RED CELL ZINC AND RED CELL ZINC METALLOENZYMES IN HYPERTHYROIDISM

1974 ◽  
Vol 76 (3) ◽  
pp. 645-650 ◽  
Author(s):  
J. A. Pangaro ◽  
M. Weinstein ◽  
M. C. Devetak ◽  
R. J. Soto
Keyword(s):  
Carbonic Anhydrase ◽  
Alcohol Dehydrogenase ◽  
Lactic Dehydrogenase ◽  
Control Group ◽  
Red Cell ◽  
Malic Dehydrogenase ◽  
Carbonic Anhydrase B ◽  
Zinc Metalloenzymes ◽  
The Mean ◽  
Hyperthyroid Patients

ABSTRACT The activity of the following zinc metalloenzymes was measured in the erythrocytes of hyperthyroid patients: malic dehydrogenase (MDH), aldolase (ALD), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), alcohol dehydrogenase (ADH), and lactic dehydrogenase (LDH). A significant increase in the activities of ADH (0.8 ± 0.3 μmoles per min per g of haemoglobin) and GAPDH (30.5 ± 13.4) was found; the control values being 0.5 ± 0.3 and 7.6 ± 4.6 respectively. The activities of ALD, MDH, and of LDH were not significantly different from those of the control group. The mean red cell zinc in the hyperthyroid group was 31.6 ± 6.2 μg per g of haemoglobin in contrast to 50.4 ± 8.1 in the control group. It is concluded that none of the zinc metalloenzymes measured accounts for the diminution of erythrocyte zinc occurring in hyperthyroidism, a phenomenon that should be attributed to the inhibition of carbonic anhydrase B as reported previously.

Red-cell 2,3-diphosphoglycerate in patients with hyperthyroidism

1980 ◽  
Vol 93 (4) ◽  
pp. 424-429 ◽  
Author(s):  
J. L. Alvarez-Sala ◽  
M. A. Urbán ◽  
J. J. Sicilia ◽  
A. J. Diaz Fdez ◽  
F. Fdez Mendieta ◽  
...  
Keyword(s):  
Thyroid Hormones ◽  
Control Group ◽  
Red Cell ◽  
Oxygen Dissociation Curve ◽  
Dissociation Curve ◽  
Oxygen Dissociation ◽  
The Right ◽  
Serum Thyroid Hormones ◽  
Hyperthyroid Patients ◽  
2,3 Dpg

Abstract. In 21 hyperthyroid female patients studied on 29 occasions, high levels of red-cell 2,3-diphosphoglycerate (2,3-DPG) have been found (5.75 ± 0.7 mm) which, compared to a euthyroid control group (4.88 ± 0.4 mm), could not be accounted for by differences in haematocrit, haemoglobin or phosphataemia. A significant correlation was found (P < 0.05) between serum thyroid hormones and the 2,3-DPG concentration in the hyperthyroid patients. Eight of these patients were reexamined after treatment and normalization of thyroid function, showed a regression to normal 2,3-DPG values (4.81 ± 0.6 mm) which could not be attributed to variations in haematocrit, haemoglobin or phosphataemia either. We therefore deduce that the shift to the right in the haemoglobin oxygen dissociation curve observed in patients of this type may be due to an increase in the red-cell 2,3-DPG content.

Changes in serum enzyme levels after exercise in trained and untrained subjects

1962 ◽  
Vol 17 (6) ◽  
pp. 943-946 ◽  
Author(s):  
William M. Fowler ◽  
Sudhir R. Chowdhury ◽  
Carl M. Pearson ◽  
Gerald Gardner ◽  
Robert Bratton
Keyword(s):  
Lactic Dehydrogenase ◽  
Serum Levels ◽  
Work Output ◽  
Serum Enzyme ◽  
Malic Dehydrogenase ◽  
The Mean

Twenty-eight healthy trained and untrained men and eleven untrained women were exercised at several grades on a treadmill or in various athletic events. The serum levels of five enzymes (two transaminases, aldolase, and two dehydrogenases) were compared prior to and at intervals after work. Generally there was a rise after exercise which reached its peak 5 min after completion of the work. The greatest increment of enzyme increase was observed in the untrained subjects and the least in the trained athletes. As the total amount of work output increased the mean serum enzyme levels were correspondingly raised. Aldolase and lactic dehydrogenase showed the least change, malic dehydrogenase increased moderately, while the two transaminases manifested very significant rises after all forms of exercise. Submitted on September 5, 1961

scholarly journals Neutrophil to Lymphocyte Ratio, Platelet to Lymphocyte Ratio, Mean Platelet Volume and Red Cell Distribution Width Measures in Bells Palsy

2017 ◽  
Vol 5 (1) ◽  
pp. 14-18 ◽  
Author(s):  
Caner Sahin ◽  
Ceyhun Varım
Keyword(s):  
Absolute Neutrophil Count ◽  
Neutrophil Count ◽  
Control Group ◽  
Red Cell ◽  
Cell Distribution ◽  
Platelet Volume ◽  
Platelet To Lymphocyte Ratio ◽  
Distribution Width ◽  
Lymphocyte Ratio ◽  
The Mean

AIM: The purpose of this study was to investigate the usefulness of the neutrophil to lymphocyte ratio (NLR), platelet to lymphocyte ratio (PLR) Mean Platelet Volume (MPV) and Red Cell Distribution Width (RDW) in the differential diagnosis and follow-up of patients with Bells Palsy.MATERIAL AND METHODS: Twenty-eight patients diagnosed with Bells Palsy and 28 control patients were included in the study. Serum samples were analysed retrospectively on the initial presentation and the seventh day of admission.RESULTS: On admission, the NLR was 1.7±1.2. The mean absolute neutrophil count was 6100 ± 900/mm^3 in Bells Palsy Group. NLR was 0.9 ± 0.2. The mean absolute neutrophil count was 4400 ± 1100/mm^3 in control group. Statistically, significant changes were not observed in NLR, PLR, MPV and RDW measurements in Bells Palsy group between House-Brackman Staging.CONCLUSION: Statistically significant changes in the neutrophil count and NLR were determined in the measurements between Bells Palsy and control group (p = 0.013, p = 0.016 respectively) on admission. A grade of the disease and NLR measurements had no statistically significant connection. RDW value was investigated for the first time in the literature for Bells Palsy patients.

scholarly journals Investigation of neutrophil-to-lymphocyte ratio and red cell distribution width in sudden senseurineural hearing loss

2019 ◽  
Vol 6 (2) ◽  
pp. 424
Author(s):  
Caner Sahin ◽  
Ceyhun Varıim ◽  
Cengiz Karacaer
Keyword(s):  
Hearing Loss ◽  
Sensorineural Hearing Loss ◽  
Sudden Sensorineural Hearing Loss ◽  
Control Group ◽  
Red Cell ◽  
Cell Distribution ◽  
Distribution Width ◽  
Lymphocyte Ratio ◽  
The Mean ◽  
And Control

Background: Sudden hearing loss (DHL) is a sudden sensorineural hearing loss effecting et least 3 or more frequencies more than 30 Decibel. The purpose of this study was to investigate the usefulness of the neutrophil to lymphocyte ratio (NLR) and Red Cell Distribution Width (RDW) in the first diagnosis of sudden sensorineural hearing loss (SSHL).Methods: Total 24 patients diagnosed with SSHL and 24 control patients included in the study. Serum samples were analyzed retrospectively on the initial presentation.Results: On admission, the NLR was 2.1±1.1 The mean absolute neutrophil count was 7100±400/mm3, and the mean complete lymphocyte count was 3400±1100/mm3. RDW was 11.9±0.6. Eight patients had total healing, 12 patients had partial cure, and two patients had no healing in the study. Statistically significant changes in NLR determined in the measurements between SSHL and control group (p<0.05). Significant differences were not observed in hemoglobin and hematocrit values, lymphocyte counts, RDW measurements between SSHL and control group (p <0.05).Conclusions: In lights of this information we recommend to screen NLR levels in SSHL patients. This may help us follow up patients recovery and if the patients recover from the disease higher level of NLR may create doubt for recurrence of the disease in risky patients.

Vitamin E and Neonatal Hemolysis

PEDIATRICS ◽  
1977 ◽  
Vol 59 (6) ◽  
pp. 995-997
Author(s):  
Steven J. Gross ◽  
Stephen A. Landaw ◽  
Frank A. Oski
Keyword(s):  
Vitamin E ◽  
Premature Infant ◽  
Total Dose ◽  
Treated Group ◽  
Mean Value ◽  
Control Group ◽  
Red Cell ◽  
Vitamin E Deficiency ◽  
Cell Life ◽  
The Mean

A study was designed to determine if the presence of vitamin E deficiency during the first week of life played a contributory role in the shortened red cell life span observed in the premature infant. Carboxyhemoglobin values were used as an index of hemolysis. Ten infants received vitamin E administered intramuscularly in a total dose of 125 mg/kg during days 3 to 7 of life; ten infants served as controls. The mean percent carboxyhemoglobin level fell significantly from day 3 to day 8 in the treated group (1.08% to 0.78%) whereas the mean value remained unchanged at 0.96% in the control group. The administration of vitamin E appears to reduce but not eliminate the accelerated red cell destruction that characterizes the preterm infant.

Hagfish (Myxine glutinosa) red cell membrane exhibits no bicarbonate permeability as detected by (18)O exchange

2000 ◽  
Vol 203 (10) ◽  
pp. 1551-1560 ◽  
Author(s):  
T. Peters ◽  
R.E. Forster ◽  
G. Gros
Keyword(s):  
Carbonic Anhydrase ◽  
Cell Membrane ◽  
Blood Cells ◽  
Carbonic Anhydrase Activity ◽  
Red Cell ◽  
Red Cell Membrane ◽  
Myxine Glutinosa ◽  
Uncatalyzed Reaction ◽  
Mass Spectrometric Technique ◽  
The Mean

The bicarbonate permeability of the plasma membrane of intact hagfish (Myxine glutinosa) red blood cells and the intracellular carbonic anhydrase activity of these cells were determined by applying the (18)O exchange reaction using a special mass spectrometric technique. When the macromolecular carbonic anhydrase inhibitor Prontosil-Dextran was used to suppress any extracellular carbonic anhydrase activity, the mean intracellular acceleration of the CO(2) hydration/HCO(3)(−) dehydration reaction over the uncatalyzed reaction (referred to as intracellular carbonic anhydrase activity A(i)) was 21 320+/−3000 at 10 degrees C (mean +/− s.d., N=9). The mean bicarbonate permeability of the red blood cell membrane (P(HCO3)-) was indistinguishable from zero. It can be concluded that CO(2) transport within hagfish blood does not follow the classical scheme of CO(2) transport in vertebrate blood. It is suggested that the combination of considerable intraerythrocytic carbonic anhydrase activity and low P(HCO3)- may serve to enhance O(2) delivery to the tissue in the exceptionally hypoxia-tolerant hagfish.

HUMAN LYMPHOCYTE BINDING AND DEIODINATION OF THYROID HORMONES IN RELATION TO THYROID FUNCTION

1975 ◽  
Vol 80 (4) ◽  
pp. 642-656 ◽  
Author(s):  
Ann-Charlotte Holm ◽  
Thérèse Lemarchand-Béraud ◽  
Bianca-Rosa Scazziga ◽  
Serge Cuttelod
Keyword(s):  
Thyroid Hormones ◽  
Thyroid Function ◽  
Human Lymphocyte ◽  
High Serum ◽  
Affinity Constant ◽  
Control Group ◽  
Binding Characteristics ◽  
The Mean ◽  
Hypothyroid Patients ◽  
Hyperthyroid Patients

ABSTRACT The human lymphocyte has been investigated regarding its function as a thyroid hormone target cell. Binding and deiodination of the thyroid hormones were determined after simultaneous incubation of 131I-labelled L-thyroxine (131I-T4) and 125I-labelled L-triiodothyronine (125I-T3) with lymphocytes from healthy subjects, from hyperthyroid and primary hypothyroid patients before and after treatment. The mean percentages of binding, 8.0 ± 0.5 (mean ± sem) for 131I-T4, and 9.7 ± 0.4 for 125I-T3 in the control group, were increased in the hyperthyroids to 10.1 ± 0.4 and 12.7 ± 0.6 respectively, and in the hypothyroids to 10.9 ± 0.7 and 12.8 ± 0.6. All elevated values returned to normal with successful treatment. The mean percentage of deiodination, 12.0 ± 1.7 for 131I-T4, and 6.5 ± 0.9 for 125I-T3 in the control group, showed a threefold increase in the hyperthyroid patients, to 35.9 ± 3.2 and 20.2 ± 1.9 respectively and remained unaltered in the hypothyroid patients. The values of successfully treated hyperthyroid patients were normal and those of the treated hypothyroid patients below normal. Human TSH added to the incubation medium stimulated the binding of both hormones, without influencing deiodination. Thus TSH might be active at the peripheral cellular level. This could contribute to the explanation of the increased binding by lymphocytes from primary hypothyroid patients with high serum concentrations of TSH. A preliminary analysis of the binding characteristics revealed an equilibrium affinity constant of 1.03·1010 m−1 for T3, and of 3.97· 109 m−1 for T4, with corresponding total numbers of binding sites of 1500 and 2000 per cell. It is concluded that, since lymphocyte activities closely reflect the thyroid function, these cells are well suited for studies on the peripheral fate of thyroid hormones and their cellular receptors.

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