Metabolism of Benzo(a)pyrene by a Mixed Function Oxygenase System in Blue Crabs,

2008 ◽  
pp. 233-233-8
Author(s):  
RF Lee
Keyword(s):  
Blue Crabs ◽  
Mixed Function Oxygenase

Reactions of Blue Crabs to Low pH

1978 ◽  
Vol 107 (6) ◽  
pp. 868-871 ◽  
Author(s):  
William W. Reynolds ◽  
Martha E. Casterlin ◽  
Roger A. Laughlin ◽  
Robert J. Livingston ◽  
Claude R. Cripe
Keyword(s):  
Low Ph ◽  
Blue Crabs

Effects of Pulp and Paper Mill Effluents on Reproduction, Bile Conjugates and Liver MFO (Mixed Function Oxygenase) Activity in Fish at Southern Lake Saimaa, Finland

1999 ◽  
Vol 40 (11-12) ◽  
pp. 109-114 ◽  
Author(s):  
Aarno Karels ◽  
Markus Soimasuo ◽  
Aimo Oikari
Keyword(s):  
Endocrine Disrupting Compounds ◽  
Paper Mill ◽  
Erod Activity ◽  
Pulp And Paper ◽  
Coregonus Lavaretus ◽  
Pulp And Paper Mill ◽  
Paper Mill Effluents ◽  
Mixed Function Oxygenase ◽  
Estradiol 17Β ◽  
Lake Saimaa

Reproductive indices like gonad size, fecundity, egg size and sex steroid levels of estradiol-17β and testosterone, vitellogenin in the blood as well as bile conjugates and liver 7-ethoxyresorufin O-deethylase (EROD) activity were studied in populations of perch (Perca fluviatilis L.) and roach (Rutilus rutilus L.) and experimentally exposed juvenile whitefish (Coregonus lavaretus L. s.l.) at the Southern Lake Saimaa (S.E. Finland). Our studies showed that the introduction of elemental chlorine free (ECF) bleaching and secondary treatment of effluents in a modern activated sludge plant at the mill in 1992 have substantially reduced the exposure of feral and caged fish to organochlorines. The liver EROD activity was noticeably lower in feral and caged fish near the mill indicating lesser impacts on the liver mixed function oxygenase (MFO) system. However, at the mill site, liver EROD activies in feral and caged fish still tend to be one to four times higher than at the reference sites. Significantly decreased plasma estradiol-17β and testosterone concentrations in perch and roach in the period of development of the gonads (autumn and winter) indicate that there were endocrine disrupting compounds present in the lake receiving ECF pulp and paper mill effluents. Cause-effect relationships, however, are difficult to establish.

A Sensitive Method for Assay of Mixed-Function Oxygenase (p-450 complex) in Cell Culture

1988 ◽  
Vol 15 (3) ◽  
pp. 219-223
Author(s):  
Jørgen Clausen ◽  
Søren Achim Nielsen
Keyword(s):  
Human Lymphocytes ◽  
Cellular Systems ◽  
Metabolic Effects ◽  
Assay Method ◽  
Related Family ◽  
Oxygenase Activity ◽  
Metabolic Transformation ◽  
Mixed Function Oxygenase

The mixed-function oxygenase system involved in the metabolism of drugs and xenobiotics has been extensively studied in various animal species and in various organs (1). It is now apparent that in humans the p-450 complex is one representative of a related family, expressed by 13 c-DNA genes showing approximately 36% similarity between the different subfamilies (2). In order to compare the in vivo and in vitro metabolic effects of drugs and xenobiotics, the induction capabilities of the mixed-function oxygenase must be known. The most sensitive non-isotopic assay system for determination of mixed-function oxygenase activity is the method of Nebert & Gelboin (3,4), which is based on the metabolic transformation of benzo-(a)-pyrene to its fluorescent hydroxyl derivatives (5). However, the levels of the mixed-function oxygenase enzymes in different cellular systems show great variations, with the highest activities in liver cells. Therefore, in order to use human lymphocytes and other cellular systems with low mixed-function oxygenase activities, the assay method for determining oxygenase activity must have the highest possible sensitivity. The present communication is devoted to a study aimed at increasing the sensitivity of Nebert & Gelboin's methods for assay of mixed-function oxygenase subfamilies using benzo-(a)-pyrene as a substrate.

Comparing two types of internal tags in juvenile blue crabs

2004 ◽  
Vol 67 (3) ◽  
pp. 265-274 ◽  
Author(s):  
Jana L.D. Davis ◽  
Alicia C. Young-Williams ◽  
Anson H. Hines ◽  
Oded Zmora
Keyword(s):  
Blue Crabs
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