STUDIES IN CONGENITAL GENERALIZED LIPODYSTROPHY

1973 ◽  
Vol 73 (4) ◽  
pp. 731-739 ◽  
Author(s):  
Oddmund Sövik ◽  
Svein Oseid ◽  
Stephanie Öyasæter
Keyword(s):  
Glucose Tolerance ◽  
Glucose Tolerance Test ◽  
Tolerance Test ◽  
Immunoreactive Insulin ◽  
List Type ◽  
Glucose Load ◽  
Crystalline Insulin ◽  
Congenital Generalized Lipodystrophy ◽  
Small Peak ◽  
Two Peaks

ABSTRACT Plasma from 3 subjects with congenital generalized lipodystrophy was filtered on Sephadex G-50, and the fractions obtained were analyzed for immunoreactive insulin (IRI). In one patient (I.T.) plasma was obtained in the course of an iv glucose tolerance test, during which there was a substantial increase of IRI. In the fasting state IRI was recovered as a single peak with an elution volume corresponding to crystalline insulin. Twenty min after the glucose load the insulin immunoreactivity appeared in two peaks, a major peak corresponding to crystalline insulin ("little" insulin) and a small peak corresponding to beef pro-insulin ("big" insulin). The relative amount of "big" insulin remained small during the glucose tolerance test. Twenty min after the glucose load the amount of "big" insulin was 4 per cent, whereas 6 per cent was found after 170 min. In a second patient (L.S.N.) with a substantially, lower IRI-activity "big" insulin was not detected after a glucagon load. Likewise, in a third patient (A.E.) with frank diabetes, studied after the age of puberty, a tolbutamide tolerance test failed to reveal any significant amount of "big" insulin.

STUDIES IN CONGENITAL GENERALIZED LIPODYSTROPHY

1973 ◽  
Vol 72 (3) ◽  
pp. 495-505 ◽  
Author(s):  
Oddmund Søvik ◽  
Svein Oseid
Keyword(s):  
Biological Activity ◽  
Insulin Response ◽  
Epididymal Adipose Tissue ◽  
Large Individual ◽  
Immunoreactive Insulin ◽  
List Type ◽  
Glucose Load ◽  
Congenital Generalized Lipodystrophy ◽  
The One

ABSTRACT The biological activity of plasma insulin from 4 cases of congenital generalized lipodystrophy has been studied, using rat diaphragm and epididymal adipose tissue in vivo. The results are compared with previous data on plasma immunoreactive insulin obtained in these patients. 2 of the 4 cases exhibited unusually high biological insulin activities during the fasting state as well as after an intravenous (iv) glucose load. In the fat pad assay activities as high as 10 000 μU insulin per ml were observed. During childhood the biological insulin activities were generally high, although there were large individual variations. However, in the one case studied after the age of puberty, the insulin response to a glucose load was negligible. Taken together, the biological and immunological activities observed strongly suggest the presence of pancreatic insulin in these patients. It appears that the circulating insulin has a fully biological activity. The decreasing insulin activities after cessation of growth are in agreement with the appearance of frank diabetes at this time.

Evidence that the oral glucose-tolerance test does not provide a uniform stimulus to pancreatic islets in pregnancy.

1989 ◽  
Vol 35 (7) ◽  
pp. 1482-1485 ◽  
Author(s):  
E A de Leacy ◽  
D M Cowley
Keyword(s):  
Glucose Tolerance ◽  
Oral Glucose Tolerance Test ◽  
Pancreatic Islets ◽  
Glucose Tolerance Test ◽  
Insulin Response ◽  
Tolerance Test ◽  
Glucose Absorption ◽  
Oral Glucose ◽  
Oral Glucose Tolerance ◽  
Glucose Load

Abstract Fifty consecutive pregnant patients referred for a glucose-tolerance test were classified on the basis of increasing (n = 20) or decreasing (n = 28) hematocrit after an oral 75-g glucose load. (The hematocrit did not change in the other two patients.) Patients with increasing hematocrit, a response previously seen in patients with the dumping syndrome, showed significantly flatter increases in glucose concentrations in plasma after the load. The mean decrease in the concentration of phosphate in plasma, measured as an index of glucose uptake by cells, was significantly less (P less than 0.05) 2 h after the load in the group with flatter glucose responses, suggesting that the flat response is ascribable to poor glucose absorption rather than to an exaggerated insulin response. These results indicate that the oral glucose-tolerance test stresses the pancreatic islets differently in different pregnant subjects, owing to individual variations in the gastrointestinal handling of the glucose load. Consequently, patients may give a "normal" result who might otherwise become hyperglycemic after normal meals. We suggest that alternative screening procedures be investigated to assess pregnant patients postprandially.

scholarly journals Glycemic Effects of Honey Compared to Glucose Using Standard OGTT

2017 ◽  
Vol 7 (2) ◽  
pp. 95-100
Author(s):  
Lubna Naznin ◽  
Muhammad Rabiul Hossain ◽  
Debashish Saha ◽  
Sarmin Sultana ◽  
Mreenal Kanti Sarkar
Keyword(s):  
Glucose Tolerance ◽  
Glucose Level ◽  
Plasma Glucose ◽  
Plasma Glucose Level ◽  
Glucose Tolerance Test ◽  
Tolerance Test ◽  
Oral Glucose ◽  
Oral Glucose Tolerance ◽  
Glucose Load ◽  
Load State

Background: Honey, though rich in fructose and glucose, had been shown to have plasma glucose lowering effect. It may be as a result of insulin sensitization, enhanced insulin secretion and anti-oxidant activity.Objective: This study was designed to assess the glycemic effects of honey comparing to glucose.Materials and Methods: The study was carried out at Armed Forces Institute of Pathology (AFIP), Dhaka cantonment from September, 2015 to October, 2015 on 35 individuals who reported to AFIP for ‘Oral Glucose Tolerance Test (OGTT)’. They were categorized to three groups based on OGTT ? Normal, Impaired glucose homeostasis (IGT or IFG), and Diabetes mellitus. On the subsequent day they were subjected to 52 mL honey load (equivalent to 75 gm by weight) to assess plasma glucose level after 1 hour and 2 hours posthoney load state. Student t-test was done to compare between means of plasma glucose level 1 hour after 75 gm glucose load and 1 hour after 75 gm honey load and also between means of plasma glucose level 2 hours after 75 gm glucose load and 2 hours after 75 gm honey load in the same individuals.Results: In all the three groups mean plasma glucose level in post-honey load state was found declined compared to post-glucose load state in both 1 hour and 2 hours specimens of HTT (Honey Tolerance Test) versus OGTT (Oral Glucose Tolerance Test) and this reduction was statistically significant (p<0.05).Conclusion: The study findings provide evidence that honey consumption causes less change in plasma glucose level than the equivalent quantity of oral glucose load regardless of status of glucose homeostasis. Further well designed researches are needed to determine the long term effects and beneficial quantity of honey, particularly in relation to diabetes mellitus.J Enam Med Col 2017; 7(2): 95-100

Short-Term Effect of a 75 g Oral Glucose Load on Glycohaemoglobin Levels

1987 ◽  
Vol 24 (1) ◽  
pp. 53-57 ◽  
Author(s):  
Richard D Forrest ◽  
Caroline A Jackson ◽  
Barry J Gould ◽  
Marianne Casburn-Budd ◽  
Julie E Taylor ◽  
...  
Keyword(s):  
Schiff Base ◽  
Glucose Tolerance ◽  
Glucose Tolerance Test ◽  
Tolerance Test ◽  
Assay Method ◽  
Oral Glucose ◽  
Oral Glucose Load ◽  
Glucose Load ◽  
Agar Gel ◽  
Electrophoretic Method

Two hundred and twenty-three subjects out of a total of 347 with various degrees of glucose tolerance were recalled after a screening survey for diabetes. They were a randomly selected sample of people over the age of 40 and they underwent a formal 75 g glucose tolerance test in order to assess the effect of a glucose load on glycohaemoglobin levels measured by four different assay methods. Oral glucose loading was found to affect glycohaemoglobin levels only when these were measured by an agar-gel electrophoretic method that did not remove the labile aldimine-linked Schiff base fraction. The increase in glycohaemoglobin during the glucose tolerance test as estimated by this method was proportional to the 2 h blood glucose level. Glycohaemoglobin levels measured by agar-gel electrophoresis with elimination of the Schiff base, by affinity chromatography and by iso-electric focussing, were not affected by a 75 g oral glucose load. We conclude that blood samples for glycohaemoglobin assay may be collected at any time of the day, without regard to the subject's previous food intake, provided an assay method is used that removed the aldimine-linked labile fraction.

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