STUDIES ON THE DOSE-RESPONSE CURVES IN THE VENTRAL PROSTATE METHOD FOR LUTEINIZING HORMONE

1967 ◽  
Vol 56 (4) ◽  
pp. 608-618 ◽  
Author(s):  
Peter Christiansen
Keyword(s):  
Luteinizing Hormone ◽  
Dose Response ◽  
Human Chorionic Gonadotrophin ◽  
Ventral Prostate ◽  
Human Origin ◽  
Response Curves ◽  
Human Pituitary ◽  
Human Menopausal Gonadotrophin ◽  
Dose Response Curves ◽  
The One

ABSTRACT Six luteinizing hormone preparations of human, ovine and equine origin were assayed by a modification of Greep's ventral prostate method. The dose-response curves for human and equine preparations on the one hand and the ovine preparation on the other had a widely different course. The ovine preparation gave a flat curve and did not even in large dosis, exhibit prostatic weights of the magnitude obtained by human preparations. The human and equine preparations gave steep curves, equally steep for human chorionic gonadotrophin (HCG), human menopausal gonadotrophin (HMG), human pituitary gonadotrophin (HPG), and the equine preparation. The slope of the curve is unaffected by the route of administration of the hormones, subcutaneous or intraperitoneal. The causes of the differences in the course of the curves are discussed and the importance of using standards of human origin when testing hormonal substances from humans is pointed out.

OVARIAN ASCORBIC ACID DEPLETION TEST FOR LUTEINIZING HORMONE

1967 ◽  
Vol 56 (4) ◽  
pp. 619-625 ◽  
Author(s):  
Hans Jacob Koed ◽  
Christian Hamburger
Keyword(s):  
Ascorbic Acid ◽  
Luteinizing Hormone ◽  
Dose Response ◽  
Response Curve ◽  
Dose Response Curve ◽  
Human Origin ◽  
Response Curves ◽  
Significant Difference ◽  
The Mean ◽  
Different Levels

ABSTRACT Comparison of the dose-response curves for LH of ovine origin (NIH-LH-S8) and of human origin (IRP-HMG-2) using the OAAD test showed a small, though statistically significant difference, the dose-response curve for LH of human origin being a little flatter. Two standard curves for ovine LH obtained with 14 months' interval, were parallel but at different levels of ovarian ascorbic acid. When the mean ascorbic acid depletions were calculated as percentages of the control levels, the two curves for NIH-LH-S8 were identical. The use of standards of human origin in the OAAD test for LH activity of human preparations is recommended.

FURTHER OBSERVATIONS ON THE OVARIAN ASCORBIC ACID DEPLETION TEST FOR LUTEINIZING HORMONE

1965 ◽  
Vol 32 (1) ◽  
pp. 1-7 ◽  
Author(s):  
E. T. BELL ◽  
J. A. LORAINE ◽  
S. MUKERJI ◽  
PACHARA VISUTAKUL
Keyword(s):  
Ascorbic Acid ◽  
Luteinizing Hormone ◽  
Dose Response ◽  
Intravenous Route ◽  
Response Curves ◽  
Standard Preparation ◽  
Routes Of Administration ◽  
Seasonal Factors ◽  
Dose Response Curves ◽  
The Absolute

SUMMARY A modification of the ovarian ascorbic acid depletion (OAAD) method for luteinizing hormone (LH) is described in which the standard and test materials are administered by the intraperitoneal rather than by the intravenous route. The potency of NIH-LH when administered i.p. and i.v. was the same and the slopes of the log dose-response curves for the two routes of administration were very similar. Studies on the effect of seasonal factors on the OAAD method showed that both the slopes of the log dose-response curves and the absolute levels of ascorbic acid varied from one assay to another. The necessity for using a standard preparation in all assays of LH activity by the OAAD method is emphasized.

A Methodological Investigation On The One-Stage Factor VIII Assay

1981 ◽  
Author(s):  
J Over ◽  
J A van Mourik ◽  
P van den Brink-Zantingh ◽  
R Smit-Jansen
Keyword(s):  
Factor Viii ◽  
Dose Response ◽  
Red Cross ◽  
Response Curves ◽  
One Stage ◽  
Factor Viii Concentrates ◽  
Dose Response Curves ◽  
The One ◽  
Protein Rich

Assay of Factor VIII coagulant activity (VIII: C) in Factor VIII concentrates has since long met difficulties, such as l) non-paralleility of dose-response curves of plasma standard and Factor VIII concentrate, 2) spuriously low values of VIII: C in concentrates as revealed by abnormally high in vivo recoveries after transfusion, and 3) large interlaboratory variation in assay results. In an attempt to analyze the cause of these problems several parameters of the one-stage assay system were varied systematically and their effect on the parallellity of dose-response curves and on the final VIII: C value was analyzed. Nonparallel1ity was partially corrected with a protein-rich dilution medium, and almost always completely with undiluted instead of 1:1 diluted hemophilic substrate plasma. In both conditions apparently higher VIII:C values were found.A number of assay systems used by different producers of Factor VIII concentrates were compared. The standard and, in some cases, the phospholipid reagent seemed to contribute for the largest past to the inter1aboratory variation, but also other, as yet unidentified, factors exerted some influence. These findings initiated a cooperative study by five Red Cross Blood Transfusion Services in Europe on standardization of the one-stage assay for VIII:C. This resulted in a better correspondence between these institutes (CV 13%) compared to the previous situation (CV 23%).It is concluded that 1) substrate plasma should not be diluted, especially when Factor VIII concentrate is to be tested against a plasma standard, 2) the standard should be of the same type as the testmaterial, and 3) this standard should be properly calibrated against the International Standard for Factor VIII.

scholarly journals Studies on rat ovarian receptors for lutropin (luteinizing hormone). Applicability of radioimmunoassay to measure lutropin bound to receptors

1976 ◽  
Vol 160 (3) ◽  
pp. 603-606 ◽  
Author(s):  
K Muralidhar ◽  
N R Moudgal
Keyword(s):  
Luteinizing Hormone ◽  
Dose Response ◽  
Response Curves ◽  
Dose Response Curves

Measurement of receptor-bound unlabelled physiologically active lutropin (luteinizing hormone, LH) was possible by a modified radioimmunoassay. The conventional radioimmunoassay conducted at 4° C was inadequate, whereas the modified assay performed at 37° C could measure receptor-bound lutropin. The radioimmunoassay at 37° C takes only 36h for completion compared with 5-7 days at 4° C. The sensitivity and range of dose-response curves are, however, unaltered. The validity of the technique was established by a number of criteria.

IMMUNOLOGICAL CROSS REACTION BETWEEN HUMAN CHORIONIC GONADOTROPHIN AND HUMAN PITUITARY GONADOTROPHINS

1966 ◽  
Vol 53 (3) ◽  
pp. 420-428 ◽  
Author(s):  
C. Robyn ◽  
P. O. Hubinont ◽  
E. Diczfalusy
Keyword(s):  
Luteinizing Hormone ◽  
Follicle Stimulating Hormone ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Human Pituitary ◽  
Specific Antisera ◽  
Human Menopausal Gonadotrophin ◽  
Immunological Cross Reaction

ABSTRACT Immunologically mono-specific antisera prepared against human chorionic gonadotrophin (HCG) preparations completely neutralized in vitro as well as in vivo the luteinizing hormone (LH) and also the follicle-stimulating hormone (FSH) activity of both human hypophyseal gonadotrophin (HHG) and human menopausal gonadotrophin (HMG) preparations.

STUDIES ON URINARY GONADOTROPHINS

1959 ◽  
Vol XXXII (IV) ◽  
pp. 497-508 ◽  
Author(s):  
Svend G. Johnsen ◽  
Christian Hamburger
Keyword(s):  
Luteinizing Hormone ◽  
Follicle Stimulating Hormone ◽  
Qualitative Difference ◽  
The Other ◽  
Response Curves ◽  
Extraction And Purification ◽  
Immature Rats ◽  
Human Menopausal Gonadotrophin ◽  
Reference Preparation ◽  
The One

ABSTRACT The gonadotrophic activities of the International Reference Preparation for Human Menopausal Gonadotrophin (HMG 24) and of its predecessor, the unofficial reference preparation (HMG 20A), were assayed against 7 other preparations of postmenopausal urines extracted and purified by different methods. The end-points of assay were the dose-response curves for mean uterine and ovarian weights in immature rats. A marked qualitative difference was found between the 7 different extracts on the one hand and the reference preparations on the other. This difference is tentatively assumed to be caused by a relatively greater loss of follicle stimulating hormone than of luteinizing hormone in the procedures used for the extraction and purification of HMG 24 and HMG 20A. It is concluded that HMG 24 and HMG 20A are unsuitable as reference preparations for human menopausal gonadotrophin and for hypophyseal gonadotrophins in general.

Dose-response Curves in the Fibrin Plate Assay Fibrinolytic Activity of Proteases

1974 ◽  
Vol 32 (02/03) ◽  
pp. 356-365 ◽  
Author(s):  
F Haverkate ◽  
D. W Traas
Keyword(s):  
Dose Response ◽  
Fibrinolytic Activity ◽  
Enzyme Concentration ◽  
Response Curves ◽  
Agar Gel ◽  
Porcine Tissue ◽  
Plate Assay ◽  
Fibrin Plate ◽  
Different Types ◽  
Dose Response Curves

SummaryIn the fibrin plate assay different types of relationships between the dose of applied proteolytic enzyme and the response have been previously reported. This study was undertaken to determine whether a generally valid relationship might exist.Trypsin, chymotrypsin, papain, the plasminogen activator urokinase and all of the microbial proteases investigated, including brinase gave a linear relationship between the logarithm of the enzyme concentration and the diameter of the circular lysed zone. A similar linearity of dose-response curves has frequently been found by investigators who used enzyme plate assays with substrates different from fibrin incorporated in an agar gel. Consequently, it seems that this linearity of dose-response curves is generally valid for the fibrin plate assay as well as for other enzyme plate bioassays.Both human plasmin and porcine tissue activator of plasminogen showed deviations from linearity of semi-logarithmic dose-response curves in the fibrin plate assay.

BIOASSAY OF THYROID HORMONE USING HYPOTHYROID TADPOLES

1962 ◽  
Vol 41 (1) ◽  
pp. 143-153 ◽  
Author(s):  
U. Henriques
Keyword(s):  
Thyroid Hormone ◽  
Dose Response ◽  
Developmental Rate ◽  
Response Curves ◽  
Sodium Salts ◽  
Dose Response Curves

ABSTRACT A bioassay of thyroid hormone has been developed using Xenopus larvae made hypothyroid by the administration of thiourea. Only tadpoles of uniform developmental rate were used. Thiourea was given just before the metamorphotic climax in concentrations that produced neoteni in an early metamorphotic stage. During maintained thiourea neotoni, 1-thyroxine and 1-triiodothyronine were added as sodium salts to the water for three days and at the end of one week the stage of metamorphosis produced was determined. In this way identical dose-response curves were obtained for the two compounds. No qualitative differences between their effects were noted except that triiodothyronine seemed more toxic than thyroxine in equivalent doses. Triiodothyronine was found to be 7–12 times as active as thyroxine.

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