L-Carnitine facilitates mitochondrial activity and osteogenic differentiation in human primary osteoblast culture

2016 ◽  
Author(s):  
Fernanda Vacante ◽  
Anna Montesano ◽  
Alice Spinello ◽  
Simona Bolamperti ◽  
Pamela Senesi ◽  
...  
Keyword(s):  
Osteogenic Differentiation ◽  
Mitochondrial Activity ◽  
Primary Osteoblast ◽  
Osteoblast Culture ◽  
Human Primary Osteoblast

Primary osteoblast culture from red fox (Vulpes Vulpes)

2016 ◽  
Author(s):  
Ines Pedro Perpetuo ◽  
Alessandro Felder ◽  
Andrew Pitsillides ◽  
Michael Doube ◽  
Isabel Orriss
Keyword(s):  
Vulpes Vulpes ◽  
Red Fox ◽  
Primary Osteoblast ◽  
Osteoblast Culture

Primary osteoblast culture from domestic dog (Canis lupus familiaris)

2016 ◽  
Author(s):  
Ines Pedro Perpetuo ◽  
Richard Meeson ◽  
Andrew Pitsillides ◽  
Michael Doube ◽  
Isabel Orriss
Keyword(s):  
Canis Lupus ◽  
Domestic Dog ◽  
Canis Lupus Familiaris ◽  
Primary Osteoblast ◽  
Osteoblast Culture

scholarly journals The Effect of Various Surface Treatments of Ti6Al4V on the Growth and Osteogenic Differentiation of Adipose Tissue-Derived Stem Cells

Coatings ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 762
Author(s):  
Jana Stepanovska ◽  
Roman Matejka ◽  
Martin Otahal ◽  
Jozef Rosina ◽  
Lucie Bacakova
Keyword(s):  
Stem Cells ◽  
Surface Roughness ◽  
Adipose Tissue ◽  
Osteogenic Differentiation ◽  
Water Drop ◽  
Chemical Properties ◽  
Surface Modifications ◽  
Cell Number ◽  
Material Surface ◽  
Mitochondrial Activity

The physical and chemical properties of the material surface, especially its roughness and wettability, have a crucial effect on the adhesion, proliferation, and differentiation of cells. The aim of this study is to select the most appropriate surface modifications of Ti6Al4V implants for pre-colonization of the implants with adipose tissue-derived stem cells (ASCs) in order to improve their osseointegration. We compared the adhesion, growth, and osteogenic differentiation of rat ASCs on Ti6Al4V samples modified by methods commonly used for preparing clinically used titanium-based implants, namely polishing (PL), coating with diamond-like carbon (DLC), brushing (BR), anodizing (AND), and blasting (BL). The material surface roughness, measured by the Ra and Rq parameters, increased in the following order: PL < DLC ˂ BR ˂ AND ˂ BL. The water drop contact angle was in the range of 60–74°, with the exception of the DLC-coated samples, where it was only 38°. The cell number, morphology, mitochondrial activity, relative fluorescence intensity of osteogenic markers RUNX2, type 1 collagen, and osteopontin, the calcium consumption by the cells and the alkaline phosphatase activity depended on the surface roughness rather than on the surface wettability of the materials. Materials with a surface roughness of several tens of nanometers (Ra 60–70 nm), i.e., the BR and AND samples, supported a satisfactory level of cell proliferation. At the same time, they achieved the highest level of osteogenic cell differentiation. These surface modifications therefore seem to be most suitable for pre-colonization of Ti6Al4V implants with stem cells pre-differentiated toward osteoblasts, and then for implanting them into the bone tissue.

Effect of 1α,25(OH)2-Vitamin D3on TNFα-Mediated Apoptosis of Human Primary Osteoblast-Like Cellsin Vitro

1999 ◽  
Vol 31 (12) ◽  
pp. 653-656 ◽  
Author(s):  
E. Pascher ◽  
A. Perniok ◽  
A. Becker ◽  
J. Feldkamp
Keyword(s):  
Primary Osteoblast ◽  
Human Primary Osteoblast

Biodegradable polymer promotes osteogenic differentiation in immortalized and primary osteoblast-like cells

2019 ◽  
Vol 14 (4) ◽  
pp. 045003 ◽  
Author(s):  
Bora Onat ◽  
Sinem Tunçer ◽  
Sinem Ulusan ◽  
Sreeparna Banerjee ◽  
Irem Erel-Göktepe
Keyword(s):  
Osteogenic Differentiation ◽  
Biodegradable Polymer ◽  
Primary Osteoblast

Mineralized human primary osteoblast matrices as a model system to analyse interactions of prostate cancer cells with the bone microenvironment

Biomaterials ◽  
2010 ◽  
Vol 31 (31) ◽  
pp. 7928-7936 ◽  
Author(s):  
Johannes C. Reichert ◽  
Verena M.C. Quent ◽  
Leslie J. Burke ◽  
Scott H. Stansfield ◽  
Judith A. Clements ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cancer Cells ◽  
Model System ◽  
Bone Microenvironment ◽  
Prostate Cancer Cells ◽  
Primary Osteoblast ◽  
Human Primary Osteoblast

Casein phosphopeptides promote calcium uptake and modulate the differentiation pathway in human primary osteoblast-like cells

Peptides ◽  
2009 ◽  
Vol 30 (12) ◽  
pp. 2233-2241 ◽  
Author(s):  
Bianca Maria Donida ◽  
Emanuela Mrak ◽  
Claudia Gravaghi ◽  
Isabella Villa ◽  
Stefania Cosentino ◽  
...  
Keyword(s):  
Calcium Uptake ◽  
Primary Osteoblast ◽  
Casein Phosphopeptides ◽  
Human Primary Osteoblast

scholarly journals The Degradation Interface of Magnesium Based Alloys in Direct Contact with Human Primary Osteoblast Cells

PLoS ONE ◽  
2016 ◽  
Vol 11 (6) ◽  
pp. e0157874 ◽  
Author(s):  
Nezha Ahmad Agha ◽  
Regine Willumeit-Römer ◽  
Daniel Laipple ◽  
Bérengère Luthringer ◽  
Frank Feyerabend
Keyword(s):  
Direct Contact ◽  
Osteoblast Cells ◽  
Primary Osteoblast ◽  
Human Primary Osteoblast

scholarly journals Biomimetic Mineralization on 3D Printed PLA Scaffolds: On the Response of Human Primary Osteoblasts Spheroids and In Vivo Implantation

Polymers ◽  
2020 ◽  
Vol 13 (1) ◽  
pp. 74
Author(s):  
Marianna O. C. Maia-Pinto ◽  
Ana Carolina B. Brochado ◽  
Bruna Nunes Teixeira ◽  
Suelen C. Sartoretto ◽  
Marcelo J. Uzeda ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Growth Factors ◽  
Growth Factor ◽  
Biological Response ◽  
Primary Osteoblast ◽  
Rat Calvaria ◽  
3D Printed ◽  
Human Primary Osteoblast

This study aimed to assess the response of 3D printed polylactic acid (PLA) scaffolds biomimetically coated with apatite on human primary osteoblast (HOb) spheroids and evaluate the biological response to its association with Bone Morphogenetic Protein 2 (rhBMP-2) in rat calvaria. PLA scaffolds were produced via 3D printing, soaked in simulated body fluid (SBF) solution to promote apatite deposition, and characterized by physical-chemical, morphological, and mechanical properties. PLA-CaP scaffolds with interconnected porous and mechanical properties suitable for bone repairing were produced with reproducibility. The in vitro biological response was assessed with human primary osteoblast spheroids. Increased cell adhesion and the rise of in vitro release of growth factors (Platelet-Derived Growth Factor (PDGF), Basic Fibroblast Growth Factor (bFGF), Vascular Endothelial Growth Factor (VEGF) was observed for PLA-CaP scaffolds, when pre-treated with fetal bovine serum (FBS). This pre-treatment with FBS was done in a way to enhance the adsorption of serum proteins, increasing the number of bioactive sites on the surface of scaffolds, and to partially mimic in vivo interactions. The in vivo analysis was conducted through the implantation of 3D printed PLA scaffolds either alone, coated with apatite (PLA-CaP) or PLA-CaP loaded with rhBMP-2 on critical-sized defects (8 mm) of rat calvaria. PLA-CaP+rhBMP2 presented higher values of newly formed bone (NFB) than other groups at all in vivo experimental periods (p < 0.05), attaining 44.85% of NFB after six months. These findings indicated two new potential candidates as alternatives to autogenous bone grafts for long-term treatment: (i) 3D-printed PLA-CaP scaffold associated with spheroids, since it can reduce the time of repair in situ by expression of biomolecules and growth factors; and (ii) 3D-printed PLA-CaP functionalized rhBMP2 scaffold, a biocompatible, bioactive biomaterial, with osteoconductivity and osteoinductivity.

Sign in / Sign up

Export Citation Format

Share Document