Red-cell 2,3-diphosphoglycerate in patients with hyperthyroidism

1980 ◽  
Vol 93 (4) ◽  
pp. 424-429 ◽  
Author(s):  
J. L. Alvarez-Sala ◽  
M. A. Urbán ◽  
J. J. Sicilia ◽  
A. J. Diaz Fdez ◽  
F. Fdez Mendieta ◽  
...  
Keyword(s):  
Thyroid Hormones ◽  
Control Group ◽  
Red Cell ◽  
Oxygen Dissociation Curve ◽  
Dissociation Curve ◽  
Oxygen Dissociation ◽  
The Right ◽  
Serum Thyroid Hormones ◽  
Hyperthyroid Patients ◽  
2,3 Dpg

Abstract. In 21 hyperthyroid female patients studied on 29 occasions, high levels of red-cell 2,3-diphosphoglycerate (2,3-DPG) have been found (5.75 ± 0.7 mm) which, compared to a euthyroid control group (4.88 ± 0.4 mm), could not be accounted for by differences in haematocrit, haemoglobin or phosphataemia. A significant correlation was found (P < 0.05) between serum thyroid hormones and the 2,3-DPG concentration in the hyperthyroid patients. Eight of these patients were reexamined after treatment and normalization of thyroid function, showed a regression to normal 2,3-DPG values (4.81 ± 0.6 mm) which could not be attributed to variations in haematocrit, haemoglobin or phosphataemia either. We therefore deduce that the shift to the right in the haemoglobin oxygen dissociation curve observed in patients of this type may be due to an increase in the red-cell 2,3-DPG content.

The Bohr effect related to blood and erythrocyte pH

1963 ◽  
Vol 205 (2) ◽  
pp. 337-340 ◽  
Author(s):  
Peter Hilpert ◽  
Renate Gislinde Fleischmann ◽  
Doris Kempe ◽  
Heinz Bartels
Keyword(s):  
Oxygen Pressure ◽  
Ph Value ◽  
Oxygen Affinity ◽  
Bohr Effect ◽  
Red Cell ◽  
Oxygen Dissociation Curve ◽  
Dissociation Curve ◽  
Oxygen Dissociation ◽  
Constant Cell ◽  
The Right

The Bohr effect of the blood and the red cell hemolysates of adult and newborn humans, goats and kids, and sheep and lambs were determined and the physiological significance is discussed. Similar determinations were made on blood from an African elephant, yak, camel, Dybowski deer, and llama. The strong displacement to the right of the oxygen dissociation curve which occurs in kids and lambs during the first 5 days of life can be largely explained by a change in the pH value within the erythrocytes. When the oxygen affinity is expressed by the oxygen pressure necessary for half saturation (T50) at a constant cell pH, considerable differences exist between species.

The Lack of Effects of Phototherapy on Neonatal Oxygen Dissociation Curves and Hemoglobin Concentration in Vivo

PEDIATRICS ◽  
1977 ◽  
Vol 59 (6) ◽  
pp. 1027-1031
Author(s):  
Barry D. Chandler ◽  
William J. Cashore ◽  
Pierre J. P. Monin ◽  
William Oh
Keyword(s):  
Serum Bilirubin ◽  
Hemoglobin Concentration ◽  
Control Group ◽  
Dissociation Curve ◽  
Oxygen Dissociation ◽  
The Right ◽  
Dissociation Curves ◽  
2,3 Dpg

Phototherapy has been shown to cause hemolysis of fetal red cells and a shift to the right in the neonatal oxygen dissociation curve (ΔP50) in vitro. To determine if these parameters act similarly in vivo, we have studied 16 icteric infants before and after phototherapy and compared them with eight control infants studied at birth and at 3 days of age, measuring the change in P50, 2,3-diphosphoglycerate (2,3-DPG), serum bilirubin, the percent of bilirubin/albumin saturation, and the hemoglobin concentration in the two groups. Following phototherapy, in the icteric infants there was a shift to the right in the O2 dissociation curve of + 1.7 mm Hg, a rise in 2,3-DPG of 2.26 µmol/gm Hb, a fall in serum bilirubin of 4.3 mg/100 ml, a decrease in percent bilirubin/albumin saturation of 12.4%, and a drop in hemoglobin of 1.1 gm/100 ml. The control group showed a ΔP50 of + 2.0 mm Hg, a rise in 2,3-DPG of 3.67 µmol/gm Hb, an increase in serum bilirubin of 3.2 mg/100 ml, an increase in percent bilirubin/albumin saturation of 9.3%, and a fall in hemoglobin of 0.3 gm/100 ml. Significant differences between the groups were seen only in the changes in bilirubin concentration and percent bilirubin/albunmin saturation. The magnitude of changes in P50, 2,3-DPG, and hemoglobin concentration was similar in the phototherapy and control groups and was related to the expected changes with reference to postnatal age. These results suggest that phototherapy in vivo neither affects fetal erythrocytic affinity for oxygen nor causes hemolysis.

scholarly journals A Study of the Sickling Phenomenon and Oxygen Dissociation Curve in Patients with Hemoglobins SS, SD, SF and SC

Blood ◽  
1969 ◽  
Vol 34 (5) ◽  
pp. 682-690 ◽  
Author(s):  
M. J. CAWEIN ◽  
R. P. O’NEILL ◽  
L. A. DANZER ◽  
E. J. LAPPAT ◽  
THOMAS ROACH
Keyword(s):  
Venous Blood ◽  
Cell Formation ◽  
Oxygen Dissociation Curve ◽  
Normal Curve ◽  
Dissociation Curve ◽  
Sickle Cells ◽  
Oxygen Dissociation ◽  
Effect Of Therapy ◽  
The Right ◽  
Dissociation Curves

Abstract 1. Data on pO2, T50, n values and per cent of sickle cells were obtained in patients with hemoglobins SS, SD, SF (S-thalassemia) and SC. In general the per cent of sickle cells was proportional to the per cent of hemoglobin S. The number of sickle cells was, however, influenced by the other type of hemoglobin present and by the pO2. Values for T50 were greater than normal, and values for n were less than normal. 2. The effect of therapy with O2, CO2, NaHCO3 and MgSO4 on the per cent of sickle cells in arterial and venous blood was determined. Only inhalation of O2 reduced the per cent of sickle cells. 3. The rate of sickle cell formation and its reversal were determined under conditions of deoxygenation and oxygenation. These were found to be a function of the type of hemoglobin combinations present. 4. The oxygen dissociation curves were found to be shifted to the right of the normal curve indicating a decreased affinity for oxygen. In two patients the Bohr effect was determined and found to be normal.

Chloride and inorganic phosphate modulate binding of oxygen to bovine red blood cells

1994 ◽  
Vol 77 (1) ◽  
pp. 202-208 ◽  
Author(s):  
P. Gustin ◽  
B. Detry ◽  
M. L. Cao ◽  
F. Chenut ◽  
A. Robert ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Oxygen Affinity ◽  
Oxygen Binding ◽  
Oxygen Dissociation Curve ◽  
Dissociation Curve ◽  
Oxygen Dissociation ◽  
Hemoglobin Saturation ◽  
The Difference ◽  
The Right

The influence of Pi and Cl on the equilibrium of oxygen binding to bovine red blood cells was assessed by plotting the whole blood oxygen dissociation curve measured under standard conditions with and without added KCl and K2HPO4. Both salts shifted the oxygen dissociation curve to the right. This effect was more marked at the highest saturation levels. At a given saturation level, the anion-induced shift was linearly related to the concentration of salt added to the blood. Cl had a greater effect than Pi. The relationship between changes in Po2 at 50% hemoglobin saturation (in Torr) and concentrations of ions added (in mmol/l) was equal to 0.0515[Cl] + 0.0302[Pi] (r2 = 0.94; P < 0.001). These changes were not due to the hyperosmolality induced by salt addition, since sucrose added in place of salts was without effect on the measured parameters. The oxygen exchange fraction expressed as percentage of saturation, i.e., the difference in hemoglobin saturation when Po2 decreases from 130 to 40 Torr, was linearly correlated to added anion concentration (in mmol/l) (= 0.102[Cl] + 0.059[Pi] (r2 = 0.95; P < 0.001)). No significant interaction between the anions was observed; their effects were purely additive. This original mechanism of controlling the oxygen affinity of bovine blood may have clinical relevance: Cl and Pi could be used to increase oxygen transport efficiency in hypoxic animals.

Alzheimer’s Disease: Erythrocyte 2,3-diphosphoglycerate Content and Circulating Erythropoietin

2019 ◽  
Vol 16 (9) ◽  
pp. 834-835
Author(s):  
Petter Järemo ◽  
Alenka Jejcic ◽  
Vesna Jelic ◽  
Tasmin Shahnaz ◽  
Homira Behbahani ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Cognitive Impairment ◽  
Cell Damage ◽  
Control Group ◽  
Red Cell ◽  
Oxygen Release ◽  
Regulatory Pathways ◽  
Β Amyloid ◽  
2,3 Dpg

Background: Alzheimer’s Disease (AD) features the accumulation of β-amyloid in erythrocytes. The subsequent red cell damage may well affect their oxygen-carrying capabilities. 2,3- diphosphoglycerate (2,3-DPG) binds to the hemoglobin thereby promoting oxygen release. It is theorized that 2,3-DPG is reduced in AD and that the resulting hypoxia triggers erythropoietin (EPO) release. Methods & Objective: To explore this theory, we analyzed red cell 2,3-DPG content and EPO in AD, mild cognitive impairment, and the control group, subjective cognitive impairment. Results: We studied (i) 2,3-DPG in red cells, and (ii) circulating EPO in AD, and both markers were unaffected by dementia. Disturbances of these oxygen-regulatory pathways do not appear to participate in brain hypoxia in AD.

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