DISTRIBUTION OF 14C-THIOURACIL IN THE THYROID AND THE ULTIMOBRANCHIAL GLAND OF CHICKENS STUDIED BY WHOLE-BODY AUTORADIOGRAPHY

1972 ◽  
Vol 71 (2) ◽  
pp. 265-272 ◽  
Author(s):  
Premysl Slanina
Keyword(s):  
Whole Body ◽  
High Accumulation ◽  
Whole Body Autoradiography ◽  
Ultimobranchial Gland

ABSTRACT Whole-body autoradiography of chicks 10, 20, 240 minutes and 1 and 4 days atfer iv injection of 14C-thiouracil showed a rapid and high accumulation of radioactivity in the thyroid. The concentration of radioactivity in the thyroid was higher than in any other tissue and persisted for as long as 4 days after the injection. In contrast, the level of radioactivity in the calcitonin producing ultimobranchial gland never exceeded that of the blood, suggesting that thiouracil does not directly affect the release of calcitonin.

STUDIES ON THE TISSUE-DISPOSITION AND FATE OF [14C]STREPTOZOTOCIN WITH SPECIAL REFERENCE TO THE PANCREATIC ISLETS

1978 ◽  
Vol 89 (2) ◽  
pp. 339-351 ◽  
Author(s):  
Eva Britt Johansson ◽  
Hans Tjälve
Keyword(s):  
Pancreatic Islets ◽  
High Capacity ◽  
Whole Body ◽  
Side Chain ◽  
High Accumulation ◽  
Methyl Group ◽  
Whole Body Autoradiography ◽  
Selective Labelling ◽  
Chinese Hamsters ◽  
Tissue Disposition

ABSTRACT The tissue-disposition and fate of [14C]streptozotocin, labelled in the methyl-group of the N-nitrosomethylurea side-chain, have been studied in mice. Whole-body autoradiography, quantified by densitometric measurements, showed that the pancreatic islets had a high capacity to accumulate radioactivity after the injection of [14C]streptozotocin. Microautoradiography of the pancreas showed that centrally located cells were labelled while peripherally located cells contained a low labelling, indicating a selective labelling of the β-cells. A high radioactivity was present in the liver and the cortex of the kidney at most survival intervals. About 17 % of the radioactivity was exhaled as 14CO2 during 6 h, which shows that the methyl group of the N-nitrosomethylurea side-chain is split off. Radioactivity was shown to be incorporated in the acid-insoluble precipitate of the pancreatic islets, the liver, the kidney, and the exocrine pancreas. This may, to a varying extent, be due both to alkylating reactions and to incorporation of radioactivity in the macromolecules of the tissues via normal metabolic pathways. About 44 % of the radioactivity was excreted as unchanged [14C]streptozotocin in the urine during 24 h, while about 1 % of the radioactivity was found in the faeces. Whole-body autoradiography of [14C]streptozotocin in two Chinese hamsters and one rat also showed a high accumulation of radioactivity in the pancreatic islets in these species.

ACCUMULATION OF NICOTINE IN PANCREATIC ISLETS AND CALCITONIN-PRODUCING CELLS IN MICE AND CHICKS DEMONSTRATED BY MICRO- AND WHOLE-BODY AUTORADIOGRAPHY

1973 ◽  
Vol 58 (1) ◽  
pp. 21-NP ◽  
Author(s):  
PREMYSL SLANINA ◽  
HANS TJÄLVE
Keyword(s):  
Pancreatic Islets ◽  
Biogenic Amines ◽  
Whole Body ◽  
High Accumulation ◽  
Metabolic Studies ◽  
Whole Body Autoradiography ◽  
Parafollicular Cells ◽  
Ultimobranchial Glands ◽  
Endocrine Organs

SUMMARY By autoradiographic methods, nicotine was shown to be specifically accumulated in the pancreatic islets in mice. The results also indicated a high accumulation of nicotine in the parafollicular cells of the thyroid in mice and an accumulation was also shown in the ultimobranchial glands in chicks. Like the parafollicular cells of the thyroid in mammals, the ultimobranchial glands of birds are known to produce calcitonin. Metabolic studies with nicotine in vitro and autoradiographic studies with the main nicotine-metabolite cotinine, indicated an accumulation of unchanged nicotine (not metabolites) in the cells. The results are discussed in view of the fact that biogenic amines have been shown to be operative in these endocrine organs. It is suggested that nicotine can share common transport and/or storage mechanisms with biogenic amines in the cells. An effect of nicotine on hormone storage and/or release may take place via an interference with aminergic mechanisms in the cells.

scholarly journals WHOLE-BODY AUTORADIOGRAPHY OF DISTRIBUTION OF [1-14C]-2-DEOXYGLUCOSE IN MICE

1980 ◽  
Vol 13 (2) ◽  
pp. 202-210 ◽  
Author(s):  
MASAHITO WATANABE ◽  
TAKASHI KIHARA ◽  
MASAHISA SHIMADA ◽  
KIYOHISA KURIMOTO
Keyword(s):  
Whole Body ◽  
Whole Body Autoradiography

Whole body autoradiography and microautoradiography in eels after intra-arterial administration of 125I-labeled eel ANP

1996 ◽  
Vol 271 (4) ◽  
pp. R926-R935 ◽  
Author(s):  
H. Sakaguchi ◽  
H. Suzuki ◽  
H. Hagiwara ◽  
H. Kaiya ◽  
Y. Takei ◽  
...  
Keyword(s):  
Binding Sites ◽  
Head Kidney ◽  
Dorsal Aorta ◽  
Whole Body ◽  
Whole Body Autoradiography ◽  
Target Organs ◽  
Secondary Lamellae ◽  
Ventral Aorta ◽  
Interrenal Cells ◽  
Kidney Liver

125I-labeled eel atrial natriuretic peptide (ANP) was administered into the ventral or dorsal aorta of freshwater (FW) and seawater (SW) eels, Anguilla japonica, and the major target organs were explored by whole body autoradiography. Localization of the ANP binding in the target organs was also examined at tissue and cell levels by microautoradiography using tissue sections. Whole body autoradiography revealed that the specific label was accumulated predominantly in the gill, with lesser amounts in the atrium, kidney, liver, and urinary bladder. Autoradiographic grains were most dense in the secondary lamellae of the gill, particularly on the side of the efferent filamental artery. Other binding sites in target tissues were the glomerulus of the kidney, epicardium and endocardium of the atrium, bile duct/blood vessels of the liver, and interrenal cells of the head kidney. There was no difference in the distribution and density of grains between injections into the ventral aorta and dorsal aorta, although, in the former, injected 125I-labeled eel ANP passes through the gill before reaching peripheral target tissues. There was a tendency for downregulation of ANP binding sites in SW eels, especially in the gill. These results show that specific ANP binding sites are present in organs that are implicated in osmoregulation and cardiovascular regulation in eels and further suggest that the number of ANP binding sites varies according to changes in the environmental salinity.

Whole Body Autoradiography

2004 ◽  
Author(s):  
Kenneth Brouwer ◽  
Lee Crossman ◽  
Jeanne Jarrett
Keyword(s):  
Whole Body ◽  
Whole Body Autoradiography

Distribution of 14C-labelled Atrazine, Methoxychlor, Glyphosate, and Bisphenol-A in Goldfish Studied by Whole-Body Autoradiography (WBARG)

2008 ◽  
Vol 43 (4) ◽  
pp. 265-274 ◽  
Author(s):  
Claude Rouleau ◽  
Jagmohan Kohli
Keyword(s):  
Bisphenol A ◽  
Carassius Auratus ◽  
Environmental Risks ◽  
Whole Body ◽  
Intestinal Lumen ◽  
Whole Body Autoradiography ◽  
Goldfish Carassius Auratus ◽  
Uveal Tract ◽  
Target Organs ◽  
Freeze Dried

Abstract Nonpersistent contaminants represent thousands of chemicals used as pesticides, pharmaceuticals, personal care products, additives, etc. Because of this diversity, the assessment of the environmental risks they may pose for the environment represents a formidable task. Identification of target organs is key information needed to orient further research on newlyinvestigated organic xenobiotics. We used whole-body autoradiography to visualize the distribution of 14C-labelled atrazine, methoxychlor, glyphosate, and bisphenol-A in goldfish (Carassius auratus) and identify target organs. Fish were exposed for 2 days (glyphosate and bisphenol-A) and 7 days (atrazine and methoxychlor) to the radiolabelled compounds at a concentration of 15 nM. They were then frozen, embedded in carboxymethylcellulose gel, 20-μm-thick cryosections were collected, freeze-dried, and exposed to phosphor screens to visualize the tissue distribution of radioactivity. Goldfish did not accumulate glyphosate. The three other compounds were accumulated, mostly in the gall bladder. Nevertheless, unforeseen accumulation sites were observed; atrazine accumulated in the uveal tract of the eye, high levels of radioactivity were found in the cerebrospinal fluid of goldfish exposed to methoxychlor, and an important accumulation of bisphenol-A was seen in urine, oral mucosa, esophagus, and intestinal lumen. The potential toxicological consequences of the accumulation of these chemicals at very specific locations within the fish body are discussed and further research suggested.

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