scholarly journals Regeneration of Bovine Mammary Gland in Immunodeficient Mice by Transplantation of Bovine Mammary Epithelial Cells Mixed with Matrigel

2016 ◽  
Vol 9 (2) ◽  
pp. 186-191 ◽  
Author(s):  
Hyun Jung Park ◽  
Won Young Lee ◽  
Ha Yeon Jeong ◽  
Hyuk Song
Keyword(s):  
Mammary Gland ◽  
Epithelial Cells ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Bovine Mammary Gland ◽  
Bovine Mammary Epithelial Cells ◽  
Immunodeficient Mice

Bovine mammary epithelial cells, initiators of innate immune responses to mastitis

2005 ◽  
Vol 45 (8) ◽  
pp. 757 ◽  
Author(s):  
C. Gray ◽  
Y. Strandberg ◽  
L. Donaldson ◽  
R. L. Tellam
Keyword(s):  
Immune Response ◽  
Mammary Gland ◽  
Epithelial Cells ◽  
Mammary Epithelial Cells ◽  
Vital Role ◽  
Mammary Epithelial ◽  
Innate Immune ◽  
Mammary Tissue ◽  
Effector Cells ◽  
Bovine Mammary Epithelial Cells

Innate immunity plays a vital role in the protection of the bovine mammary gland against mastitis. Until recently, the migration of effector cells such as neutrophils and monocytes into the mammary gland was thought to provide the only defence against invading pathogens. However, mammary epithelial cells may also play an important role in the immune response, contributing to the innate defence of the mammary tissue through secretion of antimicrobial peptides and attraction of circulating immune effector cells. This paper reviews the innate immune pathways in mammary epithelial cells and examines their role in the initiation of an innate immune response to Gram-positive and Gram-negative bacteria.

scholarly journals Metabolomic Profiles of Bovine Mammary Epithelial Cells Stimulated by Lipopolysaccharide

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Yixin Huang ◽  
Liuhong Shen ◽  
Jing Jiang ◽  
Qipin Xu ◽  
Zhengzhong Luo ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Linoleic Acid ◽  
Epithelial Cells ◽  
High Performance ◽  
Acid Metabolism ◽  
Mammary Epithelial Cells ◽  
Phospholipid Metabolism ◽  
Mammary Epithelial ◽  
Multivariate Statistical ◽  
Bovine Mammary Epithelial Cells

AbstractBovine mammary epithelial cells (bMECs) are the main cells of the dairy cow mammary gland. In addition to their role in milk production, they are effector cells of mammary immunity. However, there is little information about changes in metabolites of bMECs when stimulated by lipopolysaccharide (LPS). This study describes a metabolomics analysis of the LPS-stimulated bMECs to provide a basis for the identification of potential diagnostic screening biomarkers and possible treatments for bovine mammary gland inflammation. In the present study, bMECs were challenged with 500 ng/mL LPS and samples were taken at 0 h, 12 h and 24 h post stimulation. Metabolic changes were investigated using high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-Q-TOF MS) with univariate and multivariate statistical analyses. Clustering and metabolic pathway changes were established by MetaboAnalyst. Sixty-three differential metabolites were identified, including glycerophosphocholine, glycerol-3-phosphate, L-carnitine, L-aspartate, glutathione, prostaglandin G2, α-linolenic acid and linoleic acid. They were mainly involved in eight pathways, including D-glutamine and D-glutamic acid metabolism; linoleic acid metabolism; α-linolenic metabolism; and phospholipid metabolism. The results suggest that bMECs are able to regulate pro-inflammatory, anti-inflammatory, antioxidation and energy-producing related metabolites through lipid, antioxidation and energy metabolism in response to inflammatory stimuli.

Gene expression and hormonal regulation of adiponectin and its receptors in bovine mammary gland and mammary epithelial cells

2010 ◽  
Vol 82 (1) ◽  
pp. 99-106 ◽  
Author(s):  
Yoshihisa OHTANI ◽  
Tomo YONEZAWA ◽  
Sang-Houn SONG ◽  
Tatsuyuki TAKAHASHI ◽  
Astrid ARDIYANTI ◽  
...  
Keyword(s):  
Gene Expression ◽  
Mammary Gland ◽  
Epithelial Cells ◽  
Hormonal Regulation ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Bovine Mammary Gland

scholarly journals Allograft inflammatory factor-1 (AIF-1) induces inflammatory mediator production via NF-κB signaling and impairs bovine mammary epithelial cells via mitochondrial pathway

2020 ◽  
Author(s):  
Danru Yang ◽  
Yinghuan Wu ◽  
Yanying Zhao
Keyword(s):  
Mammary Gland ◽  
Epithelial Cells ◽  
Cell Membrane ◽  
Cell Apoptosis ◽  
Mammary Epithelial Cells ◽  
Bovine Mastitis ◽  
Mammary Epithelial ◽  
Cell Membrane Permeability ◽  
Inflammatory Factor ◽  
Bovine Mammary Epithelial Cells

Abstract Background Bovine mastitis is the inflammatory response of the mammary gland with an utmost threat to the dairy industry worldwide. Cytokine networks fuel inflammation. The sensitive and subtle changes of the inflammatory cytokine network in healthy and mastitic bovine mammary gland may encourage the use of cytokines in the diagnosis and prognosis of bovine mastitis. Allograft inflammatory factor-1 (AIF-1) is a proinflammatory cytokine mainly secreted by immune cells and it plays a central role in the complex signaling network of inflammation activation. Therefore, we explored the possible role of bovine AIF-1 related to bovine mastitis in the present study. Results The average concentration of AIF-1 in milks suffering from mastitis was 2.5 fold of that in the healthy cows, while its value decreased in cows recovered from mastitis. Furthermore, recombinant bovine AIF-1 up-regulated TNF-α, IL-6, and monocyte chemoattractant protein 1 secretion from bovine mammary epithelial cells with NF-κB activating, then NF-κB signaling inhibitor BAY 11-7085 abolished the increase of these inflammatory cytokines secretion induced by AIF-1. Thereafter, AIF-1 impaired bovine mammary epithelial cell viability, induced cell membrane permeability and cell apoptosis with exacerbated nitric oxide and oxidative stress, activated caspase 3, decreased mitochondrial membrane potential and intracellular ATP concentration. Conclusion These results indicated that AIF-1 prompted inflammation mediator production of bovine mammary epithelial cells via NF-κB signaling. Moreover, it damaged epithelial cells by depressing cell viability, inducing cell membrane permeability and cell apoptosis, which might be related to bovine mastitis.

scholarly journals Staphylococcus aureus Phenol-Soluble Modulins Impair Interleukin Expression in Bovine Mammary Epithelial Cells

2016 ◽  
Vol 84 (6) ◽  
pp. 1682-1692 ◽  
Author(s):  
Martine Deplanche ◽  
Ludmila Alekseeva ◽  
Ksenia Semenovskaya ◽  
Chih-Lung Fu ◽  
Frederic Dessauge ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Mammary Gland ◽  
Epithelial Cells ◽  
Mammary Epithelial Cells ◽  
Dendritic Cell Maturation ◽  
Mammary Epithelial ◽  
Content Type ◽  
Bovine Mammary Epithelial Cells ◽  
E Coli ◽  
Infected Cells

The role of the recently described interleukin-32 (IL-32) inStaphylococcus aureus-induced mastitis, an inflammation of the mammary gland, is unclear. We determined expression of IL-32, IL-6, and IL-8 inS. aureus- andEscherichia coli-infected bovine mammary gland epithelial cells. Using live bacteria, we found that inS. aureus-infected cells, induction of IL-6 and IL-8 expression was less pronounced than inE. coli-infected cells. Notably, IL-32 expression was decreased inS. aureus-infected cells, while it was increased inE. coli-infected cells. We identified the staphylococcal phenol-soluble modulin (PSM) peptides as key contributors to these effects, as IL-32, IL-6, and IL-8 expression by epithelial cells exposed topsmmutant strains was significantly increased compared to that in cells exposed to the isogenicS. aureuswild-type strain, indicating that PSMs inhibit the production of these interleukins. The use of genetically complemented strains confirmed this observation. Inasmuch as the decreased expression of IL-32, which is involved in dendritic cell maturation, impairs immune responses, our results support a PSM-dependent mechanism that allows for the development of chronicS. aureus-related mastitis.

Lactobacillus casei BL23 modulates the innate immune response in Staphylococcus aureus-stimulated bovine mammary epithelial cells

2018 ◽  
Vol 9 (6) ◽  
pp. 985-995 ◽  
Author(s):  
R.F.S. Souza ◽  
L. Rault ◽  
N. Seyffert ◽  
V. Azevedo ◽  
Y. Le Loir ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Immune Response ◽  
Mammary Gland ◽  
Epithelial Cells ◽  
Lactobacillus Casei ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Innate Immune ◽  
Bovine Mammary Epithelial Cells ◽  
Aureus Infection

Probiotics have been adopted to treat and prevent various diseases in humans and animals. They were notably shown to be a promising alternative to prevent mastitis in dairy cattle. This inflammation of the mammary gland is generally of infectious origin and generates extensive economic losses worldwide. In a previous study, we found that Lactobacillus casei BL23 was able to inhibit the internalisation of Staphylococcus aureus, one of the major pathogens involved in mastitis, into bovine mammary epithelial cells (bMEC). In this study, we further explored the capacity of this strain to modulate the innate immune response of bovine mammary epithelial cells during S. aureus infection. L. casei BL23 was able to decrease the expression of several pro-inflammatory cytokines, including interleukins 6, 8, 1α and 1β and tumour necrosis factor alpha, in S. aureus-stimulated bMEC, 8 h post-infection. On the other hand, L. casei did not impair the induction of defensins, such as lingual antimicrobial peptide and defensin β1 in the presence of S. aureus, and even slightly increased the induction of tracheal antimicrobial peptide during S. aureus infection. Finally, this strain did not alter the expression of the pattern recognition receptor nucleotide-binding oligomerisation domain proteins (NOD2). This study demonstrates that L. casei BL23 displayed anti-inflammatory properties on S. aureus-stimulated bMEC. These results open the way to further characterisation of the BL23 probiotic potential in a bovine mammary gland context and to a better understanding of how all these beneficial properties combine in vivo to combat mastitis pathogens.

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