IGF1 regulates MC-3T3 and human primary osteoblast to osteocyte differentiation in 3D culture

2013 ◽  
Author(s):  
Nicole E E Scully ◽  
Deborah J Mason ◽  
Bronwen A J Evans
Keyword(s):  
3D Culture ◽  
Primary Osteoblast ◽  
Human Primary Osteoblast

Effect of 1α,25(OH)2-Vitamin D3on TNFα-Mediated Apoptosis of Human Primary Osteoblast-Like Cellsin Vitro

1999 ◽  
Vol 31 (12) ◽  
pp. 653-656 ◽  
Author(s):  
E. Pascher ◽  
A. Perniok ◽  
A. Becker ◽  
J. Feldkamp
Keyword(s):  
Primary Osteoblast ◽  
Human Primary Osteoblast

L-Carnitine facilitates mitochondrial activity and osteogenic differentiation in human primary osteoblast culture

2016 ◽  
Author(s):  
Fernanda Vacante ◽  
Anna Montesano ◽  
Alice Spinello ◽  
Simona Bolamperti ◽  
Pamela Senesi ◽  
...  
Keyword(s):  
Osteogenic Differentiation ◽  
Mitochondrial Activity ◽  
Primary Osteoblast ◽  
Osteoblast Culture ◽  
Human Primary Osteoblast

Mineralized human primary osteoblast matrices as a model system to analyse interactions of prostate cancer cells with the bone microenvironment

Biomaterials ◽  
2010 ◽  
Vol 31 (31) ◽  
pp. 7928-7936 ◽  
Author(s):  
Johannes C. Reichert ◽  
Verena M.C. Quent ◽  
Leslie J. Burke ◽  
Scott H. Stansfield ◽  
Judith A. Clements ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cancer Cells ◽  
Model System ◽  
Bone Microenvironment ◽  
Prostate Cancer Cells ◽  
Primary Osteoblast ◽  
Human Primary Osteoblast

Casein phosphopeptides promote calcium uptake and modulate the differentiation pathway in human primary osteoblast-like cells

Peptides ◽  
2009 ◽  
Vol 30 (12) ◽  
pp. 2233-2241 ◽  
Author(s):  
Bianca Maria Donida ◽  
Emanuela Mrak ◽  
Claudia Gravaghi ◽  
Isabella Villa ◽  
Stefania Cosentino ◽  
...  
Keyword(s):  
Calcium Uptake ◽  
Primary Osteoblast ◽  
Casein Phosphopeptides ◽  
Human Primary Osteoblast

scholarly journals The Degradation Interface of Magnesium Based Alloys in Direct Contact with Human Primary Osteoblast Cells

PLoS ONE ◽  
2016 ◽  
Vol 11 (6) ◽  
pp. e0157874 ◽  
Author(s):  
Nezha Ahmad Agha ◽  
Regine Willumeit-Römer ◽  
Daniel Laipple ◽  
Bérengère Luthringer ◽  
Frank Feyerabend
Keyword(s):  
Direct Contact ◽  
Osteoblast Cells ◽  
Primary Osteoblast ◽  
Human Primary Osteoblast

scholarly journals Biomimetic Mineralization on 3D Printed PLA Scaffolds: On the Response of Human Primary Osteoblasts Spheroids and In Vivo Implantation

Polymers ◽  
2020 ◽  
Vol 13 (1) ◽  
pp. 74
Author(s):  
Marianna O. C. Maia-Pinto ◽  
Ana Carolina B. Brochado ◽  
Bruna Nunes Teixeira ◽  
Suelen C. Sartoretto ◽  
Marcelo J. Uzeda ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Growth Factors ◽  
Growth Factor ◽  
Biological Response ◽  
Primary Osteoblast ◽  
Rat Calvaria ◽  
3D Printed ◽  
Human Primary Osteoblast

This study aimed to assess the response of 3D printed polylactic acid (PLA) scaffolds biomimetically coated with apatite on human primary osteoblast (HOb) spheroids and evaluate the biological response to its association with Bone Morphogenetic Protein 2 (rhBMP-2) in rat calvaria. PLA scaffolds were produced via 3D printing, soaked in simulated body fluid (SBF) solution to promote apatite deposition, and characterized by physical-chemical, morphological, and mechanical properties. PLA-CaP scaffolds with interconnected porous and mechanical properties suitable for bone repairing were produced with reproducibility. The in vitro biological response was assessed with human primary osteoblast spheroids. Increased cell adhesion and the rise of in vitro release of growth factors (Platelet-Derived Growth Factor (PDGF), Basic Fibroblast Growth Factor (bFGF), Vascular Endothelial Growth Factor (VEGF) was observed for PLA-CaP scaffolds, when pre-treated with fetal bovine serum (FBS). This pre-treatment with FBS was done in a way to enhance the adsorption of serum proteins, increasing the number of bioactive sites on the surface of scaffolds, and to partially mimic in vivo interactions. The in vivo analysis was conducted through the implantation of 3D printed PLA scaffolds either alone, coated with apatite (PLA-CaP) or PLA-CaP loaded with rhBMP-2 on critical-sized defects (8 mm) of rat calvaria. PLA-CaP+rhBMP2 presented higher values of newly formed bone (NFB) than other groups at all in vivo experimental periods (p < 0.05), attaining 44.85% of NFB after six months. These findings indicated two new potential candidates as alternatives to autogenous bone grafts for long-term treatment: (i) 3D-printed PLA-CaP scaffold associated with spheroids, since it can reduce the time of repair in situ by expression of biomolecules and growth factors; and (ii) 3D-printed PLA-CaP functionalized rhBMP2 scaffold, a biocompatible, bioactive biomaterial, with osteoconductivity and osteoinductivity.

scholarly journals BMP-4 of Xenopus laevis stimulates differentiation of human primary osteoblast-like cells

2001 ◽  
Vol 83-B (1) ◽  
pp. 144-147
Author(s):  
U. Mayr-Wohlfart ◽  
S. Kessler ◽  
W. Puhl ◽  
K. P. Günther ◽  
W. Knöchel
Keyword(s):  
Xenopus Laevis ◽  
Primary Osteoblast ◽  
Human Primary Osteoblast

scholarly journals The biocompatibility of silver and nanohydroxyapatite coatings on titanium dental implants with human primary osteoblast cells

2020 ◽  
Vol 107 ◽  
pp. 110210 ◽  
Author(s):  
Ranj Nadhim Salaie ◽  
Alexandros Besinis ◽  
Huirong Le ◽  
Christopher Tredwin ◽  
Richard D. Handy
Keyword(s):  
Dental Implants ◽  
Osteoblast Cells ◽  
Primary Osteoblast ◽  
Titanium Dental Implants ◽  
Human Primary Osteoblast
Sign in / Sign up

Export Citation Format

Share Document