Moderate hypothermia induces growth arrest in normal human osteoblast cells but retained mitochondrial metabolism in vitro

2013 ◽  
Author(s):  
Mohd Din Aisha ◽  
Mohamed Noor Khan Nor-Ashikin ◽  
Ab. Rahim Sharaniza ◽  
Hapizah Nawawi ◽  
Marina Kapitonova ◽  
...  
Keyword(s):  
Growth Arrest ◽  
Mitochondrial Metabolism ◽  
Moderate Hypothermia ◽  
Human Osteoblast ◽  
Osteoblast Cells ◽  
Normal Human ◽  
Normal Human Osteoblast

Adhesion and Cell Viability of Normal Human Osteoblasts (NHOst) on Scaffolds of Poly (3-hydroxybutyrate)

2015 ◽  
Vol 1721 ◽  
Author(s):  
Maraolina Domínguez-Díaz ◽  
Angelica Meneses-Acosta ◽  
Angel Romo-Uribe
Keyword(s):  
Contact Angle ◽  
Cell Adhesion ◽  
Cell Viability ◽  
Human Osteoblast ◽  
Cell Nuclei ◽  
Electrospun Membrane ◽  
Osteoblast Cells ◽  
Cast Film ◽  
Normal Human ◽  
Normal Human Osteoblast

ABSTRACTBiodegradable Normal Human Osteoblast (NHOst) cells were inoculated into the polymer scaffolds of poly(β-hydroxybutyrate) (PHB) obtained from a specially developed strain of Azotobacter vinelandii. Cell adhesion is essential to promote growth on scaffolds for tissue engineering. Thus, in this research we focused on the adhesion of osteoblast cells to PHB scaffolds produced by solution casting and electrospinning. Cell viability was also investigated up to 168 hrs. Water contact angle on the PHB scaffolds was determined prior to the cells inoculation. The contact angle is usually related to the ability of different cell strains to adhere to a given material. The as cast film exhibited a contact angle α=72° whereas for the electrospun membrane α=102°, thus in theory cell adhesion would be greater for the cast film. Biological testing was carried out on plates of 24 wells; cell viability was determined by Trypan Blue, cell morphology by optical microscopy, and cell nuclei integrity by staining with Acridine orange. Parallel studies were carried out on control (empty) wells. Microscopy observations 168 hrs after cell inoculation showed larger quantities of osteoblast cells in the wells containing PHB scaffolds and the cell nuclei were still active. Moreover, it was found that the cells grew inside the PHB scaffolds and the cell viability was slightly greater for the electrospun scaffold. Interestingly, the time to remove the cells from the scaffolds (film and membranes) was increasing function of the cell culture time, therefore suggesting that PHB promotes adhesion of Normal Human Osteoblast cells to its surface.

Growth of Normal Human Osteoblast Cells on Hydroxyapatite/Titanium Alloy Composites Formed by Implantation of Hydroxyapatite Granules into Titanium Alloy

2005 ◽  
Vol 284-286 ◽  
pp. 843-846 ◽  
Author(s):  
Akira Watazu ◽  
H. Oguchi ◽  
T. Yamada ◽  
Tsutomu Sonoda ◽  
Kiyotaka Katou ◽  
...  
Keyword(s):  
Titanium Alloy ◽  
Human Osteoblast ◽  
Alloy Composite ◽  
Osteoblast Cells ◽  
Normal Human ◽  
Good Biocompatibility ◽  
Normal Human Osteoblast

On the hydroxyapatite/titanium alloy composite disks, the normal human osteoblast cells were cultured. As a result, the samples were covered with normal human osteoblast cells after 2 hours. Moreover, we did not observe cracks in any of the HA granules, and the normal human osteoblast cells extended between the HA granules and the titanium alloy. From these results, we have concluded that this material has good biocompatibility.

scholarly journals Osteoblast adhesion and proliferation on porous chitosan/polycaprolactone scaffolds for bone tissue engineering application

2019 ◽  
Vol 14 (4) ◽  
pp. 495-499
Author(s):  
Rashid Mad Jin ◽  
Naznin Sultana
Keyword(s):  
Tissue Engineering ◽  
Bone Graft ◽  
Bone Healing ◽  
Tissue Engineering Application ◽  
Human Osteoblast ◽  
Osteoblast Cells ◽  
Porous Chitosan ◽  
Proliferation And Differentiation ◽  
Normal Human ◽  
Normal Human Osteoblast

Tissue engineering is an evolving technique to reduce the limitations of the bone graft. It provides diversity to improve the healing process of bone fractures and defect by combining the use of 3D scaffolds, healing promoting factors, gene therapy and different drugs. Flexibility to use a different technique to fabricate scaffolds lead to the new insight into bone healing future. A bone graft is defined as promoted bone healing through osteogenesis, osteoinduction, and osteoconduction by the implanted material alone or with other materials. Ideal bone graft depends on several factors such as biomechanical characteristic, tissue viability, the morphological structure as well as biological characteristics. In this work, we have investigated adhesion, proliferation, and differentiation of poly (ɛ-caprolactone) / chitosan scaffolds with the incorporation of hydroxyapatite and tetracycline HCl on normal human osteoblast cells. Both of the polymers were blended without a cross-linking agent to form porous scaffolds by freeze-drying technique. From the results, it was observed that the compressive modulus increased from 4.0 MPa to 12.5 MPa and the yield strength increased from 0.48 MPa to 0.75 MPa for the PCL/CS scaffold and nHA/PCL/CS scaffold, respectively. Scanning electron microscopy study revealed that the cells successfully adhered to the surface of scaffolds after 24 hours incubation. Proliferation analysis exhibited the increasing trend of growth of cells. This study indicated that the scaffold fabricated using this technique was able to promote adhesion, proliferation, and differentiation of normal human osteoblast cells. 

Growth hormone stimulates proliferation and differentiation of normal human osteoblast-like cells in vitro

1993 ◽  
Vol 52 (3) ◽  
pp. 222-226 ◽  
Author(s):  
M. Kassem ◽  
W. Blum ◽  
J. Ristelli ◽  
L. Mosekilde ◽  
E. F. Eriksen
Keyword(s):  
Growth Hormone ◽  
Human Osteoblast ◽  
Proliferation And Differentiation ◽  
Normal Human ◽  
Normal Human Osteoblast

Cytotoxicity evaluation of biodegradable Zn–3Mg alloy toward normal human osteoblast cells

2015 ◽  
Vol 49 ◽  
pp. 560-566 ◽  
Author(s):  
N.S. Murni ◽  
M.S. Dambatta ◽  
S.K. Yeap ◽  
G.R.A. Froemming ◽  
H. Hermawan
Keyword(s):  
Human Osteoblast ◽  
Osteoblast Cells ◽  
Normal Human ◽  
Normal Human Osteoblast
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