THE INFLUENCE OF CYPROTERONE ACETATE ON THE UPTAKE OF TESTOSTERONE AND ON THE DNA- AND RNA-AMOUNT IN LIVER, PROSTATE AND SEMINAL VESICLES OF IMMATURE MALE RATS IN VIVO

1969 ◽  
Vol 61 (1_Suppl) ◽  
pp. S43 ◽  
Author(s):  
E. Jäger ◽  
K.-O. Mosebach ◽  
H.-P. Blumenthal ◽  
A. Scheuer
Keyword(s):  
Cyproterone Acetate ◽  
Seminal Vesicles ◽  
Male Rats ◽  
Immature Male ◽  
Dna And Rna

scholarly journals CONCENTRATION OF THE GONADOTROPIC HORMONE IN PREGNANT MARE'S SERUM

1933 ◽  
Vol 58 (5) ◽  
pp. 569-574 ◽  
Author(s):  
Herbert M. Evans ◽  
Edwin L. Gustus ◽  
Miriam E. Simpson
Keyword(s):  
Aluminum Hydroxide ◽  
Physiological Saline ◽  
Seminal Vesicles ◽  
Male Rats ◽  
Subcutaneous Injections ◽  
Gonadotropic Hormone ◽  
Female Mice ◽  
Immature Male ◽  
Active Aluminum

The gonadotropic hormone of the blood of the pregnant mare has been greatly concentrated by adsorption on active aluminum hydroxide followed by elution. The preparations so obtained gave demonstrable gonadotropic effects within 100 hours in 21 day old female mice following three subcutaneous injections of 0.001 mg. in 1 cc. of physiological saline. As is well known, other gonadotropic substances do not cause conspicuous development of the male gonads but injections of comparatively large doses of these preparations into immature male rats caused marked development of the testes, which in 10 days were trebled in weight. An astonishing increase in the weight of the seminal vesicles resulted, for these organs were approximately 75 times heavier than in controls.

AN AUTORADIOGRAPHIC STUDY ON THE LOCALIZATION OF ANDROGEN IN THE PROSTATE GLAND AND THE SEMINAL VESICLES OF THE MALE RAT

1970 ◽  
Vol 63 (2) ◽  
pp. 207-215 ◽  
Author(s):  
Kjell J. Tveter
Keyword(s):  
Epithelial Cells ◽  
Prostate Gland ◽  
Autoradiographic Study ◽  
Seminal Vesicles ◽  
Radioactive Material ◽  
Male Rats ◽  
Male Rat ◽  
Selective Localization ◽  
Qualitative Pattern

ABSTRACT The distribution of radioactive material in the prostate gland and the seminal vesicles has been studied by autoradiography after intramuscular administration of [1,2-3H] testosterone in vivo to adult castrated male rats. Positive autoradiographs were obtained from 7½ min to 8 h after the administration. As early as after 15 min, there appeared to be a selective localization of radioactivity in the epithelial cells, with much of the labelling associated with the nuclei; the stromal labelling was markedly less. This picture was even more significant ½, 1 and 2 h after the injection, when the autoradiographs demonstrated a preferential labelling of the nuclei of the epithelial cells. A distinct labelling of the epithelial cells was also found 8 h after the injection. The same qualitative pattern of distribution of radioactivity was seen in the four prostatic lobes and the seminal vesicles. No significant labelling of the secretions in the glandular lumina was observed.

A COMPARISON OF SEMINAL VESICLE, KIDNEY AND ADRENAL RESPONSES IN THE RAT FOLLOWING THE ADMINISTRATION OF ANDROGEN

1960 ◽  
Vol XXXIV (III) ◽  
pp. 344-352 ◽  
Author(s):  
Jerome A. Grunt ◽  
Joseph E. Walker
Keyword(s):  
Seminal Vesicle ◽  
Histological Examination ◽  
Testosterone Propionate ◽  
Seminal Vesicles ◽  
Sesame Oil ◽  
Male Rats ◽  
Subcutaneous Injections ◽  
Sexual Organ ◽  
Seminal Vesicle Weight

ABSTRACT The responses of several seminal vesicle in vivo microscopic characteristics, seminal vesicle contractions, seminal vesicle, kidney and adrenal weights and seminal vesicle histology have been compared following the administration of testosterone propionate (T. P.) and norethandrolone (19-NT) to castrated male rats. Fourteen days after castration 35 male Osborne-Mendel rats were divided into 5 groups each containing 6 to 8 animals and given daily subcutaneous injections of 0.1 ml sesame oil with or without added steroids. The groups were: I – sesame oil; II – 100 μg 19-NT; III – 500 μg 19-NT; IV – 100 μg T.P.; V – 500 μg T. P. After 7 days of injections, seminal vesicles were studied by the transillumination method of Knisely. The seminal vesicles, adrenals and kidneys were then removed and weighed and the vesicles were prepared for routine histological examination. The adrenal and kidney weights, as well as the seminal vesicle contractions, responded to both steroids in a similar manner, however, seminal vesicle weight and histology and in vivo characteristics differed greatly. 19-NT appeared to have very little effect on vesicle weight, in vivo characteristics and histology although contractions were inhibited in 13 of 14 animals so treated. Therefore, when used as the end-point in hormonal reactions, seminal vesicle contractions should not be considered comparable to the usual secondary sexual organ reactivity to hormones.

MORPHOLOGICAL AND BEHAVIOURAL EFFECTS OF AN 'ANTIANDROGEN' IN MALE RATS

1968 ◽  
Vol 42 (3) ◽  
pp. 379-NP ◽  
Author(s):  
F. A. BEACH ◽  
W. H. WESTBROOK
Keyword(s):  
Cyproterone Acetate ◽  
Microscopic Analysis ◽  
Experimental Treatment ◽  
Seminal Vesicles ◽  
Male Rats ◽  
Glans Penis ◽  
Detectable Effect ◽  
Behavioural Effects ◽  
Mating Tests ◽  
Copulatory Behaviour

SUMMARY Male rats injected with 10 mg. cyproterone acetate daily for 28 days were given weekly mating tests with receptive females. At the conclusion of testing the animals were killed and the testes and seminal vesicles were weighed. These organs and the glans penis were then prepared for microscopic analysis. The experimental treatment had no detectable effect upon copulatory behaviour nor upon the testis or the cornified papillae in the integument of the glans penis. The seminal vesicles of treated males appeared to be non-secretory and were lighter than those of control rats but heavier than the vesicles of untreated castrates. It is concluded that although cyproterone acetate partially blocked the action of endogenous androgen on the seminal vesicles it had neither a blocking effect on the penile papillae nor on the central nervous mechanisms which mediate mating responses.

METABOLISM IN VIVO OF [3H] ANDROSTENEDIONE AND [3H] TESTOSTERONE BY ANDROGEN DEPENDENT TISSUES

1970 ◽  
Vol 65 (4) ◽  
pp. 723-730 ◽  
Author(s):  
Kjell J. Tveter ◽  
Asbjörn Aakvaag
Keyword(s):  
Intramuscular Injection ◽  
Total Activity ◽  
Seminal Vesicles ◽  
Radioactive Material ◽  
Male Rats ◽  
Ventral Prostate ◽  
Supernatant Fraction ◽  
Rectus Abdominis Muscle ◽  
Main Metabolite

ABSTRACT The radioactive material present in the different prostatic lobes and the seminal vesicles was isolated and identified after intramuscular injection of [1,2-3H] testosterone to adult castrated male rats. 5α-Dihydrotestosterone was the main metabolite, representing 70, 72, 49, 56 and 70%, respectively, of the total activity in the ventral, dorsal and lateral prostate, the coagulating glands and the seminal vesicles one hour after the administration of hormone. The corresponding values for unchanged [3H] testosterone were 16, 6.4, 23, 6 and 15%, respectively. In rectus abdominis muscle less than 0.3% was 5α-dihydrotestosterone, while 37% represented unconverted [3H] testosterone. Of the activity in liver, [3H]-testosterone accounted for 0.2%, whereas less than 0.1% was 5α-dihydrotestosterone. One hour after administration of [1,2-3H] androst-4-ene-3,17-dione to adult castrated rats, the uptake of radioactivity in the ventral prostate was about 2.7 times higher than in skeletal muscle. In the ventral prostate, 32% of the total activity present at this time was represented by 5α-dihydrotestosterone, while 3.5% was unmetabolized [3H] androstenedione. The corresponding values for the seminal vesicles were 24 and 3.7%, respectively. In the 105 000 × g supernatant fraction of homogenized ventral prostate tissue, part of the radioactivity was associated with soluble macromolecules one hour after the administration of [3H]-androstenedione.

BINDING AND METABOLISM OF 5α-ANDROSTANE-3α,17β-DIOL AND OF 5α-ANDROSTANE-3β,17β-DIOL IN THE PROSTATE, SEMINAL VESICLES AND PLASMA OF MALE RATS: STUDIES IN VIVO AND IN VITRO

1975 ◽  
Vol 64 (3) ◽  
pp. 529-538 ◽  
Author(s):  
M. KRIEG ◽  
H.-J. HORST ◽  
M.-L. STERBA
Keyword(s):  
Skeletal Muscle ◽  
Specific Binding ◽  
Biological Effects ◽  
Sucrose Density Gradient ◽  
Total Radioactivity ◽  
Seminal Vesicles ◽  
Male Rats ◽  
Cytosol Fraction

SUMMARY Binding of 5α-androstane-3α,17β-diol (3α-diol) and 5α-androstane-3β,17β-diol (3β-diol) in vivo and in vitro to the 100000 g cytosol fraction of the rat prostate and seminal vesicles as well as to plasma was studied by agargel electrophoresis and sucrose density gradient ultracentrifugation and the results compared with the corresponding findings for 5α-dihydrotestosterone (5α-DHT). The metabolism of 3α-diol and 3β-diol was also investigated by thin-layer chromatography. The following results were obtained: (1) A specific binding of 3α-diol and 3β-diol by the cytosols could not be demonstrated in vitro, while 5α-DHT was specifically bound. (2) In plasma, 3α-diol was extensively bound, 3β-diol less extensively bound, while 5α-DHT remained unbound. (3) After intravenous injection of 3α-diol, specifically bound radioactivity, increasing within 30 min, was found in the prostate cytosol, while after 3β-diol injection no binding occurred. (4) Parallel to the increased binding, the total radioactivity in the prostate accumulated within 30 min after 3α-diol injection, the uptake being 5·3 times higher than in skeletal muscle. However after 3β-diol injection, total radioactivity decreased in the prostate within 30 min, the uptake being only 1·5 times higher than in skeletal muscle. (5) One minute after injection of 3α-diol, 53% of the extracted radioactivity in the prostate had been converted to 5α-DHT, this increased within 30 min to 81%. Thirty minutes after the injection of 3β-diol, about 32% of the extracted radioactivity in the prostate had been converted to 5α-DHT. (6) From the in-vivo and in-vitro experiments it was concluded that 3α-diol exerts its biological effects mainly by its conversion into 5α-DHT.

scholarly journals Herbicide Metolachlor Causes Changes in Reproductive Endocrinology of Male Wistar Rats

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Francielle Tatiane Mathias ◽  
Renata Marino Romano ◽  
Hanan Kaled Sleiman ◽  
Claudio Alvarenga de Oliveira ◽  
Marco Aurelio Romano
Keyword(s):  
Reproductive Toxicity ◽  
Seminal Vesicles ◽  
Seminiferous Epithelium ◽  
Male Rats ◽  
Serum Concentrations ◽  
Reproductive Endocrinology ◽  
Male Wistar Rats ◽  
Herbicide Metolachlor ◽  
Morphometric Evaluation

S-metolachlor is a chloroacetanilide herbicide widely used in the agriculture to control weeds and was demonstrated that it increases the activity of the aromatase enzyme in cell cultures, which may culminate as endocrine disruption action in vivo. To investigate this hypothesis, prepubertal Wistar male rats were exposed to metolachlor (5 or 50 mg/kg/day, NOEL for reproductive toxicity: 23.5–26.0 mg/kg/day) from PND23 (postnatal day) to PND53. During this period, the growth of the animals and the age and weight at puberty were recorded. In PND53, tissues were collected and the analysis of LH, FSH, testosterone, dihydrotestosterone (DHT), estradiol serum concentrations, morphometric evaluation of the seminiferous epithelium, and weight of the testes and the seminal vesicle (undrained and drained) was performed (Statistical difference: P<0.05). Metolachlor caused an increase in serum concentrations of testosterone, estradiol, and FSH and a reduction in DHT but did not alter the LH. There were also observed a higher amount of fluid in the seminal vesicles, precocious puberty, and changes in morphology of the seminiferous epithelium of treated animals. We demonstrated in this paper that prepubertal exposure to S-metolachlor caused changes in reproductive endocrinology of male rats.

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