A single 2-day pulse of activin-A leads to a transient change in gene expression eventually followed by reduction in extracellular matrix mineralization

2016 ◽  
Author(s):  
Marta Baroncelli ◽  
Ksenija Drabek ◽  
Marco Eijken ◽  
Jeroen van de Peppel ◽  
Leeuwen Johannes van
Keyword(s):  
Gene Expression ◽  
Extracellular Matrix ◽  
Activin A ◽  
Matrix Mineralization ◽  
Transient Change

scholarly journals Two‐day‐treatment of Activin‐A leads to transient change in SV‐HFO osteoblast gene expression and reduction in matrix mineralization

2019 ◽  
Vol 235 (5) ◽  
pp. 4865-4877 ◽  
Author(s):  
Marta Baroncelli ◽  
Ksenija Drabek ◽  
Marco Eijken ◽  
Bram C. J. Eerden ◽  
Jeroen Peppel ◽  
...  
Keyword(s):  
Gene Expression ◽  
Day Treatment ◽  
Activin A ◽  
Matrix Mineralization ◽  
Transient Change

Extracellular Matrix Mineralization and Osteoblast Gene Expression by Human Adipose Tissue–Derived Stromal Cells

2001 ◽  
Vol 7 (6) ◽  
pp. 729-741 ◽  
Author(s):  
Yuan-Di C. Halvorsen ◽  
Dawn Franklin ◽  
Arden L. Bond ◽  
Daron C. Hitt ◽  
Catherine Auchter ◽  
...  
Keyword(s):  
Gene Expression ◽  
Extracellular Matrix ◽  
Adipose Tissue ◽  
Stromal Cells ◽  
Human Adipose Tissue ◽  
Matrix Mineralization

The activin A‐follistatin system: potent regulator of human extracellular matrix mineralization

2007 ◽  
Vol 21 (11) ◽  
pp. 2949-2960 ◽  
Author(s):  
Marco Eijken ◽  
Sigrid Swagemakers ◽  
Marijke Koedam ◽  
Cobie Steenbergen ◽  
Pieter Derkx ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Activin A ◽  
Matrix Mineralization

Vitamin D Control of Osteoblast Function and Bone Extracellular Matrix Mineralization

2001 ◽  
Vol 11 (1-3) ◽  
pp. 28 ◽  
Author(s):  
J. P. T. M. van Leeuwen ◽  
M. van Driel ◽  
G. J. C. M. van den Bemd ◽  
H. A. P. Pols
Keyword(s):  
Vitamin D ◽  
Extracellular Matrix ◽  
Matrix Mineralization ◽  
Osteoblast Function

The Therapeutic Potential and Role of miRNA, lncRNA, and circRNA in Osteoarthritis

2019 ◽  
Vol 19 (4) ◽  
pp. 255-263 ◽  
Author(s):  
Yuangang Wu ◽  
Xiaoxi Lu ◽  
Bin Shen ◽  
Yi Zeng
Keyword(s):  
Gene Expression ◽  
Gene Therapy ◽  
Extracellular Matrix ◽  
Inflammatory Reaction ◽  
Noncoding Rna ◽  
Rna Binding ◽  
Rna Binding Proteins ◽  
Protein Translation ◽  
Cochrane Library

Background: Osteoarthritis (OA) is a disease characterized by progressive degeneration, joint hyperplasia, narrowing of joint spaces, and extracellular matrix metabolism. Recent studies have shown that the pathogenesis of OA may be related to non-coding RNA, and its pathological mechanism may be an effective way to reduce OA. Objective: The purpose of this review was to investigate the recent progress of miRNA, long noncoding RNA (lncRNA) and circular RNA (circRNA) in gene therapy of OA, discussing the effects of this RNA on gene expression, inflammatory reaction, apoptosis and extracellular matrix in OA. Methods: The following electronic databases were searched, including PubMed, EMBASE, Web of Science, and the Cochrane Library, for published studies involving the miRNA, lncRNA, and circRNA in OA. The outcomes included the gene expression, inflammatory reaction, apoptosis, and extracellular matrix. Results and Discussion: With the development of technology, miRNA, lncRNA, and circRNA have been found in many diseases. More importantly, recent studies have found that RNA interacts with RNA-binding proteins to regulate gene transcription and protein translation, and is involved in various pathological processes of OA, thus becoming a potential therapy for OA. Conclusion: In this paper, we briefly introduced the role of miRNA, lncRNA, and circRNA in the occurrence and development of OA and as a new target for gene therapy.

scholarly journals Expression of inter-alpha-trypsin inhibitor heavy chains in endometrium of cyclic and pregnant gilts

Reproduction ◽  
2003 ◽  
pp. 621-627 ◽  
Author(s):  
RD Geisert ◽  
MD Ashworth ◽  
Keyword(s):  
Gene Expression ◽  
Extracellular Matrix ◽  
Western Blot ◽  
Protease Inhibitor ◽  
Early Pregnancy ◽  
Trypsin Inhibitor ◽  
Serine Protease Inhibitor ◽  
Oestrous Cycle ◽  
Heavy Chains ◽  
Formation Of Complexes

Attachment of the placenta to the uterus in pigs involves extracellular interaction between the expanding trophoblastic membrane and the thick glycocalyx present on the uterine epithelial microvilli. Formation of complexes between members of inter-alpha-trypsin inhibitor family may function in the maintenance of the extracellular matrix. This study investigated the change in the inter-alpha-trypsin inhibitor heavy chains (ITIH1, ITIH2, ITIH3 and ITIH4) during the oestrous cycle and early pregnancy in pigs. Gene expression of ITIH1, ITIH2, ITIH3 and ITIH4 was detected in the endometrium of cyclic and pregnant gilts; however, gene expression of ITIH was not altered throughout the oestrous cycle or early pregnancy. Western blot analysis with an ITIH antiserum identified the possible linkage forms of ITIH with the serine protease inhibitor, bikunin. Pregnancy altered the release of the various inter-alpha-inhibitor forms from the endometrium during the period of trophoblastic attachment. The results from this study indicate that the inter-alpha-trypsin inhibitor family plays an important role in maintenance of the uterine surface glycocalyx during placental attachment in pigs.

Global gene expression profiles reveal an increase in mRNA levels of collagens, MMPs, and TIMPs in late radiation enteritis

2004 ◽  
Vol 287 (4) ◽  
pp. G875-G885 ◽  
Author(s):  
Carine Strup-Perrot ◽  
Denis Mathé ◽  
Christine Linard ◽  
Dominique Violot ◽  
Fabien Milliat ◽  
...  
Keyword(s):  
Gene Expression ◽  
Extracellular Matrix ◽  
Expression Profiles ◽  
Cdna Array ◽  
Muscularis Propria ◽  
Gelatin Zymography ◽  
Tissue Inhibitors Of Metalloproteinases ◽  
Radiation Enteritis ◽  
Mrna Levels ◽  
Fixed Tissue

Radiation enteritis, a common complication of radiation therapy for abdominal and pelvic cancers, is characterized by severe transmural fibrosis associated with mesenchymal cell activation, tissue disorganization, and deposition of fibrillar collagen. To investigate the mechanisms involved in this pathological accumulation of extracellular matrix, we studied gene expression of matrix components along with that of genes involved in matrix remodeling, matrix metalloproteinases (MMPs), and tissue inhibitors of metalloproteinases (TIMPs). Hybrid selection on high-density cDNA array, real-time RT-PCR, gelatin zymography and imunohistochemistry were used to characterize the mRNA expression profile, activity, and tissue location of extracellular matrix-related genes in radiation enteritis compared with healthy ileum. cDNA array analysis revealed a strong induction of genes coding for collagens I, III, IV, VI, and VIII, SPARC, and tenascin-C, extracellular-matrix degrading enzymes (MMP-1, -2, -3, -14, -18+19), and metalloproteinase inhibitors (TIMP-1, -2, plasminogen activator inhibitor-1) in radiation enteritis. This increase was correlated with the degree of infiltration of the mucosa by inflammatory cells, and the presence of differentiated mesenchymal cells in the submucosa and muscularis propria. Despite the fact that expression of collagens, MMPs, and TIMPs simultaneously increase, quantification of net collagen deposition shows an overall accumulation of collagen. Our results indicate that late radiation enteritis tissues are subjected to active process of fibrogenesis as well as fibrolysis, with a balance toward fibrogenesis. This demonstrates that established fibrotic tissue is not scarred fixed tissue but is subjected to a dynamic remodeling process.

scholarly journals Thromboxane and prostacyclin differentially regulate murine extracellular matrix gene expression

1993 ◽  
Vol 43 (6) ◽  
pp. 1219-1225 ◽  
Author(s):  
Leslie A. Bruggeman ◽  
Jill A. Pellicoro ◽  
Elizabeth A. Horigan ◽  
Paul E. Klotman
Keyword(s):  
Gene Expression ◽  
Extracellular Matrix ◽  
Matrix Gene
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