PLASMA ALDOSTERONE DETERMINATION WITH 35S-p-TOLUENE-SULPHONIC ANHYDRIDE

1972 ◽  
Vol 70 (3) ◽  
pp. 552-566 ◽  
Author(s):  
D. Scholer ◽  
A. M. Riondel ◽  
E. L. Manning
Keyword(s):  
Plasma Level ◽  
Thin Layer ◽  
Water Samples ◽  
Regression Line ◽  
Mean Value ◽  
Plasma Aldosterone ◽  
Peripheral Plasma ◽  
Derivative Method ◽  
Replicate Measurements ◽  
Double Isotope

ABSTRACT The double isotope derivative method for plasma aldosterone, (Bojesen & Thuneberg 1967), has been applied to human peripheral plasma. 35S-p-toluene-sulphonic anhydride was used as the labelled reagent (100–150 mCi/mEq.) and 3H-aldosterone (30 Ci/mm; 0.05 ng added to each sample) as indicator. The initial derivative (aldosterone-21-35S-tosylester) was transformed to its 11,18-γ-lactone and finally to its 3-dinitrophenylhydrazone. Purification was achieved by 1 paper chromatography and 4 thin-layer chromatographies. The overall-recovery was 10 ± 2.7 (sd)%, n = 134. The non-specific blank was 0.053 ± 0.044 (sd), when determined on water samples, and 0.068 ± 0.034 (sd) ng/5 ml, when determined on adrenalectomised human plasmas (non-specific water blank of the same series: 0.064±0.037 (sd) ng). The addition of a known amount of aldosterone to a plasma resulted in a linear relationship over the range examined: y = (0.947 ± 0.013) × + 0.696, P = 0.000, and aldosterone from 1.0 to 5.0 ng was measured with an accuracy of 95%. Replicate measurements of different volumes of the same plasma from 1.0 to 7.5 ml showed a calculated regression line of y = (0.148 ± 0.005) × −0.01, P = 0.002. Replicate measurements of the same plasma (n = 8) gave a mean value of 0.14±0.01 (sd) ng, a range between 0.13 and 0.15 ng and a coefficient of variation of 7%. Thus, it was possible to determine a peripheral plasma level of 7 ng/100 ml in 2 ml plasma and a level of 3.5 ng/100 ml in 4 ml plasma.

A Comparison of Double-Isotope Derivative and Radioimmunological Estimation of Plasma Aldosterone Concentration in Man

1973 ◽  
Vol 45 (3) ◽  
pp. 411-415 ◽  
Author(s):  
R. Fraser ◽  
Sheena Guest ◽  
Jessie Young
Keyword(s):  
Large Scale ◽  
Plasma Aldosterone ◽  
Plasma Aldosterone Concentration ◽  
Plasma Pool ◽  
Wide Range ◽  
Significant Difference ◽  
Derivative Method ◽  
Scale Population ◽  
Suitable Technique ◽  
Double Isotope

1. Two techniques for estimating plasma aldosterone concentration are compared by means of repeated assays of a plasma pool and also by analysis of a wide range of plasma samples. 2. No significant difference was found in the results obtained by the methods. Radioimmunoassay required only one tenth of the volume of plasma needed for the double-isotope derivative method. 3. Its rapidity and relative inexpensiveness makes radioimmunoassay at present the most suitable technique for large-scale population screening.

A DOUBLE ISOTOPE DERIVATIVE METHOD FOR THE DETERMINATION OF ALDOSTERONE IN PERIPHERAL PLASMA

1961 ◽  
Vol 37 (4) ◽  
pp. 541-558 ◽  
Author(s):  
Ejgil Bojesen ◽  
Hans Degn
Keyword(s):  
Isotope Dilution ◽  
Half Life ◽  
Acid Anhydride ◽  
Hydroxyl Group ◽  
Aldosterone Level ◽  
Peripheral Plasma ◽  
Derivative Method ◽  
Double Isotope

ABSTRACT The details of a method for the determination of aldosterone at the level of 1 ng or more are described. A combination of the isotope derivative principle and the isotope dilution principle, using 35S and 131I labelled piodobenzenesulfonic acid anhydride (pipsan) as the labelled reagents, has been employed. A quantitative or almost quantitative and reproducible esterification of the 21-hydroxyl group of aldosterone and an 80% recovery of the steroid added to plasma can be achieved by the procedure described. The blank value has been determined by the analysis of epripheral plasma of adrenalectomized dogs and was found to be indistinguishable from zero and therefore less than 3 ng per 100 ml of plasma. In salt depleted anaesthetized dogs the aldosterone level was 20–30 ng per 100 ml. The concentration increased considerably during adrenalectomy and decreased with a »half life« of 20–30 minutes from the time this was completed.

PROCEDURE FOR DETERMINATION OF ALDOSTERONE IN HUMAN PERIPHERAL PLASMA BY DOUBLE-ISOTOPE DERIVATIVE ASSAY, AND ITS APPLICATION FOR MEASUREMENT OF SECRETORY RATE AND URINARY EXCRETION

1967 ◽  
Vol 45 (12) ◽  
pp. 1919-1936 ◽  
Author(s):  
Wojciech Nowaczynski ◽  
Jack Silah ◽  
Jacques Genest
Keyword(s):  
Acetic Anhydride ◽  
Urinary Excretion ◽  
Specific Activity ◽  
High Specific Activity ◽  
Plasma Aldosterone ◽  
Chromatographic Purification ◽  
Secretory Rate ◽  
Peripheral Plasma ◽  
Double Isotope

This is a report of a procedure for the determination of peripheral plasma aldosterone, in which 14C-labeled aldosterone serves as the marker, and the acetylating agent is relatively inexpensive tritium-labeled acetic anhydride of high specific activity. A new method for the chromatographic purification of aldosterone and aldosterone diacetate is presented. This procedure provides a better separation of these two compounds from the unlabeled and labeled impurities.

scholarly journals Stable Carbon and Sulfur Isotope Characteristics of Stream Water in a Typical Karst Small Catchment, Southwest China

Water ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 523
Author(s):  
Yang Tang ◽  
Ruiyin Han
Keyword(s):  
Water Samples ◽  
Chemical Weathering ◽  
Stream Water ◽  
Mean Value ◽  
Small Catchment ◽  
Temperature And Precipitation ◽  
Δ13c Value ◽  
Different Seasons ◽  
Sources Of Solutes ◽  
Surface Water Samples

Surface water samples from the Maolan National Natural Reserved Park (MNNRP) were analyzed from Sept. 2013 to June 2014, for major ion concentrations (K+, Na+, Ca2+, Mg2+, Cl−, SO42−, HCO3−), δ13C-DIC and δ34S-SO42− to quantify the sources of solutes and chemical weathering. The results show that HCO3− and SO42− are the main anions in Banzhai watershed, which account for 86.2 and 10.4% of the total anion equivalent, respectively. While Ca2+ and Mg2+ account for 76.9 and 20.5%, respectively. Considerable Mg2+ in stream water indicates that it may be affected by dolomite weathering. stream water samples present the δ13C-DIC values in the range of −16.9‰~−10.8‰ (mean value was −13.9‰), which were lower than that of the groundwater. The δ34S-SO42− values ranged from −15.2‰ to 1.7‰ (mean value was −4.4‰). There was a negative correlation between HCO3− content and δ13C value, implying the result of the interaction of temperature and precipitation intensity in different seasons. The significant positive correlation between SO42− content and δ13C-DIC indicates that H2SO4 may be involved in the weathering process of carbonate rocks in small watershed scale. The content of SO42− in a school sample site was much higher than that of other sample sites for the interference from human sources. The δ34S values show that the average δ34S-SO42− in most sites is close to the δ34S isotopic values of Guizhou coal and rain, indicating that they may be affected by local coal.

Stable isotope geochemistry of Oxygen and Hydrogen: A case study of the Satluj River Basin, India  

2021 ◽  
Author(s):  
Akhtar Jahan ◽  
Mohd Usman Khan ◽  
Nachiketa Rai ◽  
Abhayanand Singh Maurya ◽  
Sudhir Kumar
Keyword(s):  
Stable Isotope ◽  
River Water ◽  
Water Samples ◽  
Hydrological Cycle ◽  
Indian Subcontinent ◽  
Monsoon Season ◽  
Isotope Analysis ◽  
Mean Value ◽  
Isotopic Analyses ◽  
The Mean

<p>Stable isotope analysis of hydrogen and oxygen is one of the important methods used to model the hydrological cycle. Oxygen and hydrogen isotopic investigation of river water, its tributaries, and groundwater of its catchment from the Satluj basin was undertaken to estimate the contributions of the main sources comprising discharge during major periods throughout a hydrologic year.</p><p>Estimation of the snow/glaciers melt contribution is also very important for tracing the sources and processes regulating the flow from the provenance and reservoirs in the context of global warming, for estimating flood flow, and for other water resource development activities in large parts of the Indian subcontinent. Water samples were collected during the non-monsoon season at increasing altitudes. In this work, in addition to stable isotopes, we also assessed the water quality using various physicochemical parameters and geochemistry of the water.</p><p>From isotopic analyses of river water samples, the mean value of the δ<sup>18</sup>O was found to be ~ -13‰, and the mean value of δD was found to be~ -85‰. For the samples from Satluj tributaries, the mean value of the δ<sup>18</sup>O was ~ -11‰, and the mean value of δD was ~ -69‰. A mean value of -8.4‰, was found based on the δ<sup>18</sup>O measurements of the groundwater samples, while the average δD value was found to be ~ -55‰.</p><p>For the mainstream and tributary, LWL, y = 8.2604x +20.208, and range of d-excess (>10‰) and y = 8.2079x + 22.182 and d-excess > 10‰ indicates a system recharged by sources of recycled moisture derived from continental sources in addition to monsoonal climates. For the groundwater data, the slope is 6.7, and d-excess ranges from 7‰ to 17‰. These observations are suggestive of the monsoonal source of Indian Ocean precipitation that has experienced significant evaporation during the non-monsoon season.</p><p>Our new data clearly shows that the surface water whether mainstream, tributary, and groundwater isotopes are homogenized from regional trends in precipitation, modified by evaporation, and are thus greatly influenced by latitude, elevation, and patterns of climate.</p><p> </p>

Quantitation of Plasma Aldosterone by Radioimmunoassay

1972 ◽  
Vol 18 (11) ◽  
pp. 1395-1402 ◽  
Author(s):  
M J St Cyr ◽  
J M Sancho ◽  
J C Melby
Keyword(s):  
Thin Layer ◽  
Upright Position ◽  
Plasma Aldosterone ◽  
Chromatographic System ◽  
Normal Plasma ◽  
Accuracy And Precision

Abstract Aldosterone antibodies were produced by injecting sheep with the albumin conjugate of aldosterone-18,21-dihemisuccinate. Conditions optimal for an accurate, reproducible assay with this antiserum were determined experimentally. Aldosterone is extracted from plasma and isolated by a single thin-layer chromatographic system before the immunoassay is performed. Normal plasma aldosterone in supine subjects is 8.6 ± 3.8 ng/100 ml; after 2 h in the upright position, 18.3 ± 9.5 ng/100 ml. Eighteen months of experience with the method have proved it to be reliable with respect to reproducibility, accuracy, and precision.

ULTRADIAN AND CIRCADIAN RHYTHMS IN THE PLASMA CONCENTRATION OF CORTISOL IN SHEEP

1979 ◽  
Vol 81 (1) ◽  
pp. 135-141 ◽  
Author(s):  
W. J. FULKERSON ◽  
B. Y. TANG
Keyword(s):  
Plasma Concentration ◽  
Circadian Rhythms ◽  
Plasma Level ◽  
Mean Value ◽  
Diurnal Variations ◽  
Ultradian Rhythm ◽  
Plasma Samples ◽  
True Rate ◽  
Daily Rate ◽  
Venous Cannula

Three Merino ewes, adapted for about 3 weeks to their environment, were bled at 10 min intervals through a jugular venous cannula. Radioimmunoassay of plasma samples for cortisol revealed marked diurnal variations with peak levels just after midnight and lowest values in the afternoon. This rhythm appeared to result from a changing amplitude associated with a distinct ultradian rhythm (frequency 0·8–1·2 cycles/h) in the plasma level of cortisol. Calculation of the daily rate of secretion of cortisol from the hormone profiles gave a mean value of 8·49 mg. Arguments are put forward in favour of this method for obtaining the true rate of secretion of cortisol.

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