RAT ENDOMETRIUM ENZYMES IN 4-DAY OESTROUS CYCLE AND EARLY PREGNANCY

1977 ◽  
Vol 85 (1) ◽  
pp. 169-176 ◽  
Author(s):  
Jan Jelínek ◽  
Marta Jelínková
Keyword(s):  
Alkaline Phosphatase ◽  
Acid Phosphatase ◽  
Lactate Dehydrogenase ◽  
Early Pregnancy ◽  
Enzyme Activities ◽  
Oestrous Cycle ◽  
The Other ◽  
Endogenous Hormones ◽  
Specific Activities ◽  
The Individual

ABSTRACT The specific activities of lactate dehydrogenase (LDH) and its M-type (M-LDH), β-glucuronidase (β-GR), acid phosphatase (ACP) and alkaline phosphatase (AP) were determined. The specific activities of the enzymes (LDH, β-GR) in the myometrium were lower and their changes less pronounced than in the endometrium. We, therefore, determined the enzymes in the rat endometrium only in further experiments. All enzymes react sensitively to the changes induced in the endometrium by endogenous hormones in the course of a 4-day cycle: pro-oestrus (P) is characterized by rather low enzyme activities, oestrus (E) by a peak of LDH and M-LDH and a rise of AP. In metoestrus (M) there is a peak of β-GR, ACP and AP. Dioestrus (D) is characterized by a significant decrease in LDH and M-LDH and by elevated values of all the other enzymes. The values on the individual days of the 4–day cycle were compared with days 4–6 of pregnancy. The reason for this was that if the rats were not mated, they would, respectively, return to pro-oestrus instead of being 4 days pregnant, to oestrus instead of being 5 days pregnant, or to metoestrus instead of being 6 days pregnant. We found the following differences: on day 4 of pregnancy LDH and M-LDH were lower and ACP and AP higher than in P. On day 5 of pregnancy the LDH, M-LDH, β-GR and AP were lower than in E On day 6 of pregnancy the LDH, M-LDH, ACP and especially β-GR, were lower than in M.

Lysosomal Enzyme Activities in Platelets of Normal Individuals and of Patients with Gaucher’s Disease

1974 ◽  
Vol 32 (02/03) ◽  
pp. 665-669 ◽  
Author(s):  
S Yatziv ◽  
M White ◽  
A Eldor
Keyword(s):  
Acid Phosphatase ◽  
Lysosomal Enzyme ◽  
Enzyme Activities ◽  
The Other ◽  
Gaucher's Disease ◽  
Gaucher’S Disease ◽  
Other Hand ◽  
Normal Individuals ◽  
Specific Activities ◽  
Normal Controls

SummaryFive platelet lysosomal enzyme activities were estimated in 22 normal individuals and in 5 patients with Gaucher’s disease: ß-D-galactosidase, N-Acetyl-ß-D-glucosa-minidase, N-Acetyl-ß-D-galactosaminidase acid-phosphatase, and ß-D-glucuronidase.In the 5 patients with Gaucher’s disease the specific activities for the first four of these enzymes were significantly higher than in normal individuals. The activity of acid-phosphatase, on the other hand, was lower in G. d. than in normals. The release of the 5 lysosomal enzymes from platelets upon incubation with thrombin was also examined in the same two groups. The only lysosomal enzyme that was not released from normal platelets was acid-phosphatase. In Gaucher’s patients, on the other hand, acid-phosphatase was invariably released from platelets when incubated with thrombin. The release of the other four enzymes from platelets of Gaucher patients was similar to that of normal controls.

scholarly journals BETA GLUCURONIDASE-RICH CYTOPLASMIC PARTICLES IN ANDROGEN-STIMULATED MOUSE KIDNEY

1963 ◽  
Vol 16 (2) ◽  
pp. 253-258 ◽  
Author(s):  
Andrew G. Plaut ◽  
William H. Fishman
Keyword(s):  
Alkaline Phosphatase ◽  
Acid Phosphatase ◽  
Specific Activity ◽  
Mouse Kidney ◽  
The Other ◽  
Mouse Testis ◽  
Glucuronidase Activity ◽  
Other Hand ◽  
Fraction I ◽  
Gonadotropic Hormones

Androgens produced by stimulating mouse testis with gonadotropic hormones cause a rise in renal ß-glucuronidase but not an increase in acid or alkaline phosphatase. All subcellular components increase in ß-glucuronidase activity, with a relatively greater increment in particulate enzyme as compared with that free in the cytoplasm (non-sedimentable). A small percentage of recovered ß-glucuronidase, acid phosphatase, and alkaline phosphatase is found in material which rises to the surface during centrifugation in sucrose media (fraction I). The specific activity of ß-glucuronidase and acid phosphatase in this fraction is normally quite high with respect to the homogenate, while that of alkaline phosphatase is not. On the other hand, the fraction I material from androgen-stimulated mice exhibits a further increase in specific activity with respect to ß-glucuronidase and not acid phosphatase. It thus appears that there is an independence in the behavior of individual enzymes in response to physiologic stimuli in spite of obvious morphologic proximity.

THE ROLE OF THE PITUITARY GLAND IN IMPLANTATION IN THE MOUSE: DELAY OF IMPLANTATION BY HYPOPHYSECTOMY AND NEURODEPRESSIVE DRUGS

1969 ◽  
Vol 43 (2) ◽  
pp. 225-235 ◽  
Author(s):  
B. M. BINDON
Keyword(s):  
Pituitary Gland ◽  
Early Pregnancy ◽  
Single Injection ◽  
Hormone Replacement ◽  
Oestrous Cycle ◽  
The Other ◽  
Optimum Conditions ◽  
Delayed Implantation ◽  
Long Acting

SUMMARY The optimum conditions for delay of implantation by hypophysectomy and neurodepressive agents are described. Hypophysectomy on day 1 without hormone replacement was followed by retarded development and subsequent degeneration of zygotes. Viability of blastocysts was maintained under these conditions by a single injection of a long-acting progestagen on day 1. Hypophysectomy at intervals beginning late on day 3 indicated that implantation is initiated by pituitary activity in the several hours around midnight of this day. In animals induced to ovulate and copulate by exogenous gonadotrophin injections, the corresponding time of pituitary activity was delayed by approximately 8 hr. This delay could not be explained solely on the basis of altered times of ovulation. It is evident that the events of early pregnancy do not follow the normal physiological pattern under these conditions, and caution should be exercised in utilizing such animals. Of five neurodepressive agents examined, only trifluoperazine effectively delayed implantation. The effect of this substance injected at various times on day 3 of pregnancy suggests that implantation in the mouse is initiated by neurally regulated pituitary activity between 16.00 and 24.00 hr. on this day. Comparison with the mechanism of ovulation indicates that ovulation and implantation are regulated by separate hypothalamic-pituitary events, one peculiar to the oestrous cycle, the other to early pregnancy.

Composition of uterine flushings from Large White and prolific Chinese Meishan gilts

1991 ◽  
Vol 3 (1) ◽  
pp. 51 ◽  
Author(s):  
FW Bazer ◽  
WW Thatcher ◽  
F Matinat-Botte ◽  
M Terqui ◽  
MC Lacroix ◽  
...  
Keyword(s):  
Acid Phosphatase ◽  
Early Pregnancy ◽  
Immunoglobulin A ◽  
Oestrous Cycle ◽  
Large White ◽  
Sodium Potassium ◽  
Uterine Lumen ◽  
Pregnancy Status ◽  
Aminopeptidase P ◽  
Uterine Secretions

This study examined differences in selected components of uterine secretions from Large White and prolific Chinese Meishan gilts during the oestrous cycle or early pregnancy. Total recoverable protein, uteroferrin (measured as acid phosphatase activity), acyl aminopeptidase, calcium, sodium, potassium, immunoglobulins A and G, glucose, fructose, oestradiol-17 beta, and prostaglandins F2 alpha (PGF2 alpha) and E2 (PGE2) in uterine flushings were measured. During the oestrous cycle, breed effects were detected only for total protein (P = 0.07), which tended to be higher for Large White gilts. However, for pregnant gilts, total recoverable glucose (P less than 0.05), fructose (P less than 0.05) sodium (P less than 0.05), immunoglobulin A (P less than 0.01), PGF (P less than 0.01), PGE (P less than 0.01), and acyl aminopeptidase (P less than 0.05) were greater in uterine flushings from Meishan gilts. Only uteroferrin was higher (P = 0.06) in uterine flushings from Large White gilts. Concentrations of prolactin were higher (P less than 0.05) in plasma from cyclic or pregnant Meishan gilts, but concentrations of total oestrogens and progesterone were not affected by pregnancy status or breed. These results suggest that Meishan conceptuses, individually or collectively, are more stimulatory to endometrial secretion and/or transport of the components of histotroph into the uterine lumen, or that the endometrium of Meishan gilts is more sensitive to conceptus signals responsible for the accumulation of histotroph in the uterine lumen.

The Influence of Short Ether Anesthesia on Some Liver Enzyme Activities in the Rat

1973 ◽  
Vol 51 (8) ◽  
pp. 604-607 ◽  
Author(s):  
E. Katona
Keyword(s):  
Alkaline Phosphatase ◽  
Acid Phosphatase ◽  
Malate Dehydrogenase ◽  
Enzyme Activities ◽  
Urate Oxidase ◽  
Arylsulfatase A ◽  
Ether Anesthesia ◽  
Arylsulfatase B ◽  
Thiamine Pyrophosphatase ◽  
Liver Homogenates

Rats were anesthetized by ether inhalation for 4–5 min and sacrificed 1–48 h after anesthesia. From their liver homogenates, the activities of nine enzymes were determined. Activities of urate oxidase and arylsulfatase-A did not change significantly but arylsulfatase-B was slightly decreased. Malate dehydrogenase, arylsulfatase-B, and thiamine pyrophosphatase reached their highest and "malic enzymes" their lowest activities at the same time, 5 h after anesthesia. Alkaline phosphatase first decreased, later increased. Acid phosphatase and glucose-6-phosphatase activities decreased following ether anesthesia. Thesechanges in the enzyme activities generally agree and partly explain previously reported effects of ether anesthesia observed in the serum.

scholarly journals LEUKEMIA EVOKED WITH 7, 8, 12-TRIMETHYLBENZ(A)ANTHRACENE IN RAT

1971 ◽  
Vol 134 (5) ◽  
pp. 1285-1297 ◽  
Author(s):  
Colin Bird ◽  
Charles Huggins
Keyword(s):  
Stem Cells ◽  
Alkaline Phosphatase ◽  
Acid Phosphatase ◽  
Lactate Dehydrogenase ◽  
Subsequent Development ◽  
Nodule Formation ◽  
Leukemic Stem Cells ◽  
Immature Rats ◽  
Enzyme Changes ◽  
Red Pulp

An early sign of erythroblastic leukemia in rat was nodule formation in the spleen. Hyperplastic foci of stem cells, indistinguishable histologically from leukemic stem cells, were found in the red pulp whereas the malpighian corpuscles were uninvolved. Anemia is a normal phenomenon in immature rats and the spleen of the prepubertal rat possesses considerable hemopoietic potential. Pulse-doses of 7, 8, 12-trimethylbenz(a)anthracene prevented the physiologic hematological development of maturing rats and was associated with subsequent development of leukemic stem cells in the red pulp of the spleen. Significant enzyme changes were observed in leukemic spleens. Compared with the spleens of normal littermates, the concentration of lactate dehydrogenase rose while that of malate dehydrogenase fell; the content of alkaline phosphatase rose whereas acid phosphatase fell. Increased alkaline phosphatase activity in leukemic spleen was attributed to nonleukemic foci of myelopoiesis.

Hyphal tip growth in Achlya: Enzyme activities in mycelium and medium

1979 ◽  
Vol 57 (20) ◽  
pp. 2145-2149 ◽  
Author(s):  
T. W. Hill ◽  
J. T. Mullins
Keyword(s):  
Alkaline Phosphatase ◽  
Culture Filtrate ◽  
Enzyme Activities ◽  
Tip Growth ◽  
Specific Activity ◽  
Fresh Weight ◽  
Exogenous Glucose ◽  
Achlya Ambisexualis ◽  
Specific Activities ◽  
Hyphal Tip

Achlya ambisexualis Raper is a coenocytic, hyphal water mold which exhibits apical vegetative growth when cultured on a denned liquid medium. Upon transfer to a medium containing only glucose, growth is reduced considerably as measured by changes in fresh weight, incorporation of exogenous glucose into hyphal walls, and secretion of cellulase. Of the enzymes tested, only cellulase and alkaline phosphatase exhibit higher specific activities in growing mycelia than in nongrowing mycelia; the specific activity of UDPG transferase remains about the same. All enzyme activities found in mycelial extracts also were present in the culture filtrate. The only enzymes showing much higher activities in the filtrate than in the mycelium were ATPase and cellulase. Possible roles for cellulase during growth are discussed.

scholarly journals Adaptation of the small intestine during pregnancy and lactation in the rat

1979 ◽  
Vol 184 (2) ◽  
pp. 245-251 ◽  
Author(s):  
K Burdett ◽  
C Reek
Keyword(s):  
Small Intestine ◽  
Lactate Dehydrogenase ◽  
Isocitrate Dehydrogenase ◽  
Enzyme Activities ◽  
Unit Area ◽  
Mucosal Epithelium ◽  
Pregnancy And Lactation ◽  
Enzyme Change ◽  
Specific Activities ◽  
Glucose 6 Phosphate Dehydrogenase

During pregnancy and lactation in the rat the small intestine in general and the mucosal epithelium in particular gain weight. The specific activities of sucrase, lactate dehydrogenase and succinate-tetrazolium reductase remain constant and those of glucose 6-phosphate dehydrogenase and isocitrate dehydrogenase increase. There is no evidence that the reported decrease in absorption per unit area or weight of mucosal epithelium during pregnancy and lactation is due to decreases in enzyme activities within the epithelium. The pattern of enzyme change shows that the response of the gut to the stimuli of pregnancy and lactation must be a complex one, possibly involving increases in the specific activities of some enzymes.

scholarly journals Assessment of ranges plasma indices in rainbow trout (Oncorhynchus mykiss) reared under conditions of intensive aquaculture

2011 ◽  
Vol 59 (6) ◽  
pp. 181-188 ◽  
Author(s):  
Radovan Kopp ◽  
Jan Mareš ◽  
Štěpán Lang ◽  
Tomáš Brabec ◽  
Andrea Ziková
Keyword(s):  
Alkaline Phosphatase ◽  
Rainbow Trout ◽  
Acid Phosphatase ◽  
Lactate Dehydrogenase ◽  
Oncorhynchus Mykiss ◽  
Total Protein ◽  
Aspartate Aminotransferase ◽  
Statistical Testing ◽  
Plasma Parameters ◽  
Normal Ranges

Plasma parameters in rainbow trout (Oncorhynchus mykiss) from three various trout farms in the Czech Republic were assessed using automated blood plasma analyser. Non-haemolysed serum from the heart of 48 healthy, randomly selected fish (standard length, mean ± SD = 247.3 ± 24.2 mm; body mass, mean ± SD = 262.18 ± 87.28 g) was analysed for the following plasma parameters: alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, acid phosphatase, lactate dehydrogenase, creatine kinase, total protein, cholinesterase, amylase, glucose, lactate, albumin, urea, cholesterol, triglycerides, lipase, Ca, Mg, P, Fe, Na, K and Cl. All data were analysed statistically such as normality assessment by means of Kolmogorov–Smirnov test and adequate statistical testing using various parametric and non-parametric tests for each variable. With regard to data distribution, 19 indices out of 23 (aspartate aminotransferase, alkaline phosphatase, acid phosphatase, lactate dehydrogenase, total protein, amylase, glucose, lactate, albumin, urea, cholesterol, triglycerides, Ca, Mg, P, Fe, Na, K and Cl) were normally distributed. The indices were affected by handling time and, accordingly to the physical and chemical properties of water. Estimates obtained were compared with previously reported ranges. The blood automated analyser proved to be a valuable and reliable instrument for the estimation of plasma parameters determining normal ranges in rainbow trout.

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