AFFINITY AND SPECIFICITY OF ANTISERA AGAINST HUMAN FSH AND TSH OBTAINED BY IMMUNIZING RABBITS WITH HIGHLY PURIFIED HORMONE PREPARATIONS

1975 ◽  
Vol 78 (1) ◽  
pp. 22-31 ◽  
Author(s):  
P. A. Torjesen ◽  
T. Sand
Keyword(s):  
Pregnant Women ◽  
Association Constant ◽  
Association Constants ◽  
The Other ◽  
Specific Antiserum ◽  
Α Subunit ◽  
Specific Antisera ◽  
Scatchard Plots

ABSTRACT Two groups of 4 rabbits were immunized according to the method described by Vaitukaitis et al. (1971). One group was given 55 μg (900 IU) FSH/animal and the other 100 μg (0.5 IU) TSH/animal. Two highly specific antisera against FSH and one specific antiserum against TSH were obtained. The FSH antisera showed affinities towards LH and TSH of 0.13 and 0.25% respectively as compared to that of FSH. The TSH antisera showed affinities towards LH and FSH of 2.2 and 1.7 % respectively as compared to that of TSH. The specific antisera showed no or minor affinities towards the LH-α subunit and they were sufficient specific to be used in the radioimmunoassay of serum from pregnant women without pre-absorption with HCG. Scatchard plots showed the association constants of the FSH antisera to be 1.3 × 1011 and 1.2 × 1011 1/mol respectively, and the association constant of the TSH antiserum to be 6 × 1010 l/mol. All 3 antisera showed high titers.

scholarly journals Immunochemical specificity of the combining site of murine myeloma protein CAL20 TEPC1035 reactive with dextrans.

1981 ◽  
Vol 153 (1) ◽  
pp. 166-181 ◽  
Author(s):  
S Sugii ◽  
E A Kabat ◽  
M Shapiro ◽  
M Potter
Keyword(s):  
Binding Constant ◽  
Association Constant ◽  
Association Constants ◽  
The Other ◽  
Side Chains ◽  
Binding Properties ◽  
Myeloma Protein ◽  
Affinity Electrophoresis ◽  
Inhibition Data ◽  
Type Site

The immunochemical specificity of the combining sites of murine myeloma protein CAL20 TEPC1035 was studied by quantitative precipitin and precipitin inhibition assays. Myeloma protein CAL20 TEPC1035 precipitated with only three dextrans, B1355S4, B1498S, and B1501S, with high proportions of alpha(1 leads to 3) linkages, but not with any other dextrans, glycogen, and pullulan. Inhibition tests with various sugars show that the combining site of myeloma protein CAL20 TEPC1035 is most complementary to panose, a trisaccharide DGlc alpha(1 leads to 6)DGlc alpha(1 leads to 4)DGlc. Panose was 3.3 times more potent than a tetrasaccharide DGlc alpha(1 leads to 6)DGlc alpha(1 leads to 4)DGlc alpha(1 leads to 4)DGlc and 8, 23, 42, > 42 times more active than maltose, nigerose, isomaltose, and kojibiose, respectively. These findings were paralleled by their binding properties as determined by affinity electrophoresis. The association constants (Ka) of these three dextrans to myeloma protein CAL20 TEPC1035 ranged from 3.8 X 10(3) ml/g to 5.02 X 10(3) ml/g. The association constant of inhibitor (Kia) of panose was 8.19 X 10(3) M-1. Myeloma protein CAL20 TEPC1035 is an antidextran with specificity different from those of other murine myeloma antidextrans and from human antidextrans reported previously and its combining site size is at least as large as a trisaccharide. The binding constant of methyl alpha-D-glucoside (7.2 X 10(2)) was 73% of that of panose and comparable to that of myeloma protein W3129 (9.4 X 10(2)) with a cavity-type site and 600 times lower (1.6 X 10(0)) for QUPC52 with a groove type site, indicating that the terminal nonreducing residue is held in a cavity. Inhibition data with various alpha(1 leads to 4)-linked oligosaccharides also indicate that the internal portions of these inhibitors may react directly with a portion of the combining site. These findings suggest that myeloma antidextran CAL20 TEPC1035 has a partial cavity-type combining site in which the terminal nonreducing dGlc alpha(1 leads to 6) moiety is held in a cavity with the other two sugars forming a groove. However, oligosaccharides with one or more alternating [leads to 3DGlc alpha(1 leads to 6)DGlc alpha(1 leads to 3)DGlcl leads to] units with and without terminal nonreducing DGlc alpha(1 leads to 6) or DGLc alpha(1 leads to 3) side chains remain to be tested to determine whether structures known to be present in the three dextrans which precipitate CAL20 TEPC1035 may not prove to be more active than panose.

scholarly journals The binding of binuclear platinum(II)-terpyridine complexes to DNA

1987 ◽  
Vol 242 (1) ◽  
pp. 177-183 ◽  
Author(s):  
W D McFadyen ◽  
L P Wakelin ◽  
I A G Roos ◽  
B L Hillcoat
Keyword(s):  
Optical Absorption ◽  
Base Pair ◽  
Base Composition ◽  
Association Constant ◽  
Equilibrium Dialysis ◽  
Association Constants ◽  
Ligand Molecule ◽  
Aqueous Buffer ◽  
Scatchard Plots ◽  
Polyelectrolyte Theory

The binding of platinum (II)-terpyridine complexes to DNA was studied by using equilibrium dialysis. Optical absorption methods were used to measure the ability of the ligands to aggregate in aqueous buffer. Scatchard plots for the binding of the monomeric [Pt(terpy)SC4H9]+ cation to DNA at I0.01 are curvilinear, concave upwards, suggesting two modes of binding. The association constant decreases at higher ionic strengths, consistent with polyelectrolyte theory, and 1.1 cations are released per bound ligand molecule. The association constants of the binuclear ligands [Pt(terpy)S[CH2]4S(terpy)Pt]2+ and [Pt(terpy)S[CH2]6S(terpy)Pt]2+ are 8 and 23 times larger respectively than the affinity of the monomer. For the latter binuclear derivative the increase may be ascribed to bifunctional reaction. Differential dialysis experiments with DNAs of differing base composition show that [Pt(terpy)SC4H9]+ has a requirement for a single G X C base-pair at the highest-affinity site. However, in the binuclear ligands chromophore specificity is severely compromised. Similar experiments indicate that 9-aminoacridine and selected methylene-linked diacridines show no significant sequence selectivity.

Insulin binding to differentiating muscle cells in culture

1983 ◽  
Vol 61 (7) ◽  
pp. 644-649 ◽  
Author(s):  
Amira Klip ◽  
Grace Li ◽  
Denise Walker
Keyword(s):  
Binding Sites ◽  
Surface Membrane ◽  
Insulin Binding ◽  
Association Constants ◽  
The Other ◽  
Membrane Cholesterol ◽  
L6 Cells ◽  
Increasing Temperature ◽  
Scatchard Plots ◽  
Number Of Cells

Saturable binding of 125I-labelled insulin was detected on L6 myoblasts grown in monolayers. Binding was proportional to the number of cells and was pH sensitive, decreasing above pH 7.0. Binding also decreased with increasing temperature in the range 22–37 °C. Binding of insulin over a range of hormone concentrations (10−10–10−6 M) was analyzed by Scatchard plots. The data are compatible with the coexistence of two components with Kd of 3 and 190 nM. The number of both types of binding sites increased with cell differentiation (upon alignment and cell fusion), when expressed either per unit protein, DNA, or surface membrane cholesterol. On the other hand, their association constants did not vary. Binding of insulin to a nonfusing mutant of L6 cells resembled binding to the parent myoblasts before alignment.

Features of the course and treatment of glaucoma during pregnancy

2020 ◽  
Vol 98 (3) ◽  
pp. 178-184
Author(s):  
T. V. Chernyakova ◽  
A. Yu. Brezhnev ◽  
I. R. Gazizova ◽  
A. V. Kuroyedov ◽  
A. V. Seleznev
Keyword(s):  
Pregnant Women ◽  
Laser Treatment ◽  
Progressive Disease ◽  
Clinical Course ◽  
The Other ◽  
Third Trimester ◽  
The Third ◽  
Chronic Progressive Disease ◽  
Hypotensive Therapy ◽  
Eye Pressure

In the review we have integrated all up-to-date knowledge concerning clinical course and treatment of glaucoma among pregnant women to help specialists choose a proper policy of treatment for such a complicated group of patients. Glaucoma is a chronic progressive disease. It rarely occurs among childbearing aged women. Nevertheless the probability to manage pregnant patients having glaucoma has been recently increasing. The situation is complicated by the fact that there are no recommendations on how to treat glaucoma among pregnant women. As we know, eye pressure is progressively going down from the first to the third trimester, so we often have to correct hypotensive therapy. Besides, it is necessary to take into account the effect of applied medicines on mother health and evaluate possible teratogenic complications for a fetus. The only medicine against glaucoma which belongs to category B according to FDA classification is brimonidine. Medicines of the other groups should be prescribed with care. Laser treatment or surgery may also be a relevant decision when monitoring patients who are planning pregnancy or just bearing a child. Such treatment should be also accompanied by medicines.

Abnormal vascular function and morphology in preeclampsia: a study of isolated resistance vessels

1985 ◽  
Vol 69 (4) ◽  
pp. 477-482 ◽  
Author(s):  
C. Aalkjaer ◽  
H. Danielsen ◽  
P. Johannesen ◽  
E. B. Pedersen ◽  
A. Rasmussen ◽  
...  
Keyword(s):  
Angiotensin Ii ◽  
Pregnant Women ◽  
Vascular Function ◽  
Direct Evidence ◽  
The Other ◽  
Vascular Changes ◽  
Direct Information ◽  
Vascular Abnormalities ◽  
Media Thickness ◽  
Resistance Vessels

1. In order to obtain direct information about vascular changes associated with pre-eclampsia, the morphological and functional characteristics of isolated omental resistance vessels from 11 women with pre-eclampsia, 10 normotensive pregnant women and eight normotensive nonpregnant women were determined. 2. In vessels from the women with preeclampsia, the ratio of media thickness to lumen diameter was increased, compared with that in vessels from the other two groups. 3. The vessels from the women with preeclampsia had an increased responsiveness to angiotensin II and a decreased rate of relaxation, but only when compared with the vessels from the normotensive pregnant women. However, no difference in responsiveness to noradrenaline was found between any of the groups. 4. The angiotensin II responsiveness of the vessels from the women with pre-eclampsia and from the non-pregnant women were similar, suggesting that pre-eclampsia is associated with an absence of the change in vascular function which normally occurs during pregnancy. 5. The study provides direct evidence for an involvement of vascular abnormalities in the pathogenesis of pre-eclampsia.

scholarly journals KEHAMILAN TETAP SEHAT DI MASA PANDEMI

2021 ◽  
Vol 4 (3) ◽  
pp. 631
Author(s):  
Riona Sanjaya ◽  
Hellen Febriyanti ◽  
Yetty Dwi Fara ◽  
Septika Yani Veronica ◽  
Siti Maesaroh ◽  
...  
Keyword(s):  
Health Education ◽  
Pregnant Women ◽  
Growth And Development ◽  
Natural Process ◽  
Theory And Practice ◽  
The Other ◽  
Normal Length ◽  
Other Hand ◽  
Keywords Pregnancy

ABSTRAKKehamilan merupakan proses yang normal, alamiah yang diawali dengan pertumbuhan dan perkembangan janin intrauteri, dimulai sejak konsepsi sampai bersalin dan lamanya kehamilan normal adalah 280 hari (40 minggu atau 9 bulan 7 hari) di hitung dari hari pertama haid terakhir.Pada masa Kehamilan ibu hamil agar dapat mempersiapkan diri pada kesehatan ibu hamil dengan menjaga nutrisi selama proses kehamilannya. Ibu hamil wajib memeriksakan kehamilannya agara ibu hamil dapat mengetahui dan mencegah sedini mungkin kelainan yang dapat terjadi, meningkatkan kondisi badan ibu dalam menghadapi kehamilan serta mendapatkan penyuluhan yang diperlukan selama kehamilan. Melalui kegiatan ini diharapkan masyarakat khususnya ibu hamil mampu menjaga kesehatannya selama kehamilan di masa pandemi. Kegiatan-kegiatan ini mencakup teori dan praktek yang meliputi : (1) Pendidikan Kesehatan yang dilakukan secara online; (2) Praktik Senam Ibu Hamil yang dilakukan Melalui Vidio; dengan melibatkan mahasiswa Universitas Aisyah Pringsewu pada saat pelaksanaan. Melalui kegiatan ini, diharapkan masyarakat khususnya ibu hamil dapat menyadari pentingnya kesehatan bagi ibu hamil walaupun dimasa pandemi ini. Kata kunci: kehamilan; nutrisi; senam hamil ABSTRACTPregnancy is a normal, natural process that begins with the growth and development of the intrauterine fetus. It starts from conception to delivery and the normal length of pregnancy is 280 days (40 weeks or 9 months 7 days) on the first day of the last menstruation. During pregnancy, pregnant women can prepare themselves for the health of pregnant women by maintaining nutrition during the pregnancy process. Pregnant women are required to have their pregnancy checked so that pregnant women can find out and prevent possible abnormalities that can occur, improve the condition of the mother's body in facing pregnancy and get the necessary counseling during pregnancy. Through this activity, it is expected that the community, especially pregnant women, will be able to maintain their health during pregnancy during a pandemic. These activities include theory and practice as follows: (1) Health Education which will be conducted online; (2) Exercise for Pregnant Women through Video; by involving the students of Aisyah University of Pringsewu during the implementation. In the other hand, it can realize the importance of health for pregnant women even during this pandemic. Keywords: pregnancy; nutrition; pregnancy exercise

scholarly journals Immunoassay of human chorionic gonadotropin, its free subunits, and metabolites

1997 ◽  
Vol 43 (12) ◽  
pp. 2233-2243 ◽  
Author(s):  
Laurence A Cole
Keyword(s):  
Normal Pregnancy ◽  
The Other ◽  
Β Subunit ◽  
Α Subunit ◽  
Core Fragment ◽  
Different Types ◽  
The Stability ◽  
In Pregnancy ◽  
Serum And Urine ◽  
Serum And Urine Samples

Abstract Multiple hCG-related molecules are present in pregnancy serum and urine samples. These include nonnicked hCG (the hormone), nicked hCG, hyper- and hypoglycosylated hCG, hCG missing the C-terminal extension, free α-subunit, large free α-subunit, free β-subunit, nicked free β-subunit, and β-core fragment. Over 100 immunoassays are sold for quantifying hCG-related molecules in serum or urine. Each measures nonnicked hCG and one of seven combinations of the other hCG-related molecules. This is the source of interassay discordance in hCG determinations. Whereas minor variations are noted in different kit results in normal pregnancy samples (more than twofold variation), much larger variations may be found in two immunoassay results in irregular gestations (spontaneous abortion, aneuploidy, preeclampsia, cancers, and trophoblast disease). Care is needed in choosing an immunoassay. What the assay measures may be more important than its cost or speed. This article reviews the structure of hCG and related molecules. It examines the stability and degradation of hCG, and recognition of hCG-related molecules by different types of immunoassay. Also reviewed are new assays for specifically detecting these other hCG-related molecules.

Abnormalities of peripheral venous tone in women with pregnancy-induced hypertension

1986 ◽  
Vol 70 (2) ◽  
pp. 155-157 ◽  
Author(s):  
Karen Stainer ◽  
Rachel Morrison ◽  
C. Pickles ◽  
A. J. Cowley
Keyword(s):  
Blood Pressure ◽  
Pregnant Women ◽  
Inverse Correlation ◽  
The Other ◽  
Normal Blood ◽  
Pregnancy Induced Hypertension ◽  
Normal Blood Pressure ◽  
Venous Tone ◽  
Arterial Blood ◽  
Induced Hypertension

1. Forearm venous tone was measured in two groups of pregnant women: one group with pregnancy-induced hypertension and the other group with normal blood pressure. 2. The women with pregnancy-induced hypertension were venoconstricted in the forearm (P < 0.01) compared with the pregnant women with normal blood pressure. However, there was no difference in venous tone between the women with pregnancy-induced hypertension and nonpregnant women. 3. There was an inverse correlation between mean arterial blood pressure and forearm venous tone (r = −0.581, P < 0.001) for all the pregnant women studied. Further evaluation of peripheral venous tone may provide valuable information about the pathophysiology and treatment of women with pregnancy-induced hypertension.

Water Proton Relaxation Rate Enhancements and Association Constants for Mn(II) to Serum Proteins Determined by NMR T1 Measurements

2005 ◽  
Vol 60 (9-10) ◽  
pp. 807-812 ◽  
Author(s):  
Hatice Budak
Keyword(s):  
Serum Albumin ◽  
Human Serum ◽  
Relaxation Rate ◽  
Association Constant ◽  
Serum Proteins ◽  
Association Constants ◽  
Water Proton ◽  
Proton Relaxation ◽  
Rate Enhancement ◽  
Human Serum Proteins

Abstract The water proton relaxation rate enhancement of Mn(II) bound to bovine serum albumin (BSA) and the association constant for manganese to BSA have already been determined, but such determinations have not been done for human serum albumin (HSA) and other human serum proteins and also for human serum. In this work, NMR T1 values in aqueous solutions of serum proteins and serum were measured versus increasing concentration of Mn(II). Proton relaxation rate enhancements (ε*) caused by different manganese concentrations were determined for each solution and 1/ε* was fitted against concentrations of Mn(II). Proton relaxation rate enhancements (εb) of Mn(II) bound to albumin, γ-globulin, (α+β)- globulins and serum were found to be 13.69, 3.09, 8.62, and 10.87, respectively. Free and bound manganese fractions, resulted from each addition of Mn(II) to the sample, were determined by using corresponding (ε*) and the εb values. Association constants for Mn(II) to HSA and γ-globulin were calculated as 1.84 x 104 ᴍ-1 and 2.35 x 104 ᴍ-1, respectively. Present data suggest that the proton relaxation rate enhancement of Mn(II) in serum is caused by Mn(II) bound to various serum constituents. Data also suggest that association constants for Mn(II) to γ-globulin are nearly the same as that to HSA.

scholarly journals Complexation of 2,6-helic[6]arene and its derivatives with 1,1′-dimethyl-4,4′-bipyridinium salts and protonated 4,4'-bipyridinium salts: an acid–base controllable complexation

2019 ◽  
Vol 15 ◽  
pp. 1795-1804 ◽  
Author(s):  
Jing Li ◽  
Qiang Shi ◽  
Ying Han ◽  
Chuan-Feng Chen
Keyword(s):  
Association Constants ◽  
The Other ◽  
Acid Base ◽  
Polar Solvents ◽  
X Ray ◽  
H Nmr ◽  
Hydrogen Bonding Interactions ◽  
Acids And Bases ◽  
Multiple Hydrogen Bonding ◽  
Nmr Titrations

2,6-Helic[6]arene and its derivatives were synthesized, and their complexation with 1,1′-dimethyl-4,4′-bipyridinium and protonated 4,4'-bipyridinium salts were investigated in detail. It was found that the helic[6]arene and its derivatives could all form 1:1 complexes with both 1,1′-dimethyl-4,4'-bipyridinium salts and protonated 4,4'-bipyridinium salts in solution and in the solid state. Especially, the helic[6]arene and its derivatives containing 2-hydroxyethoxy or 2-methoxyethoxy groups exhibited stronger complexation with the guests than the other helic[6]arene derivatives for the additional multiple hydrogen bonding interactions between the hosts and the guests, which were evidenced by 1H NMR titrations, X-ray crystal structures and DFT calculations. Moreover, it was also found that the association constants (K a) of the complexes could be significantly enhanced with larger counteranions of the guests and in less polar solvents. Furthermore, the switchable complexation between the helic[6]arene and protonated 4,4'-bipyridinium salt could be efficiently controlled by acids and bases.

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