Cortical bone matrix mineralisation is decreased in TRPV4 deficient male, but not in female mice

2016 ◽  
Author(s):  
Paul Roschger ◽  
Barbara Misof ◽  
Nadja Fratzl-Zelman ◽  
Joost Hoenderop ◽  
Rene Bindels ◽  
...  
Keyword(s):  
Cortical Bone ◽  
Bone Matrix ◽  
Female Mice ◽  
Matrix Mineralisation

scholarly journals Thrombospondins 1 and 2 affect lysyl oxidase protein and collagen matrix maturation in cortical bone of growing male and female mice via non-redundant pathways

2018 ◽  
Author(s):  
Dylan Shearer ◽  
Madison O Mervis ◽  
Eugene Manley ◽  
Anita B Reddy ◽  
Andrea I Alford
Keyword(s):  
Cortical Bone ◽  
Lysyl Oxidase ◽  
Bone Matrix ◽  
Collagen Matrix ◽  
Serum Starvation ◽  
Hydroxyproline Content ◽  
Male And Female ◽  
Total Signal ◽  
Female Mice ◽  
Matrix Maturation

AbstractThrombospondin-2-deficiency is associated with impaired matrix maturation in osteoblasts and cortical bone of growing mice. Here we addressed the possibility that lysyl oxidase (LOX) contributes to this phenotype. After overnight serum starvation, pro-LOX levels were elevated compared to wild-type in marrow-derived osteoblasts from male and female TSP2−/− mice. The liberated LOX pro-peptide (LOPP) was faintly visible in serum-starved cultures. When serum was maintained, pro-LOX content was not affected by TSP2 status, but relative LOPP levels were elevated in cultures from female TSP2−/− mice. Two isoforms of pro-LOX at 75 kDa and 50 kDa were detected in detergent soluble protein extracts of diaphyseal tissue from growing mice. In female mice, TSP2 status did not affect detergent soluble pro-LOX content or the relative contribution of each band to the total signal. Instead, levels of the 50 kDa band were reduced in female TSP1−/− samples. In male diaphyseal tissue, total pro-LOX content and the contribution each isoform made to the total signal was not affected by TSP1 or TSP2 status. We did not detect 32 kDa mature LOX in detergent soluble preparations of cells or whole bone tissue. Detergent insoluble hydroxyproline content was reduced in diaphyseal tissue obtained from female TSP1−/− and TSP2−/− mice. In male diaphyseal cortical samples, TSP2 but not TSP1 deficiency was associated with reduced insoluble hydroxyproline content. Our data suggest that the trimeric thrombospondins contribute to bone matrix quality via non-redundant mechanisms that are dependent on the unique tissue milieu of the male and female skeleton.

Accelerated repair of cortical bone defects using a synthetic extracellular matrix to deliver human demineralized bone matrix

2006 ◽  
Vol 24 (7) ◽  
pp. 1454-1462 ◽  
Author(s):  
Yanchun Liu ◽  
Shama Ahmad ◽  
Xiao Zheng Shu ◽  
R. Kent Sanders ◽  
Sally Anne Kopesec ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Cortical Bone ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Bone Defects ◽  
Demineralized Bone

scholarly journals Gender differences in the response of CD-1 mouse bone to parathyroid hormone: potential role of IGF-I

2006 ◽  
Vol 189 (2) ◽  
pp. 279-287 ◽  
Author(s):  
Yongmei Wang ◽  
Takeshi Sakata ◽  
Hashem Z Elalieh ◽  
Scott J Munson ◽  
Andrew Burghardt ◽  
...  
Keyword(s):  
Gender Differences ◽  
Parathyroid Hormone ◽  
Cortical Bone ◽  
Mineral Apposition Rate ◽  
Mrna Levels ◽  
Male Mice ◽  
Bone Formation Rate ◽  
Male And Female ◽  
Female Mice ◽  
Igf I

Parathyroid hormone (PTH) exerts both catabolic and anabolic actions on bone. Studies on the skeletal effects of PTH have seldom considered the effects of gender. Our study was designed to determine whether the response of mouse bone to PTH differed according to sex. As a first step, we analyzed gender differences with respect to bone mass and structural properties of 4 month old PTH treated (80 μg/kg per day for 2 weeks) male and female CD-1 mice. PTH significantly increased fat free weight/body weight, periosteal bone formation rate, mineral apposition rate, and endosteal single labeling surface, while significantly decreasing medullary area in male mice compared with vehicle treated controls, but induced no significant changes in female mice. We then analyzed the gender differences in bone marrow stromal cells (BMSC) isolated from 4 month old male and female CD-1 mice following treatment with PTH (80 μg/kg per day for 2 weeks). PTH significantly increased the osteogenic colony number and the alkaline phosphatase (ALP) activity (ALP/cell) by day 14 in cultures of BMSCs from male and female mice. PTH also increased the mRNA level of receptor activator of nuclear factor κB ligand in the bone tissue (marrow removed) of both females and males. However, PTH increased the mRNA levels of IGF-I and IGF-IR only in the bones of male mice. Our results indicate that on balance a 2-weeks course of PTH is anabolic on cortical bone in this mouse strain. These effects are more evident in the male mouse. These differences between male and female mice may reflect the greater response to PTH of IGF-I and IGF-IR gene expression in males enhancing the anabolic effect on cortical bone.

scholarly journals Mutation of the Galectin-3 Glycan Binding Domain (Lgals3-R200S) Enhances Cortical Bone Expansion in Male and Trabecular Bone Mass in Female Mice

2020 ◽  
Author(s):  
Kevin A. Maupin ◽  
Daniel Dick ◽  
VARI Vivarium ◽  
Transgenics Core ◽  
Bart O. Williams
Keyword(s):  
Bone Mass ◽  
Trabecular Bone ◽  
Cortical Bone ◽  
Mutant Mice ◽  
Slight Variation ◽  
Female Mice ◽  
Bone Phenotype ◽  
Bone Expansion ◽  
Galectin 3 ◽  
Trabecular Bone Mass

AbstractThe study of galectin-3 is complicated by its ability to function both intracellularly and extracellularly. While the mechanism of galectin-3 secretion is unclear, studies have shown that the mutation of a highly conserved arginine to a serine in human galectin-3 (LGALS3-R186S) blocks glycan binding and secretion. To gain insight into the roles of extracellular and intracellular functions of galectin-3, we generated mice with the equivalent mutation (Lgals3-R200S) using CRISPR/Cas9-directed homologous recombination. Consistent with a reduction in galectin-3 secretion, we observed significantly reduced galectin-3 protein levels in the plasma of heterozygous and homozygous mutant mice. We observed a similar increased bone mass phenotype in Lgals3-R200S mutant mice at 36 weeks as we previously observed in Lgals3-KO mice with slight variation. Like Lgals3-KO mice, Lgals3-R200S females, but not males, had significantly increased trabecular bone mass. However, only male Lgals3-R200S mice showed increased cortical bone expansion, which we had previously observed in both male and female Lgals3-KO mice and only in female mice using a separate Lgals3 null allele (Lgals3). These results suggest that the trabecular bone phenotype of Lgals3-KO mice was driven primarily by loss of extracellular galectin-3. However, the cortical bone phenotype of Lgals3-KO mice may have also been influenced by loss of intracellular galectin-3. Future analyses of these mice will aid in identifying the cellular and molecular mechanisms that contribute to the Lgals3-deficient bone phenotype as well as aid in distinguishing the extracellular vs. intracellular roles of galectin-3 in various signaling pathways.

scholarly journals A Surrogate Measure of Cortical Bone Matrix Density by Long T2-Suppressed MRI

2015 ◽  
Vol 30 (12) ◽  
pp. 2229-2238 ◽  
Author(s):  
Alan C Seifert ◽  
Cheng Li ◽  
Suzanne L Wehrli ◽  
Felix W Wehrli
Keyword(s):  
Cortical Bone ◽  
Bone Matrix ◽  
Matrix Density ◽  
Surrogate Measure

Osteochondral Defect Repair by Demineralized Cortical Bone Matrix

2004 ◽  
Vol 427 ◽  
pp. S62-S66 ◽  
Author(s):  
Jizong Gao ◽  
David Knaack ◽  
Victor M Goldberg ◽  
Arnold I Caplan
Keyword(s):  
Cortical Bone ◽  
Osteochondral Defect ◽  
Bone Matrix ◽  
Defect Repair

The use of decalcified granulated homologous cortical bone matrix in the correction of diaphyseal bone defect

1982 ◽  
Vol 99 (3) ◽  
pp. 199-207 ◽  
Author(s):  
Jos� B. Volpon ◽  
Camilo A. M. Xavier ◽  
Renato Pinto Con�alves
Keyword(s):  
Cortical Bone ◽  
Bone Defect ◽  
Bone Matrix ◽  
Diaphyseal Bone
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