and functions in the liver. This situation necessitates untargeted proteomic profiling of the liver due to the antioxidant intakes as carried out in this present study. Sixteen laboratory weaner rabbits of 8 weeks old with initial average bodyweight of 1060 ± 29.42 g were randomly divided into two groups (n = 8 per group); the first group served as control while the second served as the treatment group were used for this study. Results After a period of 120 days daily consumption of 500 mg of Chlorella vulgaris biomass per kg bodyweight of the rabbit models, the animals were sacrificed and their livers were harvested followed by protein extraction for the untargeted proteomic profiling using LC-MS/Orbitrap Fusion Tribrid™ peptides quantifier and sequencer. Also, there was an assessment of the oxidative stress biomarkers in the liver and serum of the rabbits. Five-hundred and forty-four (544) proteins were identified out of which 204 were unique to the control, 198 were unique to the treatment group, while 142 were common to both groups of the rabbits. Antioxidant proteins commonly found in both groups were upregulated in the treatment group and were significantly associated with oxidative stress-protective activities. There was a reduction in oxidative stress biomarkers of the supplemented group as indicated by the assessment of the liver malondialdehyde concentrations (p < 0.05), total antioxidant capacities (p < 0.05), and antioxidant enzyme activities (p < 0.05). Similarly, these biomarkers were significantly reduced in the serum of the supplemented rabbits (p < 0.05). Conclusion The study concluded that Chlorella vulgaris is an antioxidant agent that could be suitable for reducing liver oxidative stress damage and it is a potential drug candidate for protecting the liver against oxidative stress damages as revealed in the rabbit models.

scholarly journals Association of Anthropometric Measurements With Oxidant-Antioxidant Status Among Young Saudi Females

2018 ◽  
pp. 787-793
Author(s):  
R. LATIF ◽  
N. RAFIQUE
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Body Fat ◽  
Positive Association ◽  
Normal Weight ◽  
The Body ◽  
Anthropometric Measurements ◽  
Oxidative Stress Biomarkers ◽  
Obese Adolescent ◽  
Stress Biomarkers

Present study aimed to explore the levels and correlation of oxidative stress biomarkers with anthropometry in a population of young Saudi females. One hundred six normotensives, non-diabetic Saudi females, with minimally active lifestyle, based on their body mass index (BMI) were divided as; normal-weight (NW; n=52), overweight (OW; n=24) and obese (OB; n=30). Anthropometric measurements [BMI, Waist Circumference (WC), Waist-Hip Ratio (WHR), Body Density (BD), Body Adiposity Index (BAI), % Body fat] and oxidative stress biomarkers; Thiobarbituric acid reactive substances (TBARS), 8-hydroxy-2-deoxyguanosine (8-OH-2dG: indicative of DNA/RNA damage), Superoxide dismutase, Serum total antioxidant capacity) were recorded. There was statistically significant higher 8-OH-2dG (pg/ml) in OB compared to NW (800.63±6.19 vs. 780.22±3.34; p=0.007), as determined by one-way ANOVA and Tukey post hoc test. 8-OH-2dG was significantly and positively associated with BMI (r=0.286, p=0.004), WC (r=0.280, p=0.005), BAI (r=0.26, p=0.008), and % body fat (r=0.27, p=0.006). There may be significantly increased DNA damage in normoglycemic, normotensive obese adolescent females. This can be linked to the amount of adipose tissue in the body as depicted by strong positive association between DNA damage and BMI, WC, BAI, and % body fat.

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