OESTRONE, OESTRADIOL AND TESTOSTERONE IN NORMAL AND HYPOGONADAL MEN FOLLOWING LH-RH OR HCG STIMULATION

1976 ◽  
Vol 81 (3) ◽  
pp. 616-622 ◽  
Author(s):  
H. K. Kley ◽  
E. Nieschlag ◽  
W. Wiegelmann ◽  
H. L. Krüskemper
Keyword(s):  
Leydig Cell ◽  
Cell Function ◽  
Healthy Men ◽  
Pathological Conditions ◽  
Function Test ◽  
Parallel Pattern ◽  
Normal Men ◽  
Lh Rh ◽  
Secondary Hypogonadism ◽  
Leydig Cell Function

ABSTRACT Oestrone, oestradiol-17β and testosterone in serum were measured during a LH-RH infusion test and a Leydig cell function test with HCG in healthy men and in patients with primary or secondary hypogonadism. Following infusion of LH-RH increases of LH (+112%), oestradiol (+62 %) and testosterone (+51 %) were observed in normal men, while oestrone remained unchanged. Of the patients only those with Klinefelter's syndrome showed a significant increase in testosterone and oestradiol after LH-RH infusion. During the Leydig cell function test oestradiol and testosterone exhibited a largely parallel pattern under normal and pathological conditions as well as in subjects with experimental hypogonadism. Augmentations of plasma oestrone were usually smaller than those for oestradiol.

scholarly journals Evaluation of endocrine testing of Leydig cell function using extractive and recombinant human chorionic gonadotropin and different doses of recombinant human LH in normal men

2008 ◽  
Vol 159 (2) ◽  
pp. 171-178 ◽  
Author(s):  
Anne Cailleux-Bounacer ◽  
Yves Reznik ◽  
Bruno Cauliez ◽  
Jean François Menard ◽  
Céline Duparc ◽  
...  
Keyword(s):  
Chorionic Gonadotropin ◽  
Leydig Cell ◽  
Cell Function ◽  
Dose Effect ◽  
Plasma Testosterone ◽  
Testosterone Levels ◽  
Plasma Estradiol ◽  
Normal Men ◽  
Dose Dependent ◽  
Leydig Cell Function

BackgroundThe functional testing of endocrine testis uses extractive human chorionic gonadotropin (ehCG). Recombinant human hCG (rhCG), avoiding any contamination, should replace ehCG. Moreover, a functional evaluation with recombinant human LH (rhLH) would be closer to physiology than a pharmacological testing with hCG.MethodsThe study was conducted in normal men. We first evaluated the dose–effect of ehCG on plasma testosterone and estradiol levels, before and after injection of either hCG or vehicle. Secondly, the responses to the optimal dose of ehCG were compared with those of rhCG. Thirdly, we investigated the dose–effect of rhLH, on steroid hormone secretion. LH, testosterone, and estradiol plasma levels were measured after the injection of either rhLH or placebo.ResultsehCG induced dose-dependent increases in plasma estradiol and testosterone levels. They respectively peaked at 24 and 72 h after the injection. The most potent dose of ehCG (5000 IU) induced results similar to those observed with 250 μg (6500 IU) rhCG. By comparison with placebo, rhLH induced a significant and dose-dependent increase in plasma testosterone levels 4 h after the injection. Peak response of testosterone to rhLH and rhCG was significantly correlated. rhLH did not induce significant change in plasma estradiol level.ConclusionsIn normal men, a single i.v. injection of 150 IU rhLH induces a 25% rise in plasma testosterone levels by comparison with placebo. At the moment, the dynamic evaluation using hCG remains the gold standard test to explore the Leydig cell function. The use of 250 μg rhCG avoiding any contamination should be recommended.

LH, FSH AND TESTOSTERONE IN PLASMA FOLLOWING LH-RH INFUSION: A COMBINED TEST FOR PITUITARY AND LEYDIG CELL FUNCTION

1974 ◽  
Vol 75 (3) ◽  
pp. 417-427 ◽  
Author(s):  
H. K. Kley ◽  
W. Wiegelmann ◽  
E. Nieschlag ◽  
H. G. Solbach ◽  
H. Zimmermann ◽  
...  
Keyword(s):  
Cell Function ◽  
Plasma Concentrations ◽  
Normal Subjects ◽  
Significant Rise ◽  
Clinical Value ◽  
Combined Test ◽  
Normal Men ◽  
Lh Rh ◽  
Dose Dependent ◽  
Leydig Cell Function

ABSTRACT The effect of LH-RH (luteinizing hormone releasing hormone) on plasma concentrations of LH, FSH, testosterone (T) and cortisol was studied in 13 normal men and in 9 men with adrenal, testicular, pituitary and hypothalamic disorders. Rapid and dose-dependent (25–100 μg LH-RH) increases in plasma LH were observed and reached peak levels 20–30 min after the intravenous LH-RH injections. No rise in plasma T was seen with these doses. During an 8½ hour infusion of 300 μg LH-RH a rapid increase of LH occurred and the levels remained elevated for the duration of the infusion. The increase in FSH was neither as great nor as constant. Following stimulation of LH and FSH, a statistically significant rise in plasma T occurred in the normal subjects (20–70 %). No effect of LH-RH on the adrenal cortex could be detected by measuring cortisol during the infusion. In 3 patients with Addison's disease the increase in testosterone did not differ from that in normal subjects. From these studies a LH-RH infusion test for pituitary and testicular function was designed: 50 μg LH-RH iv at 8 a. m. followed by 250 μg infusion from 8:30 a. m. to 4:30 p. m. The clinical value of the test has been demonstrated in patients with Klinefelter's syndrome, craniopharyngioma and Kallmann's syndrome. The test provides a useful method for localizing the lesions in disorders of the hypothalamo-pituitary-testicular axis.

CHANGES IN LEYDIG CELL HYDROXYSTEROID DEHYDROGENASES INDUCED BY ADMINISTRATION OF TESTOSTERONE PROPIONATE TO NORMAL MEN

1974 ◽  
Vol 60 (1) ◽  
pp. 175-NP ◽  
Author(s):  
H. C. MORSE ◽  
C. G. HELLER
Keyword(s):  
Leydig Cell ◽  
Leydig Cells ◽  
Cell Function ◽  
Testosterone Propionate ◽  
Testicular Biopsy ◽  
Frozen Sections ◽  
Histochemical Technique ◽  
Biopsy Specimens ◽  
Normal Men ◽  
Leydig Cell Function

SUMMARY By the employment of an improved histochemical technique, frozen sections from human testicular biopsy specimens were examined for 3β-and 17β-hydroxysteroid dehydrogenase (HSD) activity, before, during, and after administration of 25 or 50 mg testosterone propionate per day to normal men. This administration strongly suppressed Leydig cell HSD activity and caused these cells to be transformed into fibroblast-like cells. After cessation of administration, the Leydig cells recovered morphologically and so did, simultaneously their 3β- and 17β-HSD activity. It is concluded that histochemically detectable 3β- and 17β-HSD are under gonadotrophin control and change with alterations in Leydig cell function. The morphology or 3β- and 17β-HSD are therefore probably acceptable as indicators of Leydig cell function in reproductively normal men.

DISORDERS OF LEYDIG CELL FUNCTION IN PRIMARY ADRENAL INSUFFICIENY

1974 ◽  
Vol 77 (1_Suppl) ◽  
pp. S61
Author(s):  
R. Mies ◽  
D. Heesen ◽  
W. Winkelmann
Keyword(s):  
Leydig Cell ◽  
Cell Function ◽  
Leydig Cell Function

FSH regulates cultured Leydig cell function via Sertoli cell proteins: An in vitro study

1985 ◽  
Vol 132 (2) ◽  
pp. 729-734 ◽  
Author(s):  
M. Benahmed ◽  
C. Grenot ◽  
E. Tabone ◽  
P. Sanchez ◽  
A.M. Morera
Keyword(s):  
Sertoli Cell ◽  
Leydig Cell ◽  
Cell Function ◽  
In Vitro Study ◽  
Vitro Study ◽  
Leydig Cell Function

Impaired Testicular Function in Patients With Carcinoma-In-Situ of the Testis

1999 ◽  
Vol 17 (1) ◽  
pp. 173-173 ◽  
Author(s):  
Peter Meidahl Petersen ◽  
Aleksander Giwercman ◽  
Steen W. Hansen ◽  
Jørgen G. Berthelsen ◽  
Gedske Daugaard ◽  
...  
Keyword(s):  
Testicular Cancer ◽  
Germ Cell ◽  
Leydig Cell ◽  
Carcinoma In Situ ◽  
Cell Function ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Testicular Dysfunction ◽  
Leydig Cell Function

PURPOSE: To elucidate the biologic association between germ cell neoplasia and testicular dysfunction, through investigation of Leydig cell function and semen quality in men with carcinoma-in-situ (CIS) of the testis. PATIENTS AND METHODS: We examined two groups of men, unilaterally orchidectomized for testicular cancer. Biopsy of the contralateral testis had showed CIS in a group of 24 patients and no evidence of CIS in the other group of 30 patients. Semen quality and serum levels of testosterone, luteinizing hormone (LH), and follicle-stimulating hormone (FSH) were compared in these two groups of men after orchidectomy but before further treatment for testicular cancer. RESULTS: Significantly higher LH levels (median, 8.1 IU/L v 4.8 IU/L; P < .001) and generally lower testosterone levels (median, 12.5 nmol/L v 15.5 nmol/L; P = .13) were found in the CIS group. The proportion of patients with Leydig cell dysfunction was higher in the group of patients with CIS (11 of 24) than in the group of patients without (two of 30) (P = .01). Sperm concentration and total sperm count were significantly lower (P < .001) in patients with CIS (median, 0.03 × 106/mL and 0.10 × 106, respectively) than in patients without (median, 9.1 × 106/mL and 32 × 106, respectively), whereas the levels of FSH were significantly higher (P < .001) in the former group of men (median, 19.6 IU/L v 9.0 IU/L). CONCLUSION: Not only spermatogenesis but also Leydig cell function is impaired in testes with CIS. This impairment could be due to common factors in the pathogenesis of germ cell neoplasm and testicular dysfunction. Alternatively, CIS cells may have a negative impact on Leydig cell function.

scholarly journals Leydig Cell Function in Long-term Testosterone-immunized Rats

1983 ◽  
Vol 4 (1) ◽  
pp. 95-103 ◽  
Author(s):  
RICHARD M. SHARPE ◽  
HAMISH M. FRASER
Keyword(s):  
Leydig Cell ◽  
Cell Function ◽  
Leydig Cell Function
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