RESPONSES OF SERUM GONADOTROPHINS TO LH-RELEASING HORMONE AND OESTROGENS IN JAPANESE WOMEN WITH POLYCYSTIC OVARIES

1977 ◽  
Vol 85 (4) ◽  
pp. 840-849 ◽  
Author(s):  
Toshihiro Aono ◽  
Masatoshi Miyazaki ◽  
Akira Miyake ◽  
Takayuki Kinugasa ◽  
Keiichi Kurachi ◽  
...  
Keyword(s):  
Ovarian Response ◽  
Japanese Patients ◽  
Japanese Women ◽  
Polycystic Ovaries ◽  
Releasing Hormone ◽  
Gonadotrophin Secretion ◽  
The Mean ◽  
Lh Rh ◽  
Lh Releasing Hormone ◽  
Normal Controls

ABSTRACT In order to define the abnormality in gonadotrophin secretion in Japanese women with polycystic ovaries (PCO) who rarely show virilization and markedly enlarged ovaries, basal levels of LH and FSH, and responses of serum gonadotrophins to LH-releasing hormone (LH-RH) or oestrogens were determined by radioimmunoassay. Eleven patients with PCO diagnosed by laparotomy or laparoscopy and 30 normal women in the follicular phase were studied. The mean (± sd) basal level of LH was significantly higher in patients with PCO than in normal controls (PCO 28.6 ± 2.4 vs. normal 10.9 ± 3.0 mIU/ml), while the mean FSH level in PCO patients was not significantly different from that in the normal controls (9.7 ± 0.7 vs. 11.4 ± 2.6 mIU/ml). The mean LH/FSH ratio in PCO patients was significantly higher than that in normal controls (3.2 ± 0.9 vs. 1.0 ± 0.3). Exaggerated response of LH to LH-RH was observed in PCO patients, while the FSH response was comparable with the normal controls. Ten out of 11 patients with PCO showed LH release exceeding the basal level after bolus iv injection of 20 mg conjugated oestrogens (Premarin®), and virtually the same mean net increase in LH from the basal level was obtained in both PCO patients and normal controls. Since the abnormalities in gonadotrophin secretion in Japanese women with PCO are not different from those reported in patients with PCO in Europe and USA, it seems likely that lower incidence of markedly enlarged ovaries and virilization in Japanese patients may be caused by the difference in ovarian response to gonadotrophin.

PLASMA TESTOSTERONE AND URINARY STEROIDS IN JAPANESE WOMEN WITH POLYCYSTIC OVARIES

1971 ◽  
Vol 68 (2) ◽  
pp. 293-302 ◽  
Author(s):  
K. Kurachi ◽  
M. Miyazaki ◽  
S. Mizutani ◽  
K. Matsumoto
Keyword(s):  
Urinary Metabolites ◽  
Japanese Patients ◽  
Japanese Women ◽  
Plasma Testosterone ◽  
Polycystic Ovaries ◽  
Before And After ◽  
Human Menopausal Gonadotrophin ◽  
Normal Women ◽  
The One ◽  
Normal Controls

ABSTRACT Urinary metabolites of cortisol, 11-oxy-17-ketosteroids, 11-deoxy-17-ketosteroids and oestrogens were measured in 8 normal Japanese women and 21 Japanese patients with polycystic ovaries, both before and after dexamethasone administration or ovarian stimulation with human menopausal gonadotrophin under adrenal suppression. Plasma testosterone was estimated in 12 normal women and also in 9 of the patients. It has been suggested that polycystic ovaries secrete significantly larger quantities of androgens than normal ovaries, even though very few Japanese women with polycystic ovaries show any signs of hirsutism. Although the patients had higher mean plasma testosterone value (58 ± 35 (sd) ng/100 ml) than the normal controls (30 ± 15 (sd) ng/100 ml), only 2 of the 9 patients had plasma testosterone values higher than the normal range. Of the 9 patients, hirsutism was found in the one who showed the highest value (141 ng/100). No evidence of the failure of oestrogen formation from androgens was obtained in Japanese women with polycystic ovaries.

PHOTOPERIODIC CONTROL OF REPRODUCTION IN JAPANESE QUAIL: CHANGES IN GONADOTROPHIN SECRETION ON THE FIRST DAY OF INDUCTION AND THEIR PHARMACOLOGICAL BLOCKADE

1977 ◽  
Vol 74 (3) ◽  
pp. 449-460 ◽  
Author(s):  
B. K. FOLLETT ◽  
D. T. DAVIES ◽  
B. GLEDHILL
Keyword(s):  
Pituitary Gland ◽  
Japanese Quail ◽  
Continuous Light ◽  
Sensitive Period ◽  
Blood Samples ◽  
Long Day ◽  
Gonadotrophin Secretion ◽  
The Mean ◽  
Lh Rh ◽  
Lh Releasing Hormone

SUMMARY Photoperiodic processes often seem rather slow because the induced developmental changes, such as gonadal growth in a seasonally breeding bird, may take many weeks to reach completion. This is rather deceptive, however, as initial changes occur rapidly. Following the transfer of sexually immature Japanese quail to a photoperiod of 20 h light:4 h darkness (20L:4D), plasma levels of both LH and FSH remained low on the first day of treatment until 18 h after the onset of light when they both rose significantly. Over the next few hours the levels of both hormones rose three- to fourfold. In a more detailed experiment in which blood samples were collected every 2 h, the mean LH concentration showed no increase before hour 20 but rose significantly from 0·92± 0·09 (s.e.m.) to 1·67± 0·19 ng/ml (n = 6) between hours 20 and 22. By hour 26 the mean LH level had reached 2·68 ± 0·25 ng/ml. To determine the light-sensitive period on the first day of photostimulation, immature quail were transferred from short days (8L: 16D) to one of the following photoperiods: 12L: 12D, 13·3L: 10·7D, 14·7L: 9·3D, 16L: 8D, 20L: 4D or to continuous light. Blood samples were collected at hours 12, 19 and 25 after the onset of light. The concentration of LH did not change in the groups exposed to 12L:12D or to 13·3L:10·7D. In all other groups, however, it increased significantly between hours 19 and 25 regardless of whether the quail were in darkness or light, or whether a light/dark transition occurred. The magnitude of the rise was similar in the four stimulatory photoperiods. These results indicate that light before the twelfth hour is not photoperiodically effective but that further exposure to 3 h or more light from hour 12 causes induction after a lag of some hours, and an increase in LH at the end of the first day. When the LH rise was observed over 2 consecutive long days the response seemed to occur earlier on the second than on the first day. To see whether the delay in the rise of LH secretion until hour 20 was due to a change in the sensitivity of the pituitary gland to LH releasing hormone (LH-RH), birds were tested in three experiments. 'Short-day' quail were injected i.m. with two equal doses of synthetic LH-RH, the second dose being given 30, 60 or 90 min after the first. The results indicated that the first dose of LH-RH did not enhance the amount of LH released by the second dose. In fact, the response to the second dose was significantly less. Similarly, an injection of LH-RH at hour 12 did not advance the LH response on the first long day. Finally, the sensitivity of the pituitary gland to exogenous LH-RH was determined at various times on the first long day. No augmented responsiveness was observed at hours 22 and 24. It would appear, therefore, that the lag may not be in the pituitary gland but may exist in neural or neuroendocrine events which occur in the brain of the quail between hours 12 and 16 and which lead to enhanced gonadotrophin secretion beginning at about hour 20 on the first long day.

GONADOTROPHIN REGULATION IN THE LACTATING RAT

1978 ◽  
Vol 88 (3) ◽  
pp. 668-675 ◽  
Author(s):  
R. W. Steger ◽  
J. J. Peluso
Keyword(s):  
Positive Feedback ◽  
Post Partum ◽  
Ovariectomized Rats ◽  
Releasing Hormone ◽  
Gonadotrophin Secretion ◽  
Lh Release ◽  
Lh Rh ◽  
Lh Releasing Hormone

ABSTRACT Post-partum lactation in the rat is associated with follicular quiescence and an attenuation of gonadotrophin secretion. The present study demonstrates that the lactating rat responds to exogenous LH-releasing hormone (LH-RH) in a manner similar to dioestrous rats. Oestrogen priming increases the LH and FSH response to LH-RH to a smaller degree in ovariectomized, lactating, than in non-lactating, ovariectomized rats. Pituitary LH levels throughout lactation did not seem to be related to LH-RH-induced LH release. A diminished post-castration rise in both LH and FSH, and a diminished positive feedback response to oestrogen administration were also observed and may indicate a disruption of gonadotrophin regulation at both the hypothalamic and the pituitary level.

USE OF A PULSED INFUSION OF LUTEINIZING HORMONE RELEASING HORMONE TO MIMIC SEASONALLY INDUCED ENDOCRINE CHANGES IN THE RAM

1979 ◽  
Vol 83 (2) ◽  
pp. 251-260 ◽  
Author(s):  
G. A. LINCOLN
Keyword(s):  
Plasma Concentrations ◽  
Prolonged Treatment ◽  
Mating Season ◽  
Releasing Hormone ◽  
Natural Lighting ◽  
Sexual Skin ◽  
Gonadotrophin Secretion ◽  
The Individual ◽  
Lh Rh ◽  
Lh Releasing Hormone

Adult Soay rams were housed indoors under natural lighting during the spring non-mating season when gonadotrophin secretion was low. Four animals received small doses (100 ng or 500 ng) of synthetic LH releasing hormone (LH-RH) infused into the jugular vein by a mechanical device for 60 s every 2 h for 33–57 days: two other rams acted as controls. The prolonged treatment with LH-RH resulted in growth of the testes and the development of the sexual skin flush; these effects were lost when treatment stopped. The plasma concentrations of LH, FSH and testosterone were low at the beginning; each short infusion of LH-RH resulted in a transitory increase in the level of LH and testosterone while the concentration of FSH was only marginally affected. After prolonged treatment with 500 ng pulses of LH-RH the plasma concentrations of all three hormones were permanently raised. The response to the individual injections of LH-RH was also modified, the peak in LH being reduced in amplitude but more prolonged while the FSH and testosterone responses were both enhanced. When the pulsed infusion was stopped the concentration of LH and testosterone declined rapidly while the decline in FSH levels took many days. These endocrine changes induced by the pulsed infusion are comparable to those that occur naturally in the ram during testicular redevelopment before the mating season.

EFFECT OF CORTISOL OR ADRENOCORTICOTROPHIN ON RELEASE OF LUTEINIZING HORMONE INDUCED BY LUTEINIZING HORMONE RELEASING HORMONE IN THE DAIRY HEIFER

1982 ◽  
Vol 92 (1) ◽  
pp. 141-146 ◽  
Author(s):  
R. L. MATTERI ◽  
G. P. MOBERG
Keyword(s):  
Luteinizing Hormone ◽  
Luteinizing Hormone Releasing Hormone ◽  
Releasing Hormone ◽  
Dairy Heifers ◽  
Significant Difference ◽  
Significant Depression ◽  
Lh Rh ◽  
Lh Releasing Hormone ◽  
And Control ◽  
Slight Depression

During treatment with cortisol or ACTH, dairy heifers were given two doses of LH releasing hormone (LH-RH) spaced 1·5 h apart. Serum concentrations of cortisol and LH were monitored during each treatment. Treatment with both ACTH and cortisol raised plasma cortisol levels above the respective saline controls (P<0·001). Neither treatment affected basal LH concentrations. A slight depression in LH response was seen in the cortisol-treated animals after the first LH-RH injection, as shown by a statistically significant depression at three of the sample times. There was no significant difference between treated and control LH values after the second LH-RH administration. Treatment with ACTH resulted in significantly reduced LH values at all sample times after both injections of LH-RH.

Relative Insensitivity of Rhesus Monkeys to the LH-Releasing Hormone (LH-RH)

1973 ◽  
Vol 36 (2) ◽  
pp. 372-374 ◽  
Author(s):  
AKIRA ARIMURA ◽  
HAROLD G. SPIES ◽  
ANDREW V. SCHALLY
Keyword(s):  
Rhesus Monkeys ◽  
Releasing Hormone ◽  
Lh Rh ◽  
Lh Releasing Hormone

DIAGNOSTIC VALUE OF THE LH-RELEASING HORMONE STIMULATION TEST IN FUNCTIONAL AMENORRHEA

1975 ◽  
Vol 78 (4) ◽  
pp. 625-633 ◽  
Author(s):  
D. Vandekerckhove ◽  
M. Dhont ◽  
J. Van Eyck
Keyword(s):  
Anorexia Nervosa ◽  
Diagnostic Value ◽  
Base Line ◽  
List Type ◽  
Releasing Hormone ◽  
The Absolute ◽  
Lh Rh ◽  
Lh Releasing Hormone ◽  
Circulating Levels ◽  
Secretory Capacity

ABSTRACT LH-releasing hormone (25 μg, iv.) was administered to 37 women with functional amenorrhea. In addition to the clinical classification, these patients were divided into three groups according to the basal level of serum LH. A significant correlation was found between the base-line levels of LH and the serum concentration of oestradiol plus oestrone. The absolute increment of LH after the injection of LH-RH was found to be dependent only on the base-line level of LH. Except for the patients with anorexia nervosa, the base-line levels and the response pattern of FSH were almost the same for all three groups. From the results of this study, it was concluded that: The circulating levels of oestradiol and oestrone, where derived from ovarian secretion, actually depend on the gonadotrophic stimulus. In patients with functional amenorrhea, the oestrogens do not make an independent contribution to the pituitary response to LH-RH. Dysregulation of releasing hormones, whether located at the hypothalamic or suprahypothalamic level, necessarily influences the secretory capacity of the pituitary gland; long-standing deficiency of LH-RH may finally lead to a state of pituitary "functional" unresponsiveness to releasing hormones. In view of the excellent correlation between the base-line levels of LH and the absolute increment of LH following stimulation with LH-RH, this test only accentuates the existing pituitary secretory capacity, which can be roughly estimated from the circulating levels of LH and FSH. This test may be useful in distinguishing the milder cases of psychogenic amenorrhea from extreme gonadotrophic dysfunction in patients with anorexia nervosa.

RESTORATION OF OESTROGEN POSITIVE FEEDBACK EFFECT ON LH RELEASE BY BROMOCRIPTINE IN HYPERPROLACTINAEMIC PATIENTS WITH GALACTORRHOEA-AMENORRHOEA

1979 ◽  
Vol 91 (3) ◽  
pp. 591-600 ◽  
Author(s):  
Toshihiro Aono ◽  
Akira Miyake ◽  
Takenori Shioji Motoi Yasuda ◽  
Koji Koike ◽  
Keiichi Kurachi
Keyword(s):  
Luteinizing Hormone ◽  
Follicle Stimulating Hormone ◽  
Serum Prolactin ◽  
Serum Luteinizing Hormone ◽  
Serum Lh ◽  
Lh Release ◽  
The Mean ◽  
Lh Rh ◽  
Lh Releasing Hormone ◽  
Positive Feedback Effect

ABSTRACT Five mg of bromocriptine was administered for 3 weeks to 8 hyperprolactinaemic women with galactorrhoea-amernorrhoea, in whom the response of serum luteinizing hormone (LH) and follicle-stimulating hormone (FSH) to 100 μg of iv LH-releasing hormone (LH-RH) had been evaluated. Twenty mg of conjugated oestrogen (Premarin®) was injected iv any day between the 10th and 12th day from the initiation of the treatment, and serum LH levels were serially determined for 120 h. Hyperresponse of LH with normal FSH response to LH-RH was observed in most patients. Bromocriptine treatment for 10 to 12 days significantly suppressed mean (± se) serum prolactin (PRL) levels from 65.1 ± 23.0 to 10.4 ± 2.0 ng/ml, while LH (12.6 ± 2.1 to 24.8 ± 5.9 mIU/ml) and oestradiol (40.1 ± 7.6 to 111.4 ± 20.8 pg/ml) levels increased significantly. Patients on bromocriptine treatment showed LH release with a peak at 48 h after the injection of Premarin. The mean per cent increases in LH were significantly higher than those in untreated patients with galactorrhoea-amenorrhoea between 32 and 96 h after the injection. The present results seem to suggest that the restoration of LH-releasing response to oestrogen following suppression of PRL by bromocriptine may play an important role in induction of ovulation in hyperprolactinaemic patients with galactorrhoea-amenorrhoea.

scholarly journals Studies on the Measurements of LH-releasing Hormone (LH-RH) by Radioimmunoassay (RIA)

1976 ◽  
Vol 52 (1) ◽  
pp. 62-71
Author(s):  
Takayuki KTNUGASA ◽  
Osamu TANIZAWA ◽  
Kenji YAMAJI ◽  
Keiichi KURACHI
Keyword(s):  
Releasing Hormone ◽  
Lh Rh ◽  
Lh Releasing Hormone
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