Growth hormone dependent human serum stimulation of thymidine and sulphate incorporation into embryonic chicken cartilage

1980 ◽  
Vol 94 (4) ◽  
pp. 480-488
Author(s):  
John Jennings ◽  
Fred Buchanan ◽  
Lynne Levitsky ◽  
John T. Garland
Keyword(s):  
Growth Hormone ◽  
Single Dose ◽  
Human Serum ◽  
Gh Deficiency ◽  
Serum Concentrations ◽  
Serum Stimulation ◽  
Embryonic Chicken ◽  
Normal Human ◽  
Normal Adults ◽  
Stimulation Of

Abstract. The embryonic chicken cartilage somatomedin bioassay was modified so that human serum stimulation of simultaneous [3H]methylthymidine and H2[35S]04 incorporation could be assessed. The assay consisted of a 6 h pre-incubation of 10 day pelvic rudiments in enriched buffer, followed by a 24 h incubation with buffer and low (0.5, 2 and 5% v/v) serum concentrations. Both labels were present for the final 6 h. Other modifications were shortening of washing, elimination of drying and weighing, and simplification of digestion. Normal human serum produced a linear log dose-response with these serum concentrations. Potency ratios in patients with GH deficiency were less than those of normal adults for both thymidine 0.39 ± 0.05 (mean ± sem, n = 16, range 0.22-0.71) vs. 0.90 ± 0.05 (n = 19, 0.62—1.36, P< 0.001) and for sulphate 0.40 ± 0.04 (0.15—0.65) vs. 94 ± 0.05 (0.61—1.29, P< 0.001). Potency ratios for both labels rose following administration of a single dose (0.2 IU/kg im) of hGH to 4 GH deficient children. The reliability of prediction of GH deficiency, reproducibility, and precision were similar to other Sm bioassays. The major advantages of these modifications were the ability to examine 2 cartilage metabolic processes simultaneously and the small amount of serum (350 μl) necessary for patient assays.

Growth Hormone-Dependent Serum Stimulation of DNA Synthesis in Chick Embryo Fibroblasts in Culture12

Endocrinology ◽  
1975 ◽  
Vol 96 (1) ◽  
pp. 193-198 ◽  
Author(s):  
KENNETH L. COHEN ◽  
PATRICIA A. SHORT ◽  
S. PETER NISSLEY
Keyword(s):  
Growth Hormone ◽  
Chick Embryo ◽  
Dna Synthesis ◽  
Serum Stimulation ◽  
Embryo Fibroblasts ◽  
Stimulation Of

FACTORS GOVERNING THE STIMULATION OF EMBRYONIC CHICK CARTILAGE BY SOMATOMEDIN

1976 ◽  
Vol 83 (2) ◽  
pp. 259-268 ◽  
Author(s):  
A. C Herington ◽  
L. S. Phillips ◽  
W. H. Daughaday
Keyword(s):  
Human Serum ◽  
Incubation Time ◽  
Dry Weight ◽  
Good Precision ◽  
Embryonic Chick ◽  
Prolonged Incubation ◽  
Significant Stimulation ◽  
Normal Human ◽  
Assay Precision ◽  
Stimulation Of

ABSTRACT In these studies of the stimulation of embryonic chick pelvic rudiments by somatomedin (Sm) in serum, we have found that cartilage weight and duration of cartilage exposure to serum determine the stimulation of cartilage by serum and the relative stimulation by Sm and by other non-Sm serum components, respectively. These factors are critical for the use of this system to measure Sm in serum. Initial experiments revealed that incorporation of sulfate (SO4) by cartilage incubated in buffer fell rapidly after 9 h and reached very low levels by 30 h. Incubation in 40 % normal human serum (NHS) produced significant stimulation of incorporation of SO4 after 4 h, and maintained at least initial levels of incorporation for 24 h. The greatest % stimulation by NHS over buffer was seen with prolonged incubation (44 h). However, specificity for Sm (discrimination between NHS and hypophysectomized human serum (HHS)) was greater with shorter incubation times. The potency of HHS was 11, 42 and 92 % of the potency of NHS following early, intermediate and late measurement of incorporation of SO4 by cartilage, respectively. The best overall results were obtained with intermediate incubation time and measurement of SO4 incorporation ((35S)-SO4 present for the final 5 h of a 25 h incubation), which allowed good precision (λ = 0.17) while maintaining satisfactory specificity for Sm. Since prolonged incubation with late measurement of SO4 incorporation allowed the greatest % stimulation by serum with little differentiation between NHS and HHS, stimulation of incorporation of SO4 under these conditions is apparently due to non-Sm factors present in both NHS and HHS. In addition to incubation time, cartilage stimulation by serum was also determined by cartilage weight. Lighter cartilage (from younger embryos) was associated with higher unstimulated incorporation of SO4 (P < 0.01), lower stimulation by added serum (P < 0.01), and inadequate assay precision (P < 0.05): satisfactory assays were generally obtained with cartilage rudiments weighing more than 0.7 mg (dry weight).

scholarly journals Growth hormone level during stimulation test and efficacy of growth hormone therapy of idiopathically undersized children with this hormone insufficiency

1993 ◽  
Vol 39 (5) ◽  
pp. 82-85
Author(s):  
M. B. Ranke ◽  
O. Gilbod
Keyword(s):  
Growth Hormone ◽  
Hormone Level ◽  
Gh Deficiency ◽  
Growth Hormone Level ◽  
Standard Samples ◽  
Results Of Treatment ◽  
Group A ◽  
Deficiency Group ◽  
First Time ◽  
Stimulation Of

For the first time, a broad study of the effectiveness of treatment with growth hormone (GH) in patients with varying degrees of impaired secretion of GH is undertaken. In this work, 885 patients of pre-pubertal age who received treatment for 1 year were examined. All children were given 2 standard tests with stimulation of the secretion of GH. The following diagnoses were established: idiopathic insufficiency of GH (classification number 1.1) or idiopathic stunting (3.1). Patients were divided into groups depending on the maximum release of GH during sampling: A - in both samples 5 ng/ml, B - in one sample 5 ng/ml, in another - 5-10 ng/ml, C - in both samples 5-10 ng/ml, D - in one sample 5-10 ng/ml, in another - 10 ng/ml, E - in both samples 10 ng/ml.anthropometric indicators, main parameters of therapy and results of treatment of GY were evaluated in all developed model (expected RESULTS). According to the study, patients with the most severe GH deficiency (group A) had better treatment outcomes, while the other groups were almost identical. The division of group A into a subgroup with isolated GH deficiency and a subgroup with deficiency of other tropic hormones (hypopituitarism) showed that in the first subgroup the growth of parents was significantly lower. Thus, despite the fact that standard samples with stimulation of GH can be used to diagnose the insufficiency of GH, the results of these samples do not always allow to predict the effectiveness of treatment of GH for 1 year. It should also be noted that the shortness of the parents, especially the growth of the mother, may indicate the presence of an additional hereditary factor in the Genesis of shortness in these patients.

Measurement of nocturnal urinary growth hormone values

1989 ◽  
Vol 121 (2) ◽  
pp. 290-296 ◽  
Author(s):  
Izumi Sukegawa ◽  
Naomi Hizuka ◽  
Kazue Takano ◽  
Kumiko Asakawa ◽  
Reiko Horikawa ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Gh Deficiency ◽  
Turner's Syndrome ◽  
Turner’S Syndrome ◽  
Gh Secretion ◽  
Short Children ◽  
The Mean ◽  
Plasma Gh ◽  
Normal Adults ◽  
Collection Time

Abstract. Nocturnal urinary growth hormone values were measured by a sensitive enzyme immunoassay in normal adults, patients with GH deficiency, patients with Turner's syndrome, normal but short children who had normal plasma GH responses to provocative tests, and patients with acromegaly. The mean nocturnal urinary GH values in patients with acromegaly were significantly greater than those in normal adults (1582.3 ± 579.8 vs 53.5 ± 8.6 pmol/mmol creatinine (± sem); p < 0.05). In the normal but short children and patients with Turner's syndrome, the mean nocturnal urinary GH values were 83.1 ± 5.2 and 79.8 ± 29.5 pmol/mmol creatinine, respectively. In patients with GH deficiency, the nocturnal urinary GH values were undetectable (< 5.3 pmol/mmol creatinine) except in one patient where the value was 6.3 pmol/mmol creatinine. The nocturnal urinary GH values of the patients with GH deficiency were significantly lower than those of the other groups (p < 0.05). In normal but short children, the nocturnal urinary GH values correlated significantly with mean plasma nocturnal GH concentrations (r = 0.76, p < 0.001), and 24-hour urinary GH values (r = 0.84, p < 0.001), respectively. In 4 patients with GH deficiency who had circulating anti-hGH antibody, the urinary GH values were also undectable. These data indicate that nocturnal urinary GH value reflects endogenous GH secretion during collection time, and that measurement of the nocturnal urinary GH values is a useful method for screening of patients with GH deficiency and acromegaly.

Stimulation of release of growth hormone from the anterior pituitary by α-melanocyte-stimulating hormone: a possible mechanism for induction of pseudopregnancy in the rat

1988 ◽  
Vol 118 (3) ◽  
pp. 465-470
Author(s):  
M. Volosin ◽  
M. E. Celis
Keyword(s):  
Growth Hormone ◽  
Continuous Infusion ◽  
Anterior Pituitary ◽  
Significant Rise ◽  
The Other ◽  
Serum Concentrations ◽  
Melanocyte Stimulating Hormone ◽  
Hypophysectomized Rats ◽  
Stimulation Of ◽  
Intact Rats

ABSTRACT In hypophysectomized rats on day 1 of dioestrus, as well as on day 4 of pseudopregnancy, α-MSH (continuous infusion of 1 μg/h) failed to maintain serum concentrations of progesterone. On the other hand, α-MSH did not modify the increase induced by ACTH (1 μg/μl as an infusion plus two additional daily injections of 30 μg/μl), prolactin (200 μg/0·2 ml at 12-h intervals) or GH (300 μg/0·2 ml twice daily) on serum concentrations of progesterone in such rats. However, in intact rats α-MSH caused a significant rise in serum concentrations of GH on day 1 and day 2 of dioestrus. Continuous infusion of α-MSH produced an increase in serum concentrations of GH at 12.00 and 14.00 h on day 1 of dioestrus and at 07.00 h on day 2. It is therefore suggested that α-MSH may exert its effect by facilitating the secretion of GH, which in turn may induce the release of progesterone. J. Endocr. (1988) 118, 465–470

scholarly journals Serum Concentrations of 20K Human Growth Hormone in Normal Adults and Patients with Various Endocrine Disorders

2000 ◽  
Vol 47 (SupplMarch) ◽  
pp. S17-S21 ◽  
Author(s):  
TOSHIO TSUSHIMA ◽  
YUZURU KATOH ◽  
YUKITAKA MIYACHI ◽  
KAZUO CHIHARA ◽  
AKIRA TERAMOTO ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Human Growth ◽  
Endocrine Disorders ◽  
Serum Concentrations ◽  
Normal Adults

EFFECT OF THE STRESS OF INJECTIONS ON THE SERUM CONCENTRATION OF CORTISOL, PROLACTIN, AND GROWTH HORMONE IN GILTS AND LACTATING SOWS

1989 ◽  
Vol 69 (3) ◽  
pp. 663-672 ◽  
Author(s):  
S. ROBERT ◽  
A.-M. B. DE PASSILLÉ ◽  
N. ST-PIERRE ◽  
G. PELLETIER ◽  
D. PETITCLERC ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Physical Restraint ◽  
Latin Square ◽  
Serum Cortisol ◽  
Blood Collection ◽  
Serum Concentrations ◽  
Subcutaneous Injections ◽  
Blood Samples ◽  
Lactating Sows ◽  
Stimulation Of

Three experiments were conducted to determine the effect of subcutaneous injections of saline on serum concentrations of cortisol, prolactin and growth hormone in cycling gilts and lactating sows. In exp. 1, five crossbred cycling gilts were given one subcutaneous injection and blood was sampled during the preceding and following four hours. In exp. 2, blood samples were taken from six purebred lactating sows on a control day and on a day when they were injected with saline in a latin square design. Serum concentrations of cortisol, prolactin and growth hormone were monitored in exps. 1 and 2. In exp. 3, 11 purebred primiparous sows received subcutaneous injections twice daily from 5 to 25 d postpartum. Blood samples were collected for cortisol assay on days 5, 15 and 25. Serum cortisol concentrations increased significantly after saline injection in the three experiments, confirming that injections cause a stimulation of the hypothalamic-hypophyseal-adrenal axis in cycling gilts and lactating sows. When additional stressors such as handling and physical restraint were imposed prior to the injection, a release of prolactin and growth hormone was also observed in cycling gilts. The serum cortisol profiles of lactating sows repeatedly injected showed that their cortisol response to subcutaneous injections attenuated over days. Furthermore, these data indicate that the animals became readily acclimated to blood collection from a catheter within the first day of sampling. Key words: Cortisol, prolactin, growth hormone, stress, gilts, lactating sows

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