Growth hormone stimulates proliferation and differentiation of normal human osteoblast-like cells in vitro

1993 ◽  
Vol 52 (3) ◽  
pp. 222-226 ◽  
Author(s):  
M. Kassem ◽  
W. Blum ◽  
J. Ristelli ◽  
L. Mosekilde ◽  
E. F. Eriksen
Keyword(s):  
Growth Hormone ◽  
Human Osteoblast ◽  
Proliferation And Differentiation ◽  
Normal Human ◽  
Normal Human Osteoblast

Osteoblast Differentiation and Apatite Formation on Gamma-Irradiated PLLA Sheets

2005 ◽  
Vol 288-289 ◽  
pp. 409-412
Author(s):  
Kazuo Isama ◽  
Toshie Tsuchiya
Keyword(s):  
Osteoblast Differentiation ◽  
Irradiation Dose ◽  
Apatite Formation ◽  
Γ Irradiation ◽  
Proliferation And Differentiation ◽  
Normal Human ◽  
Mouse Osteoblast ◽  
Gamma Irradiated ◽  
Normal Human Osteoblast

The effects of the γ-irradiatted PLLA on the osteoblasts and apatite formation were investigated in vitro. The PLLA sheet was γ-ray irradiated at the dose of 10, 25 or 50 kGy. The mouse osteoblast-like MC3T3-E1 cells and normal human osteoblast NHOst cells were micromass cultured on the PLLA sheet for 2 weeks, and then the proliferation and differentiation of the cells were determined. The proliferations of MC3T3-E1 and NHOst cells hardly changed with increasing irradiation dose. However, the differentiations of MC3T3-E1 and NHOst cells increased with irradiation dose. On the other hand, the surface of the PLLA sheet after soaking in the medium without the cells was characterized by SEM, EDX, FT-IR and XPS. The hydroxyapatite was formed on the surface of the PLLA sheet after soaking, and the amount of hydroxyapatite increased with irradiation dose. In summary, the γ-irradiated PLLA increased the differentiation of osteoblasts and also increased apatite-forming ability even without the osteoblasts. The osteoblast differentiation was enhanced well in the apatite formation on the surface of PLLA after the γ-irradiation.

scholarly journals Osteoblast adhesion and proliferation on porous chitosan/polycaprolactone scaffolds for bone tissue engineering application

2019 ◽  
Vol 14 (4) ◽  
pp. 495-499
Author(s):  
Rashid Mad Jin ◽  
Naznin Sultana
Keyword(s):  
Tissue Engineering ◽  
Bone Graft ◽  
Bone Healing ◽  
Tissue Engineering Application ◽  
Human Osteoblast ◽  
Osteoblast Cells ◽  
Porous Chitosan ◽  
Proliferation And Differentiation ◽  
Normal Human ◽  
Normal Human Osteoblast

Tissue engineering is an evolving technique to reduce the limitations of the bone graft. It provides diversity to improve the healing process of bone fractures and defect by combining the use of 3D scaffolds, healing promoting factors, gene therapy and different drugs. Flexibility to use a different technique to fabricate scaffolds lead to the new insight into bone healing future. A bone graft is defined as promoted bone healing through osteogenesis, osteoinduction, and osteoconduction by the implanted material alone or with other materials. Ideal bone graft depends on several factors such as biomechanical characteristic, tissue viability, the morphological structure as well as biological characteristics. In this work, we have investigated adhesion, proliferation, and differentiation of poly (ɛ-caprolactone) / chitosan scaffolds with the incorporation of hydroxyapatite and tetracycline HCl on normal human osteoblast cells. Both of the polymers were blended without a cross-linking agent to form porous scaffolds by freeze-drying technique. From the results, it was observed that the compressive modulus increased from 4.0 MPa to 12.5 MPa and the yield strength increased from 0.48 MPa to 0.75 MPa for the PCL/CS scaffold and nHA/PCL/CS scaffold, respectively. Scanning electron microscopy study revealed that the cells successfully adhered to the surface of scaffolds after 24 hours incubation. Proliferation analysis exhibited the increasing trend of growth of cells. This study indicated that the scaffold fabricated using this technique was able to promote adhesion, proliferation, and differentiation of normal human osteoblast cells. 

Lack of a direct effect of estrogen on proliferation and differentiation of normal human osteoblast-like cells

2009 ◽  
Vol 6 (3) ◽  
pp. 297-304 ◽  
Author(s):  
Philip E. Keeting ◽  
Robert E. Scott ◽  
Douglas S. Colvard ◽  
In K. Han ◽  
Thomas C. Spelsberg ◽  
...  
Keyword(s):  
Direct Effect ◽  
Human Osteoblast ◽  
Proliferation And Differentiation ◽  
Normal Human ◽  
Normal Human Osteoblast

Moderate hypothermia induces growth arrest in normal human osteoblast cells but retained mitochondrial metabolism in vitro

2013 ◽  
Author(s):  
Mohd Din Aisha ◽  
Mohamed Noor Khan Nor-Ashikin ◽  
Ab. Rahim Sharaniza ◽  
Hapizah Nawawi ◽  
Marina Kapitonova ◽  
...  
Keyword(s):  
Growth Arrest ◽  
Mitochondrial Metabolism ◽  
Moderate Hypothermia ◽  
Human Osteoblast ◽  
Osteoblast Cells ◽  
Normal Human ◽  
Normal Human Osteoblast

Somatomedin activity measured as sulphation factor in culture media from normal human liver and connective tissues explants. Effects of human growth hormone

1981 ◽  
Vol 98 (1) ◽  
pp. 24-28 ◽  
Author(s):  
R. M. Schimpff ◽  
M. Donnadieu ◽  
M. Gautier ◽  
G. Polini ◽  
A. M. Repellin
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Culture Media ◽  
Human Growth ◽  
Connective Tissues ◽  
Normal Human Liver ◽  
Normal Human ◽  
Higher Doses ◽  
Human Explants

Abstract. Normal human explants from liver and from connective tissues (aponeurosis or skin) incubated in vitro released sulphation activity measurable in chick embryo cartilage. Addition of human Growth Hormone (hGH) at physiological levels (10 ng/ml) increased the sulphation activity after 6 hours incubation time. Higher doses failed to increase the sulphation activity produced by connective tissues and decreased the sulphation activity produced by the liver.

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