The effect of glibenclamide on plasma insulin, plasma somatomedin bioactivity and skeletal growth in hypophysectomized rats

1982 ◽  
Vol 101 (2) ◽  
pp. 187-192 ◽  
Author(s):  
E. Heinze ◽  
M. Ranke ◽  
E. Manske ◽  
U. Vetter ◽  
K.-H. Voigt
Keyword(s):  
Body Weight ◽  
Serum Insulin ◽  
The Body ◽  
Skeletal Growth ◽  
Male Rats ◽  
Epiphyseal Growth Plate ◽  
Body Weights ◽  
Hypophysectomized Rats ◽  
Group A ◽  
Group B

Abstract. Male rats, body weight 60–75 g, were hypophysectomized. Three days after operation the animals were divided into two groups. Group B received solvent solution and group C 1 mg/kg body weight per day of glibenclamide ip for the following 9 days. Group A consisted on non-operated normal rats. Twenty-four hours after the last injections and after a 12 h overnight fast the body weights of groups B and C were not different, the increase during the 10 days being 10% in both groups. Serum insulin (IRI) was significantly higher in group C than in group B (C: 8.0 ± 0.3 μU/ml, n = 14 vs B: 4.9 ± 1.0 μU/ml, n = 14; P < 0.01, mean ± sem) as was serum somatomedin bioactivity (SM)-porcine cartilage assay — (C: 1.06 ± 0.1 U/ml, n = 14 vs B:0.41 ± 0.01 U/ml, n = 14; P < 0.001). Skeletal growth was determined with the tibia test and by a radiograph of each rat. The width of the proximal epiphyseal growth plate of the tibia was significantly increased in group C compared to group B (C: 204 ± 4.8 μm, n = 12 vs 181 ± 6.5 μm, n = 13; P < 0.005). On the radiograph the area of the right femur was not different between the two groups of animals, while the height and the area of the first lumbar spine were significantly augmented in group C. The results show that glibenclamide stimulates IRI, SM and skeletal growth in hypophysectomized rats. Compared to the glibenclamide treated hypophysectomized animals the normal rats of group A had doubled their body weights. IRI (59 ± 5 μU/ml, n = 4) and skeletal growth (tibia test: 454 ± 5.8 μm) were greatly increased. SM did not differ between group A (1.21 ± 0.35 U/ml and group C. T4 was much lower in group B (0.64 ± 0.09 μg/100 ml, n = 5) than in group A (4.1 ± 0.3 μg/100 ml, n = 6; P < 0.001). It is concluded that a normal SM concentration is not necessarily associated with appropriate growth.

scholarly journals MORPHOMETRICAL ANALYSIS OF MAJOR LYMPHOID ORGANS OF CHEMOTHERAPY TREATED CHICKENS

2012 ◽  
Vol 3 (2) ◽  
pp. 106-109 ◽  
Author(s):  
MR Karim ◽  
MZI Khan ◽  
Z Haque
Keyword(s):  
Body Weight ◽  
Lymphoid Organs ◽  
The Body ◽  
Bursa Of Fabricius ◽  
Morphometrical Analysis ◽  
Body Weights ◽  
Group A ◽  
Cervical Subluxation ◽  
Group B ◽  
Group D

The study was carried out to know the gross morphological and morphometrical changes of major lymphoid organs of chemotherapy treated chickens during the period from July to September 2004 in the Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh. The 12-day-old 16 chickens (Vencobb broiler) were divided into four groups viz, group A, group B, and group C that were treated with cyclophosphamide @ 3 mg / kg body weight intramuscularly, vincristine @ 0.07 mg /kg body weight intramuscularly and the combination of the former two drugs using same dose and route for 3 consecutive days respectively and group D was kept as untreated control. After 7 days of last chemotherapy all birds were weighed and killed one by one through cervical subluxation. The body weights of the chemotherapy treated groups were decreased in comparison to control but more decreased in cyclophosphamide treated chickens. The major lymphoid organs (thymus, bursa of Fabricius, spleen and cecal tonsils) were exposed through ventral neck and abdominal dissection and grossly atrophy of the major lymphoid organs was observed in the chemotherapy treated chicken than the control chicken. After collection, the major lymphoid organs were weighed with the help of electric balance one by one. The weights of major lymphoid organs reduced in the chemotherapy treated chickens than the control chickens but the weight of lymphoid organs more reduced in the cyclophosphamide treated chickens. The result of the present study revealed that the size and weight of the major lymphoid organs of the chemotherapy treated chicken decreased in comparison to control chicken. These results indicated that the reduction of weight of major lymphoid organs was due to the action of drug in the lymphoid organs of chemotherapy treated chickens.

scholarly journals Effect of certain haematinics on body weight and haemato-biochemical changes in laboratory mice

1970 ◽  
pp. 103-105
Author(s):  
M Jahan ◽  
N Ahmad ◽  
M Myenuddin
Keyword(s):  
Body Weight ◽  
Vitamin B12 ◽  
Biochemical Changes ◽  
The Body ◽  
Treatment Groups ◽  
Group A ◽  
The Difference ◽  
Group B ◽  
Physiology Laboratory ◽  
Hb Concentration

A total of 24, six weeks old mice were used to study the body weight and haemato-biochemical changes following administering of different haematinics (CuSO4, FeSO4 and Vitamin B12). The experiment was performed in Physiology laboratory, Department of Physiology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, from February to March 2006. They were randomly assigned to one of four equal groups (n = 6). In addition to normal rat pellets Group A was supplemented with CuSO4 @ 0.6mg/mice/day orally, Group B with FeSO4 @ 1.2mg/mice/day orally, and Group C with Vitamin B12 (Cytamin®, Glaxo) @ 0.004mg/mice intramuscularly at every 7 days. Group D was considered as control and was also supplemented with rat pellets. Increased body weight was observed in all haematinic treated mice but group B had a significant (p < 0.05) higher weight gain compared to the control. TEC and PCV increased significantly (p < 0.05) in all the treatment groups compared to the control. Statistically insignificant (p > 0.05) increase in TLC was also recorded in all the treated groups. Hb concentration increased for all the treatment groups. The ESR values of the experimental mice were negligible in first hour. The serum transaminases (SGPT and SGOT) increased in all treated groups but the difference in group A was significant (p < 0.05) compared to the control. Blood urea was recorded significantly (P < 0.01) higher for group A and lower for group C compared to the control. This study can be helpful to study the effect of particular haematinic on animal especially on simple stomach animal and human being as mice is used as human and animal model. Key words: Haematinics, haemato-biochemical parameters, mice DOI = 10.3329/bjvm.v5i1.1325 Bangl. J. Vet. Med. (2007). 5 (1 & 2): 103-105

scholarly journals Effects of edible fats and oils on the body weight gain and on weights of some selected organs in rats removing the impact of unequal feed intake

1970 ◽  
pp. 107-110
Author(s):  
N Ahmad ◽  
S Majumder ◽  
MA Miah ◽  
MJ Uddin
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Feed Intake ◽  
Body Weight Gain ◽  
Fats And Oils ◽  
Male Rats ◽  
Edible Fats ◽  
Group A ◽  
Group B ◽  
The Impact

An investigation on Long Evans male rats fed with different edible fats and oils was conducted in the Department of Physiology, Bangladesh Agricultural University, Mymensingh during a period of 7 weeks (1st April to 19th May, 2005) to determine and to compare the effect of feeds on body weight gain and on weights of some selected organs (heart, liver and kidney) removing the impact of unequal feed intake. A total of 20, six-week old male rats were randomly divided into A, B, C and D groups. Each group consisted of 5 rats. Rats were fed rat pellets purchased from ICDDR,B, Dhaka supplemented with beef fat in group A, fish fat in group B and soybean oil in group C while group D was considered as control and fed only with rat pellets. The concentration of fats and oils were 7% of normal diet and fed for 7 weeks. The highest weekly mean body weight gain (19.90g) adjusted for unequal feed intake was achieved by the rats of beef fat supplemented group A, followed by the rats of soybean oil supplemented group C (19.76g) and fish fat supplemented group B (15.67g). But none of the adjusted means of weekly body weight gain differed significantly (p > 0.05) from the control. Insignificant increases in heart weight were recorded in all treated rats and the maximum weight was in fish oil treated ones. Not much differences were recorded in the kidney weights rather beef oil treated rats' kidney had the lowest mean weight. A significantly (p < 0.01) higher liver weight was recorded in group B & C compared to control (group D), though the differences between A & D were insignificant. It could be concluded that fats and oils are harmful for the rat's body especially on liver and heart. Key words: Edible fats and oils, rat, body weight, organ weight, analysis of variance, covariance DOI = 10.3329/bjvm.v5i1.1326 Bangl. J. Vet. Med. (2007). 5 (1 & 2): 107-110

ABSENCE OF GROWTH IN HYPOPHYSECTOMIZED RATS TREATED WITH THYROID HORMONES

1966 ◽  
Vol 51 (2) ◽  
pp. 315-320 ◽  
Author(s):  
C. M. Goodall ◽  
J. B. Gavin
Keyword(s):  
Drinking Water ◽  
Body Weight ◽  
Thyroid Hormones ◽  
Body Mass ◽  
The Body ◽  
Male Rats ◽  
Radiographic Measurements ◽  
Morphological Criteria ◽  
Hypophysectomized Rats ◽  
Stimulation Of

ABSTRACT Male rats were hypophysectomized at four weeks of age, rested for four weeks, and then were fed a thyroid digest in their drinking water continuously for up to seventy weeks. The digest treatment supplied the equivalent of 1.25μg L-thyroxine per 100 g rat per day approximately. From the body weight curves and radiographic measurements of the skull and mandibles, it was shown that contrary to some previous reports in the literature there was no statistically significant change in the indices of growth or body mass when compared with controls receiving no thyroid treatment. It is suggested that data previously reported by others, indicating a stimulation of growth in hypophysectomized rats by thyroid hormones, may have been derived from animals having minute but still effective pituitary remnants, and somewhat more stringent morphological criteria of hypophysectomy are proposed.

scholarly journals Modified Dietetic Skim And Cotton Sheet Oil Dan Susu Formula 75 Terhadap Berat Badan Anak Balita Gizi Kurang

2021 ◽  
Vol 16 (3) ◽  
pp. 656-661
Author(s):  
Pembronia Nona Fembi ◽  
Yosefina Nelista
Keyword(s):  
Body Weight ◽  
T Test ◽  
The Body ◽  
Nutritional Deficiencies ◽  
P Value ◽  
Malnourished Children ◽  
Group A ◽  
Group B ◽  
The Impact ◽  
Paired T Test

  Children under five are a group that is prone to malnutrition because they are still experiencing a growth cycle, a development that requires more nutrients than other age groups. The impact of nutritional deficiencies; low body resistance, the body is susceptible to infectious diseases. The purpose of this study was to analyze the effectiveness of giving modified dietetic skim and cotton sheet oil (MODISCO) and Formula-75 milk to increase body weight of malnourished children in Puskesmas Waigete, Sikka Regency. The type of research used is Quasy experimental pretest and posttest design. The population of this research was 181 underweight children with malnutrition. The sampling technique used purposive sampling. The sample of this study was 24 underweight children with malnutrition, divided into 2 (two), namely group A was given MODISCO intervention and group B was given Formula-75 milk. The intervention was given once a day for 1 month at a dose of 150 kcal / kgbb / day for MODISCO and 1000 ml per day for 75 formula milk. Data analysis used Paired t-Test and Independent Samples Test. Paired T-Test analysis, groups A and B obtained P-value (0.000) <0.05, it was concluded that there was an increase in body weight after being given MODISCO and Formula-75 milk. Based on the analysis of the Independent Samples Test, groups A and B obtained a P-value (0.876)> 0.05, it was concluded that there was no difference in body weight for under-nourished toddlers who were given MODISCO and Formula-75 milk. There is no difference in body weight of malnourished underweight children in group A and group B, so it is concluded that MODISCO and Formula-75 milk can increase the weight of malnourished children underweight. The results of this study are an additional intervention to increase the weight of children under malnutrition.

EFFECT OF POLYDIETHYLSTILBOESTROL PHOSPHATE ON THE PITUITARY CONTENT OF FSH AND ICSH IN MALE RATS

1969 ◽  
Vol 61 (3) ◽  
pp. 449-460 ◽  
Author(s):  
Torsten Perklev ◽  
Yngve Gröning
Keyword(s):  
Body Weight ◽  
Interstitial Cell ◽  
The Body ◽  
Male Rats ◽  
Inhibiting Effect ◽  
Accessory Glands ◽  
Complete Restoration ◽  
Body Weights ◽  
Human Menopausal Gonadotrophin ◽  
Stimulating Hormone

ABSTRACT The effect of a single dose of polydiethylstilboestrol phosphate (PSP) on the pituitary content of follicle stimulating hormone (FSH) and interstitial cell stimulating hormone (ICSH) was studied in intact, adult male rats after 7, 28, 103 and 161 days, respectively. The body weight and different organ weights were also recorded. The results were compared with those obtained in untreated controls and in rats treated with daily doses of diethylstilboestrol (DES) for 7 days. The pituitary FSH and ICSH content in the control rats decreased roughly to about half during a period of approximately 5 months, i. e. from the 2nd to the 7th month of life. PSP (400 μg/100 g body weight) and DES (320 μg/100 g body weight) reduced the pituitary content of FSH and ICSH to approximately 35 and 50% of the control values, respectively, measured on the 7th day of treatment. The gonadotrophin inhibiting effect of PSP lasted for more than 103 days. On the other hand, on Day 28 the pituitary content of ICSH was normal and that of FSH significantly elevated in the DES-treated rats. On Day 161 both PSP- and DES-treated animals had a significantly increased ICSH content, while the FSH content was normal. Body weights, as well as the weights of the testes and accessory glands were reduced both by DES and PSP as early as on the 7th day after the commencement of treatment. Complete restoration of these parameters in DES-treated animals was found on Day 103, while PSP exerted its effect for more than 161 days. Daily injections of 3 IU of FSH and 1.5 IU of ICSH administered in the form of human menopausal gonadotrophin (HMG) to PSP-treated rats from the day of PSP injection for 14 days counteracted the weight loss of the testes and accessory glands, as well as the loss of body weight and the reduced food intake. The hypertrophy of the adrenals and the sterility produced by the administration of PSP were completely prevented by HMG injections.

scholarly journals Effects of growth promoter and multivitamin-mineral premix supplementation on body weight gain in broiler chickens

2013 ◽  
Vol 10 (2) ◽  
pp. 245-248 ◽  
Author(s):  
MA Rahman ◽  
MS Parvin ◽  
RR Sarker ◽  
MT Islam
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Broiler Chickens ◽  
Body Weight Gain ◽  
The Body ◽  
Growth Promoter ◽  
Commercial Feed ◽  
Significant Difference ◽  
Group A ◽  
Group B

An experiment was conducted at the Khanpura Poultry Farm, near to the Babugonj campus of Patuakhali Science and Technology University, Patuakhali during the period from August to September, 2011 to evaluate the effect of growth promoter and multivitamin-mineral premix on the body weight gain in broiler chickens. A total of 90 ‘Cobb 500’ day-old broiler chickens were randomly divided into three equal groups (n = 30), namely Groups A, B and C. Broiler birds in groups A and B were supplemented with growth promoter and multivitamin-mineral premix, respectively in addition to commercial feed from day 4 till day 25. The dose of both the growth promoter and multivitamin-mineral premix was 2.5 g/kg of feed. Chickens of group C served as control and supplemented with commercial feed only. Body weight was recorded at three times as on day 11, 18 and 25, respectively. The mean body weight was insignificantly (p > 0.05) higher in birds of groups A (992.5±139.6g) and B (978.3±147.0g) than those of group C (926.7±133.2g). The percent increase in body weight gain compare to control was increased in both the supplemented groups (5.8% in group A and 8.8% in group B) though there was no significant difference. Based on the results, it is suggested that supplementation of growth promoter and multivitamin-mineral premix in broilers even after feeding of commercial feed has positive effect on the growth of broilers.DOI: http://dx.doi.org/10.3329/jbau.v10i2.14914 J. Bangladesh Agril. Univ. 10(2): 245-248, 2012

scholarly journals Comparative study of the performance of buffalo calves and cow calves by feeding Urea Molasses Block with straw based diet

1970 ◽  
Vol 8 (1) ◽  
pp. 87-90
Author(s):  
S Ferdousi ◽  
AKM Masum ◽  
MAS Khan ◽  
MA Islam
Keyword(s):  
Body Weight ◽  
Body Length ◽  
Body Weight Gain ◽  
The Body ◽  
Buffalo Calves ◽  
Final Body Weight ◽  
Height Gain ◽  
Group A ◽  
Group B ◽  
Average Body

An experiment was conducted to evaluate the performance of buffalo calves and cow calves supplementing Urea Molasses Block (UMB) with straw based diet. Three cow calves (average 1 year & 8 months age and 111 kg body weight) and three buffalo calves (average 1 year & 10 months age and 89 kg body weight) were grouped into A and B, respectively and fed for 60 days. It was found that, the DMI (kg/h/d) was 5.66±1.18 and 6.10±0.99, respectively in group A and B (p>0.05). The DMI in 1st and 2nd fortnight of the experiment in group A and B were 5.77±0.31 and 6.17±0.33; 4.96±0.43 and 5.77±0.39, respectively (p<0.01). But, the DMI in 3rd and 4th fortnight differs nonsignificantly (p>0.05). At the end of the experiment, the final body weight for group A and group B were 132.6±11.96 and 113±8.19 kg, respectively (p>0.05). The body weight gain per day for both groups were 0.36±0.05 and 0.40±0.05 kg, respectively (p>0.05). The average heart girth gain per day in group A and group B were 0.24±0.02 and 0.30±0.03 cm, respectively (p>0.05). The average wither height gain per day was 0.075±0.005 and 0.059±0.005 cm in group A and B, respectively (p<0.05). The average body length gain per day for group A and group B were 0.31±0.03 and 0.36±0.04 cm, respectively (p>0.05). The growth performance of buffalo calves is better than cow calves by feeding UMB with straw based diet. Keywords: UMB; Body weight; Heart girth; Wither height; Body length DOI: 10.3329/jbau.v8i1.6404J. Bangladesh Agril. Univ. 8(1): 87-90, 2010

scholarly journals CELECOXIB

2018 ◽  
Vol 25 (01) ◽  
pp. 50-57
Author(s):  
Sadia Sundus ◽  
Nazia Qamar ◽  
Raheela Adil ◽  
Muhammad Faisal Fahim
Keyword(s):  
Body Weight ◽  
Relative Weight ◽  
Treated Group ◽  
Renal Tissue ◽  
The Body ◽  
Control Group ◽  
Albino Rats ◽  
Group A ◽  
Group B ◽  
General Architecture

Objective: To observe the absolute, relative weight of kidney and body weightof albino rats on celecoxib induced kidney with protection by lycopene. Study Design:Experimental study. Place and Duration of study: This study was conducted in BMSI (Anatomydepartment), JPMC, Karachi, from 4th May 2015 to 3rd June 2015. Materials and Methods: Fortyhealthy adult, male Albino rats, 90-120 days old, weighing 200-220gm was taken for the study.The rats were divided into 4 groups, Group A was control group, Group B receive Celecoxib 50mg/kg body weight orally, Group C receive Celecoxib 50 mg/kg body weight orally along withlycopene50 mg/kg body weight orally and Group D receive lycopene 50 mg/kg body weightorally for 30 days. At the end of study rats were sacrificed and renal tissue sections were stainedwith hematoxylin and eosin. Results: Markedly decreased weight was observed in rats takingcelecoxib. Slides which were stained with hematoxylin and eosinshowed general architecture ofrenal parenchyma, shape and arrangement of epithelial cells. Apoptosis, hemorrhage, necrosisand vacuolation seen in Celecoxib group, whereas renal architecture were ameliorated andreverted back in celecoxib along with lycopene receiving group. Conclusion: This studyconcludes that lycopene restored the body weight, absolute and relative kidney weight incelecoxib treated group.

scholarly journals Evaluation of Chronic Administration of Metronidazole on the Morphological and Biochemical Parameters on the Cerebral Cortex of Adult Wistar Rats

2021 ◽  
pp. 31-43
Author(s):  
A. J. Ajibade ◽  
I .J. Ayanlade
Keyword(s):  
Cerebral Cortex ◽  
Wistar Rats ◽  
Biochemical Analysis ◽  
The Body ◽  
Degenerative Changes ◽  
Histological Techniques ◽  
Antibiotic Drug ◽  
Body Weights ◽  
Group A ◽  
Group B

Metronidazole has been implicated in diverse neurologic syndromes such as; cerebellar syndrome, encephalopathy, seizures, optic neuropathy, autonomic neuropathy and peripheral neuropathy.  Metronidazole is an antibiotic drug used to treat infections of the reproductive system, gastrointestinal tract, skin, heart, bone joint, lung, blood, nervous system and sexually transmitted diseases (STDs). This study therefore, investigated the effects of metronidazole on the cerebral cortex of adult wistar rats. Thirty-two (32) adult wistar rats of average weight of 180g of both sexes were distributed into four groups of eight (8) animals per group. Group A was the control group while group B, C and D rats were treated with 250 mg/kg, 500 mg/kg and 1000 mg/kg of metronidazole respectively. Metronidazole was administered orally on daily basis to the animals for 28 days. The weights of the rats were taken weekly using a weighing scale. On the 28th day of the treatment, the animals were sacrificed by cervical dislocation. The skulls were excised and the brains were harvested, weighed immediately using a sensitive weighing balance and then fixed in 10% formolcalcium for routine histological techniques and the other parts were processed for biochemical analysis of Malondialdehyde (MDA), Nitric Oxide (NO) and Succinate dehydrogenase (SDH). The results showed that there was a significant increase in the body weights of wistar rats in A and B while the mean body weights of the wistar rats reduced significantly in group C and D. The brain weights in group B and C increased insignificantly while brain weight in group D increased significantly when compared with group A. The biochemical analysis showed significant increase (P<0.05) in the level of MDA, NO and SDH in group B, C and D as compared with group A. Histological study of the cerebral cortex revealed conspicuous degenerative changes in group B while group C and D showed increased degenerative cerebral cortical layers with peripheral and central degenerative changes. The study concluded that metronidazole exhibited a neurodegenerative effect on the cerebral cortex of the wistar rats investigated. It is recommended that other studies should be carried out to corroborate these findings.

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