scholarly journals Histological Evaluation of Experimentally Induced Critical Size Defect Skin Wounds Using Exosomal Solution of Mesenchymal Stem Cells Derived Microvesicles

2017 ◽  
Vol 10 (2) ◽  
pp. 144-153 ◽  
Author(s):  
Omar S. El-Tookhy ◽  
Ashraf A. Shamaa ◽  
Gehan G. Shehab ◽  
Ahmed N. Abdallah ◽  
Omnia M. Azzam
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Critical Size ◽  
Histological Evaluation ◽  
Skin Wounds ◽  
Critical Size Defect ◽  
Experimentally Induced

Xenogenic Transplantation of Human Mesenchymal Stem Cells in a Critical Size Defect of the Sheep Tibia for Bone Regeneration

2010 ◽  
Vol 16 (1) ◽  
pp. 33-43 ◽  
Author(s):  
Philipp Niemeyer ◽  
Thomas S. Schönberger ◽  
Joachim Hahn ◽  
Philip Kasten ◽  
Joerg Fellenberg ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Regeneration ◽  
Critical Size ◽  
Human Mesenchymal Stem Cells ◽  
Critical Size Defect

scholarly journals Improved healing of critical-size femoral defect in osteoporosis rat models using 3D elastin/polycaprolactone/nHA scaffold in combination with mesenchymal stem cells

2021 ◽  
Vol 32 (3) ◽  
Author(s):  
Fatemeh Hejazi ◽  
Vahid Ebrahimi ◽  
Mehrdad Asgary ◽  
Abbas Piryaei ◽  
Mohammad Javad Fridoni ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Healing ◽  
Critical Size ◽  
Therapeutic Potential ◽  
Rna Extraction ◽  
Osteotomy Site ◽  
Cell Groups ◽  
The Right

AbstractOsteoporosis is a common bone disease that results in elevated risk of fracture, and delayed bone healing and impaired bone regeneration are implicated by this disease. In this study, Elastin/Polycaprolactone/nHA nanofibrous scaffold in combination with mesenchymal stem cells were used to regenerate bone defects. Cytotoxicity, cytocompatibility and cellular morphology were evaluated in vitro and observations revealed that an appropriate environment for cellular attachment, growth, migration, and proliferation is provided by this scaffold. At 3 months following ovariectomy (OVX), the rats were used as animal models with an induced critical size defect in the femur to evaluate the therapeutic potential of osteogenic differentiation of bone marrow mesenchymal stem cells (BM-MSCs) seeded on 3 dimension (3D) scaffolds. In this experimental study, 24 female Wistar rats were equally divided into three groups: Control, scaffold (non-seeded BM-MSC), and scaffold + cell (seeded BM-MSC) groups. 30 days after surgery, the right femur was removed, and underwent a stereological analysis and RNA extraction in order to examine the expression of Bmp-2 and Vegf genes. The results showed a significant increase in stereological parameters and expression of Bmp-2 and Vegf in scaffold and scaffold + cell groups compared to the control rats. The present study suggests that the use of the 3D Elastin/Polycaprolactone (PCL)/Nano hydroxyapatite (nHA) scaffold in combination with MSCs may improve the fracture regeneration and accelerates bone healing at the osteotomy site in rats.

Influence of a titanium mesh on the management of segmental long bone defects

2015 ◽  
Vol 28 (06) ◽  
pp. 417-424 ◽  
Author(s):  
S. A. Papadimitriou ◽  
A. D. Galatos ◽  
N. N. Prassinos ◽  
D. Psalla ◽  
M. Dalstra ◽  
...  
Keyword(s):  
Critical Size ◽  
Experimental Studies ◽  
Long Bone ◽  
Histological Evaluation ◽  
Autogenous Bone ◽  
Bone Block ◽  
Titanium Mesh ◽  
Micro Computed Tomography ◽  
Critical Size Defect ◽  
Group B

SummaryObjectives: To evaluate the influence of titanium mesh on guided bone regeneration when used, either alone or in combination with autogenous bone block graft, in a canine ulnar model.Methods: Thirty-two, purpose bred, adult, castrated male Beagles were used, divided into four equal-size groups. A unilateral middiaphyseal ulnar critical-size defect was created in each dog. The ulnar segments were stabilized with a stainless-steel plate and screws. Each defect was managed by: no further treatment (Group A) or by placement of a bone block graft taken from the ipsilateral iliac crest (Group B), or titanium mesh wrapped around the ulna (Group C), or a bone block graft and titanium mesh (Group D). After six months, bone block biopsies were performed and the samples were scanned using micro-computed tomography. Qualitative histological evaluation was performed on two non-decalcified longitudinal sections from each block.Results: No significant differences in terms of mineralized bone volume were detected between the grafted sites (Groups B and D) or between the non-grafted ones (Groups A and C). The histological evaluation indicated good integration of the bone blocks irrespective of the use of titanium mesh.Clinical significance: The use of titanium mesh does not influence the amount of bone formation. The canine ulnar critical-size defect model seems to be a reliable model to use in experimental studies.

scholarly journals The Possible Role of Mesenchymal Stem Cells Therapy in the Repair of Experimentally Induced Colitis in Male Albino Rats

2013 ◽  
Vol 6 (2) ◽  
pp. 92-103 ◽  
Author(s):  
Sohair Ahmed Fawzy ◽  
Rahma Kamal El-din Abo-Elnou ◽  
Dalia Fathy Abd-El-Maksoud El-Deeb ◽  
Marwa Mohamed Yousry Abd-Elkader
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Albino Rats ◽  
Stem Cells Therapy ◽  
Experimentally Induced
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