scholarly journals Stability of lactate dehydrogenase, aspartate aminotransferase, alkaline phosphatase and tartrate resistant acid phosphatase in human saliva and gingival crevicular fluid in the presence of protease inhibitor

2013 ◽  
Vol 65 (3) ◽  
pp. 1131-1140 ◽  
Author(s):  
Nurfathiha Kasim ◽  
Shahrul Ariffin ◽  
Muhammad Shahidan ◽  
Intan Abidin ◽  
Sahidan Senafi ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Acid Phosphatase ◽  
Lactate Dehydrogenase ◽  
Protease Inhibitor ◽  
Aspartate Aminotransferase ◽  
Gingival Crevicular Fluid ◽  
Human Saliva ◽  
Tartrate Resistant Acid Phosphatase ◽  
Crevicular Fluid

scholarly journals Assessment of ranges plasma indices in rainbow trout (Oncorhynchus mykiss) reared under conditions of intensive aquaculture

2011 ◽  
Vol 59 (6) ◽  
pp. 181-188 ◽  
Author(s):  
Radovan Kopp ◽  
Jan Mareš ◽  
Štěpán Lang ◽  
Tomáš Brabec ◽  
Andrea Ziková
Keyword(s):  
Alkaline Phosphatase ◽  
Rainbow Trout ◽  
Acid Phosphatase ◽  
Lactate Dehydrogenase ◽  
Oncorhynchus Mykiss ◽  
Total Protein ◽  
Aspartate Aminotransferase ◽  
Statistical Testing ◽  
Plasma Parameters ◽  
Normal Ranges

Plasma parameters in rainbow trout (Oncorhynchus mykiss) from three various trout farms in the Czech Republic were assessed using automated blood plasma analyser. Non-haemolysed serum from the heart of 48 healthy, randomly selected fish (standard length, mean ± SD = 247.3 ± 24.2 mm; body mass, mean ± SD = 262.18 ± 87.28 g) was analysed for the following plasma parameters: alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, acid phosphatase, lactate dehydrogenase, creatine kinase, total protein, cholinesterase, amylase, glucose, lactate, albumin, urea, cholesterol, triglycerides, lipase, Ca, Mg, P, Fe, Na, K and Cl. All data were analysed statistically such as normality assessment by means of Kolmogorov–Smirnov test and adequate statistical testing using various parametric and non-parametric tests for each variable. With regard to data distribution, 19 indices out of 23 (aspartate aminotransferase, alkaline phosphatase, acid phosphatase, lactate dehydrogenase, total protein, amylase, glucose, lactate, albumin, urea, cholesterol, triglycerides, Ca, Mg, P, Fe, Na, K and Cl) were normally distributed. The indices were affected by handling time and, accordingly to the physical and chemical properties of water. Estimates obtained were compared with previously reported ranges. The blood automated analyser proved to be a valuable and reliable instrument for the estimation of plasma parameters determining normal ranges in rainbow trout.

scholarly journals Effect of orthodontic forces on levels of enzymes in gingival crevicular fluid (GCF): A systematic review

2019 ◽  
Vol 24 (2) ◽  
pp. 40.e1-40.e22 ◽  
Author(s):  
Priyanka Kapoor ◽  
Nitika Monga ◽  
Om Prakash Kharbanda ◽  
Sunil Kapila ◽  
Ragini Miglani ◽  
...  
Keyword(s):  
Systematic Review ◽  
Acid Phosphatase ◽  
Tooth Movement ◽  
Gingival Crevicular Fluid ◽  
Orthodontic Tooth Movement ◽  
Tartrate Resistant Acid Phosphatase ◽  
Orthodontic Force ◽  
Hand Search ◽  
Force Application ◽  
Crevicular Fluid

Abstract Objective: Orthodontic force application releases multiple enzymes in gingival crevicular fluid (GCF) for activation, resorption, reversal, deposition of osseous elements and extracellular matrix degradation. The current systematic review critically evaluated all existing evidence on enzymes in orthodontic tooth movement. Methods: Literature was searched with predetermined search strategy on electronic databases (PubMed, Scopus, Embase), along with hand search. Results: Initial search identified 652 studies, shortlisted to 52 studies based on PRISMA. Quality assessment further led to final inclusion of 48 studies (13 moderately and 35 highly sensitive studies). Primary outcomes are significant upregulation in GCF levels of enzymes-aspartate aminotransferase (AST), alkaline phosphatase (ALP), matrix metalloproteinases (MMPs), lactate dehydrogenase (LDH), β-glucuronidase (βG), tartrate resistant acid phosphatase (TRAP), acid phosphatase (ACP) and down regulation in cathepsin B (Cb). Site specificity is shown by ALP, TRAP, AST, LDH, MMP9 with levels at compression site increasing earlier and in higher quantities compared with tension site. ALP levels are higher at tension site only in retention. A positive correlation of LDH, ALP and AST is also observed with increasing orthodontic force magnitude. Conclusions: A strong evidence of variation in enzymes (ALP, AST, ACP TRAP, LDH, MMPs, Cb) in GCF is found in association with different magnitude, stages and sites of orthodontic force application.

scholarly journals Bone-Derived Serum Enzymes and Bone Density in Perimenopausal Caucasian Women

1993 ◽  
Vol 30 (2) ◽  
pp. 191-194 ◽  
Author(s):  
J D Johnston ◽  
S Koneru ◽  
T Kuwana ◽  
S B Rosalki
Keyword(s):  
Alkaline Phosphatase ◽  
Acid Phosphatase ◽  
Bone Density ◽  
Femoral Neck ◽  
Serum Levels ◽  
Bone Alkaline Phosphatase ◽  
Serum Enzymes ◽  
Tartrate Resistant Acid Phosphatase ◽  
Vertebral Bone ◽  
Caucasian Women

Serum levels of bone-origin alkaline phosphatase and of tartrate-resistant acid phosphatase were measured in Caucasian women aged 41–69 years who had volunteered for bone densitometry. Bone alkaline phosphatase and tartrate-resistant acid phosphatase were inversely correlated with vertebral bone density and with femoral neck bone density. Bone alkaline phosphatase and acid phosphatase were also significantly correlated, consistent with the concept of ‘coupling’ between osteoblast and osteoclast activity.

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