scholarly journals Studies on rat ovarian receptors for lutropin (luteinizing hormone). Applicability of radioimmunoassay to measure lutropin bound to receptors

1976 ◽  
Vol 160 (3) ◽  
pp. 603-606 ◽  
Author(s):  
K Muralidhar ◽  
N R Moudgal
Keyword(s):  
Luteinizing Hormone ◽  
Dose Response ◽  
Response Curves ◽  
Dose Response Curves

Measurement of receptor-bound unlabelled physiologically active lutropin (luteinizing hormone, LH) was possible by a modified radioimmunoassay. The conventional radioimmunoassay conducted at 4° C was inadequate, whereas the modified assay performed at 37° C could measure receptor-bound lutropin. The radioimmunoassay at 37° C takes only 36h for completion compared with 5-7 days at 4° C. The sensitivity and range of dose-response curves are, however, unaltered. The validity of the technique was established by a number of criteria.

FURTHER OBSERVATIONS ON THE OVARIAN ASCORBIC ACID DEPLETION TEST FOR LUTEINIZING HORMONE

1965 ◽  
Vol 32 (1) ◽  
pp. 1-7 ◽  
Author(s):  
E. T. BELL ◽  
J. A. LORAINE ◽  
S. MUKERJI ◽  
PACHARA VISUTAKUL
Keyword(s):  
Ascorbic Acid ◽  
Luteinizing Hormone ◽  
Dose Response ◽  
Intravenous Route ◽  
Response Curves ◽  
Standard Preparation ◽  
Routes Of Administration ◽  
Seasonal Factors ◽  
Dose Response Curves ◽  
The Absolute

SUMMARY A modification of the ovarian ascorbic acid depletion (OAAD) method for luteinizing hormone (LH) is described in which the standard and test materials are administered by the intraperitoneal rather than by the intravenous route. The potency of NIH-LH when administered i.p. and i.v. was the same and the slopes of the log dose-response curves for the two routes of administration were very similar. Studies on the effect of seasonal factors on the OAAD method showed that both the slopes of the log dose-response curves and the absolute levels of ascorbic acid varied from one assay to another. The necessity for using a standard preparation in all assays of LH activity by the OAAD method is emphasized.

STUDIES ON THE DOSE-RESPONSE CURVES IN THE VENTRAL PROSTATE METHOD FOR LUTEINIZING HORMONE

1967 ◽  
Vol 56 (4) ◽  
pp. 608-618 ◽  
Author(s):  
Peter Christiansen
Keyword(s):  
Luteinizing Hormone ◽  
Dose Response ◽  
Human Chorionic Gonadotrophin ◽  
Ventral Prostate ◽  
Human Origin ◽  
Response Curves ◽  
Human Pituitary ◽  
Human Menopausal Gonadotrophin ◽  
Dose Response Curves ◽  
The One

ABSTRACT Six luteinizing hormone preparations of human, ovine and equine origin were assayed by a modification of Greep's ventral prostate method. The dose-response curves for human and equine preparations on the one hand and the ovine preparation on the other had a widely different course. The ovine preparation gave a flat curve and did not even in large dosis, exhibit prostatic weights of the magnitude obtained by human preparations. The human and equine preparations gave steep curves, equally steep for human chorionic gonadotrophin (HCG), human menopausal gonadotrophin (HMG), human pituitary gonadotrophin (HPG), and the equine preparation. The slope of the curve is unaffected by the route of administration of the hormones, subcutaneous or intraperitoneal. The causes of the differences in the course of the curves are discussed and the importance of using standards of human origin when testing hormonal substances from humans is pointed out.

Dose-response Curves in the Fibrin Plate Assay Fibrinolytic Activity of Proteases

1974 ◽  
Vol 32 (02/03) ◽  
pp. 356-365 ◽  
Author(s):  
F Haverkate ◽  
D. W Traas
Keyword(s):  
Dose Response ◽  
Fibrinolytic Activity ◽  
Enzyme Concentration ◽  
Response Curves ◽  
Agar Gel ◽  
Porcine Tissue ◽  
Plate Assay ◽  
Fibrin Plate ◽  
Different Types ◽  
Dose Response Curves

SummaryIn the fibrin plate assay different types of relationships between the dose of applied proteolytic enzyme and the response have been previously reported. This study was undertaken to determine whether a generally valid relationship might exist.Trypsin, chymotrypsin, papain, the plasminogen activator urokinase and all of the microbial proteases investigated, including brinase gave a linear relationship between the logarithm of the enzyme concentration and the diameter of the circular lysed zone. A similar linearity of dose-response curves has frequently been found by investigators who used enzyme plate assays with substrates different from fibrin incorporated in an agar gel. Consequently, it seems that this linearity of dose-response curves is generally valid for the fibrin plate assay as well as for other enzyme plate bioassays.Both human plasmin and porcine tissue activator of plasminogen showed deviations from linearity of semi-logarithmic dose-response curves in the fibrin plate assay.

OVARIAN ASCORBIC ACID DEPLETION TEST FOR LUTEINIZING HORMONE

1967 ◽  
Vol 56 (4) ◽  
pp. 619-625 ◽  
Author(s):  
Hans Jacob Koed ◽  
Christian Hamburger
Keyword(s):  
Ascorbic Acid ◽  
Luteinizing Hormone ◽  
Dose Response ◽  
Response Curve ◽  
Dose Response Curve ◽  
Human Origin ◽  
Response Curves ◽  
Significant Difference ◽  
The Mean ◽  
Different Levels

ABSTRACT Comparison of the dose-response curves for LH of ovine origin (NIH-LH-S8) and of human origin (IRP-HMG-2) using the OAAD test showed a small, though statistically significant difference, the dose-response curve for LH of human origin being a little flatter. Two standard curves for ovine LH obtained with 14 months' interval, were parallel but at different levels of ovarian ascorbic acid. When the mean ascorbic acid depletions were calculated as percentages of the control levels, the two curves for NIH-LH-S8 were identical. The use of standards of human origin in the OAAD test for LH activity of human preparations is recommended.

BIOASSAY OF THYROID HORMONE USING HYPOTHYROID TADPOLES

1962 ◽  
Vol 41 (1) ◽  
pp. 143-153 ◽  
Author(s):  
U. Henriques
Keyword(s):  
Thyroid Hormone ◽  
Dose Response ◽  
Developmental Rate ◽  
Response Curves ◽  
Sodium Salts ◽  
Dose Response Curves

ABSTRACT A bioassay of thyroid hormone has been developed using Xenopus larvae made hypothyroid by the administration of thiourea. Only tadpoles of uniform developmental rate were used. Thiourea was given just before the metamorphotic climax in concentrations that produced neoteni in an early metamorphotic stage. During maintained thiourea neotoni, 1-thyroxine and 1-triiodothyronine were added as sodium salts to the water for three days and at the end of one week the stage of metamorphosis produced was determined. In this way identical dose-response curves were obtained for the two compounds. No qualitative differences between their effects were noted except that triiodothyronine seemed more toxic than thyroxine in equivalent doses. Triiodothyronine was found to be 7–12 times as active as thyroxine.

Dose Response Curves for Microwave Ablation in the Cardioplegia-Arrested Porcine Heart

2005 ◽  
Vol 8 (4) ◽  
pp. E269-E274 ◽  
Author(s):  
Sydney L. Gaynor ◽  
Gregory D. Byrd ◽  
Michael D. Diodato ◽  
Yosuke Ishii ◽  
Anson M. Lee ◽  
...  
Keyword(s):  
Dose Response ◽  
Microwave Ablation ◽  
Response Curves ◽  
Porcine Heart ◽  
Dose Response Curves

Modeling Nonmonotonic Dose-Response Curves

2001 ◽  
Author(s):  
Quinton J. Nottingham ◽  
Jeffrey B. Birch ◽  
Barry A. Bodt
Keyword(s):  
Dose Response ◽  
Response Curves ◽  
Dose Response Curves

scholarly journals Comment Concerning the Effects of Light Intensity on Melatonin Suppression in the Review “Light Modulation of Human Clocks, Wake, and Sleep” by A. Prayag et al.

2021 ◽  
Vol 3 (1) ◽  
pp. 181-188
Author(s):  
Peter Bracke ◽  
Eowyn Van de Putte ◽  
Wouter R. Ryckaert
Keyword(s):  
Phase Shift ◽  
Light Intensity ◽  
Dose Response ◽  
Light Modulation ◽  
Response Curves ◽  
Practical Applications ◽  
Circadian Phase ◽  
Melatonin Suppression ◽  
Dose Response Curves ◽  
Circadian Phase Shift

Dose-response curves for circadian phase shift and melatonin suppression in relation to white or monochromatic nighttime illumination can be scaled to melanopic weighed illumination for normally constricted pupils, which makes them easier to interpret and compare. This is helpful for a practical applications.

Sign in / Sign up

Export Citation Format

Share Document