High-resolution molecular validation of self-renewal and spontaneous differentiation in adipose-tissue derived human mesenchymal stem cells cultured in human platelet lysate

2014 ◽  
Author(s):  
Amel Dudakovic ◽  
Emily Camilleri ◽  
Scott Riester ◽  
Jennifer Westendorf ◽  
Sanjeev Kakar ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
High Resolution ◽  
Human Platelet ◽  
Human Mesenchymal Stem Cells ◽  
Platelet Lysate ◽  
Self Renewal ◽  
Human Platelet Lysate ◽  
Cells Cultured

scholarly journals 3D gelatin-chitosan hybrid hydrogels combined with human platelet lysate highly support human mesenchymal stem cell proliferation and osteogenic differentiation

2019 ◽  
Vol 10 ◽  
pp. 204173141984585 ◽  
Author(s):  
Federica Re ◽  
Luciana Sartore ◽  
Vladimira Moulisova ◽  
Marco Cantini ◽  
Camillo Almici ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Human Platelet ◽  
Human Mesenchymal Stem Cells ◽  
Platelet Lysate ◽  
Hybrid Hydrogel ◽  
Hybrid Hydrogels ◽  
Human Platelet Lysate

Bone marrow and adipose tissue human mesenchymal stem cells were seeded in highly performing 3D gelatin–chitosan hybrid hydrogels of varying chitosan content in the presence of human platelet lysate and evaluated for their proliferation and osteogenic differentiation. Both bone marrow and adipose tissue human mesenchymal stem cells in gelatin–chitosan hybrid hydrogel 1 (chitosan content 8.1%) or gelatin–chitosan hybrid hydrogel 2 (chitosan 14.9%) showed high levels of viability (80%–90%), and their proliferation and osteogenic differentiation was significantly higher with human platelet lysate compared to fetal bovine serum, particularly in gelatin–chitosan hybrid hydrogel 1. Mineralization was detected early, after 21 days of culture, when human platelet lysate was used in the presence of osteogenic stimuli. Proteomic characterization of human platelet lysate highlighted 59 proteins mainly involved in functions related to cell adhesion, cellular repairing mechanisms, and regulation of cell differentiation. In conclusion, the combination of our gelatin–chitosan hybrid hydrogels with hPL represents a promising strategy for bone regenerative medicine using human mesenchymal stem cells.

Immunomodulative Efficacy of Bone Marrow-Derived Mesenchymal Stem Cells Cultured in Human Platelet Lysate

2011 ◽  
Vol 31 (6) ◽  
pp. 1143-1156 ◽  
Author(s):  
Antoinette Flemming ◽  
Katharina Schallmoser ◽  
Dirk Strunk ◽  
Meaghan Stolk ◽  
Hans-Dieter Volk ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Human Platelet ◽  
Platelet Lysate ◽  
Human Platelet Lysate ◽  
Cells Cultured
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