DIE FLUOROMETRISCHE BESTIMMUNG VON TESTOSTERON, EPITESTOSTERON UND ANDROST-4-EN-3,17-DION NACH DÜNNSCHICHTCHROMATOGRAPHISCHER ISOLIERUNG AUS DEM HARN

1968 ◽  
Vol 58 (3) ◽  
pp. 353-363 ◽  
Author(s):  
W. Hubl ◽  
K. Schollberg
Keyword(s):  
Enzymatic Hydrolysis ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Complete Separation ◽  
The Other ◽  
Simple Method ◽  
Layer Chromatography

ABSTRACT The authors describe a simple method for determining testosterone, epitestosterone and androstenedione. The method employs enzymatic hydrolysis for glucuronide conjugates, Girard-partition, thin-layer chromatography using alumina and polyamide and fluorometric quantitation of the eluted steroids. By means of TLC on polyamide it is possible to separate (testosterone + epitestosterone) from the other C19-steroids. The complete separation of testosterone from epitestosterone is obtained by TLC on alumina as well as on silicagel G.

Determination of Functionality Distributions in Telechelic Prepolymers by Thin-Layer Chromatography

1977 ◽  
Vol 50 (1) ◽  
pp. 63-70 ◽  
Author(s):  
T. I. Min ◽  
T. Miyamoto ◽  
H. Inagaki
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Complete Separation ◽  
Hydroxyl Groups ◽  
Simple Method ◽  
Flame Ionisation Detector ◽  
Layer Chromatography ◽  
Flame Ionisation ◽  
Ionisation Detector

Abstract Functionality distributions of telechelic prepolymers have been determined by thin layer chromatography (TLC). Commercially available 1,2-polybutadienes having either carboxyl or hydroxyl groups were examined. TLC with p-xylene as the developer made it possible to separate the sample into a nonfunctional component and a mixture of mono- and difunctional components. Complete separation of the sample into the three components was achieved by selecting the developer and development procedure appropriately. Quantification of the chromatograms was performed successfully in a TLC apparatus equipped with a flame ionisation detector. A simple method for the determination of the functionality distribution was proposed.

Simple Method for Determination of Glycine and Niacin in Drug Mixtures

1968 ◽  
Vol 51 (1) ◽  
pp. 11-14
Author(s):  
Keith L Egli ◽  
Anthony Romano
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Ultraviolet Absorption ◽  
Simple Method ◽  
Drug Mixtures ◽  
Layer Chromatography

Abstract A method, based on the formol titration of glycine and the ultraviolet absorption of niacin, has been developed for the determination of these compounds in drug mixtures. Neither compound requires isolation for the determination. Glycine and niacin are identified by thin layer chromatography

Sugar-meal sources used by female black flies (Diptera: Simuliidae): a four-habitat study

1997 ◽  
Vol 75 (7) ◽  
pp. 1066-1072 ◽  
Author(s):  
Steven G. Burgin ◽  
Fiona F. Hunter
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
The Other ◽  
Black Flies ◽  
Layer Chromatography ◽  
Provincial Park

Adult black flies were sampled by sweep-netting vegetation in four habitats within Algonquin Provincial Park, Ontario: Davies Bog, the airfield, deciduous habitat, and coniferous habitat. Sugars in the crops and midguts of female flies (n = 773) were tested by thin-layer chromatography to determine whether the flies had fed on nectar or homopteran honeydew. Melezitose and stachyose were used as honeydew-indicator sugars. For Simulium venustum, it was found that significantly fewer black flies (19%) from the airfield contained honeydew sugars than black flies from the other three sites (34% from Davies Bog; 36% from deciduous habitat; 25% from coniferous habitat). We argue that black flies will feed on nectar or honeydew according to availability.

scholarly journals 5α-Androstane-3α,17β-Diol Is Formed in Tammar Wallaby Pouch Young Testes by a Pathway Involving 5α-Pregnane-3α,17α-Diol-20-One as a Key Intermediate

Endocrinology ◽  
2003 ◽  
Vol 144 (2) ◽  
pp. 575-580 ◽  
Author(s):  
Jean D. Wilson ◽  
Richard J. Auchus ◽  
Michael W. Leihy ◽  
Oleg L. Guryev ◽  
Ronald W. Estabrook ◽  
...  
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Reductase Inhibitor ◽  
Tammar Wallaby ◽  
The Other ◽  
Synthetic Pathway ◽  
D 20 ◽  
17 Hydroxyprogesterone ◽  
Layer Chromatography

The synthetic pathway by which 5α-androstane-3α,17β-diol (5α-adiol) is formed in the testes of tammar wallaby pouch young was investigated by incubating testes from d 20–40 males with various radioactive precursors and analyzing the metabolites by thin-layer chromatography and HPLC. [3H]Progesterone was converted to 17-hydroxyprogesterone, which was converted to 5α-adiol by two pathways: One involves the formation of testosterone and dihydrotestosterone as intermediates, and the other involves formation of 5α-pregnane-3α,17α-diol-20-one (5α-pdiol) and androsterone as intermediates. Formation of 5α-adiol from both [3H]testosterone and [3H]progesterone was blocked by the 5α-reductase inhibitor 4MA. The addition of nonradioactive 5α-pdiol blocked the conversion of [3H]progesterone to 5α-adiol, and [3H]5α-pdiol was efficiently converted to androsterone and 5α-adiol. We conclude that expression of steroid 5α-reductase in the developing wallaby testes allows formation of 5α-reduced androgens by a pathway that does not involve testosterone as an intermediate.

A complete separation of lipids by three-directional thin layer chromatography

Lipids ◽  
1983 ◽  
Vol 18 (12) ◽  
pp. 896-899 ◽  
Author(s):  
J. K. G. Kramer ◽  
R. C. Fouchard ◽  
E. R. Farnworth
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Complete Separation ◽  
Layer Chromatography

A METHOD FOR THE ESTIMATION OF INDIVIDUAL URINARY CORTICOSTEROIDS, PREGNANEDIOL, PREGNANETRIOL AND PREGNANETRIOLONE FOLLOWING THEIR SEPARATION BY THIN-LAYER CHROMATOGRAPHY

1969 ◽  
Vol 61 (1) ◽  
pp. 1-16 ◽  
Author(s):  
Z. Kraiem ◽  
B. Lunenfeld ◽  
J. Skalka
Keyword(s):  
Enzymatic Hydrolysis ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Sulphuric Acid ◽  
Urine Sample ◽  
Blue Tetrazolium ◽  
Acid Reaction ◽  
Colorimetric Estimation ◽  
The Individual ◽  
Layer Chromatography

ABSTRACT A method is described for estimating urinary pregnanediol, pregnanetriol, pregnanetriolone and the following individual corticosteroids: tetrahydrocortisol (THF), allotetrahydrocortisol (allo-THF), tetrahydrocortisone (THE), cortisol (F), tetrahydro-11-deoxycortisol (THS), tetrahydrocorticosterone (THB), allotetrahydrocorticosterone (allo-THB), tetrahydro-11-dehydrocorticosterone (THA), tetrahydro-11-deoxycorticosterone (THDOC). The main steps of the method are: enzymatic hydrolysis, extraction, fractionation by thin-layer chromatography, and colorimetric estimation using the sulphuric acid reaction for pregnanediol, pregnanetriol and pregnanetriolone, and the blue tetrazolium reaction for the individual corticosteroids. The reliability criteria (specificity, accuracy, precision and sensitivity) of the method have been assessed. The advantage of the method lies in its practicability since, unlike other methods: a) the same urine sample (50 ml) is submitted, for the estimation of the twelve C21 steroids, to the same procedure until fractionation, and b) thin-layer chromatography alone is used for the latter purpose.

Platelet Factor 3 Activity in Washed Platelets

1973 ◽  
Vol 30 (03) ◽  
pp. 557-566 ◽  
Author(s):  
S Renaud ◽  
P Gautheron ◽  
H Rosenstein
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Platelet Rich Plasma ◽  
The Other ◽  
Slow Speed ◽  
Platelet Factor ◽  
Platelet Poor Plasma ◽  
Platelet Counts ◽  
Edta Solution ◽  
Layer Chromatography

SummaryPlatelets collected with an EDTA solution and simply washed in an incomplete Tyrode’s presented clotting times in the recalcification (man and rat) and the Stypven (rat) tests that were practically identical to those of the PRP when slow speed centrifugation was used (800 G in man, 1000 G in rat). This was demonstrated, in 6 pools of 5 rats each and in 6 men, by comparing the clotting activity of the citrated platelet-rich plasma to that of the platelets washed and resuspended in the citrated platelet-poor plasma, for platelet counts ranging from 1 × 105 to 10 × 105/mm3. In contrast, centrifugation of platelets at 3000 G markedly affected these clotting activities, as was shown in an additional study comprising 6 pools of 3 rats.Finally, the clotting activity of platelets totally disrupted by sonication appears to be identical quantitatively in both man and rats to that of the total phospholipids extracted from these platelets and separated from the other lipids by thin-layer chromatography and resuspended in plasma by sonication.

Sign in / Sign up

Export Citation Format

Share Document