scholarly journals Bacterial diversity and lipid biomarkers in sea ice and sinking particulate organic material during the melt season in the Canadian Arctic

Elem Sci Anth ◽  
2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Rémi Amiraux ◽  
Jean-François Rontani ◽  
Fabrice Armougom ◽  
Eléonore Frouin ◽  
Marcel Babin ◽  
...  
Keyword(s):  
Organic Matter ◽  
Sea Ice ◽  
Organic Material ◽  
Early Stage ◽  
Canadian Arctic ◽  
Carbon Fluxes ◽  
Enzymatic Activities ◽  
Ice Melt ◽  
Sinking Particles ◽  
Potential Biomarkers

The estimation of important carbon fluxes in a changing Arctic environment remains a challenge, one that could benefit from the development of biomarkers that distinguish between sympagic (ice-associated) and pelagic organic material. Products of 10S-DOX-like lipoxygenase and fatty acid cis-trans isomerase (CTI) activity of bacteria attached to sympagic particulate organic matter (POM) were proposed previously as potential biomarkers of the contribution of sympagic biota to carbon fluxes to the seafloor. To date, neither the bacteria involved in such enzymatic activities nor the detection of these potential biomarkers at their presumed source (i.e., sea ice) has been investigated. Here, we determined and compared the diversity of prokaryotic communities (based on operational taxonomic units) attached to sea ice POM and under-ice sinking particles during an early stage of ice melt (brine drainage) in Baffin Bay (Canadian Arctic). Based on a time series of biodiversity analyses and the quantification of lipid tracers of these two bacterial enzymatic activities, we suggest that CTI-active bacteria, exposed to hypersaline stress, are attached to algal POM just above bottom sea ice and released into the water column following brine drainage. In contrast, bacteria attached to sinking particles and exhibiting 10S-DOX-like lipoxygenase activity are suggested to come from the bottommost layer of sea ice, where they may play a role in the detoxification of algae-produce free fatty acids. These results provide a refined view of the potential use of products of CTI activity as specific biomarkers of sympagic organic matter.

scholarly journals Evidence for significant protein-like dissolved organic matter accumulation in Sea of Okhotsk sea ice

2015 ◽  
Vol 56 (69) ◽  
pp. 1-8 ◽  
Author(s):  
Mats A. Granskog ◽  
Daiki Nomura ◽  
Susann Müller ◽  
Andreas Krell ◽  
Takenobu Toyota ◽  
...  
Keyword(s):  
Organic Matter ◽  
Dissolved Organic Matter ◽  
Sea Ice ◽  
Surface Waters ◽  
Sea Of Okhotsk ◽  
Sea Water ◽  
First Year ◽  
Ice Melt ◽  
Ultraviolet Range ◽  
Considerable Excess

AbstractAbsorption and fluorescence of chromophoric dissolved organic matter (CDOM) in sea ice and surface waters in the southern Sea of Okhotsk was examined. Sea-water CDOM had featureless absorption increasing exponentially with shorter wavelengths. Sea ice showed distinct absorption peaks in the ultraviolet, especially in younger ice. Older first-year sea ice had relatively flat absorption spectra in the ultraviolet range. Parallel factor analysis (PARAFAC) identified five fluorescent CDOM components, two humic-like and three protein-like. Sea water was largely governed by humic-like fluorescence. In sea ice, protein-like fluorescence was found in considerable excess relative to sea water. The accumulation of protein-like CDOM fluorescence in sea ice is likely a result of biological activity within the ice. Nevertheless, sea ice does not contribute excess CDOM during melt, but the material released will be of different composition than that present in the underlying waters. Thus, at least transiently, the CDOM introduced during sea-ice melt might provide a more labile source of fresher protein-like DOM to surface waters in the southern Sea of Okhotsk.

scholarly journals Characteristics of Chromophoric and Fluorescent Dissolved Organic Matter in the Nordic Seas

2018 ◽  
Author(s):  
Anna Makarewicz ◽  
Piotr Kowalczuk ◽  
Sławomir Sagan ◽  
Mats A. Granskog ◽  
Alexey K. Pavlov ◽  
...  
Keyword(s):  
Organic Matter ◽  
Dissolved Organic Matter ◽  
Sea Ice ◽  
Chlorophyll A ◽  
Saline Water ◽  
Atlantic Water ◽  
Nordic Seas ◽  
Ice Melt ◽  
Fluorescent Dissolved Organic Matter ◽  
West Spitsbergen

Abstract. Optical properties of Chromophoric (CDOM) and Fluorescent Dissolved Organic Matter (FDOM) were characterized in the Nordic Seas including the West Spitsbergen Shelf during June–July of 2013, 2014 and 2015. The CDOM absorption coefficient at 350 nm, aCDOM(350) showed significant interannual variation. In 2013, the highest average aCDOM(350) values (aCDOM = 0.30 ± 0.12 m−1) were observed due to the influence of cold and low–saline water from the Sørkapp Current in the southern part of West Spitsbergen Shelf. In 2014, aCDOM(350) values were significantly lower than in 2013 (av. aCDOM(350) = 0.14 ± 0.06 m−1), which was associated with the dominance of warm and saline Atlantic Water (AW) in the region, while in 2015 intermediate CDOM absorption (av. aCDOM(350) = 0.19 ± 0.05 m−1) was observed. In situ measurement of three FDOM components revealed that protein–like FDOM dominated and concentration of marine and terrestrial humic–like DOM were very low and its distribution were generally vertically homogenous in the upper ocean (0–100 m). Fluorescence of terrestrial and marine humic–like FDOM decreased in surface waters (0–15 m) near the sea–ice edge by dilution of oceanic waters by sea–ice melt water. The vertical distribution of protein–like FDOM was characterized by a prominent sub–surface maximum that matched the subsurface chlorophyll a maximum and was observed all across the study area. The highest protein–like FDOM fluorescence was observed in the Norwegian Sea in the core of warm AW. There was a significant relationship between the protein–like fluorescence and chlorophyll a fluorescence (R2 = 0.65, p 

Sea ice melt and meteoric water distributions in Nares Strait, Baffin Bay, and the Canadian Arctic Archipelago

2010 ◽  
Vol 68 (6) ◽  
pp. 767-798 ◽  
Author(s):  
Matthew B. Alkire ◽  
Kelly K. Falkner ◽  
Timothy Boyd ◽  
Robie W. Macdonald
Keyword(s):  
Sea Ice ◽  
Meteoric Water ◽  
Canadian Arctic ◽  
Canadian Arctic Archipelago ◽  
Baffin Bay ◽  
Ice Melt ◽  
Nares Strait

scholarly journals Stress factors resulting from the Arctic vernal sea-ice melt

Elem Sci Anth ◽  
2020 ◽  
Vol 8 (1) ◽  
Author(s):  
Rémi Amiraux ◽  
Christopher Burot ◽  
Patricia Bonin ◽  
Guillaume Massé ◽  
Sophie Guasco ◽  
...  
Keyword(s):  
Time Series ◽  
Sea Ice ◽  
Salinity Stress ◽  
Bacterial Activity ◽  
The Arctic ◽  
Algal Production ◽  
Isomerase Activity ◽  
Ice Melt ◽  
Sinking Particles ◽  
Algal Material

During sea-ice melt in the Arctic, primary production by sympagic (sea-ice) algae can be exported efficiently to the seabed if sinking rates are rapid and activities of associated heterotrophic bacteria are limited. Salinity stress due to melting ice has been suggested to account for such low bacterial activity. We further tested this hypothesis by analyzing samples of sea ice and sinking particles collected from May 18 to June 29, 2016, in western Baffin Bay as part of the Green Edge project. We applied a method not previously used in polar regions—quantitative PCR coupled to the propidium monoazide DNA-binding method—to evaluate the viability of bacteria associated with sympagic and sinking algae. We also measured cis-trans isomerase activity, known to indicate rapid bacterial response to salinity stress in culture studies, as well as free fatty acids known to be produced by algae as bactericidal compounds. The viability of sympagic-associated bacteria was strong in May (only approximately 10% mortality of total bacteria) and weaker in June (average mortality of 43%; maximum of 75%), with instances of elevated mortality in sinking particle samples across the time series (up to 72%). Short-term stress reflected by cis-trans isomerase activity was observed only in samples of sinking particles collected early in the time series. Following snow melt, however, and saturating levels of photosynthetically active radiation in June, we observed enhanced ice-algal production of bactericidal compounds (free palmitoleic acid; up to 4.8 mg L–1). We thus suggest that protection of sinking sympagic material from bacterial degradation early in a melt season results from low bacterial activity due to salinity stress, while later in the season, algal production of bactericidal compounds induces bacterial mortality. A succession of bacterial stressors during Arctic ice melt helps to explain the efficient export of sea-ice algal material to the seabed.

scholarly journals Dimethylsulfide dynamics in first-year sea ice melt ponds in the Canadian Arctic Archipelago

2017 ◽  
Author(s):  
Margaux Gourdal ◽  
Martine Lizotte ◽  
Guillaume Massé ◽  
Michel Gosselin ◽  
Michael Scarratt ◽  
...  
Keyword(s):  
Sea Ice ◽  
Canadian Arctic ◽  
The Arctic ◽  
First Year ◽  
Canadian Arctic Archipelago ◽  
Ice Melt ◽  
Melt Pond ◽  
Potential Source ◽  
Melt Ponds ◽  
Cover Up

Abstract. Melt pond formation is a natural seasonal pan-Arctic process. During the thawing season, melt ponds may cover up to 90 % of the Arctic first year sea ice (FYI) and 15 to 25 % of the multi-year sea ice (MYI). These pools of water lying at the surface of the sea-ice cover are habitats for microorganisms and represent a potential source of the biogenic gas dimethylsulfide (DMS) for the atmosphere. Here we report on the concentrations and dynamics of DMS in nine melt ponds sampled in July 2014 in the Eastern Canadian Arctic. DMS concentrations were under the detection limit (

scholarly journals Large Diversity in Nitrogen- and Sulfur-Containing Compatible Solute Profiles in Polar and Temperate Diatoms

2020 ◽  
Vol 60 (6) ◽  
pp. 1401-1413
Author(s):  
H M Dawson ◽  
K R Heal ◽  
A Torstensson ◽  
L T Carlson ◽  
A E Ingalls ◽  
...  
Keyword(s):  
Organic Matter ◽  
Sea Ice ◽  
Algal Blooms ◽  
Dimethyl Sulfide ◽  
Compatible Solutes ◽  
Diatom Species ◽  
Variable Environment ◽  
Ice Melt ◽  
Isethionic Acid ◽  
Sulfur Containing

Synopsis Intense bottom-ice algal blooms, often dominated by diatoms, are an important source of food for grazers, organic matter for export during sea ice melt, and dissolved organic carbon. Sea-ice diatoms have a number of adaptations, including accumulation of compatible solutes, that allows them to inhabit this highly variable environment characterized by extremes in temperature, salinity, and light. In addition to protecting them from extreme conditions, these compounds present a labile, nutrient-rich source of organic matter, and include precursors to climate active compounds (e.g., dimethyl sulfide [DMS]), which are likely regulated with environmental change. Here, intracellular concentrations of 45 metabolites were quantified in three sea-ice diatom species and were compared to two temperate diatom species, with a focus on compatible solutes and free amino acid pools. There was a large diversity of metabolite concentrations between diatoms with no clear pattern identifiable for sea-ice species. Concentrations of some compatible solutes (isethionic acid, homarine) approached 1 M in the sea-ice diatoms, Fragilariopsis cylindrus and Navicula cf. perminuta, but not in the larger sea-ice diatom, Nitzschia lecointei or in the temperate diatom species. The differential use of compatible solutes in sea-ice diatoms suggests different adaptive strategies and highlights which small organic compounds may be important in polar biogeochemical cycles.

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