THE BINDING OF 131I-TRIIODOTHYRONINE BY SERUM PROTEINS AS AN IN VITRO TEST OF THYROID FUNCTION

1964 ◽  
Vol 46 (2) ◽  
pp. 161-169 ◽  
Author(s):  
A. Cuarón ◽  
M. E. Fucugauchi
Keyword(s):  
Thyroid Function ◽  
Screening Test ◽  
Serum Proteins ◽  
In Vitro Test ◽  
Radiation Hazards ◽  
Clinical Screening ◽  
Sephadex Column ◽  
Vitro Test ◽  
High Degree

ABSTRACT The efficiency of Sephadex column chromatography for the separation of free iodide, free T3, and protein bound T3 has been demonstrated. Protein bound T3 and free iodide appear as two well defined peaks of activity in the eluate, while free T3 remains bound to the Sephadex column. This methods makes the direct study of the binding of T3 to serum proteins possible and allows of correction for the free radioactive iodide present as a contaminant. The value of this method in 147 selected patients was demonstrated by the high degree of accuracy in the diagnosis of thyroid function. The clinical diagnosis was confirmed by this method in 141 patients (95.9%) and the test failed in 6 cases (4.1%). The use of this method as a clinical screening test of thyroid function is suggested as it is sufficiently reliable, simple to perform and does not expose the patient to radiation hazards.

THE UPTAKE OF 131I TRIIODOTHYRONINE BY RESIN. AN IN VITRO TEST OF THYROID FUNCTION

1961 ◽  
Vol 37 (4) ◽  
pp. 607-615 ◽  
Author(s):  
M. G. Woldring ◽  
A. Bakker ◽  
H. Doorenbos
Keyword(s):  
Thyroid Function ◽  
Plasma Sample ◽  
In Vitro Test ◽  
Red Cell ◽  
Normal Range ◽  
Cell Substrate ◽  
Vitro Test ◽  
Uptake Test ◽  
Hyperthyroid Patients

ABSTRACT A technique is described, which resembles the in vitro red cell triiodothyronine uptake test for the investigation of thyroid function, but which requires only 0.5 ml of plasma. The uptake of radio-triiodothyronine is measured by a method in which resin replaces the erythrocytes. Thus the variable of the red cell substrate is excluded. By means of the resin technique described, an uptake of 14–27 % was found in a group of 130 euthyroid subjects. Fourteen plasma samples from hyperthyroid patients were investigated, which showed an uptake range of 25–40 % (one plasma sample from this group had an uptake of 25.8, i. e. within the normal range). In one case of hypothyroidism, an uptake of 10.7 % was found. It is not yet possible to state the level of uptake below which the diagnosis of hypothyroidism should be considered. The effect of different factors like the quantity of resin, incubationtemperature and incubation-time, pH and others have been investigated. The results are discussed.

Modification and evaluation of the chicken embryotoxicity screening test (CHEST) as in vitro test system for embryotoxicity

2009 ◽  
Vol 189 ◽  
pp. S82 ◽  
Author(s):  
Susanne Boehn ◽  
Hennicke Kamp ◽  
Tanja Kaufmann ◽  
Markus Koch ◽  
Petra Wessa ◽  
...  
Keyword(s):  
Screening Test ◽  
Test System ◽  
In Vitro Test ◽  
Vitro Test

scholarly journals Res-O-Mat T3 as an in vitro Test of Thyroid Function

RADIOISOTOPES ◽  
1970 ◽  
Vol 19 (3) ◽  
pp. 136-141
Author(s):  
Hirofumi MORI ◽  
Tsutomu MISHIMA ◽  
Kin-ichi HISADA
Keyword(s):  
Thyroid Function ◽  
In Vitro Test ◽  
Vitro Test

Lack of Influence of Povidone-Iodine on Tests of Thyroid Function

1978 ◽  
Vol 6 (5) ◽  
pp. 406-408 ◽  
Author(s):  
D P Lividas ◽  
G D Piperingos ◽  
J Sfontouris ◽  
D A Koutras
Keyword(s):  
Thyroid Function ◽  
Povidone Iodine ◽  
Test Methods ◽  
Thyroid Function Tests ◽  
In Vitro Test ◽  
External Application ◽  
Vitro Test ◽  
Antiseptic Agent

The external application of povidone-iodine, an antiseptic agent, was tested for its influence on thyroid function. Previous workers have described some in vitro changes in thyroid function tests following its use. In the present study topical application of povidone-iodine did not affect thyroid function as measured some days later using both in vivo and radio-active iodine in vitro test methods, despite the fact that the latter are notorious for being influenced by exogenous iodine.

The charcoal T3 ratio. An in vitro test of thyroid function

JAMA ◽  
1967 ◽  
Vol 199 (7) ◽  
pp. 469-472 ◽  
Author(s):  
L. E. Braverman
Keyword(s):  
Thyroid Function ◽  
In Vitro Test ◽  
Vitro Test

Filled and peptide-modified single-walled carbon nanotubes: synthesis, characterization, and in vitro test for cancer cell targeting

RSC Advances ◽  
2015 ◽  
Vol 5 (22) ◽  
pp. 16792-16800 ◽  
Author(s):  
Zhiyuan Hu ◽  
Jingjing Zhao ◽  
Zhaozheng Song ◽  
Chunpeng Yang
Keyword(s):  
Carbon Nanotubes ◽  
Cancer Cell ◽  
Single Walled Carbon Nanotubes ◽  
In Vitro Test ◽  
Single Walled Carbon ◽  
Vitro Test ◽  
Cancer Cell Targeting ◽  
High Degree ◽  
Walled Carbon Nanotubes

Multi-functional single-walled carbon nanotubes (SWNTs) with metal endohedral filling and a high degree of polycarboxylation on the sidewalls were synthesized without affecting the SWNTσ-framework.

Haemostatic Platelet Plug Formation in the Isolated Rabbit Mesenteric Preparation - An Analysis of Red Blood Cell Participation

1980 ◽  
Vol 44 (01) ◽  
pp. 006-008 ◽  
Author(s):  
D Bergqvist ◽  
K-E Arfors
Keyword(s):  
Whole Blood ◽  
Platelet Rich Plasma ◽  
Adenosine Diphosphate ◽  
In Vitro Test ◽  
Pharmacological Agents ◽  
Vitro Test ◽  
Releasing Effect ◽  
Plug Formation

SummaryIn a model using an isolated rabbit mesenteric preparation microvessels were transected and the time until haemostatic plugs formed was registered. Perfusion of platelet rich plasma gave no haemostasis whereas whole blood did. Addition of chlorpromazine or adenosine to the whole blood significantly prolonged the time for haemostasis, and addition of ADP to the platelet rich plasma significantly shortened it. It is concluded that red cells are necessary for a normal haemostasis in this model, probably by a combination of a haemodynamic and ADP releasing effect.The fundamental role of platelets in haemostatic plug formation is unquestionable but there are still problems concerning the stimulus for this process to start. Three platelet aggregating substances have been discussed – thrombin, adenosine diphosphate (ADP) and collagen. Evidence speaking in favour of thrombin is, however, very minimal, and the discussion has to be focused on collagen and ADP. In an in vitro system using polyethylene tubings we have shown that "haemostasis" can be obtained without the presence of collagen but against these results can be argued that it is only another in vitro test for platelet aggregation (1).To be able to induce haemostasis in this model, however, the presence of red blood cells is necessary. To further study this problem we have developed a model where haemostatic plug formation can be studied in the isolated rabbit mesentery and we have briefly reported on this (2).Thus, it is possible to perfuse the vessels with whole blood as well as with platelet rich plasma (PRP) and different pharmacological agents of importance.

scholarly journals In Vitro Newly Isolated Environmental Phage Activity against Biofilms Preformed by Pseudomonas aeruginosa from Patients with Cystic Fibrosis

2021 ◽  
Vol 9 (3) ◽  
pp. 478
Author(s):  
Ersilia Vita Fiscarelli ◽  
Martina Rossitto ◽  
Paola Rosati ◽  
Nour Essa ◽  
Valentina Crocetta ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Pseudomonas Aeruginosa ◽  
Multidrug Resistant ◽  
In Vitro Test ◽  
Chronic Infections ◽  
Hospital Environments ◽  
Vitro Test ◽  
General Reliability ◽  
Phage Activity

As disease worsens in patients with cystic fibrosis (CF), Pseudomonas aeruginosa (PA) colonizes the lungs, causing pulmonary failure and mortality. Progressively, PA forms typical biofilms, and antibiotic treatments determine multidrug-resistant (MDR) PA strains. To advance new therapies against MDR PA, research has reappraised bacteriophages (phages), viruses naturally infecting bacteria. Because few in vitro studies have tested phages on CF PA biofilms, general reliability remains unclear. This study aimed to test in vitro newly isolated environmental phage activity against PA isolates from patients with CF at Bambino Gesù Children’s Hospital (OBG), Rome, Italy. After testing in vitro phage activities, we combined phages with amikacin, meropenem, and tobramycin against CF PA pre-formed biofilms. We also investigated new emerging morphotypes and bacterial regrowth. We obtained 22 newly isolated phages from various environments, including OBG. In about 94% of 32 CF PA isolates tested, these phages showed in vitro PA lysis. Despite poor efficacy against chronic CF PA, five selected-lytic-phages (Φ4_ZP1, Φ9_ZP2, Φ14_OBG, Φ17_OBG, and Φ19_OBG) showed wide host activity. The Φ4_ZP1-meropenem and Φ14_OBG-tobramycin combinations significantly reduced CF PA biofilms (p < 0.001). To advance potential combined phage-antibiotic therapy, we envisage further in vitro test combinations with newly isolated phages, including those from hospital environments, against CF PA biofilms from early and chronic infections.

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