THE EFFECT OF EFFERENT DUCT LIGATION ON THE UPTAKE OF 65Zn BY THE EPIDIDYMIS AND VAS DEFERENS OF RHESUS MONKEYS (MACACA MULATTA)

1974 ◽  
Vol 77 (1) ◽  
pp. 186-192 ◽  
Author(s):  
S. K. Roy ◽  
B. S. Setty ◽  
Harish Chandra ◽  
Amiya B. Kar
Keyword(s):  
Macaca Mulatta ◽  
Rhesus Monkeys ◽  
Vas Deferens ◽  
Maximum Accumulation ◽  
Efferent Duct ◽  
Efferent Ducts ◽  
Zn Uptake ◽  
Duct Ligation ◽  
Different Parts

ABSTRACT The uptake of 65Zn by different segments of the epididymis (caput, proximal corpus, distal corpus and cauda) and vas deferens of rhesus monkeys was investigated. The vas showed maximum accumulation as compared to different parts of the epididymis. Ligation of the efferent ducts of the testis for 90 days resulted in the absence of sperms with a corresponding decrease in 65Zn uptake in the epididymis and vas deferens. The latter, however, continued to show a relatively higher uptake than the epididymis. The significance of these findings is discussed.

Effect of efferent duct ligation on the function of the blood-testis barrier in rats

Reproduction ◽  
2000 ◽  
pp. 13-18 ◽  
Author(s):  
L Tao ◽  
JL Zupp ◽  
BP Setchell
Keyword(s):  
Barrier Function ◽  
Mass Gain ◽  
Interstitial Tissue ◽  
Fluid Accumulation ◽  
Efferent Duct ◽  
Efferent Ducts ◽  
Interstitial Volume ◽  
Duct Ligation ◽  
Testis Mass ◽  
Blood Testis Barrier

The function of the blood-testis barrier has been assessed from the ratio of the Cr-EDTA space in the parenchyma to the measured interstitial volume in the testes of rats at various times after unilateral ligation of the efferent ducts. The barrier remained effective during the phase of fluid accumulation and testicular mass gain, which was linear for at least 24 h, but the testis mass began to decrease between 32 and 40 h after efferent duct ligation, and the Cr-EDTA space at 40 and 48 h after efferent duct ligation exceeded the volume of the interstitial tissue. This finding indicated that, at these times, the barrier to Cr-EDTA, which is normally excluded from the tubules, had broken down and the marker was entering the tubules. Thereafter, the Cr-EDTA space decreased again to be less than the interstitial tissue volume, indicating a restoration of the barrier function, although degeneration of the seminiferous epithelium continued to become more obvious. The present study is the first report of a reversible breakdown of the barrier, but the relevance of the breakdown to the effects on spermatogenesis requires further study.

A comparison of the effects of bilateral efferent duct ligation and of partial epididymectomy on the testes of rats

1990 ◽  
Vol 2 (4) ◽  
pp. 321 ◽  
Author(s):  
C Soler ◽  
C Blazquez ◽  
J Pertusa ◽  
M Nunez ◽  
J Nunez ◽  
...  
Keyword(s):  
Body Weight ◽  
Cross Sections ◽  
Seminiferous Tubules ◽  
Testis Weight ◽  
Efferent Duct ◽  
Efferent Ducts ◽  
Linear Decrease ◽  
Endocrine Effect ◽  
Duct Ligation ◽  
Exponential Decrease

Testis weight as a percentage of body weight did not change following bilateral ligation of the efferent ducts (EDL) close to the epididymis, whereas following removal of part of the epididymis between the site of ligation and a point close to the junction between the caput and corpus (PCE), testis weight first rose linearly until Day 4 and then showed an exponential decrease between Days 4 and 28. After EDL, the perimeter of the seminiferous tubules rose for the first 7 days and then remained elevated, whereas after PCE, there was a linear decrease between Days 4 and 28. Following EDL, the percentage of altered and degenerated tubular cross-sections rose to about 30% and 10%, respectively, during the first 7 days after operation and then remained constant; after PCE, the percentage of altered tubules reached a maximum of 54% by 4 days and then fell, whereas the percentage of degenerated tubules continued to rise to 95% by 28 days. It would appear that all the effects of removal of a portion of the epididymis cannot be explained by blockage of the excurrent ducts, and a specific endocrine effect of the epididymis on the testis is proposed.

scholarly journals Limitations Imposed by Testicular Blood Flow on the Function of Leydig Cells in Rats in vivo

1983 ◽  
Vol 36 (3) ◽  
pp. 285 ◽  
Author(s):  
BP Setchell ◽  
KAA Galil
Keyword(s):  
Blood Flow ◽  
Leydig Cells ◽  
Venous Blood ◽  
Testis Weight ◽  
Single Exposure ◽  
Efferent Duct ◽  
Efferent Ducts ◽  
Duct Ligation ◽  
Testicular Blood Flow

Testis blood flow per testis closely follows testis weight in rats made aspermatogenic by a single exposure of the testis to 43�C for 30 min or 500 rad (5 Gy) of irradiation from a caesium source, or following ligation of the efferent ducts. Aspermatogenesis following these treatments was associated with only minor changes in the concentrations of testosterone in peripheral blood before stimulation with human chorionic gonadotrophin (hCG), and a reduced responsiveness to hCG when testis weight had fallen after heating. The concentrations of testosterone in testicular venous blood was normal or above normal during aspermatogenesis resulting from heat or irradiation, and only slightly reduced following efferent duct ligation.

Localization of Immune Complexes in the Basement Membrane of the Ductuli Efferentes of the Rhesus Monkey

1980 ◽  
Vol 38 ◽  
pp. 456-457
Author(s):  
D. Marsh
Keyword(s):  
Basement Membrane ◽  
Fluorescence Microscopy ◽  
Rhesus Monkey ◽  
Immune Complex ◽  
Immune Complexes ◽  
Vas Deferens ◽  
Frozen Sections ◽  
Efferent Ducts ◽  
Complex Deposition ◽  
Sperm Antibodies

As a result of vasectomy, spermatozoa are confined to the epididymis and vas deferens, where they degenerate, releasing antigens that enter the circulation or are engulfed by macrophages. Multiple antigens of the sperm can elicit production of autoantibodies; circulating anti-sperm antibodies are found in a large percentage of vasectomized men, indicating the immunogenicity of the sperm. The increased prevalence of macrophages in the liomen of the rhesus monkey testicular efferent ducts after vasectomy led to further study of this region. Frozen sections were used for evaluation of immunopathological status by fluorescence microscopy with fluorescein-conjugated antibody. Subsequent granular deposits of immune complexes were revealed by positive immunofluorescence staining for complement. The immune complex deposition in the basement membrane surrounding the efferent ducts implies that this region is involved in antigen leakage (Fig. 1).

Comparison of prospective and concurrent metamemory judgments in Rhesus monkeys (Macaca mulatta)

2013 ◽  
Author(s):  
Emily Kathryn Brown ◽  
Victoria L. Templer ◽  
Robert R. Hampton
Keyword(s):  
Macaca Mulatta ◽  
Rhesus Monkeys
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