THE MEASUREMENT OF PLASMA OESTRADIOL AND PROGESTERONE IN WOMEN WITH AMENORRHOEA TREATED WITH GONADOTROPHINS

1972 ◽  
Vol 69 (4) ◽  
pp. 608-616 ◽  
Author(s):  
D. M. Robertson ◽  
S. J. Steele
Keyword(s):  
Luteal Phase ◽  
Follicular Phase ◽  
Competitive Binding ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Plasma Progesterone ◽  
Infertile Women ◽  
Human Menopausal Gonadotrophin ◽  
Plasma Oestradiol

ABSTRACT Oestradiol and progesterone were measured in plasma of infertile women treated with human menopausal gonadotrophin (HMG) and human chorionic gonadotrophin (HCG). A total of 27 courses of treatment from 4 women were investigated. Plasma oestradiol was determined by a double competitive binding method and progesterone by competitive binding to corticosterone binding globulin. A relationship exists between the levels of plasma oestradiol in the follicular phase and the occurrence of ovulation as determined by plasma progesterone measurements in the luteal phase. Ovulation was not detected unless the oestradiol levels were in excess of 30 ng/100 ml plasma on the 7th day of treatment. The value of monitoring women undergoing gonadotrophin treatment by this procedure is discussed.

DETECTION OF CHORIONIC GONADOTROPHIN LIKE ACTIVITY IN INFERTILE CYCLES WITH A SHORT LUTEAL PHASE

1978 ◽  
Vol 88 (1) ◽  
pp. 164-168 ◽  
Author(s):  
M. Seppälä ◽  
P. Lehtovirta ◽  
E.-M. Rutanen
Keyword(s):  
Body Temperature ◽  
Luteinizing Hormone ◽  
Basal Body ◽  
Luteal Phase ◽  
Chorionic Gonadotrophin ◽  
Beta Subunit ◽  
Progesterone Concentration ◽  
Basal Body Temperature ◽  
Plasma Progesterone ◽  
Infertile Women

ABSTRACT Three of 24 infertile women with a short luteal phase transiently showed chorionic gonadotrophin (HCG) like immunoreactivity in serum as measured by the HCG-beta subunit radioimmunoassay. The plasma progesterone concentration was elevated above the lower normal post-ovulatory level in 2 of these women who also had elevated basal body temperature at the time HCG was detected. Positive HCG reaction coincided with the pre-ovulatory surge of luteinizing hormone (LH) in one patient, but in 2 other cases the LH concentration was low suggesting absence of LH interference. Our results suggest that some infertile women may conceive during their cycles with a short luteal phase and maintain pregnancy until HCG becomes detectable.

Induction of puberty in gilts 3. Ovulation, plasma oestradiol and progesterone in gilts injected with pregnant mare's serum gonadotrophin and human chorionic gonadotrophin

1981 ◽  
Vol 32 (1) ◽  
pp. 55-59 ◽  
Author(s):  
A. M. Paterson ◽  
G. B. Martin
Keyword(s):  
Luteal Phase ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Corpora Lutea ◽  
Progesterone Concentration ◽  
Cyclic Activity ◽  
Vulval Development ◽  
Subsequent Rise ◽  
Plasma Oestradiol ◽  
Second Peak

ABSTRACTNineteen prepubertal gilts were injected with a combination of 400 international units pregnant mare's serum gonadotrophin and 200 international units human chorionic gonadotrophin. Blood was sampled throughout the ensuing oestrous cycle and assayed for progesterone and oestradiol-17β.Eighteen gilts had vulval development consistent with oestrus within 5 days and had ovulated by day 12. Oestradiol levels in the plasma were elevated between days 2 and 6, and progesterone concentration increased between days 6 and 10 due to the establishment of functional corpora lutea. Progesterone concentration in the luteal phase was related to ovulation rate (r = 0·72, P<0·001).Three types of cyclic activity followed the luteal phase and a characteristic hormone profile was established for each. Five gilts exhibited a second ovulation accompanied by signs of oestrus. They had a second peak of oestradiol around days 24 to 26 and a subsequent rise in progesterone concentration. Four gilts displayed vulval development at this time but did not ovulate. A peak of oestradiol was detected. The remaining gilts did not display oestrus, nor did they ovulate. Oestradiol and progesterone concentrations remained basal in these gilts. The ovaries of prepubertal gilts treated with this combination of gonadotrophins produce normal patterns and levels of oestradiol and progesterone. Cyclic activity is not always maintained, probably due to differences in the maturity of the hypothalamic-pituitary-ovarian axis.

THE MEASUREMENT OF OESTRADIOL AND PROGESTERONE IN PLASMA FROM NORMAL, INFERTILE AND CLOMIPHENE TREATED WOMEN

1971 ◽  
Vol 68 (3) ◽  
pp. 523-533 ◽  
Author(s):  
D. M. Robertson ◽  
J. Mešter ◽  
A. E. Kellie
Keyword(s):  
Luteal Phase ◽  
Twin Pregnancy ◽  
Plasma Sample ◽  
Ovarian Function ◽  
Competitive Binding ◽  
Infertile Women ◽  
Corticosteroid Binding Globulin ◽  
Plasma Hormone ◽  
Plasma Oestradiol

ABSTRACT The use of clomiphene to assess ovarian function in women has been monitored by the determination of plasma oestradiol and progesterone concentrations. The assay of both hormones was carried out on one plasma sample after separation of oestradiol and progesterone by TLC. The oestradiol was determined by double competitive binding to rabbit uterine supernatant (Robertson et al. 1971) and the progesterone by competitive binding to corticosteroid binding globulin. After clomiphene stimulation the oestradiol levels in the follicular and luteal phases exceeded those found in normal cycles; this was also true of progesterone concentrations during the luteal phase. The methods of assay were also used to follow changes in plasma hormone levels during the early stages of pregnancy in hitherto infertile women. These cases included a twin pregnancy and a pregnancy which terminated in abortion at 7 weeks.

Endocrine response to superovulatory treatment in pre-pubertal beef heifers: its lack of ability to induce puberty

1988 ◽  
Vol 46 (2) ◽  
pp. 163-167 ◽  
Author(s):  
J. J. Bonavera ◽  
D. J. Tortonese ◽  
J. M. Doray ◽  
F. Petraglia
Keyword(s):  
Luteal Phase ◽  
Single Injection ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Ovarian Activity ◽  
Corpora Lutea ◽  
Strongly Correlated ◽  
Beef Heifers ◽  
Endocrine Response ◽  
Plasma Oestradiol

AbstractThis study was carried out to investigate: (1) changes in plasma oestradiol-17β and progesterone concentrations in six pre-pubertal beef heifers, 9 months old, subjected to a superovulatory treatment, consisting of a single dose of PMSG followed 120 h later by a single injection of human chorionic gonadotrophin (HCG); and (2) the ability of this treatment to trigger cyclic gonadal activity. After PMSG administration, oestradiol-17β levels increased from 11·8 (s.d. 10·78) to 92·9 (s.d. 38·15) ng/1 just before HCG injection. The oestradiol-17β increase during the superovulatory treatment and numbe r of ovulations were correlated (r = 0·83; P < 0·05). After HCG injection, progesterone concentrations rose significantly reaching values which in some cases were higher than 60 [μg/1. The progesterone peak on days 9 and 10 post HCG was strongly correlated with the number of corpora lutea (r = 0·95; P < 0·01 and r = 0·92; P < 0·01, respectively). Progesterone determinations were performed for a period of 90 days after HCG administration. No evidence was found for the induction of permanent cyclic ovarian activity since all heifers returned, after the induced luteal phase, to their pre-pubertal condition.

THE RELATION BETWEEN PLASMA PROGESTERONE AND TOTAL URINARY OESTROGENS FOLLOWING INDUCTION OF OVULATION IN WOMEN

1969 ◽  
Vol 62 (1) ◽  
pp. 89-97 ◽  
Author(s):  
Elof D. B. Johansson ◽  
Carl Gemzell
Keyword(s):  
Menstrual Cycle ◽  
Luteal Phase ◽  
Single Injection ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Human Pituitary ◽  
Plasma Progesterone ◽  
Before And After ◽  
Normal Menstrual Cycle ◽  
Different Levels

ABSTRACT Plasma progesterone levels have been measured before and after a single injection of 9000 IU of human chorionic gonadotrophin (HCG)* at different levels of total oestrogen excretion (TE) in 67 treatments with human pituitary gonadotrophin (HPG) in amenorrhoic women. With a TE excretion of less than 70 μg/24 h the rise in plasma progesterone was similar to that found in a normal menstrual cycle indicating the formation of only one corpus luteum. With more than 70 μg/24 h of TE several corpus lutea was formed as judged by the rise in plasma progesterone levels. No further increase was seen above 300 μg/24 h. The luteal phase was shorter than normal in the group with 75–300 μg/24 h of TE.

STIMULATION BY HUMAN CHORIONIC GONADOTROPHIN OF OESTRADIOL PRODUCTION BY DISPERSED CELLS FROM HUMAN CORPUS LUTEUM: COMPARISON WITH PROGESTERONE PRODUCTION; UTILIZATION OF EXOGENOUS TESTOSTERONE

1981 ◽  
Vol 91 (2) ◽  
pp. 197-203 ◽  
Author(s):  
M. C. RICHARDSON ◽  
G. M. MASSON
Keyword(s):  
Luteal Phase ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Cell Suspensions ◽  
Corpora Lutea ◽  
Progesterone Production ◽  
Late Luteal Phase ◽  
Oestradiol Production ◽  
Dispersed Cells

Cell suspensions were prepared from tissue samples of human corpora lutea obtained during the mid- and late-luteal phase of the menstrual cycle. Both oestradiol and progesterone production by dispersed cells were stimulated by similar concentrations of human chorionic gonadotrophin (hCG). As the degree of stimulation of production by hCG was greater for progesterone than for oestradiol (five- to tenfold compared with two- to threefold higher than basal production), the ratio of progesterone to oestradiol produced varied according to the level of trophic stimulation. A comparison of cell suspensions prepared from mid- and late-luteal phase corpora lutea, exposed to the same concentration of hCG (10 i.u./ml) in vitro, did not reveal a shift to oestradiol production in the late-luteal phase. Provision of additional testosterone during incubation raised the level of oestradiol production by dispersed luteal cells. At an optimum concentration of testosterone (1 μmol/l), oestradiol synthesis was not raised further in the presence of hCG or N6, O2-dibutyryl cyclic AMP, suggesting a lack of induction or activation of the aromatase system by gonadotrophin in short-term cultures. Basal and stimulated levels of progesterone production were not significantly impaired in the presence of testosterone.

ANTIGONADOTROPHIC PROFILE OF ANTISERA AGAINST HUMAN GONADOTROPHIN PREPARATIONS

1971 ◽  
Vol 67 (2) ◽  
pp. 262-276 ◽  
Author(s):  
P. Petrusz ◽  
C. Robyn ◽  
E. Diczfalusy
Keyword(s):  
Biological Activity ◽  
Luteinizing Hormone ◽  
Total Dose ◽  
Follicle Stimulating Hormone ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Immunological Activity ◽  
Human Menopausal Gonadotrophin ◽  
Stimulating Hormone

ABSTRACT Forty-two antisera were prepared in rabbits against human chorionic gonadotrophin (HCG), human hypophysial gonadotrophin (HHG), human urinary luteinizing hormone (LH) and human menopausal gonadotrophin (HMG) preparations. The gonadotrophic profiles of the antigens were previously characterized by bioassay, immunoassay and bioimmunoassay methods. The 25 most potent antisera were tested in statistically valid bioassays for their HCG and follicle stimulating hormone (FSH) neutralizing activities as well as for their neutralizing potencies against the FSH-like activity present in HCG preparations. The anti-HCG/anti-FSH ratios of the anti-HCG sera tested varied between 6.2 and > 254, while those of the anti-HHG, anti-LH and anti-HMG sera were close to 2. It was found that the total dose of immunological activity (anti-HCG neutralizing and anti-FSH neutralizing potency) rather than that of the biological activity administered to the rabbits was decisive for obtaining antisera with high anti-HCG and anti-FSH titers. Immunization with a highly purified HCG preparation (> 17 000 IU/mg) resulted in antisera exhibiting lower anti-HCG/anti-FSH ratios than did immunization with partially purified preparations. A highly purified urinary LH preparation which did not contain any detectable FSH activity gave rise to antisera exhibiting anti-HCG/anti-FSH ratios of approximately 2.0. These highly purified HCG and LH preparations were shown previously to possess high anti-FSH neutralizing potencies (Petrusz et al. 1971b). Booster injections did not change significantly the quality or the titer of the antigonadotrophic sera studied. The HCG neutralizing potency of anti-HCG sera was approximately 3 times higher when assayed against a highly purified HCG preparation (> 17 000 IU/mg) as compared to potency estimates obtained against the laboratory standard of HCG (about 2000 IU/mg). It is suggested that consideration should be given to the establishment of standard preparations of antigonadotrophic sera. It is concluded that bioimmunoassays are more suitably than conventional bioassay methods for the assessment of the antigenic purity of human gonadotrophin preparations.

Sign in / Sign up

Export Citation Format

Share Document