scholarly journals The transcription factor Cabut coordinates energy metabolism and the circadian clock in response to sugar sensing

2015 ◽  
Vol 34 (11) ◽  
pp. 1538-1553 ◽  
Author(s):  
Osnat Bartok ◽  
Mari Teesalu ◽  
Reut Ashwall‐Fluss ◽  
Varun Pandey ◽  
Mor Hanan ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Energy Metabolism ◽  
Circadian Clock ◽  
Sugar Sensing

scholarly journals Clock-associated genes in Arabidopsis : a family affair

2001 ◽  
Vol 356 (1415) ◽  
pp. 1745-1753 ◽  
Author(s):  
David E. Somers
Keyword(s):  
Transcription Factor ◽  
Circadian Clock ◽  
Gene Families ◽  
Forward Genetics ◽  
Pas Domain ◽  
Receiver Domain ◽  
Domain Structures ◽  
Two Component ◽  
Similarities And Differences ◽  
Signalling Systems

The identification of components of the plant circadian clock has been advanced recently with the success of two forward genetics approaches. The ZEITLUPE and TOC1 loci were cloned and each was found to be part of two separate, larger gene families with intriguing domain structures. The ZTL family of proteins contains a subclass of the PAS domain coupled to an F box and kelch motifs, suggesting that they play a role in a novel light–regulated ubiquitination mechanism. TOC1 shares similarity to the receiver domain of the well–known two–component phosphorelay signalling systems, combined with a strong similarity to a region of the CONSTANS transcription factor, which is involved in controlling flowering time. When added to the repertoire of previously identified clock–associated genes, it is clear that both similarities and differences with other circadian systems will emerge in the coming years.

scholarly journals Transcriptional Feedback of Neurospora Circadian Clock Gene by Phosphorylation-Dependent Inactivation of Its Transcription Factor

Cell ◽  
2005 ◽  
Vol 122 (2) ◽  
pp. 235-246 ◽  
Author(s):  
Tobias Schafmeier ◽  
Andrea Haase ◽  
Krisztina Káldi ◽  
Johanna Scholz ◽  
Marc Fuchs ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Circadian Clock ◽  
Clock Gene ◽  
Circadian Clock Gene ◽  
Dependent Inactivation ◽  
Transcriptional Feedback

scholarly journals Circadian clock and liver energy metabolism

2020 ◽  
Vol 28 (20) ◽  
pp. 1025-1035
Author(s):  
Wen-Kang Gao ◽  
Yan-Yun Shu ◽  
Jin Ye ◽  
Xiao-Li Pan
Keyword(s):  
Energy Metabolism ◽  
Circadian Clock

scholarly journals Core circadian clock transcription factor BMAL1 regulates mammary epithelial cell growth, differentiation, and milk component synthesis

2021 ◽  
Author(s):  
Theresa Casey ◽  
Aridany Suarez-Trujillo ◽  
Shelby Cummings ◽  
Katelyn Huff ◽  
Jennifer Crodian ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Circadian Clock ◽  
Mammary Epithelial Cells ◽  
Transcriptional Start Site ◽  
Prolactin Receptor ◽  
Mammary Development ◽  
Mammary Epithelial ◽  
Pcr Analysis ◽  
Protein Coding ◽  
Lower Expression

ABSTRACTThe role the mammary epithelial circadian clock plays in gland development and lactation is unknown. We hypothesized that mammary epithelial clocks function to regulate mammogenesis and lactogenesis, and propose the core clock transcription factor BMAL1:CLOCK regulates genes that control mammary epithelial development and milk synthesis. Our objective was to identify transcriptional targets of BMAL1 in undifferentiated (UNDIFF) and lactogen differentiated (DIFF) mammary epithelial cells (HC11) using ChIP-seq. Ensembl gene IDs with the nearest transcriptional start site to peaks were explored as potential targets, and represented 846 protein coding genes common to UNDIFF and DIFF cells and 2773 unique to DIFF samples. Genes with overlapping peaks between samples (1343) enriched cell-cell adhesion, membrane transporters and lipid metabolism categories. To functionally verify targets, an HC11 line with Bmal1 gene knocked out (BMAL1-KO) using CRISPR-CAS was created. BMAL1-KO cultures had lower cell densities over an eight-day growth curve, which was associated with increased (p<0.05) levels of reactive oxygen species and lower expression of superoxide dismutase 3 (Sod3). Q-PCR analysis also found lower expression of the putative targets, prolactin receptor (Prlr), Ppara, and beta-casein (Csn2). Findings support our hypothesis and highlight potential importance of clock in mammary development and substrate transport.

scholarly journals Physiologic Mechanical Stress Directly Induces Bone Formation by Activating Glucose Transporter 1 (Glut 1) in Osteoblasts, Inducing Signaling via NAD+-Dependent Deacetylase (Sirtuin 1) and Runt-Related Transcription Factor 2 (Runx2)

2021 ◽  
Vol 22 (16) ◽  
pp. 9070
Author(s):  
Shu Somemura ◽  
Takanori Kumai ◽  
Kanaka Yatabe ◽  
Chizuko Sasaki ◽  
Hiroto Fujiya ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Energy Metabolism ◽  
Mechanical Stress ◽  
Mechanical Loading ◽  
Glucose Transporter ◽  
Sirtuin 1 ◽  
Glucose Transporter 1 ◽  
Cellular Energy ◽  
Osteoblast Activity ◽  
Related Transcription Factor

Mechanical stress is an important factor affecting bone tissue homeostasis. We focused on the interactions among mechanical stress, glucose uptake via glucose transporter 1 (Glut1), and the cellular energy sensor sirtuin 1 (SIRT1) in osteoblast energy metabolism, since it has been recognized that SIRT1, an NAD+-dependent deacetylase, may function as a master regulator of the mechanical stress response as well as of cellular energy metabolism (glucose metabolism). In addition, it has already been demonstrated that SIRT1 regulates the activity of the osteogenic transcription factor runt-related transcription factor 2 (Runx2). The effects of mechanical loading on cellular activities and the expressions of Glut1, SIRT1, and Runx2 were evaluated in osteoblasts and chondrocytes in a 3D cell–collagen sponge construct. Compressive mechanical loading increased osteoblast activity. Mechanical loading also significantly increased the expression of Glut1, significantly decreased the expression of SIRT1, and significantly increased the expression of Runx2 in osteoblasts in comparison with non-loaded osteoblasts. Incubation with a Glut1 inhibitor blocked mechanical stress-induced changes in SIRT1 and Runx2 in osteoblasts. In contrast with osteoblasts, the expressions of Glut1, SIRT1, and Runx2 in chondrocytes were not affected by loading. Our present study indicated that mechanical stress induced the upregulation of Glut1 following the downregulation of SIRT1 and the upregulation of Runx2 in osteoblasts but not in chondrocytes. Since SIRT1 is known to negatively regulate Runx2 activity, a mechanical stress-induced downregulation of SIRT1 may lead to the upregulation of Runx2, resulting in osteoblast differentiation. Incubation with a Glut1 inhibitor the blocked mechanical stress-induced downregulation of SIRT1 following the upregulation of Runx2, suggesting that Glut1 is necessary to mediate the responses of SIRT1 and Runx2 to mechanical loading in osteoblasts.

scholarly journals Circadian Clock Regulation of Hepatic Energy Metabolism Regulatory Circuits

Biology ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 79
Author(s):  
Hunter ◽  
Ray
Keyword(s):  
Gene Expression ◽  
Energy Metabolism ◽  
Circadian Clock ◽  
Rhythmic Expression ◽  
Hepatic Energy Metabolism ◽  
Regulatory Circuits ◽  
Liver Transcriptome ◽  
Metabolic Gene Expression ◽  
Critical Organ ◽  
Hepatic Genes

The liver is a critical organ of energy metabolism. At least 10% of the liver transcriptome demonstrates rhythmic expression, implying that the circadian clock regulates large programmes of hepatic genes. Here, we review the mechanisms by which this rhythmic regulation is conferred, with a particular focus on the transcription factors whose actions combine to impart liver- and time-specificity to metabolic gene expression.

scholarly journals Circadian Network Interactions with Jasmonate Signaling and Defense

Plants ◽  
2019 ◽  
Vol 8 (8) ◽  
pp. 252 ◽  
Author(s):  
Bryan Thines ◽  
Emily V. Parlan ◽  
Elena C. Fulton
Keyword(s):  
Transcription Factor ◽  
Jasmonic Acid ◽  
Circadian Clock ◽  
Fungal Pathogens ◽  
Future Research ◽  
Biotic Stresses ◽  
Clock Proteins ◽  
Jaz Proteins ◽  
Multiple Levels ◽  
Ja Signaling

Plants experience specific stresses at particular, but predictable, times of the day. The circadian clock is a molecular oscillator that increases plant survival by timing internal processes to optimally match these environmental challenges. Clock regulation of jasmonic acid (JA) action is important for effective defenses against fungal pathogens and generalist herbivores in multiple plant species. Endogenous JA levels are rhythmic and under clock control with peak JA abundance during the day, a time when plants are more likely to experience certain types of biotic stresses. The expression of many JA biosynthesis, signaling, and response genes is transcriptionally controlled by the clock and timed through direct connections with core clock proteins. For example, the promoter of Arabidopsis transcription factor MYC2, a master regulator for JA signaling, is directly bound by the clock evening complex (EC) to negatively affect JA processes, including leaf senescence, at the end of the day. Also, tobacco ZEITLUPE, a circadian photoreceptor, binds directly to JAZ proteins and stimulates their degradation with resulting effects on JA root-based defenses. Collectively, a model where JA processes are embedded within the circadian network at multiple levels is emerging, and these connections to the circadian network suggest multiple avenues for future research.

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