INDIVIDUAL DETERMINATION OF CORTISOL AND CORTICOSTERONE IN A SINGLE SMALL SAMPLE OF PERIPHERAL BLOOD

1961 ◽  
Vol 38 (3) ◽  
pp. 399-406 ◽  
Author(s):  
J. van der Vies
Keyword(s):  
Sulfuric Acid ◽  
Carbon Tetrachloride ◽  
Peripheral Blood ◽  
Partition Coefficients ◽  
Small Sample ◽  
Peripheral Plasma ◽  
Individual Determination ◽  
The Individual

ABSTRACT A method is described for the individual determination of corticosterone (11β,21-dihydroxy-pregn-4-ene-3,20-dione) and cortisol (11β,17,21 -trihydroxy-pregn-4-ene-3,20-dione) in 2.5 ml of peripheral plasma. The method is based on the different partition coefficients of these steroids between water and carbon tetrachloride, allowing of separation by simple extractions. The steroids are determined by reading the fluorescence in a mixture of sulfuric acid and alcohol.

SOME CLINICAL EXPERIENCE IN THE DETERMINATION OF CORTISOL AND CORTICOSTERONE IN A SMALL SAMPLE OF HUMAN PLASMA

1961 ◽  
Vol 38 (3) ◽  
pp. 392-398 ◽  
Author(s):  
B. van der Wal ◽  
A. L. M. Israëls ◽  
J. F. Janssen ◽  
D. de Wied
Keyword(s):  
Rheumatoid Arthritis ◽  
Carbon Tetrachloride ◽  
Human Plasma ◽  
Plasma Cortisol ◽  
Partition Coefficients ◽  
Corticosterone Level ◽  
Plasma Corticosterone ◽  
Small Sample ◽  
Adults And Children

ABSTRACT Quantitative determinations of cortisol and corticosterone were performed in 3 ml of human plasma, using the different partition coefficients of these steroids between water and carbon tetrachloride according to Van der Vies (1961). Comparative values for plasma cortisol and corticosterone in adults and children and in patients treated with corticotrophin (ACTH) or corticosteroids are presented. Cortisol levels in non-treated adults and in children amounted to 11.8 ± 2.0 ranging from 5–26 μg per 100 ml while mean corticosterone levels were found to be 2.75 ± 0.70 ranging from 0–6 μg per 100 ml. Intravenous Cortrophin or Pitressin for one hour or intramuscular Cortrophin Z generally increased both plasma cortisol and corticosterone, whereas treatment with prednisone somewhat decreased the cortisol content of plasma without affecting the plasma corticosterone. One patient with Cushing's syndrome had a high cortisol and a normal corticosterone level and in an adult patient with rheumatoid arthritis treated for several months with Cortrophin Z, the rather high cortisol level was associated with a very low corticosterone content of the plasma.

Collaborative Study Using a ZDBT Colorimetric Method for the Determination of Copper in Alcoholic Products

1967 ◽  
Vol 50 (2) ◽  
pp. 334-338
Author(s):  
Duane H Strunk ◽  
A A Andreasen
Keyword(s):  
Sulfuric Acid ◽  
Carbon Tetrachloride ◽  
Collaborative Study ◽  
Colorimetric Method ◽  
Good Precision ◽  
Determination Of Copper

Abstract Results are given on a collaborative study in which a zinc dibenzyldithiocarbamate (ZDBT) colorimetric method is used to measure copper in alcoholic products such as high wine, spirits, gin, whisky, brandy, rum, and wine. In this method, the sample is made ca 0.SN with sulfuric acid, and carbon tetrachloride containing 0.2% ZDBT is added. The colored copper-ZDBT complex is extracted in the carbon tetrachloride and measured at 438 mμ against a similar carbon tetrachloride extract of a blank. Data show good precision, and it is recommended that the ZDBT method be adopted as official, first action.

Liquid Chromatographic Determination of Cinnamic and Benzoic Acids in Benzoin Preparations

1987 ◽  
Vol 70 (4) ◽  
pp. 689-691
Author(s):  
Abdel-Aziz M Wahbi ◽  
Mohammad A Abounassif ◽  
El-Rasheed A Gad-Kariem ◽  
Mahmoud W Ibrahim
Keyword(s):  
Chromatographic Determination ◽  
Cinnamic Acids ◽  
Reverse Phase ◽  
British Pharmacopoeia ◽  
Liquid Chromatographic Method ◽  
Individual Determination ◽  
Liquid Chromatographic Determination ◽  
The Individual ◽  
Liquid Chromatographic

Abstract A liquid chromatographic method for the individual determination of benzoic and cinnamic acids in 2 benzoin preparations is presented. The method specifies a reverse phase column and 0.01M KH2P04- methanol (85 + 15) as mobile phase at a flow rate of 1.8 mL/min, with detection at 254 nm. The method has been applied to 2 benzoin preparations and the results were compared with those from the British Pharmacopoeia method.

Toxicologic Studies on Hydrocarbons. VI. A Colorimetric Method for the Determination of Kerosine in Blood

1960 ◽  
Vol 6 (4) ◽  
pp. 327-331 ◽  
Author(s):  
Horace W Gerarde ◽  
Paul Skiba
Keyword(s):  
Sulfuric Acid ◽  
Carbon Tetrachloride ◽  
Quantitative Determination ◽  
Calibration Curve ◽  
Colorimetric Method ◽  
Acid Reagent

Abstract A photoelectric colorimetric method is described for the quantitative determination of kerosine in blood. The procedure involves hemolysis of 5 ml. of the sample followed by extraction of the kerosine with carbon tetrachloride. The extract is reacted with a formaldehyde- sulfuric acid reagent to produce a characteristic color. The intensity of this color is measured photometrically, and the concentration of kerosine is determined by reference to a previously prepared calibration curve. Concentrations as low as 10 ppm can be conveniently determined.

scholarly journals Simultaneous Enantiomeric Separation of Carfentrazone-Ethyl Herbicide and Its Hydrolysis Metabolite Carfentrazone by Cyclodextrin Electrokinetic Chromatography. Analysis of Agrochemical Products and a Degradation Study

Molecules ◽  
2021 ◽  
Vol 26 (17) ◽  
pp. 5350
Author(s):  
Laura García-Cansino ◽  
María Ángeles García ◽  
María Luisa Marina
Keyword(s):  
Enantiomeric Separation ◽  
Chiral Selector ◽  
Electrokinetic Chromatography ◽  
Reverse Polarity ◽  
Degradation Study ◽  
Chromatography Analysis ◽  
Simultaneous Separation ◽  
Individual Determination ◽  
The Individual

The different activity and toxicity that the enantiomers of agrochemicals may have requires the development of stereoselective analytical methodologies enabling the individual determination of each enantiomer. The aim of this work was to develop the first Electrokinetic Chromatography methodology enabling the simultaneous enantiomeric separation of carfentrazone-ethyl herbicide and its hydrolysis metabolite carfentrazone. The use of an anionic cyclodextrin as chiral selector (captisol at 2.5% (w/v)) in a 25 mM acetate buffer, at a temperature of 30 °C, and an applied voltage (reverse polarity) of −30 kV, allowed the simultaneous separation of the four enantiomers of the two compounds studied in 6.8 min with enantiomeric resolutions of 5.0 for carfentrazone-ethyl and 5.1 for carfentrazone. Analytical characteristics of the developed method were evaluated and found adequate to achieve the quantitation of carfentrazone-ethyl and carfentrazone. Analysis of a commercial herbicide formulation showed the potential of the method for the quality control of these agrochemical products. Degradation studies for carfentrazone-ethyl revealed that no significant degradation took place in cleaned sand samples while a significant but not stereoselective degradation took place in soils for the whole period of time considered (seven days).

EUTANÁSIA E O AUXÍLIO AO SUICÍDIO: UM ESTUDO SOBRE A DESCRIMINALIZAÇÃO DO HOMICÍDIO PIEDOSO

2021 ◽  
Vol 9 (208) ◽  
pp. 1-17
Author(s):  
Rodolfo de Holanda Freitas
Keyword(s):  
Social Context ◽  
Legal System ◽  
Religion And Medicine ◽  
Different Types ◽  
Individual Determination ◽  
The Subject ◽  
The Individual ◽  
The Right ◽  
Historical Scenario

This article will carry out an analysis on the practice of Euthanasia, going through its etymology, historical scenario, principles involved, as well as its understanding in our legal system and social context. In addition, an analysis of a real case in Brazil and a comparison with other countries where the practice is legal is made. This article is mainly aimed at minimally elucidating the controversy surrounding the topic, which is still very stigmatized in our country, since it involves several principles not only of law, but of religion and medicine, making a comparison between them. For the realization of this article, bibliographic research had been carried out through articles, legal sites and news sites, using deductive methodology for its understanding and outcome. There is a great taboo on the subject in our country, given that it can have different types of understanding according to the individual determination of each one, with much debate still remaining so that, finally, sick patients have the right to put their lives the best way possible.

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