The activin A‐follistatin system: potent regulator of human extracellular matrix mineralization

2007 ◽  
Vol 21 (11) ◽  
pp. 2949-2960 ◽  
Author(s):  
Marco Eijken ◽  
Sigrid Swagemakers ◽  
Marijke Koedam ◽  
Cobie Steenbergen ◽  
Pieter Derkx ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Activin A ◽  
Matrix Mineralization

Vitamin D Control of Osteoblast Function and Bone Extracellular Matrix Mineralization

2001 ◽  
Vol 11 (1-3) ◽  
pp. 28 ◽  
Author(s):  
J. P. T. M. van Leeuwen ◽  
M. van Driel ◽  
G. J. C. M. van den Bemd ◽  
H. A. P. Pols
Keyword(s):  
Vitamin D ◽  
Extracellular Matrix ◽  
Matrix Mineralization ◽  
Osteoblast Function

scholarly journals Effect of dexamethasone as osteogenic supplementation in in vitro osteogenic differentiation of stem cells from human exfoliated deciduous teeth

2021 ◽  
Vol 32 (1) ◽  
Author(s):  
Mariane Beatriz Sordi ◽  
Raissa Borges Curtarelli ◽  
Izabella Thaís da Silva ◽  
Gislaine Fongaro ◽  
Cesar Augusto Magalhães Benfatti ◽  
...  
Keyword(s):  
Stem Cells ◽  
Extracellular Matrix ◽  
Osteogenic Differentiation ◽  
Culture Media ◽  
Deciduous Teeth ◽  
Free Calcium ◽  
Alizarin Red ◽  
Matrix Mineralization ◽  
Media Supplementation

AbstractIn in vitro culture systems, dexamethasone (DEX) has been applied with ascorbic acid (ASC) and β-glycerophosphate (βGLY) as culture media supplementation to induce osteogenic differentiation of mesenchymal stem cells. However, there are some inconsistencies regarding the role of DEX as osteogenic media supplementation. Therefore, this study verified the influence of DEX culture media supplementation on the osteogenic differentiation, especially the capacity to mineralize the extracellular matrix of stem cells from human exfoliated deciduous teeth (SHED). Five groups were established: G1—SHED + Dulbecco’s Modified Eagles’ Medium (DMEM) + fetal bovine serum (FBS); G2—SHED + DMEM + FBS + DEX; G3—SHED + DMEM + FBS + ASC + βGLY; G4—SHED + DMEM + FBS + ASC + βGLY + DEX; G5—MC3T3-E1 + α Minimal Essential Medium (MEM) + FBS + ASC + βGLY. DNA content, alkaline phosphatase (ALP) activity, free calcium quantification in the extracellular medium, and extracellular matrix mineralization quantification through staining with von Kossa, alizarin red, and tetracycline were performed on days 7 and 21. Osteogenic media supplemented with ASC and β-GLY demonstrated similar effects on SHED in the presence or absence of DEX for DNA content (day 21) and capacity to mineralize the extracellular matrix according to alizarin red and tetracycline quantifications (day 21). In addition, the presence of DEX in the osteogenic medium promoted less ALP activity (day 7) and extracellular matrix mineralization according to the von Kossa assay (day 21), and more free calcium quantification at extracellular medium (day 21). In summary, the presence of DEX in the osteogenic media supplementation did not interfere with SHED commitment into mineral matrix depositor cells. We suggest that DEX may be omitted from culture media supplementation for SHED osteogenic differentiation in vitro studies.

scholarly journals Polyphosphates inhibit extracellular matrix mineralization in MC3T3-E1 osteoblast cultures

Bone ◽  
2013 ◽  
Vol 53 (2) ◽  
pp. 478-486 ◽  
Author(s):  
Betty Hoac ◽  
Tina Kiffer-Moreira ◽  
José Luis Millán ◽  
Marc D. McKee
Keyword(s):  
Extracellular Matrix ◽  
Matrix Mineralization

scholarly journals Two‐day‐treatment of Activin‐A leads to transient change in SV‐HFO osteoblast gene expression and reduction in matrix mineralization

2019 ◽  
Vol 235 (5) ◽  
pp. 4865-4877 ◽  
Author(s):  
Marta Baroncelli ◽  
Ksenija Drabek ◽  
Marco Eijken ◽  
Bram C. J. Eerden ◽  
Jeroen Peppel ◽  
...  
Keyword(s):  
Gene Expression ◽  
Day Treatment ◽  
Activin A ◽  
Matrix Mineralization ◽  
Transient Change
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