EFFECTS OF LITHIUM-CHLORIDE ON THE ISLETS OF LANGERHANS IN GUINEA-PIGS

1972 ◽  
Vol 70 (4) ◽  
pp. 731-735 ◽  
Author(s):  
Birger Petersson ◽  
Baron Shopsin
Keyword(s):  
B Cells ◽  
Islets Of Langerhans ◽  
Pancreatic Islet ◽  
Lithium Chloride ◽  
Guinea Pigs ◽  
Islet Cells ◽  
Nuclear Size ◽  
Pancreatic Islet Cells

ABSTRACT Daily lithium-chloride injections were given for about one month to guinea-pigs. Caryometric analyses of the nuclear size in the pancreatic islet cells revealed a marked increase in the nuclear size in the A1-cells, while there were no changes in the A2- and B-cells.

Monolayer cultures of pancreatic islet cells from adult rats: effect of 2-deoxyglucose

1988 ◽  
Vol 118 (2) ◽  
pp. 173-NP ◽  
Author(s):  
M. Aoki ◽  
S. Kagawa ◽  
T. Yamamura ◽  
A. Matsuoka ◽  
J. Utsunomiya
Keyword(s):  
Insulin Secretion ◽  
B Cells ◽  
Pancreatic Islet ◽  
Cell Function ◽  
Islet Cells ◽  
Pancreatic Tissue ◽  
Pancreatic Islet Cells ◽  
Adult Rats ◽  
Monolayer Cultures

ABSTRACT Techniques for the monolayer culture of pancreatic islet cells from adult rats and the responsiveness of B cells are described. Whole pancreatic tissue was enzymatically dispersed and then cultured for 30 days in tissue culture medium 199 containing 5·5 mmol glucose/l, with or without 1 mmol 2-deoxyglucose/l. In the absence of 2-deoxyglucose, the responsiveness of B cells diminished to almost zero by day 15 and islets degenerated. In contrast, addition of 2-deoxyglucose to the medium resulted in a selective degeneration of fibroblasts, yielding monolayers that consisted mostly of islet cells. In this stationary system in which monolayers of islet cells were maintained in medium with 2-deoxyglucose, insulin secretion from B cells on days 15 and 30 increased in a dose-dependent fashion in response to increasing concentrations of glucose, leucine and 2-ketoisocaproate. Similarly, when exposed to 16·7 mmol glucose/l, perifused B cells showed a biphasic pattern of insulin secretion on day 15. Addition of 10 μmol forskolin/l and 200 nmol 12-O-tetradecanoyl phorbol13-acetate/l remarkably enhanced this response. Likewise, the response to 10 mmol leucine/l or 10 mmol 2-ketoisocaproate/l was biphasic. These results suggest that these monolayer cultures retain the functional properties of the adult rat pancreas, and may be useful not only as a model for the in-vitro study of B cell function, but also for implantation. J. Endocr. (1988) 118, 173–178

Monolayer cultures of adult pancreatic islet cells on osmotically disrupted fibroblasts

Diabetes ◽  
1980 ◽  
Vol 29 (6) ◽  
pp. 497-500 ◽  
Author(s):  
P. Meda ◽  
E. L. Hooghe-Peters ◽  
L. Orci
Keyword(s):  
Pancreatic Islet ◽  
Islet Cells ◽  
Pancreatic Islet Cells ◽  
Monolayer Cultures

Use of liposomes to introduce substances into pancreatic islet cells

Diabetes ◽  
1988 ◽  
Vol 37 (8) ◽  
pp. 1123-1128
Author(s):  
N. Welsh ◽  
A. Hallberg ◽  
S. Sandler ◽  
C. Hellerstrom
Keyword(s):  
Pancreatic Islet ◽  
Islet Cells ◽  
Pancreatic Islet Cells

Superfusion of dissociated pancreatic islet cells attached to Cytodex beads

Diabetes ◽  
1982 ◽  
Vol 31 (3) ◽  
pp. 189-193 ◽  
Author(s):  
Y. Spiess ◽  
M. A. Smith ◽  
W. Vale
Keyword(s):  
Pancreatic Islet ◽  
Islet Cells ◽  
Pancreatic Islet Cells
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