A METHOD FOR THE ESTIMATION OF INDIVIDUAL URINARY CORTICOSTEROIDS, PREGNANEDIOL, PREGNANETRIOL AND PREGNANETRIOLONE FOLLOWING THEIR SEPARATION BY THIN-LAYER CHROMATOGRAPHY

1969 ◽  
Vol 61 (1) ◽  
pp. 1-16 ◽  
Author(s):  
Z. Kraiem ◽  
B. Lunenfeld ◽  
J. Skalka
Keyword(s):  
Enzymatic Hydrolysis ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Sulphuric Acid ◽  
Urine Sample ◽  
Blue Tetrazolium ◽  
Acid Reaction ◽  
Colorimetric Estimation ◽  
The Individual ◽  
Layer Chromatography

ABSTRACT A method is described for estimating urinary pregnanediol, pregnanetriol, pregnanetriolone and the following individual corticosteroids: tetrahydrocortisol (THF), allotetrahydrocortisol (allo-THF), tetrahydrocortisone (THE), cortisol (F), tetrahydro-11-deoxycortisol (THS), tetrahydrocorticosterone (THB), allotetrahydrocorticosterone (allo-THB), tetrahydro-11-dehydrocorticosterone (THA), tetrahydro-11-deoxycorticosterone (THDOC). The main steps of the method are: enzymatic hydrolysis, extraction, fractionation by thin-layer chromatography, and colorimetric estimation using the sulphuric acid reaction for pregnanediol, pregnanetriol and pregnanetriolone, and the blue tetrazolium reaction for the individual corticosteroids. The reliability criteria (specificity, accuracy, precision and sensitivity) of the method have been assessed. The advantage of the method lies in its practicability since, unlike other methods: a) the same urine sample (50 ml) is submitted, for the estimation of the twelve C21 steroids, to the same procedure until fractionation, and b) thin-layer chromatography alone is used for the latter purpose.

Single-pH Extraction Procedure for Detecting Drugs of Abuse

1974 ◽  
Vol 20 (2) ◽  
pp. 309-311 ◽  
Author(s):  
Ramon E Stoner ◽  
Connie Parker
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Drugs Of Abuse ◽  
Clinical Laboratory ◽  
Extraction Procedure ◽  
Organic Salt ◽  
Basic Drugs ◽  
Laboratory Service ◽  
The Individual ◽  
Layer Chromatography

Abstract A method is presented for determining certain drugs of abuse in a single extract of urine. The urine sample is adjusted to pH 6.0 with a buffer containing bromcresol purple. Basic drugs, such as amphetamines and narcotics, form an organic salt with the ionized bromcresol purple, which is extractable with a mixture of chloroform and 2-propanol (3:1 by volume). At pH 6.0, weak acids such as barbiturates and neutral drugs such as glutethimide are also soluble in this solvent. Consequently, the major classes of drugs are extracted simultaneously. The extract is then concentrated and the individual drugs are determined by thin-layer chromatography in a solvent that will separate bromcresol purple from the drugs. Additional Keyphrases: drug screening #{ 149} toxicology #{ 149} thin-layer chromatography #{ 149} screening procedure Clinical Laboratory Service, West Side VA Hosp., 820 S. Damen Ave., Chicago, Ill. 60612.

Analysis of Trisulfapyrimidine Preparations by Thin Layer Chromatography

1972 ◽  
Vol 55 (1) ◽  
pp. 194-196
Author(s):  
Charlotte A Brunner
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Collaborative Study ◽  
Colorimetric Measurement ◽  
Intact Sample ◽  
The Individual ◽  
Layer Chromatography

Abstract A method for the analysis of trisulfapyrimidine preparations is described. The total sulfonamide content is measured on the intact sample, and the ratio of the components is determined by colorimetric measurement of the individual compounds after separation by thin layer chromatography. A collaborative study will be performed.

DIE FLUOROMETRISCHE BESTIMMUNG VON TESTOSTERON, EPITESTOSTERON UND ANDROST-4-EN-3,17-DION NACH DÜNNSCHICHTCHROMATOGRAPHISCHER ISOLIERUNG AUS DEM HARN

1968 ◽  
Vol 58 (3) ◽  
pp. 353-363 ◽  
Author(s):  
W. Hubl ◽  
K. Schollberg
Keyword(s):  
Enzymatic Hydrolysis ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Complete Separation ◽  
The Other ◽  
Simple Method ◽  
Layer Chromatography

ABSTRACT The authors describe a simple method for determining testosterone, epitestosterone and androstenedione. The method employs enzymatic hydrolysis for glucuronide conjugates, Girard-partition, thin-layer chromatography using alumina and polyamide and fluorometric quantitation of the eluted steroids. By means of TLC on polyamide it is possible to separate (testosterone + epitestosterone) from the other C19-steroids. The complete separation of testosterone from epitestosterone is obtained by TLC on alumina as well as on silicagel G.

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