Insulin-like growth factor binding protein-3 in normal pubertal girls

1992 ◽  
Vol 126 (5) ◽  
pp. 381-386 ◽  
Author(s):  
Darrell M Wilson ◽  
Mark A Stene ◽  
Joel D Killen ◽  
Lawrence D Hammer ◽  
Iris F Litt ◽  
...  
Keyword(s):  
Pubertal Development ◽  
Red Cell ◽  
Pubertal Stage ◽  
Health Curriculum ◽  
School Based ◽  
Igf I ◽  
Pubertal Stages ◽  
Nutrition Intake ◽  
The Mean ◽  
Igfbp 3

IGFBP-3 concentrations rise in the second decade of life. To test the hypothesis that the stage of pubertal development, independent of chronological age, was associated with these increases we measured serum IGFBP-3 concentrations by radioimmunoassay in 324 sixth and seventh grade girls (12.3±0.7 years) at the beginning of a multisite school-based health curriculum. The mean (±sd) serum IGFBP-3 among the 242 girls with complete data was 4.0±0.7mg/l. Pubertal stage was significantly associated with IGFBP-3 (p<0.0001, ANOVA). Mean concentrations rose from 3.5±0.7 mg/l among those with the earliest pubertal stages to 4.2±0.7mg/l among the mature girls. IGF-I and IGFBP-3 concentrations were significantly correlated (Spearman's r=0.43, p<0.0001). After controlling for the association between pubertal development and IGFBP-3 concentrations, only the waist/hip ratio, among the various measures of body composition, was significantly associated with IGFBP-3 concentration (Spearman's r= −0.23, p=0.0002). Likewise, none of the measures of nutrition: intake of total calories, protein, fat and carbohydrate; serum iron; red cell mean corpuscular volume; or cholesterol; were significantly associated with IGFBP-3 concentrations. There was, however, a small, but significant association between IGFBP-3 concentrations and both serum transferrin and blood hemoglobin concentrations. Pubertal stage has a significant impact on IGFBP-3 concentrations and those attempting to utilize IGFBP-3 concentrations during adolescence should be cognizant of the subject's pubertal stage.

Relationships Between Levels of Leptin and Hematological Parameters in Healthy Term Infants

2001 ◽  
Vol 14 (8) ◽  
Author(s):  
Esin Koç ◽  
Aysun Bideci ◽  
Peyami Cinaz ◽  
Ebru Ergenekon ◽  
Yıldız Atalay
Keyword(s):  
Hematological Parameters ◽  
Serum Concentrations ◽  
Term Infants ◽  
Positive Correlation ◽  
Blood Hemoglobin ◽  
Igf I ◽  
The Mean ◽  
Igfbp 3

AbstractIn order to evaluate the role of leptin in neonatal hematological parameters, we studied the serum concentrations of leptin in relation to blood hemoglobin, leukocyte and platelet values in 30 healthy term infants. We also studied the serum concentrations of IGF-I and IGFBP-3 in relation to leptin concentrations. The mean concentrations of leptin, IGF-I and IGFBP-3 were 1.63 ± 1.09, 24.65 ± 10.04 and 976.05 ± 214.50, respectively, at birth. A positive correlation was observed between leptin concentrations and birth weights of the infants. As no relationship could be found between concentrations of leptin and blood hemoglobin, leukocyte and platelet values, we could not determine any involvement of leptin in the regulation of physiologial hemoglobin, leukocyte and platelet concentrations at birth.

Radioimmunoassay of insulin-like growth factor-binding protein-6 in human serum and other body fluids

1992 ◽  
Vol 134 (1) ◽  
pp. 133-139 ◽  
Author(s):  
R. C. Baxter ◽  
H. Saunders
Keyword(s):  
Cerebrospinal Fluid ◽  
Growth Factor ◽  
Human Serum ◽  
Follicular Fluid ◽  
Binding Protein ◽  
Insulin Like Growth Factor ◽  
Factor Binding ◽  
Igf I ◽  
The Mean ◽  
Igfbp 3

ABSTRACT A radioimmunoassay has been established for the insulin-like growth factor-binding protein, IGFBP-6, isolated from a human transformed fibroblast cell-line. The binding proteins IGFBP-I and IGFBP-3 did not cross-react, but both IGF-I and IGF-II markedly inhibited IGFBP-6 tracer binding to antiserum. This inhibition, greater for IGF-II than for IGF-I, was fully reversed by the addition of IGFBP-3 to sequester the IGFs. After fractionation of human serum and follicular fluid samples by gel chromatography, interference in the radioimmunoassay by fractions corresponding to the 150 kDa IGF-IGFBP complex could be eliminated by IGFBP-3. The equivalent fractions from cerebrospinal fluid and amniotic fluid fractionation did not interfere in the assay. The mean IGFBP-6 level in adult human serum was 0·221 ±0·110 mg/l, with values significantly higher in men than women, and slightly decreased in pregnancy. Similar values were seen in umbilical cord serum and in amniotic and follicular fluid samples, while the mean level in cerebrospinal fluid was slightly lower, 0·152±0·049 mg/l. This assay will facilitate studies on the regulation of IGFBP-6 production, and its role as an IGF carrier. Journal of Endocrinology (1992) 134, 133–139

scholarly journals Twenty-Four-Hour Profiles of Luteinizing Hormone, Follicle-Stimulating Hormone, Testosterone, and Estradiol Levels: A Semilongitudinal Study throughout Puberty in Healthy Boys*

1997 ◽  
Vol 82 (2) ◽  
pp. 541-549 ◽  
Author(s):  
Kerstin Albertsson-Wikland ◽  
Sten Rosberg ◽  
Birgitta Lannering ◽  
Leo Dunkel ◽  
Gunnar Selstam ◽  
...  
Keyword(s):  
Diurnal Rhythm ◽  
Pubertal Development ◽  
Tanner Stage ◽  
Testicular Volume ◽  
Early Puberty ◽  
Pubertal Stage ◽  
Pubertal Stages ◽  
Prepubertal Boys ◽  
Stage 1 ◽  
Estradiol Levels

Abstract To follow and correlate gonadotropin and sex steroid changes throughout puberty, 24-h profiles of LH, FSH, testosterone, and estradiol were taken on several occasions for between 2–9.5 yr in 12 healthy boys, aged 8.7–18.2 yr. Serum concentrations of LH and FSH were measured every 20 min, whereas testosterone and estradiol were measured every 2–4 h during the 24-h period. The prepubertal boys (Tanner stage 1) were subdivided into two groups: Pre 1, with a testicular volume of 1–2 mL, and Pre 2, with a testicular volume of 3 mL. Pubertal stages were classified, according to testicular volume, as early puberty (pubertal stage 2; 4–9 mL), midpuberty (pubertal stages 3–4; 10–15 mL), and late puberty (pubertal stage 5; ≥16 mL). Mean levels of LH and FSH increased with pubertal development, although the increase in LH was greater than that in FSH. These increases were due to elevated basal levels of LH and FSH as well as to increases in the number of peaks and the peak amplitudes of LH. No diurnal rhythm was found in boys at stage Pre 1. Thereafter, a clear diurnal rhythm appeared for LH, and later in puberty, an ultradian rhythm was superimposed, as shown by time-sequence analyses. A diurnal rhythm also existed for FSH, but was much less marked than that for LH despite a clear covariation between LH and FSH, as shown from cross-correlation studies. Testosterone also showed diurnal variations from the late prepubertal stage, followed by increasing levels during both day and night in puberty. We conclude that during puberty, gonadotropin levels rise differently for LH and FSH, which may be due to the development of differences in feedback mechanisms. Despite covariation between LH and FSH, only LH showed a clear diurnal variation. In parallel, nocturnal variations in testosterone and estradiol were found. Changes in mean levels of LH, testosterone, and estradiol as well as their mean daytime and nighttime levels follow each other from the prepubertal stages to late puberty.

Growth hormone secretion in pubertal and adult subjects

1982 ◽  
Vol 99 (2) ◽  
pp. 161-165 ◽  
Author(s):  
Francesco Minuto ◽  
Antonina Barreca ◽  
Silvano Ferrini ◽  
Giancarlo Mazzocchi ◽  
Patrizia Del Monte ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Hormone Secretion ◽  
Growth Hormone Secretion ◽  
Pubertal Stage ◽  
Pubertal Maturation ◽  
Significant Difference ◽  
Pubertal Stages ◽  
The Mean ◽  
Stage 1 ◽  
Mean Concentration

Abstract. GH responsiveness to insulin-induced hypoglycaemia and its circadian secretion were studied in a group of subjects in different pubertal stages. The GH peak after insulin was minimal in stage 1 boys (7.6 ± 1.3 (sem) ng/ml) and increased progressively, in parallel with pubertal maturation, reaching a maximum in the adult state (20 ± 4.0 ng/ml); the basal value was superimposable in all groups studied. The circadian secretion showed a sleep-related surge which was almost identical in the different stages; however in pubertal stage 1 boys multiple secretory peaks were observed, mainly during waking hours. A statistically significant difference (P < 0.05) was observed in the mean concentration recorded during the day with a maximum in stage 1 boys (2.7 ± 0.2 ng/ml) and a gradual decrease to a minimum in adults (1.2 ± 0.3 ng/ml). This pattern seems to suggest that a pulsatile rhythm is present in boys, similar to that observed for gonadotrophins.

Anthropometric, biochemical and hormonal profiles of the partially admixed pygmoid group in Rampasasa (Flores, Indonesia)

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Aman Pulungan ◽  
Attika A. Andarie ◽  
Frida Soesanti ◽  
Muhammad Ramdhani Yassien ◽  
Christiaan de Bruin ◽  
...  
Keyword(s):  
Head Circumference ◽  
Standard Deviation Score ◽  
Bone Age ◽  
Skeletal Maturation ◽  
Delayed Puberty ◽  
Pubertal Stage ◽  
Sitting Height ◽  
Arm Span ◽  
Igf I ◽  
Igfbp 3

Abstract Objectives We performed a cross-sectional study on anthropometric and laboratory characteristics of inhabitants of Rampasasa (Flores, Indonesia). Adults were categorised according to ancestry into three groups: pygmoid (P/P, offspring of pygmoid parents, n=8), mixed pygmoid (P/N, offspring of pygmoid and non-pygmoid parents, n=12) and non-pygmoid (N/N, n=10). Children (n=28) were P/N. Methods Measurements included height, weight, sitting height, arm span, head circumference, haematological analysis and serum albumin, calcium, vitamin D, insulin-like growth factor-I (IGF-I) and IGF binding protein 3 (IGFBP-3). Pubertal stage and bone age was assessed in children. Anthropometric data were expressed as standard deviation score (SDS) for age. IGF-I, IGFBP-3 and IGF-I/IGFBP-3 ratio were expressed for age, bone age and pubertal stage. Results Mean height SDS showed a gradient from P/P (−4.0) via P/N (−3.2) to N/N (−2.3) (−3.4, −3.1 and −2.2 adjusted for age-associated shrinking). Sitting height and head circumference showed similar gradients. Serum IGF-I SDS was similar among groups (approximately −1 SDS). IGFBP-3 SDS tended toward a gradient from P/P (−1.9) via P/N (−1.5) to N/N (−1.1), but IGF-I/IGFBP-3 ratio was normal in all groups. In P/P and P/N, mean head circumference SDS was >2 SD greater than mean height SDS. Children showed a progressive growth failure and bone age delay, delayed female pubertal onset and an initial low serum IGF-I, normal IGFBP-3 and low IGF-I/IGFBP-3 ratio. Conclusions P/P showed proportionate short stature with relative macrocephaly and relatively low IGFBP-3; P/N presented an intermediate pattern. P/N children were progressively short, showed delayed skeletal maturation, delayed puberty in girls and low IGF-I and IGF-I/IGFBP-3.

scholarly journals The relationship between the GH/IGF-I axis and serum markers of bone turnover metabolism in healthy children

2007 ◽  
Vol 157 (5) ◽  
pp. 685-692 ◽  
Author(s):  
Juliane Léger ◽  
Isabelle Mercat ◽  
Corinne Alberti ◽  
Didier Chevenne ◽  
Priscilla Armoogum ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Turnover ◽  
Bone Metabolism ◽  
Healthy Children ◽  
Weak Correlation ◽  
Pubertal Stage ◽  
Cross Sectional ◽  
Bone Metabolism Markers ◽  
Igf I ◽  
Igfbp 3

AbstractContextThere is evidence to suggest that IGF-I plays a role in regulating bone turnover.ObjectiveTo evaluate the relationships between serum concentrations of IGF-I and IGF-binding protein-3 (IGFBP-3), and bone metabolism markers in healthy children.Design and settingProspective cross-sectional study.Subjects and methodsA cohort of 579 boys and 540 girls, all healthy Caucasian, were included in this study. Serum IGF-I and IGFBP-3 concentrations, bone alkaline phosphatase (BAP) and CrossLaps (markers of bone formation and bone resorption respectively) levels were evaluated as a function of age, gender, pubertal stage and body mass index.ResultsSerum IGF-I SDS levels were positively correlated with BAP and CrossLaps SDS levels before and after puberty, and also with CrossLaps during puberty (weak correlation). Serum IGFBP-3 SDS levels were positively correlated with BAP and CrossLaps levels before, during (weak correlation) and after puberty (for BAP levels only).ConclusionsThis study demonstrated the independent association between serum IGF-I and IGFBP-3 concentrations with both serum bone formation and resorption markers in healthy children. Physiological differences before, during and after puberty in the association of serum IGF-I and IGFBP-3 levels with the serum bone metabolism markers were found. These differences may be related to differences in interactions between sex steroid hormones and the GH/IGF-I system, bone metabolism and growth during the pubertal transition. Improvements in our understanding of life course determinants of the IGF-I system and bone metabolism are required to shed further light on the role of the GH/IGF-I axis in bone remodelling.

scholarly journals Estrogen Priming Effect on Growth Hormone (GH) Provocative Test: A Useful Tool for the Diagnosis of GH Deficiency1

2000 ◽  
Vol 85 (11) ◽  
pp. 4168-4172 ◽  
Author(s):  
Alicia S. MartÍnez ◽  
Horacio M. Domené ◽  
M. Gabriela Ropelato ◽  
Héctor G. Jasper ◽  
Patricia A. Pennisi ◽  
...  
Keyword(s):  
Maximal Response ◽  
Diagnostic Efficiency ◽  
Provocative Test ◽  
Igf I ◽  
Stimulation Tests ◽  
Igf Binding ◽  
The Mean ◽  
Igf Binding Protein ◽  
Confidence Interval Limit ◽  
Igfbp 3

We have studied the effect of estradiol (E2)on the GH-insulin-like growth factor (GH-IGF) axis in 15 prepubertal GH deficiency (GHD) children and 44 prepubertal or early pubertal children with idiopathic short stature (SS). All of them received a daily dose of micronized E2 (1 or 2 mg) or placebo, for 3 days, before a sequential arginine-clonidine test. In SS children, GH maximal responses were 17.8 ± 10.9 on placebo and 27.9 ± 14.5μ g/L on estrogen (P &lt; 0.0001). The lower 95% confidence limits for GH maximal response changed from 3.7 μg/L (without E2) to 8.3 μg/L (on E2). In GHD children, no significant stimulatory effect of estrogen on GH levels was observed. After placebo, a cut-off limit of 3.7 μg/L (the lower 95% confidence interval limit) resulted in 73% sensitivity, 95% specificity, and an overall 90% diagnostic efficiency. After E2, a cut-off limit of 8.3 μg/L resulted in a sensitivity of 87%, a specificity of 98%, and a diagnostic efficiency of 95%. After placebo, 68% of SS showed normal IGF-I levels, and the mean did not change on E2 (13.7 ± 6.3 vs. 14.3 ± 6.8 nmol/L, not significant). In 93% of SS, IGF binding protein (IGFBP)-3 levels were normal during placebo. On E2, mean IGFBP-3 did not change (2.63 ± 0.70 vs. 2.70 ± 0.70 mg/L, not significant). In 14 of 15 GHD patients, IGF-I values were below normal on placebo, and the mean of the group did not change after E2. During placebo, 13 of 15 GHD children presented low IGFBP-3 values. During E2, there was a small significant increase in IGFBP-3 values (1.06 ± 0.58 vs. 1.20 ± 0.69 mg/L, P &lt; 0.02). The highest diagnostic efficiencies for IGF-I and IGFBP-3 were observed during placebo (75% and 91%, respectively). We conclude that GH stimulation tests after E2 priming had the highest diagnostic efficiency. Our findings suggest that the effect of estrogen priming on GH stimulated levels, by reducing the number of false nonresponders, might be useful to better discriminate between normal and abnormal GH status in SS children.

scholarly journals Sexual dimorphism in cortisol metabolism throughout pubertal development: a longitudinal study

2020 ◽  
Vol 9 (6) ◽  
pp. 542-551
Author(s):  
Britt J van Keulen ◽  
Conor V Dolan ◽  
Bibian van der Voorn ◽  
Ruth Andrew ◽  
Brian R Walker ◽  
...  
Keyword(s):  
Sexual Dimorphism ◽  
Excretion Rate ◽  
Reductase Activity ◽  
Pubertal Development ◽  
Tanner Stage ◽  
Early Morning ◽  
Considerable Decrease ◽  
Pubertal Stage ◽  
Cortisol Metabolism ◽  
Pubertal Stages

Objective Sex differences in disease susceptibility might be explained by sexual dimorphism in hypothalamic-pituitary-adrenal axis activity, which has been postulated to emerge during puberty. However, studies conducted thus far lacked an assessment of Tanner pubertal stage. This study aimed to assess the contribution of pubertal development to sexual dimorphism in cortisol production and metabolism. Methods Participants (n = 218) were enrolled from a population-based Netherlands Twin Register. At the ages of 9, 12 and 17 years, Tanner pubertal stage was assessed and early morning urine samples were collected. Cortisol metabolites were measured with GC-MS/MS and ratios were calculated, representing cortisol metabolism enzyme activities, such as A-ring reductases, 11β-HSDs and CYP3A4. Cortisol production and metabolism parameters were compared between sexes for pre-pubertal (Tanner stage 1), early pubertal (Tanner stage 2–3) and late-pubertal (Tanner stage 4–5) stages. Results Cortisol metabolite excretion rate decreased with pubertal maturation in both sexes, but did not significantly differ between sexes at any pubertal stage, although in girls a considerable decrease was observed between early and late-pubertal stage (P < 0.001). A-ring reductase activity was similar between sexes at pre- and early pubertal stages and was lower in girls than in boys at late-pubertal stage. Activities of 11β-HSDs were similar between sexes at pre-pubertal stage and favored cortisone in girls at early and late-pubertal stages. Cytochrome P450 3A4 activity did not differ between sexes. Conclusions Prepubertally, sexes were similar in cortisol parameters. During puberty, as compared to boys, in girls the activities of A-ring reductases declined and the balance between 11β-HSDs progressively favored cortisone. In addition, girls showed a considerable decrease in cortisol metabolite excretion rate between early and late-pubertal stages. Our findings suggest that the sexual dimorphism in cortisol may either be explained by rising concentrations of sex steroids or by puberty-induced changes in body composition.

Are plasma Insulin-Like Growth Factor I (IGF-I) and Igf-Binding Protein 3 (Igfbp-3) Useful Markers of Prostate Cancer?

2004 ◽  
Vol 19 (2) ◽  
pp. 164-167 ◽  
Author(s):  
J.B. Lopez ◽  
R.M. Sahabudin ◽  
L.P. Chin
Keyword(s):  
Prostate Cancer ◽  
Growth Factor ◽  
Binding Protein ◽  
Specific Antigen ◽  
Insulin Like Growth Factor ◽  
Factor I ◽  
Igf I ◽  
The Mean ◽  
Growth Factor I ◽  
Igfbp 3

Increased concentrations of insulin-like growth factor I (IGF-I) and decreased insulin-like growth factor binding protein 3 (IGFBP-3) in serum have been proposed as markers of prostate cancer (CaP). The evidence for this, however, is contradictory. We assayed serum for IGF-I, IGFBP-3 and prostate-specific antigen (PSA) in patients with CaP and benign prostatic hyperplasia (BPH) and in healthy controls (HC). The mean ± SD concentration of IGF-I in CaP (98.3 ± 39.3 ng/mL; n=15) was lower than in BPH (119 ± 31.1 ng/mL; n=24) and HC (119 ± 36.1 ng/mL; n=46), but the differences between the three groups were not statistically significant (p>0.05). The mean IGFBP-3 concentrations in CaP (2691 ± 1105 ng/mL; n=16; p=0.029) and BPH (2618 ± 816 ng/mL; n= 26; p=0.006) patients were significantly lower than that of the HC (3119 ± 618 ng/mL; n=59), but the difference between the two groups of patients was not significant (p>0.05). PSA concentrations in CaP (median=80.8 ng/mL; n=25) were significantly higher than those in BPH (median=8.6 ng/mL; n=39) (p<0.001). Ninety-six percent of CaP and 72% of BPH patients had PSA concentrations >4.0 ng/mL; the proportions of patients with concentrations exceeding 20 ng/mL were 76% and 10%, respectively. We conclude that IGF-I and IGFBP-3 are inferior to PSA for CaP detection.

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